(Circular) (Six-base) MAPSORT of: pFN21AE9559.seq Check: 6705 from: 1 to: 5758 pFN21AE9559 5758 bp With 182 enzymes: SgfI * August 25, 2011 15:07 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3568 278 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3101 1937 Size: 1164 4594 Acc65I G'GTAC_C Cuts at: 3079 3079 Size: 5758 AclI AA'CG_TT Cuts at: 1563 3885 4258 1563 Size: 2322 373 3063 Fragments arranged by size: 3063 2322 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3821 5205 5473 1632 Size: 2189 1384 268 1917 Fragments arranged by size: 2189 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5561 AflIII A'CryG_T Cuts at: 1203 4657 5634 1203 Size: 3454 977 1327 Fragments arranged by size: 3454 1327 977 AgeI A'CCGG_T Cuts at: 4589 4589 Size: 5758 AhdI GACnn_n'nnGTC Cuts at: 4487 4487 Size: 5758 AleI CACnn'nnGTG Cuts at: 1166 2397 1166 Size: 1231 4527 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5758 AlwNI CAG_nnn'CTG Cuts at: 3501 5073 3501 Size: 1572 4186 ApaLI G'TGCA_C Cuts at: 3815 4971 3815 Size: 1156 4602 ApoI r'AATT_y Cuts at: 1493 3067 3293 3445 5554 1493 Size: 1574 226 152 2109 1697 Fragments arranged by size: 2109 1697 1574 226 152 AseI AT'TA_AT Cuts at: 160 1032 4312 160 Size: 872 3280 1606 Fragments arranged by size: 3280 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 AvaI C'yCGr_G Cuts at: 1870 1943 3023 3083 1870 Size: 73 1080 60 4545 Fragments arranged by size: 4545 1080 73 60 BamHI G'GATC_C Cuts at: 3088 3455 3088 Size: 367 5391 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2391 2548 2999 3079 4534 Size: 325 217 627 604 157 451 80 1455 Cuts at: 4534 618 Size: 1842 Fragments arranged by size: 1842 1455 627 604 451 325 217 157 80 BanII G_rGCy'C Cuts at: 1391 2076 2587 3077 1391 Size: 685 511 490 4072 Fragments arranged by size: 4072 685 511 490 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2596 928 Size: 971 697 4090 Fragments arranged by size: 4090 971 697 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3949 3983 1903 Size: 34 2012 34 3678 Fragments arranged by size: 3678 2012 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3650 4860 3650 Size: 1210 4548 BclI T'GATC_A Cuts at: 1548 1548 Size: 5758 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2582 4369 136 Size: 107 122 71 631 605 910 1787 1525 Fragments arranged by size: 1787 1525 910 631 605 122 107 71 BglII A'GATC_T Cuts at: 5753 5753 Size: 5758 BlpI GC'TnA_GC Cuts at: 2075 2558 2714 3175 2075 Size: 483 156 461 4658 Fragments arranged by size: 4658 483 461 156 Bme1580I G_kGCm'C Cuts at: 1790 2527 3819 4975 1790 Size: 737 1292 1156 2573 Fragments arranged by size: 2573 1292 1156 737 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5758 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2126 3489 4447 Size: 416 53 83 399 24 709 1363 958 Cuts at: 4447 442 Size: 1753 Fragments arranged by size: 1753 1363 958 709 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5758 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2095 2881 2095 Size: 786 4972 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4418 1239 Size: 3179 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2327 3883 4748 5046 2327 Size: 1556 865 298 3039 Fragments arranged by size: 3039 1556 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2030 2699 4421 882 Size: 617 531 669 1722 2219 Fragments arranged by size: 2219 1722 669 617 531 BsaAI yAC'GTr Cuts at: 493 1204 2647 3549 493 Size: 711 1443 902 2702 Fragments arranged by size: 2702 1443 902 711 BsaBI GATnn'nnATC Cuts at: 5752 5752 Size: 5758 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3565 3947 4582 275 Size: 53 83 186 936 2032 382 635 1451 Fragments arranged by size: 2032 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4191 4589 4863 5010 5421 4191 Size: 398 274 147 411 4528 Fragments arranged by size: 4528 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5533 Fragments arranged by size: 5533 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5758 BseYI C'CCAG_C Cuts at: 4961 5457 4961 Size: 496 5262 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2402 2432 2681 1163 Size: 573 666 30 249 4240 Fragments arranged by size: 4240 666 573 249 30 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3208 3969 4997 1540 Size: 275 186 1207 761 1028 2301 Fragments arranged by size: 2301 1207 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2076 3819 3904 4975 2076 Size: 1743 85 1071 2859 Fragments arranged by size: 2859 1743 1071 85 BsmI GAATG_Cn' Cuts at: 3269 3362 3269 Size: 93 5665 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2527 2587 3077 3819 3904 4975 Size: 399 286 451 60 490 742 85 1071 Cuts at: 4975 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 490 451 399 286 85 60 BspEI T'CCGG_A Cuts at: 5421 5421 Size: 5758 BspHI T'CATG_A Cuts at: 3647 5377 3647 Size: 1730 4028 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2220 3113 844 Size: 1376 893 3489 Fragments arranged by size: 3489 1376 893 BsrBI CCG'CTC Cuts at: 1394 1823 2389 2565 3645 1394 Size: 429 566 176 1080 3507 Fragments arranged by size: 3507 1080 566 429 176 BsrDI GCAATG_nn' Cuts at: 65 1248 2824 4253 4427 65 Size: 1183 1576 1429 174 1396 Fragments arranged by size: 1576 1429 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4402 4589 1700 Size: 110 2592 187 2869 Fragments arranged by size: 2869 2592 187 110 BsrGI T'GTAC_A Cuts at: 96 2625 96 Size: 2529 3229 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5758 BssSI C'ACGA_G Cuts at: 3818 4830 3818 Size: 1012 4746 BstAPI GCAn_nnn'nTGC Cuts at: 794 2332 794 Size: 1538 4220 BstBI TT'CG_AA Cuts at: 4562 4562 Size: 5758 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5758 BstXI CCAn_nnnn'nTGG Cuts at: 2101 2158 2371 2383 2869 2101 Size: 57 213 12 486 4990 Fragments arranged by size: 4990 486 213 57 12 BstYI r'GATC_y Cuts at: 1972 3088 3455 3840 3857 5309 5395 5407 5753 Size: 1116 367 385 17 1452 86 12 346 Cuts at: 5753 1972 Size: 1977 Fragments arranged by size: 1977 1452 1116 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2229 2382 2434 513 Size: 552 939 225 153 52 3837 Fragments arranged by size: 3837 939 552 225 153 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2158 2362 487 Size: 683 56 932 204 3883 Fragments arranged by size: 3883 932 683 204 56 BtsI GCAGTG_nn' Cuts at: 2349 2366 2404 2512 3274 4068 4088 2349 Size: 17 38 108 762 794 20 4019 Fragments arranged by size: 4019 794 762 108 38 20 17 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5758 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5723 DraI TTT'AAA Cuts at: 3062 3062 Size: 5758 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4765 809 Size: 507 3449 1802 Fragments arranged by size: 3449 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2226 2361 2373 Size: 54 1425 50 275 18 396 135 12 Cuts at: 2373 2406 2437 2574 2853 3031 3205 4094 5723 Size: 33 31 137 279 178 174 889 1629 Cuts at: 5723 8 Size: 43 Fragments arranged by size: 1629 1425 889 396 279 275 178 174 137 135 54 50 43 33 31 18 12 EagI C'GGCC_G Cuts at: 1537 1812 3205 1537 Size: 275 1393 4090 Fragments arranged by size: 4090 1393 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2246 2267 3688 1353 Size: 893 21 1421 3423 Fragments arranged by size: 3423 1421 893 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2912 2939 4345 4719 4865 387 Size: 1064 138 1323 27 1406 374 146 1280 Fragments arranged by size: 1406 1323 1280 1064 374 146 138 27 EcoICRI GAG'CTC Cuts at: 2074 2074 Size: 5758 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3821 4418 5205 5473 1239 Size: 393 2189 597 787 268 1524 Fragments arranged by size: 2189 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2324 2324 Size: 5758 EcoRI G'AATT_C Cuts at: 3067 3445 3067 Size: 378 5380 FspI TGC'GCA Cuts at: 4264 4264 Size: 5758 HaeII r_GCGC'y Cuts at: 1678 4905 1678 Size: 3227 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2522 2554 4399 4431 4473 Size: 32 135 32 950 32 1845 32 42 Cuts at: 4473 4505 1373 Size: 32 2626 Fragments arranged by size: 2626 1845 950 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3102 3348 669 Size: 988 281 1164 246 3079 Fragments arranged by size: 3079 1164 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5758 HpaI GTT'AAC Cuts at: 3348 3348 Size: 5758 KpnI G_GTAC'C Cuts at: 3083 3083 Size: 5758 MfeI C'AATT_G Cuts at: 3357 3357 Size: 5758 MluI A'CGCG_T Cuts at: 5634 5634 Size: 5758 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2205 4872 5056 1305 Size: 900 2667 184 2007 Fragments arranged by size: 2667 2007 900 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2363 2375 10 Size: 54 1425 343 531 12 3393 Fragments arranged by size: 3393 1425 531 343 54 12 MslI CAynn'nnrTG Cuts at: 518 1166 2381 2397 3716 4075 4234 518 Size: 648 1215 16 1319 359 159 2042 Fragments arranged by size: 2042 1319 1215 648 359 159 16 MspA1I CmG'CkG Cuts at: 1606 2231 2416 2535 2685 3174 3851 4999 5244 Size: 625 185 119 150 489 677 1148 245 Cuts at: 5244 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 625 489 245 185 150 119 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4267 Fragments arranged by size: 4267 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5758 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5758 NotI GC'GGCC_GC Cuts at: 3205 3205 Size: 5758 NspI r_CATG'y Cuts at: 1132 1229 2681 3116 4661 1132 Size: 97 1452 435 1545 2229 Fragments arranged by size: 2229 1545 1452 435 97 PasI CC'CwG_GG Cuts at: 1348 2479 2992 1348 Size: 1131 513 4114 Fragments arranged by size: 4114 1131 513 PciI A'CATG_T Cuts at: 4657 4657 Size: 5758 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5758 PfoI T'CCnGG_A Cuts at: 2790 2790 Size: 5758 PmeI GTTT'AAAC Cuts at: 3062 3062 Size: 5758 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2300 2332 3827 3859 5340 5372 2300 Size: 32 1495 32 1481 32 2686 Fragments arranged by size: 2686 1495 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2732 1613 Size: 1119 4639 PsiI TTA'TAA Cuts at: 3328 3328 Size: 5758 PstI C_TGCA'G Cuts at: 830 3110 830 Size: 2280 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5758 PvuII CAG'CTG Cuts at: 2416 2685 2416 Size: 269 5489 SacI G_AGCT'C Cuts at: 2076 2076 Size: 5758 SacII CC_GC'GG Cuts at: 2232 2232 Size: 5758 SalI G'TCGA_C Cuts at: 1936 3100 1936 Size: 1164 4594 SbfI CC_TGCA'GG Cuts at: 3110 3110 Size: 5758 ScaI AGT'ACT Cuts at: 2840 4006 5718 2840 Size: 1166 1712 2880 Fragments arranged by size: 2880 1712 1166 SfcI C'TryA_G Cuts at: 826 1045 2829 3106 4241 4922 5113 826 Size: 219 1784 277 1135 681 191 1471 Fragments arranged by size: 1784 1471 1135 681 277 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5758 SmaI CCC'GGG Cuts at: 1872 3085 1872 Size: 1213 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2306 3862 4763 5025 820 Size: 197 926 363 1556 901 262 1553 Fragments arranged by size: 1556 1553 926 901 363 262 197 SnaBI TAC'GTA Cuts at: 493 3549 493 Size: 3056 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5758 SphI G_CATG'C Cuts at: 1132 3116 1132 Size: 1984 3774 SspI AAT'ATT Cuts at: 5 52 3682 5 Size: 47 3630 2081 Fragments arranged by size: 3630 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5758 StyI C'CwwG_G Cuts at: 513 1065 2004 2094 3197 513 Size: 552 939 90 1103 3074 Fragments arranged by size: 3074 1103 939 552 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2458 3051 3785 3802 3955 4140 4585 Size: 1255 878 593 734 17 153 185 445 Cuts at: 4585 325 Size: 1498 Fragments arranged by size: 1498 1255 878 734 593 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2625 2838 2886 Size: 274 80 33 51 1443 648 213 48 Cuts at: 2886 4004 5716 96 Size: 1118 1712 138 Fragments arranged by size: 1712 1443 1118 648 274 213 138 80 51 48 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2966 2998 5718 5750 2966 Size: 32 2720 32 2974 Fragments arranged by size: 2974 2720 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5758 XbaI T'CTAG_A Cuts at: 3094 3094 Size: 5758 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2716 2011 Size: 705 5053 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5758 XmaI C'CCGG_G Cuts at: 1870 3083 1870 Size: 1213 4545 XmnI GAAnn'nnTTC Cuts at: 3887 3887 Size: 5758 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3566 276 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI