################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BbvCI BciVI BclI BfrI BglII BlnI BsaBI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MroI MslI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp509I Tth111I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AocI 421 CCTGAGG CC^T[ACGT]AGG BalI 479 TGGCCA TGG^CCA BamHI 675 GGATCC G^GATCC BbeI 720 GGCGCC GGCGC^C BbrPI 1149 CACGTG CAC^GTG BbsI 367 GAAGAC GAAGAC(2/6) BcgI 891 CGAGCGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 85 CTAG C^TAG BglI 802 GCCCTGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 367 GAAGAC GAAGAC(2/6) BsmBI 694 GAGACG CGTCTC(1/5) BsrBI 304 CCGCTC CCGCTC(-3/-3) BsrDI 1173 CATTGC GCAATG(2/0) BssSI 889 CACGAG CACGAG(-5/-1) Bsu36I 421 CCTGAGG CC^T[ACGT]AGG Eco81I 421 CCTGAGG CC^T[ACGT]AGG EcoT14I 1318 CCATGG C^C[AT][AT]GG KasI 720 GGCGCC G^GCGCC MaeI 85 CTAG C^TAG MluNI 479 TGGCCA TGG^CCA MscI 479 TGGCCA TGG^CCA MseI 1310 TTAA T^TAA MspA1I 1075 CAGCTG C[AC]G^C[GT]G NarI 720 GGCGCC GG^CGCC NcoI 1318 CCATGG C^CATGG PaeR7I 983 CTCGAG C^TCGAG PmaCI 1149 CACGTG CAC^GTG PmlI 1149 CACGTG CAC^GTG PvuII 1075 CAGCTG CAG^CTG SacI 1099 GAGCTC GAGCT^C SfoI 720 GGCGCC GGC^GCC SmaI 1208 CCCGGG CCC^GGG StyI 1318 CCATGG C^C[AT][AT]GG TaqI 984 TCGA T^CGA TfiI 520 GATTC G^A[AT]TC Tru9I 1310 TTAA T^TAA TthHB8I 984 TCGA T^CGA XhoI 983 CTCGAG C^TCGAG XmaCI 1208 CCCGGG C^CCGGG XmaI 1208 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 720 GGCGCC G[GA]^CG[CT]C 1015 GACGCC AfaI 489 GTAC GT^AC 1137 GTAC ApaI 88 GGGCCC GGGCC^C 461 GGGCCC AvaI 983 CTCGAG C^[CT]CG[GA]G 1208 CCCGGG AviII 506 TGCGCA TGC^GCA 900 TGCGCA BlpI 306 GCTCAGC GC^T[ACGT]AGC 1215 GCTCAGC Bpu1102I 306 GCTCAGC GC^T[ACGT]AGC 1215 GCTCAGC BsaAI 475 TACGTG [CT]AC^GT[GA] 1149 CACGTG BsaHI 720 GGCGCC G[GA]^CG[CT]C 1015 GACGCC BsaI 184 GAGACC GGTCTC(1/5) 285 GGTCTC BsaWI 768 ACCGGA [AT]^CCGG[AT] 846 ACCGGA BsoBI 983 CTCGAG C^[CT]CG[GA]G 1208 CCCGGG BspMI 8 ACCTGC ACCTGC(4/8) 1301 ACCTGC BstXI 344 CCAGCCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 534 CCACGGGTCTGG CelII 306 GCTCAGC GC^T[ACGT]AGC 1215 GCTCAGC EaeI 479 TGGCCA [CT]^GGCC[GA] 551 CGGCCA FspI 506 TGCGCA TGC^GCA 900 TGCGCA HgaI 1015 GACGC GACGC(5/10) 1263 GACGC Hin1I 720 GGCGCC G[GA]^CG[CT]C 1015 GACGCC MaeIII 1336 GTGAC ^GT[ACGT]AC 1349 GTGAC NlaIII 250 CATG CATG^ 1319 CATG PflMI 15 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 447 CCAGTCTCTGG PleI 616 GACTC GAGTC(4/5) 749 GACTC RsaI 489 GTAC GT^AC 1137 GTAC SfaNI 509 GCATC GCATC(5/9) 884 GCATC SmlI 218 CTCAAG C^T[CT][GA]AG 983 CTCGAG Tsp45I 1336 GTGAC ^GT[GC]AC 1349 GTGAC TspRI 1246 CAGTG CA[GC]TG[ACGT][ACGT]^ 1347 CAGTG Van91I 15 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 447 CCAGTCTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AccII AciI AluI AlwI AlwNI AspHI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EarI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MboI MboII MflI MnlI MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIV PpuMI Sau3AI Sau96I ScrFI TseI XhoII