(Linear) (Six-base) MAPSORT of: KIEE5271.seq Check: 9694 from: 1 to: 654 KIEE5271.orf With 182 enzymes: SgfI * January 29, 2014 11:53 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 172 232 654 Size: 172 60 422 Fragments arranged by size: 422 172 60 AfeI AGC'GCT Cuts at: 0 91 654 Size: 91 563 AhdI GACnn_n'nnGTC Cuts at: 0 627 654 Size: 627 27 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 278 310 654 Size: 278 32 344 Fragments arranged by size: 344 278 32 AlwNI CAG_nnn'CTG Cuts at: 0 207 654 Size: 207 447 AvaI C'yCGr_G Cuts at: 0 313 484 654 Size: 313 171 170 Fragments arranged by size: 313 171 170 BbsI GAAGACnn'nnnn_ Cuts at: 0 410 654 Size: 410 244 BglI GCCn_nnn'nGGC Cuts at: 0 142 654 Size: 142 512 BglII A'GATC_T Cuts at: 0 557 654 Size: 557 97 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 106 346 555 654 Size: 106 240 209 99 Fragments arranged by size: 240 209 106 99 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 28 654 Size: 28 626 BsaI GGTCTCn'nnnn_ Cuts at: 0 322 654 Size: 322 332 BsaBI GATnn'nnATC Cuts at: 0 562 654 Size: 562 92 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 636 654 Size: 636 18 BseYI C'CCAG_C Cuts at: 0 171 654 Size: 171 483 BsmBI CGTCTCn'nnnn_ Cuts at: 0 94 654 Size: 94 560 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 329 340 654 Size: 329 11 314 Fragments arranged by size: 329 314 11 BstEII G'GTnAC_C Cuts at: 0 192 654 Size: 192 462 BstXI CCAn_nnnn'nTGG Cuts at: 0 639 654 Size: 639 15 BstYI r'GATC_y Cuts at: 0 203 489 557 654 Size: 203 286 68 97 Fragments arranged by size: 286 203 97 68 BtgI C'CryG_G Cuts at: 0 188 654 Size: 188 466 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 19 654 Size: 19 635 DraIII CAC_nnn'GTG Cuts at: 0 251 654 Size: 251 403 EaeI y'GGCC_r Cuts at: 0 77 289 473 654 Size: 77 212 184 181 Fragments arranged by size: 212 184 181 77 EarI CTCTTCn'nnn_ Cuts at: 0 572 654 Size: 572 82 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 106 172 232 346 555 654 Size: 106 66 60 114 209 99 Fragments arranged by size: 209 114 106 99 66 60 EcoNI CCTnn'n_nnAGG Cuts at: 0 211 654 Size: 211 443 EcoO109I rG'GnC_Cy Cuts at: 0 216 318 654 Size: 216 102 336 Fragments arranged by size: 336 216 102 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 633 654 Size: 633 21 HaeII r_GCGC'y Cuts at: 0 93 654 Size: 93 561 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 613 645 654 Size: 613 32 9 Fragments arranged by size: 613 32 9 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 321 476 654 Size: 321 155 178 Fragments arranged by size: 321 178 155 MscI TGG'CCA Cuts at: 0 79 654 Size: 79 575 MspA1I CmG'CkG Cuts at: 0 464 654 Size: 464 190 PflMI CCAn_nnn'nTGG Cuts at: 0 435 654 Size: 435 219 PpuMI rG'GwC_Cy Cuts at: 0 318 654 Size: 318 336 PshAI GACnn'nnGTC Cuts at: 0 190 654 Size: 190 464 SfcI C'TryA_G Cuts at: 0 567 654 Size: 567 87 SmlI C'TyrA_G Cuts at: 0 43 654 Size: 43 611 StuI AGG'CCT Cuts at: 0 346 654 Size: 346 308 StyI C'CwwG_G Cuts at: 0 110 654 Size: 110 544 Tth111I GACn'n_nGTC Cuts at: 0 409 654 Size: 409 245 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 600 654 Size: 600 54 Enzymes that do cut: AcuI AfeI AhdI AloI AlwNI AvaI BbsI BglI BglII BpmI BpuEI BsaI BsaBI BseRI BseYI BsmBI BspMI BstEII BstXI BstYI BtgI BtgZI DraIII EaeI EarI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I MmeI MscI MspA1I PflMI PpuMI PshAI SfcI SmlI StuI StyI Tth111I XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AleI AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaAI BsaHI BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI TstI XbaI XhoI XmaI XmnI ZraI