################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BsrI BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI Eam1105I EarI Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HindIII HpaI HphI KpnI Ksp632I MaeIII MamI MboII MfeI MflI MluI MluNI MroI MscI MseI MunI NcoI NdeI NheI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SalI SapI SbfI ScaI SfaNI SfcI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I XbaI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 427 GACGTC GACGT^C AviII 338 TGCGCA TGC^GCA BfaI 679 CTAG C^TAG BsgI 313 CTGCAC GTGCAG(16/14) BsrBI 226 GAGCGG CCGCTC(-3/-3) BsrFI 571 GCCGGC [GA]^CCGG[CT] BstF5I 642 CATCC GGATG(2/0) Cfr10I 571 GCCGGC [GA]^CCGG[CT] DpnII 480 GATC ^GATC DraIII 64 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 421 GACTTCGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 328 CGGCCG C^GGCCG EclXI 328 CGGCCG C^GGCCG Eco52I 328 CGGCCG C^GGCCG EcoNI 105 CCTGCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FokI 642 CATCC GGATG(9/13) FspI 338 TGCGCA TGC^GCA HincII 502 GTCAAC GT[CT]^[GA]AC HindII 502 GTCAAC GT[CT]^[GA]AC HinfI 94 GACTC G^A[ACGT]TC KspI 322 CCGCGG CCGC^GG MaeI 679 CTAG C^TAG MboI 480 GATC ^GATC NaeI 571 GCCGGC GCC^GGC NdeII 480 GATC ^GATC NgoMIV 571 GCCGGC G^CCGGC NotI 327 GCGGCCGC GC^GGCCGC NspI 275 ACATGC [GA]CATG^[CT] PaeR7I 406 CTCGAG C^TCGAG PflMI 60 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 94 GACTC GAGTC(4/5) PpuMI 458 AGGTCCT [GA]G^G[AT]CC[CT] SacI 550 GAGCTC GAGCT^C SacII 322 CCGCGG CCGC^GG Sau3AI 480 GATC ^GATC SexAI 65 ACCTGGT A^CC[AT]GGT SmlI 406 CTCGAG C^T[CT][GA]AG Van91I 60 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 623 CCAGGAAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 406 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AspHI 387 GAGCAC G[AT]GC[AT]^C 550 GAGCTC AvaI 246 CTCGGG C^[CT]CG[GA]G 406 CTCGAG BanII 34 GGGCTC G[GA]GC[CT]^C 550 GAGCTC BglI 321 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 529 GCCCGGCAGGC BlpI 384 GCTGAGC GC^T[ACGT]AGC 699 GCTGAGC BpmI 289 CTGGAG CTGGAG(16/14) 448 CTGGAG Bpu1102I 384 GCTGAGC GC^T[ACGT]AGC 699 GCTGAGC BsiHKAI 387 GAGCAC G[AT]GC[AT]^C 550 GAGCTC BsoBI 246 CTCGGG C^[CT]CG[GA]G 406 CTCGAG BstAPI 99 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 111 GCAGGAGCTGC CelII 384 GCTGAGC GC^T[ACGT]AGC 699 GCTGAGC DdeI 385 CTGAG C^T[ACGT]AG 700 CTGAG DsaI 322 CCGCGG C^C[GA][CT]GG 560 CCACGG HgaI 126 GACGC GACGC(5/10) 281 GACGC MaeII 428 ACGT A^CGT 506 ACGT MslI 342 CACAGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 489 CATGGAGATG NlaIII 276 CATG CATG^ 489 CATG ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AccII AciI AcyI AluI AlwNI AvaII BanI BbeI BbvI BcnI BmyI BsaHI BsaJI BseRI BsiEI BsiYI BslI Bsp1286I BspMI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr13I DraII EaeI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI KasI MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIV Sau96I ScrFI SfoI TaqI TseI TthHB8I