Restriction map for
pFN21AE0242
(Linear) (Six-base) MAPSORT of: KIEE0242.seq Check: 7488 from: 1 to: 1356
KIEE0242.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 550 748 1356
Size: 428 122 198 608
Fragments arranged by size:
608 428 198 122
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1190 1356
Size: 1190 166
AlwNI CAG_nnn'CTG
Cuts at: 0 112 197 238 453 523 1356
Size: 112 85 41 215 70 833
Fragments arranged by size:
833 215 112 85 70 41
ApaI G_GGCC'C
Cuts at: 0 92 465 1356
Size: 92 373 891
Fragments arranged by size:
891 373 92
AvaI C'yCGr_G
Cuts at: 0 983 1208 1356
Size: 983 225 148
Fragments arranged by size:
983 225 148
BamHI G'GATC_C
Cuts at: 0 675 1356
Size: 675 681
BanI G'GyrC_C
Cuts at: 0 578 720 1250 1356
Size: 578 142 530 106
Fragments arranged by size:
578 530 142 106
BanII G_rGCy'C
Cuts at: 0 92 465 659 862 1057 1103 1217 1293
Size: 92 373 194 203 195 46 114 76
Cuts at: 1293 1356
Size: 63
Fragments arranged by size:
373 203 195 194 114 92 76 63
46
BbeI G_GCGC'C
Cuts at: 0 724 1356
Size: 724 632
BbsI GAAGACnn'nnnn_
Cuts at: 0 374 1356
Size: 374 982
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 880 914 1356
Size: 880 34 442
Fragments arranged by size:
880 442 34
BglI GCCn_nnn'nGGC
Cuts at: 0 808 1356
Size: 808 548
BlpI GC'TnA_GC
Cuts at: 0 307 1216 1356
Size: 307 909 140
Fragments arranged by size:
909 307 140
Bme1580I G_kGCm'C
Cuts at: 0 92 465 834 1356
Size: 92 373 369 522
Fragments arranged by size:
522 373 369 92
BmgBI CAC'GTC
Cuts at: 0 429 444 1356
Size: 429 15 912
Fragments arranged by size:
912 429 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 580 712 1071 1356
Size: 202 378 132 359 285
Fragments arranged by size:
378 359 285 202 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 1356
Size: 203 1153
BsaI GGTCTCn'nnnn_
Cuts at: 0 178 291 1356
Size: 178 113 1065
Fragments arranged by size:
1065 178 113
BsaAI yAC'GTr
Cuts at: 0 477 1151 1356
Size: 477 674 205
Fragments arranged by size:
674 477 205
BsaHI Gr'CG_yC
Cuts at: 0 721 1016 1356
Size: 721 295 340
Fragments arranged by size:
721 340 295
BsaWI w'CCGG_w
Cuts at: 0 768 846 1356
Size: 768 78 510
Fragments arranged by size:
768 510 78
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 271 301 1356
Size: 271 30 1055
Fragments arranged by size:
1055 271 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 373 376 934 1356
Size: 373 3 558 422
Fragments arranged by size:
558 422 373 3
BseYI C'CCAG_C
Cuts at: 0 197 1132 1356
Size: 197 935 224
Fragments arranged by size:
935 224 197
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 978 1150 1225 1252 1356
Size: 136 842 172 75 27 104
Fragments arranged by size:
842 172 136 104 75 27
BsiHKAI G_wGCw'C
Cuts at: 0 434 768 1103 1356
Size: 434 334 335 253
Fragments arranged by size:
434 335 334 253
BsmBI CGTCTCn'nnnn_
Cuts at: 0 688 1356
Size: 688 668
Bsp1286I G_dGCh'C
Cuts at: 0 92 434 465 659 768 834 862 1057
Size: 92 342 31 194 109 66 28 195
Cuts at: 1057 1103 1217 1293 1356
Size: 46 114 76 63
Fragments arranged by size:
342 195 194 114 109 92 76 66
63 46 31 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 17 1310 1356
Size: 17 1293 46
Fragments arranged by size:
1293 46 17
BsrBI CCG'CTC
Cuts at: 0 306 1356
Size: 306 1050
BsrDI GCAATG_nn'
Cuts at: 0 1172 1356
Size: 1172 184
BsrFI r'CCGG_y
Cuts at: 0 333 732 1026 1356
Size: 333 399 294 330
Fragments arranged by size:
399 333 330 294
BssSI C'ACGA_G
Cuts at: 0 889 1356
Size: 889 467
BstXI CCAn_nnnn'nTGG
Cuts at: 0 351 541 1356
Size: 351 190 815
Fragments arranged by size:
815 351 190
BstYI r'GATC_y
Cuts at: 0 108 675 999 1107 1356
Size: 108 567 324 108 249
Fragments arranged by size:
567 324 249 108 108
Bsu36I CC'TnA_GG
Cuts at: 0 422 1356
Size: 422 934
BtgI C'CryG_G
Cuts at: 0 534 554 1318 1356
Size: 534 20 764 38
Fragments arranged by size:
764 534 38 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 495 1356
Size: 495 861
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1137 1172 1356
Size: 1137 35 184
Fragments arranged by size:
1137 184 35
EaeI y'GGCC_r
Cuts at: 0 479 551 1356
Size: 479 72 805
Fragments arranged by size:
805 479 72
EarI CTCTTCn'nnn_
Cuts at: 0 350 404 1322 1356
Size: 350 54 918 34
Fragments arranged by size:
918 350 54 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1275 1356
Size: 1275 81
EcoICRI GAG'CTC
Cuts at: 0 1101 1356
Size: 1101 255
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 428 550 580 712 748 1071 1356
Size: 202 226 122 30 132 36 323 285
Fragments arranged by size:
323 285 226 202 132 122 36 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 610 624 949 1356
Size: 610 14 325 407
Fragments arranged by size:
610 407 325 14
EcoO109I rG'GnC_Cy
Cuts at: 0 88 418 461 529 546 649 682 820
Size: 88 330 43 68 17 103 33 138
Cuts at: 820 1356
Size: 536
Fragments arranged by size:
536 330 138 103 88 68 43 33
17
FspI TGC'GCA
Cuts at: 0 508 902 1356
Size: 508 394 454
Fragments arranged by size:
508 454 394
FspAI rTGC'GCAy
Cuts at: 0 508 1356
Size: 508 848
HaeII r_GCGC'y
Cuts at: 0 724 897 943 1033 1165 1356
Size: 724 173 46 90 132 191
Fragments arranged by size:
724 191 173 132 90 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 237 269 1356
Size: 237 32 1087
Fragments arranged by size:
1087 237 32
KasI G'GCGC_C
Cuts at: 0 720 1356
Size: 720 636
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 1356
Size: 156 1200
MscI TGG'CCA
Cuts at: 0 481 1356
Size: 481 875
MspA1I CmG'CkG
Cuts at: 0 1077 1356
Size: 1077 279
NaeI GCC'GGC
Cuts at: 0 335 734 1028 1356
Size: 335 399 294 328
Fragments arranged by size:
399 335 328 294
NarI GG'CG_CC
Cuts at: 0 721 1356
Size: 721 635
NcoI C'CATG_G
Cuts at: 0 1318 1356
Size: 1318 38
NgoMIV G'CCGG_C
Cuts at: 0 333 732 1026 1356
Size: 333 399 294 330
Fragments arranged by size:
399 333 330 294
PasI CC'CwG_GG
Cuts at: 0 145 1005 1356
Size: 145 860 351
Fragments arranged by size:
860 351 145
PflMI CCAn_nnn'nTGG
Cuts at: 0 21 453 1356
Size: 21 432 903
Fragments arranged by size:
903 432 21
PfoI T'CCnGG_A
Cuts at: 0 596 1356
Size: 596 760
PmlI CAC'GTG
Cuts at: 0 1151 1356
Size: 1151 205
PpuMI rG'GwC_Cy
Cuts at: 0 529 546 649 1356
Size: 529 17 103 707
Fragments arranged by size:
707 529 103 17
PspOMI G'GGCC_C
Cuts at: 0 88 461 1356
Size: 88 373 895
Fragments arranged by size:
895 373 88
PspXI vC'TCGA_Gb
Cuts at: 0 983 1356
Size: 983 373
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 160 192 1356
Size: 160 32 1164
Fragments arranged by size:
1164 160 32
PvuII CAG'CTG
Cuts at: 0 1077 1356
Size: 1077 279
SacI G_AGCT'C
Cuts at: 0 1103 1356
Size: 1103 253
SanDI GG'GwC_CC
Cuts at: 0 529 546 1356
Size: 529 17 810
Fragments arranged by size:
810 529 17
SfoI GGC'GCC
Cuts at: 0 722 1356
Size: 722 634
SmaI CCC'GGG
Cuts at: 0 1210 1356
Size: 1210 146
SmlI C'TyrA_G
Cuts at: 0 218 983 1356
Size: 218 765 373
Fragments arranged by size:
765 373 218
StyI C'CwwG_G
Cuts at: 0 1318 1356
Size: 1318 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 282 1051 1356
Size: 282 769 305
Fragments arranged by size:
769 305 282
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 271 303 1356
Size: 271 32 1053
Fragments arranged by size:
1053 271 32
XhoI C'TCGA_G
Cuts at: 0 983 1356
Size: 983 373
XmaI C'CCGG_G
Cuts at: 0 1208 1356
Size: 1208 148
Enzymes that do cut:
AcuI AlfI AlwNI ApaI AvaI BamHI BanI BanII
BbeI BbsI BcgI BglI BlpI Bme1580I BmgBI BpmI
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssSI BstXI BstYI Bsu36I BtgI BtgZI CspCI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI
HaeII Hin4I KasI MmeI MscI MspA1I NaeI NarI
NcoI NgoMIV PasI PflMI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII SacI SanDI SfoI SmaI SmlI
StyI TaqII TstI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbvCI BciVI BclI BfrBI BglII
BmrI BmtI BplI Bpu10I BsaBI BsiEI BsiWI BsmI
BspEI BspHI BsrGI BssHII BstAPI BstBI BstEII BstZ17I
BtsI ClaI DraI DraIII DrdI EagI EcoRI EcoRV
FalI FseI HincII HindIII HpaI KpnI MfeI MluI
MslI NdeI NheI NotI NruI NsiI NspI PacI
PciI PmeI PpiI PshAI PsiI PstI PvuI RsrII
SacII SalI SapI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI Tth111I XbaI XcmI XmnI ZraI