(Circular) (Six-base) MAPSORT of: pFN21AE2510.seq Check: 1485 from: 1 to: 8083 pFN21AE2510 8083 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2251 3724 3979 4240 4303 4526 5071 2251 Size: 1473 255 261 63 223 545 5263 Fragments arranged by size: 5263 1473 545 261 255 223 63 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3528 5893 278 Size: 53 83 186 936 1992 2365 2468 Fragments arranged by size: 2468 2365 1992 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2594 5426 1937 Size: 657 2832 4594 Fragments arranged by size: 4594 2832 657 Acc65I G'GTAC_C Cuts at: 5404 5404 Size: 8083 AclI AA'CG_TT Cuts at: 1563 2172 6210 6583 1563 Size: 609 4038 373 3063 Fragments arranged by size: 4038 3063 609 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2786 3852 6146 7530 7798 1632 Size: 1154 1066 2294 1384 268 1917 Fragments arranged by size: 2294 1917 1384 1154 1066 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7886 AflIII A'CryG_T Cuts at: 1203 6982 7959 1203 Size: 5779 977 1327 Fragments arranged by size: 5779 1327 977 AgeI A'CCGG_T Cuts at: 2337 6914 2337 Size: 4577 3506 AhdI GACnn_n'nnGTC Cuts at: 6812 6812 Size: 8083 AleI CACnn'nnGTG Cuts at: 1166 3518 4398 1166 Size: 2352 880 4851 Fragments arranged by size: 4851 2352 880 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2897 3592 1184 Size: 1713 695 5675 Fragments arranged by size: 5675 1713 695 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3515 3547 3515 Size: 32 8051 AlwNI CAG_nnn'CTG Cuts at: 2197 2416 2440 2908 2998 3251 3298 3774 5826 Size: 219 24 468 90 253 47 476 2052 Cuts at: 5826 7398 2197 Size: 1572 2882 Fragments arranged by size: 2882 2052 1572 476 468 253 219 90 47 24 ApaI G_GGCC'C Cuts at: 2082 2223 3806 4118 4691 4745 5049 5204 2082 Size: 141 1583 312 573 54 304 155 4961 Fragments arranged by size: 4961 1583 573 312 304 155 141 54 ApaLI G'TGCA_C Cuts at: 3278 6140 7296 3278 Size: 2862 1156 4065 Fragments arranged by size: 4065 2862 1156 ApoI r'AATT_y Cuts at: 1493 5392 5618 5770 7879 1493 Size: 3899 226 152 2109 1697 Fragments arranged by size: 3899 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6637 160 Size: 872 5605 1606 Fragments arranged by size: 5605 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8083 AvaI C'yCGr_G Cuts at: 1870 1943 2121 2390 4396 4411 4655 5408 1870 Size: 73 178 269 2006 15 244 753 4545 Fragments arranged by size: 4545 2006 753 269 244 178 73 15 BamHI G'GATC_C Cuts at: 3111 5413 5780 3111 Size: 2302 367 5414 Fragments arranged by size: 5414 2302 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2396 2419 2605 3339 3409 Size: 325 217 627 609 23 186 734 70 Cuts at: 3409 3845 4181 4286 4400 5001 5155 5185 5404 Size: 436 336 105 114 601 154 30 219 Cuts at: 5404 6859 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 734 627 609 601 436 336 325 219 217 186 154 114 105 70 30 23 BanII G_rGCy'C Cuts at: 1391 2051 2082 2223 3690 3806 4111 4118 4246 Size: 660 31 141 1467 116 305 7 128 Cuts at: 4246 4350 4691 4736 4745 5022 5049 5204 5265 Size: 104 341 45 9 277 27 155 61 Cuts at: 5265 5402 1391 Size: 137 4072 Fragments arranged by size: 4072 1467 660 341 305 277 155 141 137 128 116 104 61 45 31 27 9 7 BbeI G_GCGC'C Cuts at: 2609 5005 2609 Size: 2396 5687 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3060 3785 928 Size: 971 1161 725 5226 Fragments arranged by size: 5226 1161 971 725 BbvCI CC'TCA_GC Cuts at: 2830 2966 3335 3976 4460 2830 Size: 136 369 641 484 6453 Fragments arranged by size: 6453 641 484 369 136 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3184 3218 4979 5013 5361 5395 6274 Size: 34 1247 34 1761 34 348 34 879 Cuts at: 6274 6308 1903 Size: 34 3678 Fragments arranged by size: 3678 1761 1247 879 348 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5975 7185 5975 Size: 1210 6873 BclI T'GATC_A Cuts at: 1548 2583 1548 Size: 1035 7048 BfrBI ATG'CAT Cuts at: 3382 3382 Size: 8083 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2034 3353 Size: 107 122 71 631 605 337 25 1319 Cuts at: 3353 3941 6694 136 Size: 588 2753 1525 Fragments arranged by size: 2753 1525 1319 631 605 588 337 122 107 71 25 BglII A'GATC_T Cuts at: 3899 8078 3899 Size: 4179 3904 BlpI GC'TnA_GC Cuts at: 4236 4245 4928 5500 4236 Size: 9 683 572 6819 Fragments arranged by size: 6819 683 572 9 Bme1580I G_kGCm'C Cuts at: 1790 2082 2223 2991 3282 3412 3806 3848 3856 Size: 292 141 768 291 130 394 42 8 Cuts at: 3856 4118 4186 4289 4525 4691 4745 5049 5158 Size: 262 68 103 236 166 54 304 109 Cuts at: 5158 5204 6144 7300 1790 Size: 46 940 1156 2573 Fragments arranged by size: 2573 1156 940 768 394 304 292 291 262 236 166 141 130 109 103 68 54 46 42 8 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8083 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2076 2715 3801 Size: 416 53 83 399 24 659 639 1086 Cuts at: 3801 3803 4567 4701 4715 5120 5814 6772 442 Size: 2 764 134 14 405 694 958 1753 Fragments arranged by size: 1753 1086 958 764 694 659 639 416 405 399 134 83 53 24 14 2 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8083 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4752 4752 Size: 8083 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2190 2253 2462 3344 3458 3659 4352 4747 Size: 951 63 209 882 114 201 693 395 Cuts at: 4747 4852 6743 1239 Size: 105 1891 2579 Fragments arranged by size: 2579 1891 951 882 693 395 209 201 114 105 63 Bpu10I CC'TnA_GC Cuts at: 2830 2966 3335 3976 4460 4529 5016 5208 2830 Size: 136 369 641 484 69 487 192 5705 Fragments arranged by size: 5705 641 487 484 369 192 136 69 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4471 6208 7073 7371 4471 Size: 1737 865 298 5183 Fragments arranged by size: 5183 1737 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6746 882 Size: 617 5247 2219 Fragments arranged by size: 5247 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5874 493 Size: 711 4670 2702 Fragments arranged by size: 4670 2702 711 BsaBI GATnn'nnATC Cuts at: 8077 8077 Size: 8083 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2546 2606 3525 3677 Size: 53 83 186 936 1013 60 919 152 Cuts at: 3677 4071 5002 5890 6272 6907 275 Size: 394 931 888 382 635 1451 Fragments arranged by size: 1451 1013 936 931 919 888 635 394 382 186 152 83 60 53 BsaWI w'CCGG_w Cuts at: 2295 2337 2352 2371 6516 6914 7188 7335 7746 Size: 42 15 19 4145 398 274 147 411 Cuts at: 7746 2295 Size: 2632 Fragments arranged by size: 4145 2632 411 398 274 147 42 19 15 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2435 2465 2625 2655 3425 Size: 30 165 30 1032 30 160 30 770 Cuts at: 3425 3455 3908 3938 4887 4917 1178 Size: 30 453 30 949 30 4344 Fragments arranged by size: 4344 1032 949 770 453 165 160 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2109 2522 2555 3272 3275 3929 4062 4477 Size: 923 413 33 717 3 654 133 415 Cuts at: 4477 4744 5065 5068 1186 Size: 267 321 3 4201 Fragments arranged by size: 4201 923 717 654 415 413 321 267 133 33 3 3 BseYI C'CCAG_C Cuts at: 2072 2222 3670 3838 3952 3959 4207 4524 4624 Size: 150 1448 168 114 7 248 317 100 Cuts at: 4624 4714 4986 5339 7286 7782 2072 Size: 90 272 353 1947 496 2373 Fragments arranged by size: 2373 1947 1448 496 353 317 272 248 168 150 114 100 90 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2075 2279 3506 4111 4734 1163 Size: 573 339 204 1227 605 623 4512 Fragments arranged by size: 4512 1227 623 605 573 339 204 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2521 2574 4009 5533 6294 7322 Size: 275 186 520 53 1435 1524 761 1028 Cuts at: 7322 1540 Size: 2301 Fragments arranged by size: 2301 1524 1435 1028 761 520 275 186 53 BsiHKAI G_wGCw'C Cuts at: 2493 2964 3282 3970 4736 5265 6144 6229 7300 Size: 471 318 688 766 529 879 85 1071 Cuts at: 7300 2493 Size: 3276 Fragments arranged by size: 3276 1071 879 766 688 529 471 318 85 BsmI GAATG_Cn' Cuts at: 3134 5594 5687 3134 Size: 2460 93 5530 Fragments arranged by size: 5530 2460 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2826 2826 Size: 8083 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2082 2223 2493 2964 2991 3282 Size: 399 261 31 141 270 471 27 291 Cuts at: 3282 3412 3690 3806 3848 3856 3970 4111 4118 Size: 130 278 116 42 8 114 141 7 Cuts at: 4118 4186 4246 4289 4350 4525 4691 4736 4745 Size: 68 60 43 61 175 166 45 9 Cuts at: 4745 5022 5049 5158 5204 5265 5402 6144 6229 Size: 277 27 109 46 61 137 742 85 Cuts at: 6229 7300 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 471 399 291 278 277 270 261 175 166 141 141 137 130 116 114 109 85 68 61 61 60 46 45 43 42 31 27 27 9 8 7 BspEI T'CCGG_A Cuts at: 2295 2371 7746 2295 Size: 76 5375 2632 Fragments arranged by size: 5375 2632 76 BspHI T'CATG_A Cuts at: 5972 7702 5972 Size: 1730 6353 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2251 3339 3426 3724 3979 4240 4303 4526 Size: 1407 1088 87 298 255 261 63 223 Cuts at: 4526 5071 5438 844 Size: 545 367 3489 Fragments arranged by size: 3489 1407 1088 545 367 298 261 255 223 87 63 BsrBI CCG'CTC Cuts at: 1394 1823 2749 4240 4320 4827 5970 1394 Size: 429 926 1491 80 507 1143 3507 Fragments arranged by size: 3507 1491 1143 926 507 429 80 BsrDI GCAATG_nn' Cuts at: 65 1248 6578 6752 65 Size: 1183 5330 174 1396 Fragments arranged by size: 5330 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2287 2337 2520 2861 3051 3554 3666 Size: 110 477 50 183 341 190 503 112 Cuts at: 3666 6727 6914 1700 Size: 3061 187 2869 Fragments arranged by size: 3061 2869 503 477 341 190 187 183 112 110 50 BsrGI T'GTAC_A Cuts at: 96 5280 96 Size: 5184 2899 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8083 BssSI C'ACGA_G Cuts at: 2847 3092 6143 7155 2847 Size: 245 3051 1012 3775 Fragments arranged by size: 3775 3051 1012 245 BstAPI GCAn_nnn'nTGC Cuts at: 794 2908 794 Size: 2114 5969 BstBI TT'CG_AA Cuts at: 6887 6887 Size: 8083 BstEII G'GTnAC_C Cuts at: 1184 2263 1184 Size: 1079 7004 BstXI CCAn_nnnn'nTGG Cuts at: 3221 3436 3221 Size: 215 7868 BstYI r'GATC_y Cuts at: 1972 3045 3111 3899 5113 5413 5780 6165 6182 Size: 1073 66 788 1214 300 367 385 17 Cuts at: 6182 7634 7720 7732 8078 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1214 1073 788 385 367 346 300 86 66 17 12 BstZ17I GTA'TAC Cuts at: 2595 2595 Size: 8083 Bsu36I CC'TnA_GG Cuts at: 4360 4360 Size: 8083 BtgI C'CryG_G Cuts at: 513 1065 2004 2135 2840 3028 3429 3681 4476 Size: 552 939 131 705 188 401 252 795 Cuts at: 4476 513 Size: 4120 Fragments arranged by size: 4120 939 795 705 552 401 252 188 131 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3912 487 Size: 683 56 2686 4658 Fragments arranged by size: 4658 2686 683 56 BtsI GCAGTG_nn' Cuts at: 2067 2423 2571 4142 5599 6393 6413 2067 Size: 356 148 1571 1457 794 20 3737 Fragments arranged by size: 3737 1571 1457 794 356 148 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8083 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8048 DraI TTT'AAA Cuts at: 5387 5387 Size: 8083 DraIII CAC_nnn'GTG Cuts at: 3034 3157 3034 Size: 123 7960 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7090 809 Size: 507 5774 1802 Fragments arranged by size: 5774 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2182 2285 2518 Size: 54 1425 50 275 18 352 103 233 Cuts at: 2518 2571 2750 2859 3396 4006 4254 4888 5530 Size: 53 179 109 537 610 248 634 642 Cuts at: 5530 6419 8048 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 642 634 610 537 352 275 248 233 179 109 103 54 53 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2518 2571 4006 5530 1537 Size: 275 706 53 1435 1524 4090 Fragments arranged by size: 4090 1524 1435 706 275 53 EarI CTCTTCn'nnn_ Cuts at: 1353 2099 2744 5074 5086 6013 1353 Size: 746 645 2330 12 927 3423 Fragments arranged by size: 3423 2330 927 746 645 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2786 3541 6670 7044 7190 387 Size: 1064 138 1197 755 3129 374 146 1280 Fragments arranged by size: 3129 1280 1197 1064 755 374 146 138 EcoICRI GAG'CTC Cuts at: 4734 5263 4734 Size: 529 7554 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2190 2253 2462 2786 3344 3458 3659 Size: 393 558 63 209 324 558 114 201 Cuts at: 3659 3852 4352 4747 4852 6146 6743 7530 7798 Size: 193 500 395 105 1294 597 787 268 Cuts at: 7798 1239 Size: 1524 Fragments arranged by size: 1524 1294 787 597 558 558 500 395 393 324 268 209 201 193 114 105 63 EcoNI CCTnn'n_nnAGG Cuts at: 2537 4489 5248 2537 Size: 1952 759 5372 Fragments arranged by size: 5372 1952 759 EcoO109I rG'GnC_Cy Cuts at: 2078 2126 2219 3318 3703 3802 3803 4057 4114 Size: 48 93 1099 385 99 1 254 57 Cuts at: 4114 4356 4408 4415 4482 4505 4660 4688 4742 Size: 242 52 7 67 23 155 28 54 Cuts at: 4742 4803 4911 5045 5054 5165 5192 5200 5201 Size: 61 108 134 9 111 27 8 1 Cuts at: 5201 5274 2078 Size: 73 4887 Fragments arranged by size: 4887 1099 385 254 242 155 134 111 108 99 93 73 67 61 57 54 52 48 28 27 23 9 8 7 1 1 EcoRI G'AATT_C Cuts at: 5392 5770 5392 Size: 378 7705 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2716 3196 3802 4690 2716 Size: 480 606 888 6109 Fragments arranged by size: 6109 888 606 480 FseI GG_CCGG'CC Cuts at: 2865 2865 Size: 8083 FspI TGC'GCA Cuts at: 6589 6589 Size: 8083 HaeII r_GCGC'y Cuts at: 1678 2609 5005 7230 1678 Size: 931 2396 2225 2531 Fragments arranged by size: 2531 2396 2225 931 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2435 2467 2517 2531 2549 Size: 32 135 32 863 32 50 14 18 Cuts at: 2549 2563 2603 2625 2627 2635 2657 2659 3227 Size: 14 40 22 2 8 22 2 568 Cuts at: 3227 3259 3425 3457 3908 3940 4121 4153 6724 Size: 32 166 32 451 32 181 32 2571 Cuts at: 6724 6756 6798 6830 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2571 863 568 451 181 166 135 50 42 40 32 32 32 32 32 32 32 32 32 22 22 18 14 14 8 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 2176 5032 5427 5673 669 Size: 988 281 238 2856 395 246 3079 Fragments arranged by size: 3079 2856 988 395 281 246 238 HindIII A'AGCT_T Cuts at: 748 748 Size: 8083 HpaI GTT'AAC Cuts at: 5673 5673 Size: 8083 KasI G'GCGC_C Cuts at: 2605 5001 2605 Size: 2396 5687 KpnI G_GTAC'C Cuts at: 5408 5408 Size: 8083 MfeI C'AATT_G Cuts at: 5682 5682 Size: 8083 MluI A'CGCG_T Cuts at: 7959 7959 Size: 8083 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3096 7197 7381 1305 Size: 1791 4101 184 2007 Fragments arranged by size: 4101 2007 1791 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2184 3398 4890 10 Size: 54 1425 343 352 1214 1492 3203 Fragments arranged by size: 3203 1492 1425 1214 352 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2498 2879 3245 3377 3518 4398 6041 Size: 648 1332 381 366 132 141 880 1643 Cuts at: 6041 6400 6559 518 Size: 359 159 2042 Fragments arranged by size: 2042 1643 1332 880 648 381 366 359 159 141 132 MspA1I CmG'CkG Cuts at: 1606 2137 2834 2905 2917 3298 3576 3612 3754 Size: 531 697 71 12 381 278 36 142 Cuts at: 3754 3774 3939 4063 4471 5343 5499 6176 7324 Size: 20 165 124 408 872 156 677 1148 Cuts at: 7324 7569 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 872 697 677 531 408 381 278 245 165 156 142 124 71 36 20 12 NaeI GCC'GGC Cuts at: 2522 2863 3556 3668 2522 Size: 341 693 112 6937 Fragments arranged by size: 6937 693 341 112 NarI GG'CG_CC Cuts at: 2606 5002 2606 Size: 2396 5687 NcoI C'CATG_G Cuts at: 513 1065 2004 3429 4476 513 Size: 552 939 1425 1047 4120 Fragments arranged by size: 4120 1425 1047 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8083 NgoMIV G'CCGG_C Cuts at: 2520 2861 3554 3666 2520 Size: 341 693 112 6937 Fragments arranged by size: 6937 693 341 112 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8083 NotI GC'GGCC_GC Cuts at: 2571 5530 2571 Size: 2959 5124 NsiI A_TGCA'T Cuts at: 3384 3384 Size: 8083 NspI r_CATG'y Cuts at: 1132 1229 2659 3382 3386 4885 5441 6986 1132 Size: 97 1430 723 4 1499 556 1545 2229 Fragments arranged by size: 2229 1545 1499 1430 723 556 97 4 PasI CC'CwG_GG Cuts at: 1348 2039 2809 3661 4111 4175 4351 4632 4684 Size: 691 770 852 450 64 176 281 52 Cuts at: 4684 5049 5160 5196 1348 Size: 365 111 36 4235 Fragments arranged by size: 4235 852 770 691 450 365 281 176 111 64 52 36 PciI A'CATG_T Cuts at: 6982 6982 Size: 8083 PflMI CCAn_nnn'nTGG Cuts at: 1108 2416 3034 3405 1108 Size: 1308 618 371 5786 Fragments arranged by size: 5786 1308 618 371 PfoI T'CCnGG_A Cuts at: 3114 3471 4829 3114 Size: 357 1358 6368 Fragments arranged by size: 6368 1358 357 PmeI GTTT'AAAC Cuts at: 5387 5387 Size: 8083 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2234 2266 6152 6184 7665 7697 2234 Size: 32 3886 32 1481 32 2620 Fragments arranged by size: 3886 2620 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3318 4356 4408 4415 4482 4660 4803 4911 5054 Size: 1038 52 7 67 178 143 108 143 Cuts at: 5054 5165 5192 5274 3318 Size: 111 27 82 6127 Fragments arranged by size: 6127 1038 178 143 143 111 108 82 67 52 27 7 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8083 PsiI TTA'TAA Cuts at: 5653 5653 Size: 8083 PspOMI G'GGCC_C Cuts at: 2078 2219 3802 4114 4687 4741 5045 5200 2078 Size: 141 1583 312 573 54 304 155 4961 Fragments arranged by size: 4961 1583 573 312 304 155 141 54 PspXI vC'TCGA_Gb Cuts at: 2121 2121 Size: 8083 PstI C_TGCA'G Cuts at: 830 2436 2566 3144 3571 5435 830 Size: 1606 130 578 427 1864 3478 Fragments arranged by size: 3478 1864 1606 578 427 130 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8083 PvuII CAG'CTG Cuts at: 2834 3298 3612 3774 5343 2834 Size: 464 314 162 1569 5574 Fragments arranged by size: 5574 1569 464 314 162 SacI G_AGCT'C Cuts at: 4736 5265 4736 Size: 529 7554 SacII CC_GC'GG Cuts at: 2138 2138 Size: 8083 SalI G'TCGA_C Cuts at: 1936 5425 1936 Size: 3489 4594 SanDI GG'GwC_CC Cuts at: 4408 4415 4660 4803 5054 5165 5192 4408 Size: 7 245 143 251 111 27 7299 Fragments arranged by size: 7299 251 245 143 111 27 7 SbfI CC_TGCA'GG Cuts at: 3144 3571 5435 3144 Size: 427 1864 5792 Fragments arranged by size: 5792 1864 427 ScaI AGT'ACT Cuts at: 6331 8043 6331 Size: 1712 6371 SfcI C'TryA_G Cuts at: 826 1045 2432 2562 3140 3567 5224 5431 6566 Size: 219 1387 130 578 427 1657 207 1135 Cuts at: 6566 7247 7438 826 Size: 681 191 1471 Fragments arranged by size: 1657 1471 1387 1135 681 578 427 219 207 191 130 SfoI GGC'GCC Cuts at: 2607 5003 2607 Size: 2396 5687 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8083 SgrAI Cr'CCGG_yG Cuts at: 1700 2337 1700 Size: 637 7446 SmaI CCC'GGG Cuts at: 1872 4398 4413 5410 1872 Size: 2526 15 997 4545 Fragments arranged by size: 4545 2526 997 15 SmlI C'TyrA_G Cuts at: 820 1017 1943 2121 4486 6187 7088 7350 820 Size: 197 926 178 2365 1701 901 262 1553 Fragments arranged by size: 2365 1701 1553 926 901 262 197 178 SnaBI TAC'GTA Cuts at: 493 5874 493 Size: 5381 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8083 SphI G_CATG'C Cuts at: 1132 3382 3386 4885 5441 1132 Size: 2250 4 1499 556 3774 Fragments arranged by size: 3774 2250 1499 556 4 SspI AAT'ATT Cuts at: 5 52 6007 5 Size: 47 5955 2081 Fragments arranged by size: 5955 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8083 StyI C'CwwG_G Cuts at: 513 1065 2004 3429 3699 4433 4476 4969 5522 Size: 552 939 1425 270 734 43 493 553 Cuts at: 5522 513 Size: 3074 Fragments arranged by size: 3074 1425 939 734 553 552 493 270 43 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2677 3427 3443 3530 3674 4112 6110 Size: 1255 1097 750 16 87 144 438 1998 Cuts at: 6110 6127 6280 6465 6910 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1998 1498 1255 1097 750 445 438 185 153 144 87 17 16 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4776 5280 6329 Size: 274 80 33 51 1443 2799 504 1049 Cuts at: 6329 8041 96 Size: 1712 138 Fragments arranged by size: 2799 1712 1443 1049 504 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2541 2573 3143 3175 3217 3249 4887 4919 5258 Size: 32 570 32 42 32 1638 32 339 Cuts at: 5258 5290 8043 8075 2541 Size: 32 2753 32 2549 Fragments arranged by size: 2753 2549 1638 570 339 42 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2449 2639 3887 1316 Size: 1133 190 1248 5512 Fragments arranged by size: 5512 1248 1133 190 XbaI T'CTAG_A Cuts at: 5419 5419 Size: 8083 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2728 4277 4722 2728 Size: 1549 445 6089 Fragments arranged by size: 6089 1549 445 XhoI C'TCGA_G Cuts at: 1943 2121 1943 Size: 178 7905 XmaI C'CCGG_G Cuts at: 1870 4396 4411 5408 1870 Size: 2526 15 997 4545 Fragments arranged by size: 4545 2526 997 15 XmnI GAAnn'nnTTC Cuts at: 3782 5107 6212 3782 Size: 1325 1105 5653 Fragments arranged by size: 5653 1325 1105 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3526 5891 276 Size: 53 83 186 936 1992 2365 2468 Fragments arranged by size: 2468 2365 1992 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BsiWI EcoRV FspAI NruI PacI PmlI PsrI RsrII SapI SexAI SfiI SrfI SwaI