(Linear) (Six-base) MAPSORT of: KIEE6281.seq Check: 3191 from: 1 to: 756 KIEE6281.orf With 182 enzymes: SgfI * March 16, 2015 11:29 .. AccI GT'mk_AC Cuts at: 0 653 756 Size: 653 103 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 377 756 Size: 377 379 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 83 115 756 Size: 83 32 641 Fragments arranged by size: 641 83 32 AlwNI CAG_nnn'CTG Cuts at: 0 415 756 Size: 415 341 AvrII C'CTAG_G Cuts at: 0 154 756 Size: 154 602 BamHI G'GATC_C Cuts at: 0 181 756 Size: 181 575 BanII G_rGCy'C Cuts at: 0 712 756 Size: 712 44 BbsI GAAGACnn'nnnn_ Cuts at: 0 365 756 Size: 365 391 BbvCI CC'TCA_GC Cuts at: 0 19 756 Size: 19 737 Bme1580I G_kGCm'C Cuts at: 0 693 756 Size: 693 63 BmgBI CAC'GTC Cuts at: 0 456 756 Size: 456 300 BmrI ACTGGGnnnn_n' Cuts at: 0 425 756 Size: 425 331 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 483 756 Size: 483 273 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 351 726 756 Size: 351 375 30 Fragments arranged by size: 375 351 30 Bpu10I CC'TnA_GC Cuts at: 0 19 756 Size: 19 737 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 238 756 Size: 238 518 BsaWI w'CCGG_w Cuts at: 0 712 756 Size: 712 44 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 52 259 541 756 Size: 52 207 282 215 Fragments arranged by size: 282 215 207 52 BseYI C'CCAG_C Cuts at: 0 48 756 Size: 48 708 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 381 756 Size: 381 375 BsiEI CG_ry'CG Cuts at: 0 83 87 756 Size: 83 4 669 Fragments arranged by size: 669 83 4 BsiHKAI G_wGCw'C Cuts at: 0 339 712 756 Size: 339 373 44 Fragments arranged by size: 373 339 44 BsmI GAATG_Cn' Cuts at: 0 285 315 756 Size: 285 30 441 Fragments arranged by size: 441 285 30 Bsp1286I G_dGCh'C Cuts at: 0 339 693 712 756 Size: 339 354 19 44 Fragments arranged by size: 354 339 44 19 BspEI T'CCGG_A Cuts at: 0 712 756 Size: 712 44 BsrBI CCG'CTC Cuts at: 0 521 756 Size: 521 235 BstEII G'GTnAC_C Cuts at: 0 289 756 Size: 289 467 BstYI r'GATC_y Cuts at: 0 181 756 Size: 181 575 BtgI C'CryG_G Cuts at: 0 284 647 756 Size: 284 363 109 Fragments arranged by size: 363 284 109 EaeI y'GGCC_r Cuts at: 0 80 296 756 Size: 80 216 460 Fragments arranged by size: 460 216 80 EagI C'GGCC_G Cuts at: 0 80 756 Size: 80 676 EarI CTCTTCn'nnn_ Cuts at: 0 637 756 Size: 637 119 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 39 541 756 Size: 39 502 215 Fragments arranged by size: 502 215 39 EcoICRI GAG'CTC Cuts at: 0 710 756 Size: 710 46 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 351 377 726 756 Size: 351 26 349 30 Fragments arranged by size: 351 349 30 26 EcoNI CCTnn'n_nnAGG Cuts at: 0 238 756 Size: 238 518 EcoO109I rG'GnC_Cy Cuts at: 0 72 756 Size: 72 684 FspI TGC'GCA Cuts at: 0 697 756 Size: 697 59 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 44 76 756 Size: 44 32 680 Fragments arranged by size: 680 44 32 MscI TGG'CCA Cuts at: 0 298 756 Size: 298 458 MspA1I CmG'CkG Cuts at: 0 9 192 316 545 732 756 Size: 9 183 124 229 187 24 Fragments arranged by size: 229 187 183 124 24 9 NcoI C'CATG_G Cuts at: 0 647 756 Size: 647 109 NdeI CA'TA_TG Cuts at: 0 747 756 Size: 747 9 NspI r_CATG'y Cuts at: 0 540 756 Size: 540 216 PpuMI rG'GwC_Cy Cuts at: 0 72 756 Size: 72 684 PvuII CAG'CTG Cuts at: 0 9 192 732 756 Size: 9 183 540 24 Fragments arranged by size: 540 183 24 9 SacI G_AGCT'C Cuts at: 0 712 756 Size: 712 44 SmlI C'TyrA_G Cuts at: 0 253 756 Size: 253 503 SphI G_CATG'C Cuts at: 0 540 756 Size: 540 216 StuI AGG'CCT Cuts at: 0 532 756 Size: 532 224 StyI C'CwwG_G Cuts at: 0 154 239 579 647 756 Size: 154 85 340 68 109 Fragments arranged by size: 340 154 109 85 68 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 101 756 Size: 101 655 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 291 756 Size: 291 465 Enzymes that do cut: AccI AcuI AloI AlwNI AvrII BamHI BanII BbsI BbvCI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BsrBI BstEII BstYI BtgI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI Hin4I MscI MspA1I NcoI NdeI NspI PpuMI PvuII SacI SmlI SphI StuI StyI TaqII XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BaeI BanI BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI BmtI BsaI BsaAI BsaBI BsaHI BsaXI BsiWI BsmBI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FalI FseI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MslI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI