(Circular) (Six-base) MAPSORT of: pFN21AE6601.seq Check: 3522 from: 1 to: 5410 pFN21AE6601 5410 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2174 2174 Size: 5410 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2436 3220 278 Size: 53 83 186 936 900 784 2468 Fragments arranged by size: 2468 936 900 784 186 83 53 AccI GT'mk_AC Cuts at: 1937 2753 1937 Size: 816 4594 Acc65I G'GTAC_C Cuts at: 2731 2731 Size: 5410 AclI AA'CG_TT Cuts at: 1563 3537 3910 1563 Size: 1974 373 3063 Fragments arranged by size: 3063 1974 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2453 3473 4857 5125 1632 Size: 821 1020 1384 268 1917 Fragments arranged by size: 1917 1384 1020 821 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5213 AflIII A'CryG_T Cuts at: 1203 4309 5286 1203 Size: 3106 977 1327 Fragments arranged by size: 3106 1327 977 AgeI A'CCGG_T Cuts at: 4241 4241 Size: 5410 AhdI GACnn_n'nnGTC Cuts at: 4139 4139 Size: 5410 AleI CACnn'nnGTG Cuts at: 1166 2351 1166 Size: 1185 4225 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2045 1184 Size: 861 4549 AlwNI CAG_nnn'CTG Cuts at: 2110 2122 2152 3153 4725 2110 Size: 12 30 1001 1572 2795 Fragments arranged by size: 2795 1572 1001 30 12 ApaLI G'TGCA_C Cuts at: 3467 4623 3467 Size: 1156 4254 ApoI r'AATT_y Cuts at: 1493 2719 2945 3097 5206 1493 Size: 1226 226 152 2109 1697 Fragments arranged by size: 2109 1697 1226 226 152 AseI AT'TA_AT Cuts at: 160 1032 3964 160 Size: 872 2932 1606 Fragments arranged by size: 2932 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5410 AvaI C'yCGr_G Cuts at: 1870 1943 2251 2411 2735 1870 Size: 73 308 160 324 4545 Fragments arranged by size: 4545 324 308 160 73 BamHI G'GATC_C Cuts at: 2740 3107 2740 Size: 367 5043 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2303 2360 2549 2657 2731 Size: 325 217 627 516 57 189 108 74 Cuts at: 2731 4186 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 516 325 217 189 108 74 57 BanII G_rGCy'C Cuts at: 1391 2043 2559 2729 1391 Size: 652 516 170 4072 Fragments arranged by size: 4072 652 516 170 BbeI G_GCGC'C Cuts at: 2307 2364 2553 2661 2307 Size: 57 189 108 5056 Fragments arranged by size: 5056 189 108 57 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4439 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3601 3635 1903 Size: 34 1664 34 3678 Fragments arranged by size: 3678 1664 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3302 4512 3302 Size: 1210 4200 BclI T'GATC_A Cuts at: 1548 1548 Size: 5410 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2332 2540 4021 Size: 107 122 71 631 605 660 208 1481 Cuts at: 4021 136 Size: 1525 Fragments arranged by size: 1525 1481 660 631 605 208 122 107 71 BglII A'GATC_T Cuts at: 5405 5405 Size: 5410 BlpI GC'TnA_GC Cuts at: 2390 2705 2827 2390 Size: 315 122 4973 Fragments arranged by size: 4973 315 122 Bme1580I G_kGCm'C Cuts at: 1790 2517 3471 4627 1790 Size: 727 954 1156 2573 Fragments arranged by size: 2573 1156 954 727 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5410 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3141 4099 442 Size: 416 53 83 399 24 1724 958 1753 Fragments arranged by size: 1753 1724 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5410 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2315 2474 4070 1239 Size: 1076 159 1596 2579 Fragments arranged by size: 2579 1596 1076 159 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3535 4400 4698 3535 Size: 865 298 4247 Fragments arranged by size: 4247 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4073 882 Size: 617 2574 2219 Fragments arranged by size: 2574 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3201 493 Size: 711 1997 2702 Fragments arranged by size: 2702 1997 711 BsaBI GATnn'nnATC Cuts at: 5404 5404 Size: 5410 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2304 2361 2433 2550 Size: 53 83 186 936 771 57 72 117 Cuts at: 2550 2658 3217 3599 4234 275 Size: 108 559 382 635 1451 Fragments arranged by size: 1451 936 771 635 559 382 186 117 108 83 72 57 53 BsaWI w'CCGG_w Cuts at: 3843 4241 4515 4662 5073 3843 Size: 398 274 147 411 4180 Fragments arranged by size: 4180 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5185 Fragments arranged by size: 5185 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 2356 2575 1186 Size: 844 326 219 4021 Fragments arranged by size: 4021 844 326 219 BseYI C'CCAG_C Cuts at: 2299 4613 5109 2299 Size: 2314 496 2600 Fragments arranged by size: 2600 2314 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2303 1163 Size: 573 567 4270 Fragments arranged by size: 4270 573 567 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2197 2336 2592 2860 3621 4649 Size: 275 186 196 139 256 268 761 1028 Cuts at: 4649 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 275 268 256 196 186 139 BsiHKAI G_wGCw'C Cuts at: 2396 2559 3471 3556 4627 2396 Size: 163 912 85 1071 3179 Fragments arranged by size: 3179 1071 912 163 85 BsmI GAATG_Cn' Cuts at: 2921 3014 2921 Size: 93 5317 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2396 2517 2559 2729 3471 3556 Size: 399 253 353 121 42 170 742 85 Cuts at: 3556 4627 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 353 253 170 121 85 42 BspEI T'CCGG_A Cuts at: 5073 5073 Size: 5410 BspHI T'CATG_A Cuts at: 3299 5029 3299 Size: 1730 3680 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2118 2174 2274 2451 2765 844 Size: 1274 56 100 177 314 3489 Fragments arranged by size: 3489 1274 314 177 100 56 BsrBI CCG'CTC Cuts at: 1394 1823 2233 3297 1394 Size: 429 410 1064 3507 Fragments arranged by size: 3507 1064 429 410 BsrDI GCAATG_nn' Cuts at: 65 1248 3905 4079 65 Size: 1183 2657 174 1396 Fragments arranged by size: 2657 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2576 4054 4241 1700 Size: 110 766 1478 187 2869 Fragments arranged by size: 2869 1478 766 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5410 BssHII G'CGCG_C Cuts at: 1925 2218 2289 2381 2383 2385 1925 Size: 293 71 92 2 2 4950 Fragments arranged by size: 4950 293 92 71 2 2 BssSI C'ACGA_G Cuts at: 3470 4482 3470 Size: 1012 4398 BstAPI GCAn_nnn'nTGC Cuts at: 794 2110 2122 794 Size: 1316 12 4082 Fragments arranged by size: 4082 1316 12 BstBI TT'CG_AA Cuts at: 4214 4214 Size: 5410 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5410 BstYI r'GATC_y Cuts at: 1972 2740 3107 3492 3509 4961 5047 5059 5405 Size: 768 367 385 17 1452 86 12 346 Cuts at: 5405 1972 Size: 1977 Fragments arranged by size: 1977 1452 768 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2327 2565 513 Size: 552 939 323 238 3358 Fragments arranged by size: 3358 939 552 323 238 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4671 Fragments arranged by size: 4671 683 56 BtsI GCAGTG_nn' Cuts at: 2926 3720 3740 2926 Size: 794 20 4596 Fragments arranged by size: 4596 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5410 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5375 DraI TTT'AAA Cuts at: 2714 2714 Size: 5410 DraIII CAC_nnn'GTG Cuts at: 2074 2074 Size: 5410 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2432 4417 809 Size: 507 1116 1985 1802 Fragments arranged by size: 1985 1802 1116 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2205 2333 2640 Size: 54 1425 50 275 18 375 128 307 Cuts at: 2640 2857 3746 5375 8 Size: 217 889 1629 43 Fragments arranged by size: 1629 1425 889 375 307 275 217 128 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2333 2857 1537 Size: 275 521 524 4090 Fragments arranged by size: 4090 524 521 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3340 1353 Size: 1987 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2111 2432 3997 4371 4517 387 Size: 1064 138 522 321 1565 374 146 1280 Fragments arranged by size: 1565 1280 1064 522 374 321 146 138 EcoICRI GAG'CTC Cuts at: 2557 2557 Size: 5410 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2315 2453 2474 3473 4070 4857 5125 Size: 393 683 138 21 999 597 787 268 Cuts at: 5125 1239 Size: 1524 Fragments arranged by size: 1524 999 787 683 597 393 268 138 21 EcoNI CCTnn'n_nnAGG Cuts at: 2114 2114 Size: 5410 EcoO109I rG'GnC_Cy Cuts at: 2200 2464 2635 2200 Size: 264 171 4975 Fragments arranged by size: 4975 264 171 EcoRI G'AATT_C Cuts at: 2719 3097 2719 Size: 378 5032 FspI TGC'GCA Cuts at: 2345 3916 2345 Size: 1571 3839 FspAI rTGC'GCAy Cuts at: 2345 2345 Size: 5410 HaeII r_GCGC'y Cuts at: 1678 2030 2307 2364 2553 2577 2661 4557 1678 Size: 352 277 57 189 24 84 1896 2531 Fragments arranged by size: 2531 1896 352 277 189 84 57 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2546 2578 4051 4083 4125 Size: 32 135 32 974 32 1473 32 42 Cuts at: 4125 4157 1373 Size: 32 2626 Fragments arranged by size: 2626 1473 974 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2509 2754 3000 669 Size: 988 281 571 245 246 3079 Fragments arranged by size: 3079 988 571 281 246 245 HindIII A'AGCT_T Cuts at: 748 748 Size: 5410 HpaI GTT'AAC Cuts at: 3000 3000 Size: 5410 KasI G'GCGC_C Cuts at: 2303 2360 2549 2657 2303 Size: 57 189 108 5056 Fragments arranged by size: 5056 189 108 57 KpnI G_GTAC'C Cuts at: 2735 2735 Size: 5410 MfeI C'AATT_G Cuts at: 3009 3009 Size: 5410 MluI A'CGCG_T Cuts at: 5286 5286 Size: 5410 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4524 4708 1305 Size: 3219 184 2007 Fragments arranged by size: 3219 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3588 Fragments arranged by size: 3588 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2351 2498 3368 3727 3886 518 Size: 648 1185 147 870 359 159 2042 Fragments arranged by size: 2042 1185 870 648 359 159 147 MspA1I CmG'CkG Cuts at: 1606 2317 2329 2338 2520 2826 3503 4651 4896 Size: 711 12 9 182 306 677 1148 245 Cuts at: 4896 1606 Size: 2120 Fragments arranged by size: 2120 1148 711 677 306 245 182 12 9 NaeI GCC'GGC Cuts at: 2578 2578 Size: 5410 NarI GG'CG_CC Cuts at: 2304 2361 2550 2658 2304 Size: 57 189 108 5056 Fragments arranged by size: 5056 189 108 57 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3919 Fragments arranged by size: 3919 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5410 NgoMIV G'CCGG_C Cuts at: 2576 2576 Size: 5410 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5410 NotI GC'GGCC_GC Cuts at: 2333 2857 2333 Size: 524 4886 NspI r_CATG'y Cuts at: 1132 1229 2284 2768 4313 1132 Size: 97 1055 484 1545 2229 Fragments arranged by size: 2229 1545 1055 484 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5410 PciI A'CATG_T Cuts at: 4309 4309 Size: 5410 PflMI CCAn_nnn'nTGG Cuts at: 1108 2071 1108 Size: 963 4447 PmeI GTTT'AAAC Cuts at: 2714 2714 Size: 5410 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3479 3511 4992 5024 3479 Size: 32 1481 32 3865 Fragments arranged by size: 3865 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2464 2464 Size: 5410 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5410 PsiI TTA'TAA Cuts at: 2980 2980 Size: 5410 PspXI vC'TCGA_Gb Cuts at: 2411 2411 Size: 5410 PstI C_TGCA'G Cuts at: 830 2762 830 Size: 1932 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5410 SacI G_AGCT'C Cuts at: 2559 2559 Size: 5410 SacII CC_GC'GG Cuts at: 2330 2330 Size: 5410 SalI G'TCGA_C Cuts at: 1936 2752 1936 Size: 816 4594 SbfI CC_TGCA'GG Cuts at: 2762 2762 Size: 5410 ScaI AGT'ACT Cuts at: 3658 5370 3658 Size: 1712 3698 SexAI A'CCwGG_T Cuts at: 2070 2070 Size: 5410 SfcI C'TryA_G Cuts at: 826 1045 2758 3893 4574 4765 826 Size: 219 1713 1135 681 191 1471 Fragments arranged by size: 1713 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2305 2362 2551 2659 2305 Size: 57 189 108 5056 Fragments arranged by size: 5056 189 108 57 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5410 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5410 SmaI CCC'GGG Cuts at: 1872 2737 1872 Size: 865 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2411 3514 4415 4677 820 Size: 197 926 468 1103 901 262 1553 Fragments arranged by size: 1553 1103 926 901 468 262 197 SnaBI TAC'GTA Cuts at: 493 3201 493 Size: 2708 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5410 SphI G_CATG'C Cuts at: 1132 2768 1132 Size: 1636 3774 SspI AAT'ATT Cuts at: 5 52 3334 5 Size: 47 3282 2081 Fragments arranged by size: 3282 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5410 StyI C'CwwG_G Cuts at: 513 1065 2004 2849 513 Size: 552 939 845 3074 Fragments arranged by size: 3074 939 845 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2230 2578 3437 3454 3607 3792 4237 Size: 1255 650 348 859 17 153 185 445 Cuts at: 4237 325 Size: 1498 Fragments arranged by size: 1498 1255 859 650 445 348 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3656 5368 96 Size: 274 80 33 51 1443 1679 1712 138 Fragments arranged by size: 1712 1679 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5370 5402 5370 Size: 32 5378 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5410 XbaI T'CTAG_A Cuts at: 2746 2746 Size: 5410 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2635 2635 Size: 5410 XhoI C'TCGA_G Cuts at: 1943 2411 1943 Size: 468 4942 XmaI C'CCGG_G Cuts at: 1870 2735 1870 Size: 865 4545 XmnI GAAnn'nnTTC Cuts at: 3539 3539 Size: 5410 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2434 3218 276 Size: 53 83 186 936 900 784 2468 Fragments arranged by size: 2468 936 900 784 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI NruI NsiI PacI PfoI PmlI PspOMI PsrI PvuII RsrII SanDI SapI SfiI SrfI SwaI