(Linear) (Six-base) MAPSORT of: KIEE9559.seq Check: 2544 from: 1 to: 1053 KIEE9559.orf With 182 enzymes: SgfI * August 25, 2011 14:40 .. AleI CACnn'nnGTG Cuts at: 0 392 1053 Size: 392 661 AvaI C'yCGr_G Cuts at: 0 1018 1053 Size: 1018 35 BanI G'GyrC_C Cuts at: 0 386 543 994 1053 Size: 386 157 451 59 Fragments arranged by size: 451 386 157 59 BanII G_rGCy'C Cuts at: 0 71 582 1053 Size: 71 511 471 Fragments arranged by size: 511 471 71 BbsI GAAGACnn'nnnn_ Cuts at: 0 591 1053 Size: 591 462 BglI GCCn_nnn'nGGC Cuts at: 0 577 1053 Size: 577 476 BlpI GC'TnA_GC Cuts at: 0 70 553 709 1053 Size: 70 483 156 344 Fragments arranged by size: 483 344 156 70 Bme1580I G_kGCm'C Cuts at: 0 522 1053 Size: 522 531 BmrI ACTGGGnnnn_n' Cuts at: 0 121 1053 Size: 121 932 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 90 876 1053 Size: 90 786 177 Fragments arranged by size: 786 177 90 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 322 1053 Size: 322 731 BsaI GGTCTCn'nnnn_ Cuts at: 0 25 694 1053 Size: 25 669 359 Fragments arranged by size: 669 359 25 BsaAI yAC'GTr Cuts at: 0 642 1053 Size: 642 411 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 397 427 676 1053 Size: 397 30 249 377 Fragments arranged by size: 397 377 249 30 BsiHKAI G_wGCw'C Cuts at: 0 71 1053 Size: 71 982 Bsp1286I G_dGCh'C Cuts at: 0 71 522 582 1053 Size: 71 451 60 471 Fragments arranged by size: 471 451 71 60 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 215 1053 Size: 215 838 BsrBI CCG'CTC Cuts at: 0 384 560 1053 Size: 384 176 493 Fragments arranged by size: 493 384 176 BsrDI GCAATG_nn' Cuts at: 0 819 1053 Size: 819 234 BsrGI T'GTAC_A Cuts at: 0 620 1053 Size: 620 433 BstAPI GCAn_nnn'nTGC Cuts at: 0 327 1053 Size: 327 726 BstXI CCAn_nnnn'nTGG Cuts at: 0 96 153 366 378 864 1053 Size: 96 57 213 12 486 189 Fragments arranged by size: 486 213 189 96 57 12 BtgI C'CryG_G Cuts at: 0 224 377 429 1053 Size: 224 153 52 624 Fragments arranged by size: 624 224 153 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 153 357 1053 Size: 153 204 696 Fragments arranged by size: 696 204 153 BtsI GCAGTG_nn' Cuts at: 0 344 361 399 507 1053 Size: 344 17 38 108 546 Fragments arranged by size: 546 344 108 38 17 EaeI y'GGCC_r Cuts at: 0 221 356 368 401 432 569 848 1026 Size: 221 135 12 33 31 137 279 178 Cuts at: 1026 1053 Size: 27 Fragments arranged by size: 279 221 178 137 135 33 31 27 12 EarI CTCTTCn'nnn_ Cuts at: 0 241 262 1053 Size: 241 21 791 Fragments arranged by size: 791 241 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 907 934 1053 Size: 907 27 119 Fragments arranged by size: 907 119 27 EcoICRI GAG'CTC Cuts at: 0 69 1053 Size: 69 984 EcoNI CCTnn'n_nnAGG Cuts at: 0 319 1053 Size: 319 734 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 517 549 1053 Size: 517 32 504 Fragments arranged by size: 517 504 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 200 1053 Size: 200 853 MscI TGG'CCA Cuts at: 0 358 370 1053 Size: 358 12 683 Fragments arranged by size: 683 358 12 MslI CAynn'nnrTG Cuts at: 0 376 392 1053 Size: 376 16 661 Fragments arranged by size: 661 376 16 MspA1I CmG'CkG Cuts at: 0 226 411 530 680 1053 Size: 226 185 119 150 373 Fragments arranged by size: 373 226 185 150 119 NspI r_CATG'y Cuts at: 0 676 1053 Size: 676 377 PasI CC'CwG_GG Cuts at: 0 474 987 1053 Size: 474 513 66 Fragments arranged by size: 513 474 66 PfoI T'CCnGG_A Cuts at: 0 785 1053 Size: 785 268 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 295 327 1053 Size: 295 32 726 Fragments arranged by size: 726 295 32 PshAI GACnn'nnGTC Cuts at: 0 727 1053 Size: 727 326 PvuII CAG'CTG Cuts at: 0 411 680 1053 Size: 411 269 373 Fragments arranged by size: 411 373 269 SacI G_AGCT'C Cuts at: 0 71 1053 Size: 71 982 SacII CC_GC'GG Cuts at: 0 227 1053 Size: 227 826 ScaI AGT'ACT Cuts at: 0 835 1053 Size: 835 218 SfcI C'TryA_G Cuts at: 0 824 1053 Size: 824 229 SmlI C'TyrA_G Cuts at: 0 301 1053 Size: 301 752 StyI C'CwwG_G Cuts at: 0 89 1053 Size: 89 964 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 453 1046 1053 Size: 453 593 7 Fragments arranged by size: 593 453 7 TatI w'GTAC_w Cuts at: 0 620 833 881 1053 Size: 620 213 48 172 Fragments arranged by size: 620 213 172 48 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 961 993 1053 Size: 961 32 60 Fragments arranged by size: 961 60 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 711 1053 Size: 711 342 Enzymes that do cut: AleI AvaI BanI BanII BbsI BglI BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrGI BstAPI BstXI BtgI BtgZI BtsI EaeI EarI EciI EcoICRI EcoNI Hin4I MmeI MscI MslI MspA1I NspI PasI PfoI PpiI PshAI PvuII SacI SacII ScaI SfcI SmlI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmtI BpmI Bpu10I BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrFI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI Eco57MI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PmeI PmlI PpuMI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI