################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BpmI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HapII Hin1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI MaeI MaeIII MamI MboI MfeI MflI MluI MroI MseI MspI MunI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RcaI RsrII SalI SapI Sau3AI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AspHI 67 GAGCTC G[AT]GC[AT]^C AvaI 1018 CCCGAG C^[CT]CG[GA]G BbsI 598 GTCTTC GAAGAC(2/6) BglI 571 GCCGTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 598 GTCTTC GAAGAC(2/6) BsaAI 640 TACGTG [CT]AC^GT[GA] BsiHKAI 67 GAGCTC G[AT]GC[AT]^C BsmFI 278 GTCCC GGGAC(10/14) BsoBI 1018 CCCGAG C^[CT]CG[GA]G Bsp1407I 620 TGTACA T^GTACA BspMI 206 ACCTGC ACCTGC(4/8) BsrDI 812 GCAATG GCAATG(2/0) BsrGI 620 TGTACA T^GTACA BstAPI 321 GCAGGGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC EcoNI 315 CCTGCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 89 CCAAGG C^C[AT][AT]GG HgaI 349 GCGTC GACGC(5/10) KspI 224 CCGCGG CCGC^GG MaeII 641 ACGT A^CGT NspI 672 GCATGT [GA]CATG^[CT] PshAI 723 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 67 GAGCTC GAGCT^C SacII 224 CCGCGG CCGC^GG ScaI 833 AGTACT AGT^ACT SfcI 824 CTGTAG C^T[GA][CT]AG SmlI 301 CTTGAG C^T[CT][GA]AG SspBI 620 TGTACA T^GTACA StyI 89 CCAAGG C^C[AT][AT]GG TaqI 586 TCGA T^CGA TthHB8I 586 TCGA T^CGA XcmI 704 CCACGCTCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AvaII 277 GGTCC G^G[AT]CC 525 GGACC BalI 356 TGGCCA TGG^CCA 368 TGGCCA BanII 67 GAGCTC G[GA]GC[CT]^C 578 GGGCTC BsaI 31 GAGACC GGTCTC(1/5) 700 GAGACC BsmAI 31 GAGAC GTCTC(1/5) 700 GAGAC BsrBI 558 CCGCTC CCGCTC(-3/-3) 382 GAGCGG EarI 235 CTCTTC CTCTTC(1/4) 256 CTCTTC Ksp632I 235 CTCTTC CTCTTC(1/4) 256 CTCTTC MluNI 356 TGGCCA TGG^CCA 368 TGGCCA MscI 356 TGGCCA TGG^CCA 368 TGGCCA MslI 372 CATCGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 388 CACCGCAGTG PvuII 409 CAGCTG CAG^CTG 678 CAGCTG ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AccII AciI AfaI AluI BanI BbvI BlpI BmyI Bpu1102I BsaJI BsgI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CelII CfoI Cfr13I DdeI DsaI EaeI EcoRII FauI Fnu4HI FokI HaeIII HhaI HinP1I HinfI ItaI MboII MnlI MspA1I MvaI MvnI MwoI NlaIII NlaIV PleI RsaI Sau96I ScrFI SfaNI TseI TspRI