(Circular) (Six-base) MAPSORT of: pFN21AE5271.seq Check: 5806 from: 1 to: 5359 pFN21AE5271 5359 bp With 182 enzymes: SgfI * January 30, 2014 17:16 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3169 278 Size: 53 83 186 936 1633 2468 Fragments arranged by size: 2468 1633 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2702 1937 Size: 765 4594 Acc65I G'GTAC_C Cuts at: 2680 2680 Size: 5359 AclI AA'CG_TT Cuts at: 1563 3486 3859 1563 Size: 1923 373 3063 Fragments arranged by size: 3063 1923 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2177 2237 3422 4806 5074 1632 Size: 545 60 1185 1384 268 1917 Fragments arranged by size: 1917 1384 1185 545 268 60 AfeI AGC'GCT Cuts at: 2096 2096 Size: 5359 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5162 AflIII A'CryG_T Cuts at: 1203 4258 5235 1203 Size: 3055 977 1327 Fragments arranged by size: 3055 1327 977 AgeI A'CCGG_T Cuts at: 4190 4190 Size: 5359 AhdI GACnn_n'nnGTC Cuts at: 2632 4088 2632 Size: 1456 3903 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5359 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5359 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2283 2315 2283 Size: 32 5327 AlwNI CAG_nnn'CTG Cuts at: 2212 3102 4674 2212 Size: 890 1572 2897 Fragments arranged by size: 2897 1572 890 ApaLI G'TGCA_C Cuts at: 3416 4572 3416 Size: 1156 4203 ApoI r'AATT_y Cuts at: 1493 2668 2894 3046 5155 1493 Size: 1175 226 152 2109 1697 Fragments arranged by size: 2109 1697 1175 226 152 AseI AT'TA_AT Cuts at: 160 1032 3913 160 Size: 872 2881 1606 Fragments arranged by size: 2881 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5359 AvaI C'yCGr_G Cuts at: 1870 1943 2318 2489 2684 1870 Size: 73 375 171 195 4545 Fragments arranged by size: 4545 375 195 171 73 BamHI G'GATC_C Cuts at: 2689 3056 2689 Size: 367 4992 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2680 4135 618 Size: 325 217 627 893 1455 1842 Fragments arranged by size: 1842 1455 893 627 325 217 BanII G_rGCy'C Cuts at: 1391 2678 1391 Size: 1287 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2415 928 Size: 971 516 3872 Fragments arranged by size: 3872 971 516 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3550 3584 1903 Size: 34 1613 34 3678 Fragments arranged by size: 3678 1613 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3251 4461 3251 Size: 1210 4149 BclI T'GATC_A Cuts at: 1548 1548 Size: 5359 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2147 3970 136 Size: 107 122 71 631 605 475 1823 1525 Fragments arranged by size: 1823 1525 631 605 475 122 107 71 BglII A'GATC_T Cuts at: 2562 5354 2562 Size: 2792 2567 BlpI GC'TnA_GC Cuts at: 2776 2776 Size: 5359 Bme1580I G_kGCm'C Cuts at: 1790 3420 4576 1790 Size: 1630 1156 2573 Fragments arranged by size: 2573 1630 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5359 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3090 4048 442 Size: 416 53 83 399 24 1673 958 1753 Fragments arranged by size: 1753 1673 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5359 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2111 2351 2560 4019 1239 Size: 872 240 209 1459 2579 Fragments arranged by size: 2579 1459 872 240 209 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2033 3484 4349 4647 2033 Size: 1451 865 298 2745 Fragments arranged by size: 2745 1451 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2327 4022 882 Size: 617 828 1695 2219 Fragments arranged by size: 2219 1695 828 617 BsaAI yAC'GTr Cuts at: 493 1204 3150 493 Size: 711 1946 2702 Fragments arranged by size: 2702 1946 711 BsaBI GATnn'nnATC Cuts at: 2567 5353 2567 Size: 2786 2573 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3166 3548 4183 275 Size: 53 83 186 936 1633 382 635 1451 Fragments arranged by size: 1633 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3792 4190 4464 4611 5022 3792 Size: 398 274 147 411 4129 Fragments arranged by size: 4129 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5134 Fragments arranged by size: 5134 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2641 1186 Size: 1455 3904 BseYI C'CCAG_C Cuts at: 2176 4562 5058 2176 Size: 2386 496 2477 Fragments arranged by size: 2477 2386 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4786 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2809 3570 4598 1540 Size: 275 186 808 761 1028 2301 Fragments arranged by size: 2301 1028 808 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3420 3505 4576 3420 Size: 85 1071 4203 Fragments arranged by size: 4203 1071 85 BsmI GAATG_Cn' Cuts at: 2870 2963 2870 Size: 93 5266 BsmBI CGTCTCn'nnnn_ Cuts at: 2099 2099 Size: 5359 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2678 3420 3505 4576 1391 Size: 399 888 742 85 1071 2174 Fragments arranged by size: 2174 1071 888 742 399 85 BspEI T'CCGG_A Cuts at: 5022 5022 Size: 5359 BspHI T'CATG_A Cuts at: 3248 4978 3248 Size: 1730 3629 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2334 2345 2714 844 Size: 1490 11 369 3489 Fragments arranged by size: 3489 1490 369 11 BsrBI CCG'CTC Cuts at: 1394 1823 3246 1394 Size: 429 1423 3507 Fragments arranged by size: 3507 1423 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3854 4028 65 Size: 1183 2606 174 1396 Fragments arranged by size: 2606 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4003 4190 1700 Size: 110 2193 187 2869 Fragments arranged by size: 2869 2193 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5359 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5359 BssSI C'ACGA_G Cuts at: 3419 4431 3419 Size: 1012 4347 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5359 BstBI TT'CG_AA Cuts at: 4163 4163 Size: 5359 BstEII G'GTnAC_C Cuts at: 1184 2197 1184 Size: 1013 4346 BstXI CCAn_nnnn'nTGG Cuts at: 2644 2644 Size: 5359 BstYI r'GATC_y Cuts at: 1972 2208 2494 2562 2689 3056 3441 3458 4910 Size: 236 286 68 127 367 385 17 1452 Cuts at: 4910 4996 5008 5354 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 286 236 127 86 68 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2193 513 Size: 552 939 189 3679 Fragments arranged by size: 3679 939 552 189 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2024 487 Size: 683 56 798 3822 Fragments arranged by size: 3822 798 683 56 BtsI GCAGTG_nn' Cuts at: 2875 3669 3689 2875 Size: 794 20 4545 Fragments arranged by size: 4545 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5359 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5324 DraI TTT'AAA Cuts at: 2663 2663 Size: 5359 DraIII CAC_nnn'GTG Cuts at: 2256 2256 Size: 5359 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4366 809 Size: 507 3050 1802 Fragments arranged by size: 3050 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2082 2294 2478 Size: 54 1425 50 275 18 252 212 184 Cuts at: 2478 2806 3695 5324 8 Size: 328 889 1629 43 Fragments arranged by size: 1629 1425 889 328 275 252 212 184 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2806 1537 Size: 275 994 4090 Fragments arranged by size: 4090 994 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2577 3289 1353 Size: 1224 712 3423 Fragments arranged by size: 3423 1224 712 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3946 4320 4466 387 Size: 1064 138 2357 374 146 1280 Fragments arranged by size: 2357 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2111 2177 2237 2351 2560 3422 4019 Size: 393 479 66 60 114 209 862 597 Cuts at: 4019 4806 5074 1239 Size: 787 268 1524 Fragments arranged by size: 1524 862 787 597 479 393 268 209 114 66 60 EcoNI CCTnn'n_nnAGG Cuts at: 2216 2216 Size: 5359 EcoO109I rG'GnC_Cy Cuts at: 2221 2323 2221 Size: 102 5257 EcoRI G'AATT_C Cuts at: 2668 3046 2668 Size: 378 4981 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2638 2638 Size: 5359 FspI TGC'GCA Cuts at: 3865 3865 Size: 5359 HaeII r_GCGC'y Cuts at: 1678 2098 4506 1678 Size: 420 2408 2531 Fragments arranged by size: 2531 2408 420 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2618 2650 4000 4032 4074 Size: 32 135 32 1046 32 1350 32 42 Cuts at: 4074 4106 1373 Size: 32 2626 Fragments arranged by size: 2626 1350 1046 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2703 2949 669 Size: 988 281 765 246 3079 Fragments arranged by size: 3079 988 765 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5359 HpaI GTT'AAC Cuts at: 2949 2949 Size: 5359 KpnI G_GTAC'C Cuts at: 2684 2684 Size: 5359 MfeI C'AATT_G Cuts at: 2958 2958 Size: 5359 MluI A'CGCG_T Cuts at: 5235 5235 Size: 5359 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2326 2481 4473 4657 1305 Size: 1021 155 1992 184 2007 Fragments arranged by size: 2007 1992 1021 184 155 MscI TGG'CCA Cuts at: 10 64 1489 1832 2084 10 Size: 54 1425 343 252 3285 Fragments arranged by size: 3285 1425 343 252 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3317 3676 3835 518 Size: 648 843 1308 359 159 2042 Fragments arranged by size: 2042 1308 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2469 2775 3452 4600 4845 1606 Size: 863 306 677 1148 245 2120 Fragments arranged by size: 2120 1148 863 677 306 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3868 Fragments arranged by size: 3868 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5359 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5359 NotI GC'GGCC_GC Cuts at: 2806 2806 Size: 5359 NspI r_CATG'y Cuts at: 1132 1229 2717 4262 1132 Size: 97 1488 1545 2229 Fragments arranged by size: 2229 1545 1488 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5359 PciI A'CATG_T Cuts at: 4258 4258 Size: 5359 PflMI CCAn_nnn'nTGG Cuts at: 1108 2440 1108 Size: 1332 4027 PmeI GTTT'AAAC Cuts at: 2663 2663 Size: 5359 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3428 3460 4941 4973 3428 Size: 32 1481 32 3814 Fragments arranged by size: 3814 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2323 2323 Size: 5359 PshAI GACnn'nnGTC Cuts at: 1613 2195 1613 Size: 582 4777 PsiI TTA'TAA Cuts at: 2929 2929 Size: 5359 PstI C_TGCA'G Cuts at: 830 2711 830 Size: 1881 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5359 SalI G'TCGA_C Cuts at: 1936 2701 1936 Size: 765 4594 SbfI CC_TGCA'GG Cuts at: 2711 2711 Size: 5359 ScaI AGT'ACT Cuts at: 3607 5319 3607 Size: 1712 3647 SfcI C'TryA_G Cuts at: 826 1045 2572 2707 3842 4523 4714 826 Size: 219 1527 135 1135 681 191 1471 Fragments arranged by size: 1527 1471 1135 681 219 191 135 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5359 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5359 SmaI CCC'GGG Cuts at: 1872 2686 1872 Size: 814 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2048 3463 4364 4626 820 Size: 197 926 105 1415 901 262 1553 Fragments arranged by size: 1553 1415 926 901 262 197 105 SnaBI TAC'GTA Cuts at: 493 3150 493 Size: 2657 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5359 SphI G_CATG'C Cuts at: 1132 2717 1132 Size: 1585 3774 SspI AAT'ATT Cuts at: 5 52 3283 5 Size: 47 3231 2081 Fragments arranged by size: 3231 2081 47 StuI AGG'CCT Cuts at: 1517 2351 1517 Size: 834 4525 StyI C'CwwG_G Cuts at: 513 1065 2004 2115 2798 513 Size: 552 939 111 683 3074 Fragments arranged by size: 3074 939 683 552 111 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3386 3403 3556 3741 4186 325 Size: 1255 1806 17 153 185 445 1498 Fragments arranged by size: 1806 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3605 5317 96 Size: 274 80 33 51 1443 1628 1712 138 Fragments arranged by size: 1712 1628 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5319 5351 5319 Size: 32 5327 Tth111I GACn'n_nGTC Cuts at: 1316 2414 1316 Size: 1098 4261 XbaI T'CTAG_A Cuts at: 2695 2695 Size: 5359 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2605 2605 Size: 5359 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5359 XmaI C'CCGG_G Cuts at: 1870 2684 1870 Size: 814 4545 XmnI GAAnn'nnTTC Cuts at: 3488 3488 Size: 5359 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3167 276 Size: 53 83 186 936 1633 2468 Fragments arranged by size: 2468 1633 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI