################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanII BbrPI BbsI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HindIII HpaI KpnI MamI MfeI MluI MroI MseI MunI NciI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI ScaI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StyI SwaI Tru9I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 61 GACGTC GACGT^C AcsI 645 GAATTT [GA]^AATT[CT] AfaI 1112 GTAC GT^AC Aor51HI 952 AGCGCT AGC^GCT ApoI 645 GAATTT [GA]^AATT[CT] AvaI 286 CCCGAG C^[CT]CG[GA]G BbvCI 268 GCTGAGG CCTCAGC(-5/-2) BcgI 662 CGACGGAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 864 GGATAC GTATCC(6/5) BsaWI 797 TCCGGT [AT]^CCGG[AT] BsgI 279 GTGCAG GTGCAG(16/14) BsmI 209 GCATTC GAATGC(1/-1) BsoBI 286 CCCGAG C^[CT]CG[GA]G BsrBI 308 GAGCGG CCGCTC(-3/-3) BsrFI 322 GCCGGC [GA]^CCGG[CT] BssSI 54 CACGAG CACGAG(-5/-1) Cfr10I 322 GCCGGC [GA]^CCGG[CT] DraII 881 GGGTCCC [GA]G^G[ACGT]CC[CT] DraIII 335 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 952 AGCGCT AGC^GCT EcoO109I 881 GGGTCCC [GA]G^G[ACGT]CC[CT] HincII 909 GTCAAC GT[CT]^[GA]AC HindII 909 GTCAAC GT[CT]^[GA]AC KspI 40 CCGCGG CCGC^GG MaeIII 466 GTCAC ^GT[ACGT]AC MslI 561 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 322 GCCGGC GCC^GGC NgoMIV 322 GCCGGC G^CCGGC PflMI 93 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 981 GACTC GAGTC(4/5) PpuMI 881 GGGTCCC [GA]G^G[AT]CC[CT] PvuII 900 CAGCTG CAG^CTG RsaI 1112 GTAC GT^AC SacII 40 CCGCGG CCGC^GG SapI 730 GAAGAGC GCTCTTC(1/4) SexAI 336 ACCAGGT A^CC[AT]GGT SphI 123 GCATGC GCATG^C TfiI 794 GAATC G^A[AT]TC Tsp45I 466 GTCAC ^GT[GC]AC Van91I 93 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AlwI 223 GATCC GGATC(4/5) 399 GATCC AlwNI 94 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG 238 CAGCTCCTG AocI 243 CCTGAGG CC^T[ACGT]AGG 511 CCTAAGG AvaII 882 GGTCC G^G[AT]CC 934 GGACC BanI 44 GGCGCC G^G[CT][GA]CC 535 GGCGCC BbeI 44 GGCGCC GGCGC^C 535 GGCGCC BfaI 424 CTAG C^TAG 917 CTAG BsaI 1088 GAGACC GGTCTC(1/5) 768 GGTCTC BspMI 1065 ACCTGC ACCTGC(4/8) 1080 ACCTGC BsrI 776 ACTGG ACTGG(1/-1) 1109 CCAGT BstYI 222 AGATCC [GA]^GATC[CT] 398 AGATCC Bsu36I 243 CCTGAGG CC^T[ACGT]AGG 511 CCTAAGG Eco81I 243 CCTGAGG CC^T[ACGT]AGG 511 CCTAAGG HgaI 232 GACGC GACGC(5/10) 961 GACGC HinfI 794 GAATC G^A[ACGT]TC 981 GACTC KasI 44 GGCGCC G^GCGCC 535 GGCGCC MaeI 424 CTAG C^TAG 917 CTAG MaeII 62 ACGT A^CGT 464 ACGT MflI 222 AGATCC [GA]^GATC[CT] 398 AGATCC NarI 44 GGCGCC GG^CGCC 535 GGCGCC NspI 123 GCATGC [GA]CATG^[CT] 257 ACATGC PstI 67 CTGCAG CTGCA^G 1021 CTGCAG SfoI 44 GGCGCC GGC^GCC 535 GGCGCC StuI 440 AGGCCT AGG^CCT 499 AGGCCT TaqI 50 TCGA T^CGA 623 TCGA Tsp509I 133 AATT ^AATT 646 AATT TspRI 191 CAGTG CA[GC]TG[ACGT][ACGT]^ 596 CAGTG TthHB8I 50 TCGA T^CGA 623 TCGA XcmI 725 CCAAAGAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 996 CCAGCCTCCTCCTGG XhoII 222 AGATCC [GA]^GATC[CT] 398 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AccII AciI AcyI AluI AspHI BalI BbvI BmyI BpmI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoNI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI Ksp632I MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SfcI TseI