################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BlnI BlpI BmyI Bpu1102I BsaAI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI EagI EclXI Eco52I Eco81I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HapII Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MfeI MluI MroI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tsp509I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AhdI 622 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 202 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 89 AGCGCT AGC^GCT AspEI 622 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 406 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 77 TGGCCA TGG^CCA BbsI 403 GAAGAC GAAGAC(2/6) BbvI 468 GCTGC GCAGC(8/12) BglI 136 GCCAACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 557 AGATCT A^GATCT BpuAI 403 GAAGAC GAAGAC(2/6) BsaBI 558 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 328 GAGACC GGTCTC(1/5) BseRI 645 CTCCTC GAGGAG(10/8) BsmBI 100 GAGACG CGTCTC(1/5) BsmFI 317 GGGAC GGGAC(10/14) BstEII 192 GGTCACC G^GT[ACGT]ACC BstPI 192 GGTCACC G^GT[ACGT]ACC BstXI 632 CCAGCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 90 GCGC GCG^C DdeI 68 CTGAG C^T[ACGT]AG DraIII 246 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 188 CCACGG C^C[GA][CT]GG Eam1105I 622 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 577 GAAGAG CTCTTC(1/4) Eco47III 89 AGCGCT AGC^GCT EcoNI 207 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 192 GGTCACC G^GT[ACGT]ACC EcoT14I 110 CCAAGG C^C[AT][AT]GG HaeII 89 AGCGCT [GA]GCGC^[CT] HgaI 102 GACGC GACGC(5/10) HhaI 90 GCGC GCG^C HinP1I 90 GCGC G^CGC Ksp632I 577 GAAGAG CTCTTC(1/4) MaeII 29 ACGT A^CGT MaeIII 193 GTCAC ^GT[ACGT]AC MamI 558 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 77 TGGCCA TGG^CCA MscI 77 TGGCCA TGG^CCA MseI 587 TTAA T^TAA MspA1I 462 CCGCTG C[AC]G^C[GT]G NlaIV 317 GGGACC GG[ACGT]^[ACGT]CC PflFI 406 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 429 CCATGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 317 GGGACCT [GA]G^G[AT]CC[CT] PshAI 186 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfcI 567 CTGTAG C^T[GA][CT]AG SmlI 43 CTCAAG C^T[CT][GA]AG StuI 344 AGGCCT AGG^CCT StyI 110 CCAAGG C^C[AT][AT]GG Tru9I 587 TTAA T^TAA TseI 468 GCTGC G^C[AT]GC Tsp45I 193 GTCAC ^GT[GC]AC TspRI 624 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 406 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 429 CCATGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 593 CCAAGAACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AluI 155 AGCT AG^CT 506 AGCT AlwI 204 GATCC GGATC(4/5) 489 GGATC AvaI 313 CTCGGG C^[CT]CG[GA]G 484 CTCGGG AvaII 318 GGACC G^G[AT]CC 375 GGACC BsmAI 100 GAGAC GTCTC(1/5) 328 GAGAC BsoBI 313 CTCGGG C^[CT]CG[GA]G 484 CTCGGG BspMI 320 ACCTGC ACCTGC(4/8) 331 ACCTGC BsrI 394 ACTGG ACTGG(1/-1) 162 CCAGT BstF5I 363 GGATG GGATG(2/0) 518 GGATG DraII 215 AGGCCCT [GA]G^G[ACGT]CC[CT] 317 GGGACCT EcoO109I 215 AGGCCCT [GA]G^G[ACGT]CC[CT] 317 GGGACCT Fnu4HI 231 GCCGC GC^[ACGT]GC 468 GCTGC FokI 363 GGATG GGATG(9/13) 518 GGATG HinfI 406 GACTC G^A[ACGT]TC 547 GAGTC ItaI 231 GCCGC GC^[ACGT]GC 468 GCTGC MwoI 136 GCCAACCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 524 GCAAATACGGC PleI 406 GACTC GAGTC(4/5) 547 GAGTC SfaNI 480 GATGC GCATC(5/9) 635 GCATC TaqI 53 TCGA T^CGA 266 TCGA TthHB8I 53 TCGA T^CGA 266 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AciI AfaI BpmI BsaJI BsiYI BslI BssKI BstNI BstYI Cac8I Cfr13I DpnII EaeI EcoRII HaeIII HphI MboI MboII MflI MnlI MvaI NdeII NlaIII RsaI Sau3AI Sau96I ScrFI XhoII