Restriction map for
pFN21AE6281
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI BanI
BbeI BbrPI BcgI BciVI BclI
BfrI BglI BglII BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsiWI
BsmAI BsmBI BsoBI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MseI MslI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tru9I
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 652 GTCTAC GT^[AC][GT]AC
AccIII 712 TCCGGA T^CCGGA
AlwNI 410 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 72 GGTCC G^G[AT]CC
AviII 695 TGCGCA TGC^GCA
AvrII 154 CCTAGG C^CTAGG
BalI 296 TGGCCA TGG^CCA
BamHI 181 GGATCC G^GATCC
BanII 708 GAGCTC G[GA]GC[CT]^C
BbsI 358 GAAGAC GAAGAC(2/6)
BbvCI 18 GCTGAGG CCTCAGC(-5/-2)
BfaI 155 CTAG C^TAG
BlnI 154 CCTAGG C^CTAGG
BpuAI 358 GAAGAC GAAGAC(2/6)
BsaWI 712 TCCGGA [AT]^CCGG[AT]
BseAI 712 TCCGGA T^CCGGA
BsgI 396 CTGCAC GTGCAG(16/14)
BsmFI 328 GTCCC GGGAC(10/14)
BspEI 712 TCCGGA T^CCGGA
BsrBI 519 CCGCTC CCGCTC(-3/-3)
BsrI 415 ACTGG ACTGG(1/-1)
BstEII 289 GGTGACC G^GT[ACGT]ACC
BstPI 289 GGTGACC G^GT[ACGT]ACC
BstYI 181 GGATCC [GA]^GATC[CT]
DraII 71 GGGTCCT [GA]G^G[ACGT]CC[CT]
EagI 80 CGGCCG C^GGCCG
EarI 631 CTCTTC CTCTTC(1/4)
EclXI 80 CGGCCG C^GGCCG
Eco52I 80 CGGCCG C^GGCCG
EcoNI 234 CCTGACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 71 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 289 GGTGACC G^GT[ACGT]ACC
FspI 695 TGCGCA TGC^GCA
Ksp632I 631 CTCTTC CTCTTC(1/4)
MaeI 155 CTAG C^TAG
MaeII 455 ACGT A^CGT
MflI 181 GGATCC [GA]^GATC[CT]
MluNI 296 TGGCCA TGG^CCA
MroI 712 TCCGGA T^CCGGA
MscI 296 TGGCCA TGG^CCA
NcoI 647 CCATGG C^CATGG
NdeI 746 CATATG CA^TATG
NspI 536 GCATGC [GA]CATG^[CT]
PpuMI 71 GGGTCCT [GA]G^G[AT]CC[CT]
SacI 708 GAGCTC GAGCT^C
SmlI 253 CTCAAG C^T[CT][GA]AG
SphI 536 GCATGC GCATG^C
StuI 530 AGGCCT AGG^CCT
TaqI 121 TCGA T^CGA
Tsp509I 229 AATT ^AATT
TthHB8I 121 TCGA T^CGA
XcmI 284 CCACGGGTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 181 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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AfaI 165 GTAC GT^AC
447 GTAC
AspHI 335 GTGCTC G[AT]GC[AT]^C
708 GAGCTC
BpmI 366 CTCCAG CTGGAG(16/14)
705 CTGGAG
BsiEI 80 CGGCCG CG[GA][CT]^CG
84 CGACCG
BsiHKAI 335 GTGCTC G[AT]GC[AT]^C
708 GAGCTC
BsmI 279 GAATGC GAATGC(1/-1)
309 GAATGC
BstF5I 99 CATCC GGATG(2/0)
431 CATCC
CfoI 4 GCGC GCG^C
696 GCGC
DdeI 19 CTGAG C^T[ACGT]AG
524 CTGAG
DsaI 284 CCACGG C^C[GA][CT]GG
647 CCATGG
EaeI 80 CGGCCG [CT]^GGCC[GA]
296 TGGCCA
FauI 14 GCGGG CCCGC(4/6)
604 GCGGG
FokI 99 CATCC GGATG(9/13)
431 CATCC
HhaI 4 GCGC GCG^C
696 GCGC
HinP1I 4 GCGC G^CGC
696 GCGC
HphI 289 GGTGA GGTGA(8/7)
735 GGTGA
RsaI 165 GTAC GT^AC
447 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI AlwI BbvI BcnI
BmyI BsaJI BseRI BsiYI BslI
Bsp1286I BssKI BstNI Cac8I Cfr13I
DpnII EcoRII EcoT14I Fnu4HI HaeIII
HapII HinfI HpaII ItaI MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PvuII Sau3AI Sau96I ScrFI
SfaNI StyI TfiI TseI Tsp45I
TspRI