(Circular) (Six-base) MAPSORT of: pFN21AE1684.seq Check: 9982 from: 1 to: 5293 pFN21AE1684 5293 bp With 182 enzymes: SgfI * July 6, 2012 17:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2251 2251 Size: 5293 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3103 278 Size: 53 83 186 936 1567 2468 Fragments arranged by size: 2468 1567 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2636 1937 Size: 699 4594 Acc65I G'GTAC_C Cuts at: 2614 2614 Size: 5293 AclI AA'CG_TT Cuts at: 1563 3420 3793 1563 Size: 1857 373 3063 Fragments arranged by size: 3063 1857 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2222 2330 3356 4740 5008 1632 Size: 590 108 1026 1384 268 1917 Fragments arranged by size: 1917 1384 1026 590 268 108 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5096 AflIII A'CryG_T Cuts at: 1203 4192 5169 1203 Size: 2989 977 1327 Fragments arranged by size: 2989 1327 977 AgeI A'CCGG_T Cuts at: 4124 4124 Size: 5293 AhdI GACnn_n'nnGTC Cuts at: 4022 4022 Size: 5293 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5293 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2042 2128 1184 Size: 858 86 4349 Fragments arranged by size: 4349 858 86 AlwNI CAG_nnn'CTG Cuts at: 2072 2152 2241 3036 4608 2072 Size: 80 89 795 1572 2757 Fragments arranged by size: 2757 1572 795 89 80 ApaI G_GGCC'C Cuts at: 2439 2439 Size: 5293 ApaLI G'TGCA_C Cuts at: 3350 4506 3350 Size: 1156 4137 ApoI r'AATT_y Cuts at: 1493 2602 2828 2980 5089 1493 Size: 1109 226 152 2109 1697 Fragments arranged by size: 2109 1697 1109 226 152 AseI AT'TA_AT Cuts at: 160 1032 3847 160 Size: 872 2815 1606 Fragments arranged by size: 2815 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5293 AvaI C'yCGr_G Cuts at: 1870 1943 2445 2513 2618 1870 Size: 73 502 68 105 4545 Fragments arranged by size: 4545 502 105 73 68 BamHI G'GATC_C Cuts at: 2623 2990 2623 Size: 367 4926 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2614 4069 618 Size: 325 217 627 827 1455 1842 Fragments arranged by size: 1842 1455 827 627 325 217 BanII G_rGCy'C Cuts at: 1391 2095 2439 2612 1391 Size: 704 344 173 4072 Fragments arranged by size: 4072 704 344 173 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4322 BbvCI CC'TCA_GC Cuts at: 2421 2421 Size: 5293 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3484 3518 1903 Size: 34 1547 34 3678 Fragments arranged by size: 3678 1547 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2423 3185 4395 2423 Size: 762 1210 3321 Fragments arranged by size: 3321 1210 762 BclI T'GATC_A Cuts at: 1548 1548 Size: 5293 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3904 136 Size: 107 122 71 631 605 2232 1525 Fragments arranged by size: 2232 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5288 5288 Size: 5293 BlpI GC'TnA_GC Cuts at: 2292 2710 2292 Size: 418 4875 Bme1580I G_kGCm'C Cuts at: 1790 2439 3354 4510 1790 Size: 649 915 1156 2573 Fragments arranged by size: 2573 1156 915 649 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5293 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2119 3024 3982 Size: 416 53 83 399 24 702 905 958 Cuts at: 3982 442 Size: 1753 Fragments arranged by size: 1753 958 905 702 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5293 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2082 2339 2462 3953 1239 Size: 843 257 123 1491 2579 Fragments arranged by size: 2579 1491 843 257 123 Bpu10I CC'TnA_GC Cuts at: 2421 2421 Size: 5293 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3418 4283 4581 3418 Size: 865 298 4130 Fragments arranged by size: 4130 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2550 3956 882 Size: 617 1051 1406 2219 Fragments arranged by size: 2219 1406 1051 617 BsaAI yAC'GTr Cuts at: 493 1204 3084 493 Size: 711 1880 2702 Fragments arranged by size: 2702 1880 711 BsaBI GATnn'nnATC Cuts at: 5287 5287 Size: 5293 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3100 3482 4117 275 Size: 53 83 186 936 1567 382 635 1451 Fragments arranged by size: 1567 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3726 4124 4398 4545 4956 3726 Size: 398 274 147 411 4063 Fragments arranged by size: 4063 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5068 Fragments arranged by size: 5068 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2537 1186 Size: 1351 3942 BseYI C'CCAG_C Cuts at: 2341 2397 4496 4992 2341 Size: 56 2099 496 2642 Fragments arranged by size: 2642 2099 496 56 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2366 2453 1163 Size: 573 630 87 4003 Fragments arranged by size: 4003 630 573 87 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2743 3504 4532 1540 Size: 275 186 742 761 1028 2301 Fragments arranged by size: 2301 1028 761 742 275 186 BsiHKAI G_wGCw'C Cuts at: 3354 3439 4510 3354 Size: 85 1071 4137 Fragments arranged by size: 4137 1071 85 BsmI GAATG_Cn' Cuts at: 2804 2897 2804 Size: 93 5200 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2095 2439 2612 3354 3439 4510 1391 Size: 399 305 344 173 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 344 305 173 85 BspEI T'CCGG_A Cuts at: 4956 4956 Size: 5293 BspHI T'CATG_A Cuts at: 3182 4912 3182 Size: 1730 3563 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2251 2648 844 Size: 1407 397 3489 Fragments arranged by size: 3489 1407 397 BsrBI CCG'CTC Cuts at: 1394 1823 2220 3180 1394 Size: 429 397 960 3507 Fragments arranged by size: 3507 960 429 397 BsrDI GCAATG_nn' Cuts at: 65 1248 3788 3962 65 Size: 1183 2540 174 1396 Fragments arranged by size: 2540 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2472 3937 4124 1700 Size: 110 662 1465 187 2869 Fragments arranged by size: 2869 1465 662 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5293 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5293 BssSI C'ACGA_G Cuts at: 3353 4365 3353 Size: 1012 4281 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5293 BstBI TT'CG_AA Cuts at: 4097 4097 Size: 5293 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5293 BstYI r'GATC_y Cuts at: 1972 2623 2990 3375 3392 4844 4930 4942 5288 Size: 651 367 385 17 1452 86 12 346 Cuts at: 5288 1972 Size: 1977 Fragments arranged by size: 1977 1452 651 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2249 2249 Size: 5293 BtgI C'CryG_G Cuts at: 513 1065 2086 2277 2427 513 Size: 552 1021 191 150 3379 Fragments arranged by size: 3379 1021 552 191 150 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4554 Fragments arranged by size: 4554 683 56 BtsI GCAGTG_nn' Cuts at: 2052 2809 3603 3623 2052 Size: 757 794 20 3722 Fragments arranged by size: 3722 794 757 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5293 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5258 DraI TTT'AAA Cuts at: 2597 2597 Size: 5293 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2588 4300 809 Size: 507 1272 1712 1802 Fragments arranged by size: 1802 1712 1272 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2740 3629 Size: 54 1425 50 275 18 210 700 889 Cuts at: 3629 5258 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 700 275 210 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2740 1537 Size: 275 928 4090 Fragments arranged by size: 4090 928 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2181 2234 2543 3223 1353 Size: 828 53 309 680 3423 Fragments arranged by size: 3423 828 680 309 53 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2531 3880 4254 4400 387 Size: 1064 138 942 1349 374 146 1280 Fragments arranged by size: 1349 1280 1064 942 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2082 2222 2330 2339 2462 3356 3953 Size: 393 450 140 108 9 123 894 597 Cuts at: 3953 4740 5008 1239 Size: 787 268 1524 Fragments arranged by size: 1524 894 787 597 450 393 268 140 123 108 9 EcoNI CCTnn'n_nnAGG Cuts at: 2164 2164 Size: 5293 EcoO109I rG'GnC_Cy Cuts at: 2238 2245 2435 2442 2485 2581 2238 Size: 7 190 7 43 96 4950 Fragments arranged by size: 4950 190 96 43 7 7 EcoRI G'AATT_C Cuts at: 2602 2980 2602 Size: 378 4915 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2210 2210 Size: 5293 FspI TGC'GCA Cuts at: 3799 3799 Size: 5293 HaeII r_GCGC'y Cuts at: 1678 2275 4440 1678 Size: 597 2165 2531 Fragments arranged by size: 2531 2165 597 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3934 3966 4008 4040 1373 Size: 32 135 32 2362 32 42 32 2626 Fragments arranged by size: 2626 2362 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2637 2883 669 Size: 988 281 699 246 3079 Fragments arranged by size: 3079 988 699 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5293 HpaI GTT'AAC Cuts at: 2883 2883 Size: 5293 KpnI G_GTAC'C Cuts at: 2618 2618 Size: 5293 MfeI C'AATT_G Cuts at: 2892 2892 Size: 5293 MluI A'CGCG_T Cuts at: 5169 5169 Size: 5293 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4407 4591 1305 Size: 3102 184 2007 Fragments arranged by size: 3102 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3471 Fragments arranged by size: 3471 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2024 3251 3610 3769 518 Size: 648 858 1227 359 159 2042 Fragments arranged by size: 2042 1227 858 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2088 2257 2330 2577 2709 3386 4534 4779 Size: 482 169 73 247 132 677 1148 245 Cuts at: 4779 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 482 247 245 169 132 73 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4741 NdeI CA'TA_TG Cuts at: 387 387 Size: 5293 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5293 NotI GC'GGCC_GC Cuts at: 2740 2740 Size: 5293 NspI r_CATG'y Cuts at: 1132 1229 2023 2651 4196 1132 Size: 97 794 628 1545 2229 Fragments arranged by size: 2229 1545 794 628 97 PasI CC'CwG_GG Cuts at: 1348 2347 1348 Size: 999 4294 PciI A'CATG_T Cuts at: 4192 4192 Size: 5293 PflMI CCAn_nnn'nTGG Cuts at: 1108 2241 1108 Size: 1133 4160 PfoI T'CCnGG_A Cuts at: 2097 2478 2097 Size: 381 4912 PmeI GTTT'AAAC Cuts at: 2597 2597 Size: 5293 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3362 3394 4875 4907 3362 Size: 32 1481 32 3748 Fragments arranged by size: 3748 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2238 2245 2442 2581 2238 Size: 7 197 139 4950 Fragments arranged by size: 4950 197 139 7 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5293 PsiI TTA'TAA Cuts at: 2863 2863 Size: 5293 PspOMI G'GGCC_C Cuts at: 2435 2435 Size: 5293 PspXI vC'TCGA_Gb Cuts at: 2513 2513 Size: 5293 PstI C_TGCA'G Cuts at: 830 2157 2262 2645 830 Size: 1327 105 383 3478 Fragments arranged by size: 3478 1327 383 105 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5293 PvuII CAG'CTG Cuts at: 2257 2257 Size: 5293 SacII CC_GC'GG Cuts at: 2089 2089 Size: 5293 SalI G'TCGA_C Cuts at: 1936 2635 1936 Size: 699 4594 SbfI CC_TGCA'GG Cuts at: 2645 2645 Size: 5293 ScaI AGT'ACT Cuts at: 3541 5253 3541 Size: 1712 3581 SfcI C'TryA_G Cuts at: 826 1045 2153 2258 2641 3776 4457 4648 826 Size: 219 1108 105 383 1135 681 191 1471 Fragments arranged by size: 1471 1135 1108 681 383 219 191 105 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5293 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5293 SmaI CCC'GGG Cuts at: 1872 2447 2620 1872 Size: 575 173 4545 Fragments arranged by size: 4545 575 173 SmlI C'TyrA_G Cuts at: 820 1017 1943 2513 3397 4298 4560 820 Size: 197 926 570 884 901 262 1553 Fragments arranged by size: 1553 926 901 884 570 262 197 SnaBI TAC'GTA Cuts at: 493 3084 493 Size: 2591 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5293 SphI G_CATG'C Cuts at: 1132 2023 2651 1132 Size: 891 628 3774 Fragments arranged by size: 3774 891 628 SspI AAT'ATT Cuts at: 5 52 3217 5 Size: 47 3165 2081 Fragments arranged by size: 3165 2081 47 StuI AGG'CCT Cuts at: 1517 2159 2519 1517 Size: 642 360 4291 Fragments arranged by size: 4291 642 360 StyI C'CwwG_G Cuts at: 513 1065 2732 513 Size: 552 1667 3074 Fragments arranged by size: 3074 1667 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3320 3337 3490 3675 4120 325 Size: 1255 1740 17 153 185 445 1498 Fragments arranged by size: 1740 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3539 5251 96 Size: 274 80 33 51 1443 1562 1712 138 Fragments arranged by size: 1712 1562 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2198 2230 5253 5285 2198 Size: 32 3023 32 2206 Fragments arranged by size: 3023 2206 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5293 XbaI T'CTAG_A Cuts at: 2629 2629 Size: 5293 XhoI C'TCGA_G Cuts at: 1943 2513 1943 Size: 570 4723 XmaI C'CCGG_G Cuts at: 1870 2445 2618 1870 Size: 575 173 4545 Fragments arranged by size: 4545 575 173 XmnI GAAnn'nnTTC Cuts at: 3422 3422 Size: 5293 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3101 276 Size: 53 83 186 936 1567 2468 Fragments arranged by size: 2468 1567 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI