(Circular) (Six-base) MAPSORT of: pFN21AE6281.seq Check: 2924 from: 1 to: 5461 pFN21AE6281 5461 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3271 278 Size: 53 83 186 936 1735 2468 Fragments arranged by size: 2468 1735 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2658 2804 1937 Size: 721 146 4594 Fragments arranged by size: 4594 721 146 Acc65I G'GTAC_C Cuts at: 2782 2782 Size: 5461 AclI AA'CG_TT Cuts at: 1563 3588 3961 1563 Size: 2025 373 3063 Fragments arranged by size: 3063 2025 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2382 3524 4908 5176 1632 Size: 750 1142 1384 268 1917 Fragments arranged by size: 1917 1384 1142 750 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5264 AflIII A'CryG_T Cuts at: 1203 4360 5337 1203 Size: 3157 977 1327 Fragments arranged by size: 3157 1327 977 AgeI A'CCGG_T Cuts at: 4292 4292 Size: 5461 AhdI GACnn_n'nnGTC Cuts at: 4190 4190 Size: 5461 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5461 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5461 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2088 2120 2088 Size: 32 5429 AlwNI CAG_nnn'CTG Cuts at: 2420 3204 4776 2420 Size: 784 1572 3105 Fragments arranged by size: 3105 1572 784 ApaLI G'TGCA_C Cuts at: 3518 4674 3518 Size: 1156 4305 ApoI r'AATT_y Cuts at: 1493 2770 2996 3148 5257 1493 Size: 1277 226 152 2109 1697 Fragments arranged by size: 2109 1697 1277 226 152 AseI AT'TA_AT Cuts at: 160 1032 4015 160 Size: 872 2983 1606 Fragments arranged by size: 2983 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5461 AvaI C'yCGr_G Cuts at: 1870 1943 2786 1870 Size: 73 843 4545 Fragments arranged by size: 4545 843 73 AvrII C'CTAG_G Cuts at: 2159 2159 Size: 5461 BamHI G'GATC_C Cuts at: 2186 2791 3158 2186 Size: 605 367 4489 Fragments arranged by size: 4489 605 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2782 4237 618 Size: 325 217 627 995 1455 1842 Fragments arranged by size: 1842 1455 995 627 325 217 BanII G_rGCy'C Cuts at: 1391 2717 2780 1391 Size: 1326 63 4072 Fragments arranged by size: 4072 1326 63 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2370 928 Size: 971 471 4019 Fragments arranged by size: 4019 971 471 BbvCI CC'TCA_GC Cuts at: 2024 2024 Size: 5461 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3652 3686 1903 Size: 34 1715 34 3678 Fragments arranged by size: 3678 1715 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3353 4563 3353 Size: 1210 4251 BclI T'GATC_A Cuts at: 1548 1548 Size: 5461 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4072 136 Size: 107 122 71 631 605 2400 1525 Fragments arranged by size: 2400 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5456 5456 Size: 5461 BlpI GC'TnA_GC Cuts at: 2878 2878 Size: 5461 Bme1580I G_kGCm'C Cuts at: 1790 2698 3522 4678 1790 Size: 908 824 1156 2573 Fragments arranged by size: 2573 1156 908 824 BmgBI CAC'GTC Cuts at: 1318 2461 1318 Size: 1143 4318 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2430 3192 4150 Size: 416 53 83 399 24 1013 762 958 Cuts at: 4150 442 Size: 1753 Fragments arranged by size: 1753 1013 958 762 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5461 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2488 2488 Size: 5461 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2356 2731 4121 1239 Size: 1117 375 1390 2579 Fragments arranged by size: 2579 1390 1117 375 Bpu10I CC'TnA_GC Cuts at: 2024 2024 Size: 5461 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2243 3586 4451 4749 2243 Size: 1343 865 298 2955 Fragments arranged by size: 2955 1343 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4124 882 Size: 617 2625 2219 Fragments arranged by size: 2625 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3252 493 Size: 711 2048 2702 Fragments arranged by size: 2702 2048 711 BsaBI GATnn'nnATC Cuts at: 5455 5455 Size: 5461 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3268 3650 4285 275 Size: 53 83 186 936 1735 382 635 1451 Fragments arranged by size: 1735 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2717 3894 4292 4566 4713 5124 2717 Size: 1177 398 274 147 411 3054 Fragments arranged by size: 3054 1177 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5236 Fragments arranged by size: 5236 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2057 2264 2546 1186 Size: 871 207 282 4101 Fragments arranged by size: 4101 871 282 207 BseYI C'CCAG_C Cuts at: 2053 4664 5160 2053 Size: 2611 496 2354 Fragments arranged by size: 2611 2354 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2386 1163 Size: 573 650 4238 Fragments arranged by size: 4238 650 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2088 2092 2911 3672 4700 1540 Size: 275 186 87 4 819 761 1028 2301 Fragments arranged by size: 2301 1028 819 761 275 186 87 4 BsiHKAI G_wGCw'C Cuts at: 2344 2717 3522 3607 4678 2344 Size: 373 805 85 1071 3127 Fragments arranged by size: 3127 1071 805 373 85 BsmI GAATG_Cn' Cuts at: 2290 2320 2972 3065 2290 Size: 30 652 93 4686 Fragments arranged by size: 4686 652 93 30 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2344 2698 2717 2780 3522 3607 4678 Size: 399 554 354 19 63 742 85 1071 Cuts at: 4678 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 554 399 354 85 63 19 BspEI T'CCGG_A Cuts at: 2717 5124 2717 Size: 2407 3054 BspHI T'CATG_A Cuts at: 3350 5080 3350 Size: 1730 3731 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2816 844 Size: 1972 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2526 3348 1394 Size: 429 703 822 3507 Fragments arranged by size: 3507 822 703 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3956 4130 65 Size: 1183 2708 174 1396 Fragments arranged by size: 2708 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4105 4292 1700 Size: 110 2295 187 2869 Fragments arranged by size: 2869 2295 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5461 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5461 BssSI C'ACGA_G Cuts at: 3521 4533 3521 Size: 1012 4449 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5461 BstBI TT'CG_AA Cuts at: 4265 4265 Size: 5461 BstEII G'GTnAC_C Cuts at: 1184 2294 1184 Size: 1110 4351 BstYI r'GATC_y Cuts at: 1972 2186 2791 3158 3543 3560 5012 5098 5110 Size: 214 605 367 385 17 1452 86 12 Cuts at: 5110 5456 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 605 385 367 346 214 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2289 2652 513 Size: 552 939 285 363 3322 Fragments arranged by size: 3322 939 552 363 285 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4722 Fragments arranged by size: 4722 683 56 BtsI GCAGTG_nn' Cuts at: 2977 3771 3791 2977 Size: 794 20 4647 Fragments arranged by size: 4647 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5461 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5426 DraI TTT'AAA Cuts at: 2765 2765 Size: 5461 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4468 809 Size: 507 3152 1802 Fragments arranged by size: 3152 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2085 2301 2908 Size: 54 1425 50 275 18 255 216 607 Cuts at: 2908 3797 5426 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 607 275 255 216 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2085 2908 1537 Size: 275 273 823 4090 Fragments arranged by size: 4090 823 275 273 EarI CTCTTCn'nnn_ Cuts at: 1353 2642 3391 1353 Size: 1289 749 3423 Fragments arranged by size: 3423 1289 749 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2044 2546 4048 4422 4568 387 Size: 1064 138 455 502 1502 374 146 1280 Fragments arranged by size: 1502 1280 1064 502 455 374 146 138 EcoICRI GAG'CTC Cuts at: 2715 2715 Size: 5461 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2356 2382 2731 3524 4121 4908 5176 Size: 393 724 26 349 793 597 787 268 Cuts at: 5176 1239 Size: 1524 Fragments arranged by size: 1524 793 787 724 597 393 349 268 26 EcoNI CCTnn'n_nnAGG Cuts at: 2243 2243 Size: 5461 EcoO109I rG'GnC_Cy Cuts at: 2077 2077 Size: 5461 EcoRI G'AATT_C Cuts at: 2770 3148 2770 Size: 378 5083 FspI TGC'GCA Cuts at: 2702 3967 2702 Size: 1265 4196 HaeII r_GCGC'y Cuts at: 1678 4608 1678 Size: 2930 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2049 2081 4102 4134 4176 Size: 32 135 32 477 32 2021 32 42 Cuts at: 4176 4208 1373 Size: 32 2626 Fragments arranged by size: 2626 2021 477 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2805 3051 669 Size: 988 281 867 246 3079 Fragments arranged by size: 3079 988 867 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5461 HpaI GTT'AAC Cuts at: 3051 3051 Size: 5461 KpnI G_GTAC'C Cuts at: 2786 2786 Size: 5461 MfeI C'AATT_G Cuts at: 3060 3060 Size: 5461 MluI A'CGCG_T Cuts at: 5337 5337 Size: 5461 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4575 4759 1305 Size: 3270 184 2007 Fragments arranged by size: 3270 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2303 10 Size: 54 1425 343 471 3168 Fragments arranged by size: 3168 1425 471 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3419 3778 3937 518 Size: 648 2253 359 159 2042 Fragments arranged by size: 2253 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2014 2197 2321 2550 2737 2877 3554 4702 Size: 408 183 124 229 187 140 677 1148 Cuts at: 4702 4947 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 408 245 229 187 183 140 124 NcoI C'CATG_G Cuts at: 513 1065 2004 2652 513 Size: 552 939 648 3322 Fragments arranged by size: 3322 939 648 552 NdeI CA'TA_TG Cuts at: 387 2752 387 Size: 2365 3096 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5461 NotI GC'GGCC_GC Cuts at: 2908 2908 Size: 5461 NspI r_CATG'y Cuts at: 1132 1229 2545 2819 4364 1132 Size: 97 1316 274 1545 2229 Fragments arranged by size: 2229 1545 1316 274 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5461 PciI A'CATG_T Cuts at: 4360 4360 Size: 5461 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5461 PmeI GTTT'AAAC Cuts at: 2765 2765 Size: 5461 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3530 3562 5043 5075 3530 Size: 32 1481 32 3916 Fragments arranged by size: 3916 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2077 2077 Size: 5461 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5461 PsiI TTA'TAA Cuts at: 3031 3031 Size: 5461 PstI C_TGCA'G Cuts at: 830 2813 830 Size: 1983 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5461 PvuII CAG'CTG Cuts at: 2014 2197 2737 2014 Size: 183 540 4738 Fragments arranged by size: 4738 540 183 SacI G_AGCT'C Cuts at: 2717 2717 Size: 5461 SalI G'TCGA_C Cuts at: 1936 2803 1936 Size: 867 4594 SbfI CC_TGCA'GG Cuts at: 2813 2813 Size: 5461 ScaI AGT'ACT Cuts at: 3709 5421 3709 Size: 1712 3749 SfcI C'TryA_G Cuts at: 826 1045 2809 3944 4625 4816 826 Size: 219 1764 1135 681 191 1471 Fragments arranged by size: 1764 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5461 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5461 SmaI CCC'GGG Cuts at: 1872 2788 1872 Size: 916 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2258 3565 4466 4728 820 Size: 197 926 315 1307 901 262 1553 Fragments arranged by size: 1553 1307 926 901 315 262 197 SnaBI TAC'GTA Cuts at: 493 3252 493 Size: 2759 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5461 SphI G_CATG'C Cuts at: 1132 2545 2819 1132 Size: 1413 274 3774 Fragments arranged by size: 3774 1413 274 SspI AAT'ATT Cuts at: 5 52 3385 5 Size: 47 3333 2081 Fragments arranged by size: 3333 2081 47 StuI AGG'CCT Cuts at: 1517 2537 1517 Size: 1020 4441 StyI C'CwwG_G Cuts at: 513 1065 2004 2159 2244 2584 2652 2900 513 Size: 552 939 155 85 340 68 248 3074 Fragments arranged by size: 3074 939 552 340 248 155 85 68 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2106 3488 3505 3658 3843 4288 325 Size: 1255 526 1382 17 153 185 445 1498 Fragments arranged by size: 1498 1382 1255 526 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3707 5419 96 Size: 274 80 33 51 1443 1730 1712 138 Fragments arranged by size: 1730 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5421 5453 5421 Size: 32 5429 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5461 XbaI T'CTAG_A Cuts at: 2797 2797 Size: 5461 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2296 2296 Size: 5461 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5461 XmaI C'CCGG_G Cuts at: 1870 2786 1870 Size: 916 4545 XmnI GAAnn'nnTTC Cuts at: 3590 3590 Size: 5461 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3269 276 Size: 53 83 186 936 1735 2468 Fragments arranged by size: 2468 1735 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI