(Circular) (Six-base) MAPSORT of: pFN21AB9713.seq Check: 3767 from: 1 to: 5827 pFN21AB9713 5827 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3094 3094 Size: 5827 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2070 3637 278 Size: 53 83 186 936 534 1567 2468 Fragments arranged by size: 2468 1567 936 534 186 83 53 AccI GT'mk_AC Cuts at: 1937 3170 1937 Size: 1233 4594 Acc65I G'GTAC_C Cuts at: 3148 3148 Size: 5827 AclI AA'CG_TT Cuts at: 1563 3954 4327 1563 Size: 2391 373 3063 Fragments arranged by size: 3063 2391 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3890 5274 5542 1632 Size: 2258 1384 268 1917 Fragments arranged by size: 2258 1917 1384 268 AfeI AGC'GCT Cuts at: 2959 2959 Size: 5827 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5630 AflIII A'CryG_T Cuts at: 1203 4726 5703 1203 Size: 3523 977 1327 Fragments arranged by size: 3523 1327 977 AgeI A'CCGG_T Cuts at: 4658 4658 Size: 5827 AhdI GACnn_n'nnGTC Cuts at: 4556 4556 Size: 5827 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5827 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5827 AlwNI CAG_nnn'CTG Cuts at: 2104 2248 3570 5142 2104 Size: 144 1322 1572 2789 Fragments arranged by size: 2789 1572 1322 144 ApaLI G'TGCA_C Cuts at: 3884 5040 3884 Size: 1156 4671 ApoI r'AATT_y Cuts at: 1493 2650 3136 3362 3514 5623 1493 Size: 1157 486 226 152 2109 1697 Fragments arranged by size: 2109 1697 1157 486 226 152 AseI AT'TA_AT Cuts at: 160 1032 4381 160 Size: 872 3349 1606 Fragments arranged by size: 3349 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 AvaI C'yCGr_G Cuts at: 1870 1943 2291 3152 1870 Size: 73 348 861 4545 Fragments arranged by size: 4545 861 348 73 BamHI G'GATC_C Cuts at: 3157 3524 3157 Size: 367 5460 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2049 2540 3148 4603 618 Size: 325 217 627 262 491 608 1455 1842 Fragments arranged by size: 1842 1455 627 608 491 325 262 217 BanII G_rGCy'C Cuts at: 1391 3146 1391 Size: 1755 4072 BbeI G_GCGC'C Cuts at: 2053 2544 2053 Size: 491 5336 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4856 BbvCI CC'TCA_GC Cuts at: 2274 2274 Size: 5827 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2656 2690 4018 4052 1903 Size: 34 719 34 1328 34 3678 Fragments arranged by size: 3678 1328 719 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2863 3719 4929 2863 Size: 856 1210 3761 Fragments arranged by size: 3761 1210 856 BclI T'GATC_A Cuts at: 1548 1548 Size: 5827 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4438 136 Size: 107 122 71 631 605 2766 1525 Fragments arranged by size: 2766 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5822 5822 Size: 5827 BlpI GC'TnA_GC Cuts at: 3244 3244 Size: 5827 Bme1580I G_kGCm'C Cuts at: 1790 3888 5044 1790 Size: 2098 1156 2573 Fragments arranged by size: 2573 2098 1156 BmgBI CAC'GTC Cuts at: 1318 2470 1318 Size: 1152 4675 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3558 4516 442 Size: 416 53 83 399 24 2141 958 1753 Fragments arranged by size: 2141 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5827 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2468 2984 3097 3111 4487 1239 Size: 1229 516 113 14 1376 2579 Fragments arranged by size: 2579 1376 1229 516 113 14 Bpu10I CC'TnA_GC Cuts at: 2274 2274 Size: 5827 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3952 4817 5115 3952 Size: 865 298 4664 Fragments arranged by size: 4664 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2779 3087 4490 882 Size: 617 1280 308 1403 2219 Fragments arranged by size: 2219 1403 1280 617 308 BsaAI yAC'GTr Cuts at: 493 1204 3618 493 Size: 711 2414 2702 Fragments arranged by size: 2702 2414 711 BsaBI GATnn'nnATC Cuts at: 5821 5821 Size: 5827 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2050 2067 2238 2541 Size: 53 83 186 936 517 17 171 303 Cuts at: 2541 2967 3634 4016 4651 275 Size: 426 667 382 635 1451 Fragments arranged by size: 1451 936 667 635 517 426 382 303 186 171 83 53 17 BsaWI w'CCGG_w Cuts at: 2802 4260 4658 4932 5079 5490 2802 Size: 1458 398 274 147 411 3139 Fragments arranged by size: 3139 1458 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2301 2331 1178 Size: 30 165 30 898 30 4674 Fragments arranged by size: 4674 898 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2322 2325 2471 2923 2997 1186 Size: 1136 3 146 452 74 4016 Fragments arranged by size: 4016 1136 452 146 74 3 BseYI C'CCAG_C Cuts at: 2241 2717 2954 2960 5030 5526 2241 Size: 476 237 6 2070 496 2542 Fragments arranged by size: 2542 2070 496 476 237 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2305 1163 Size: 573 569 4685 Fragments arranged by size: 4685 573 569 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3277 4038 5066 1540 Size: 275 186 1276 761 1028 2301 Fragments arranged by size: 2301 1276 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2175 2376 2591 3888 3973 5044 2175 Size: 201 215 1297 85 1071 2958 Fragments arranged by size: 2958 1297 1071 215 201 85 BsmI GAATG_Cn' Cuts at: 2214 3338 3431 2214 Size: 1124 93 4610 Fragments arranged by size: 4610 1124 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2175 2376 2591 3146 3888 3973 5044 Size: 399 385 201 215 555 742 85 1071 Cuts at: 5044 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 555 399 385 215 201 85 BspEI T'CCGG_A Cuts at: 5490 5490 Size: 5827 BspHI T'CATG_A Cuts at: 3716 5446 3716 Size: 1730 4097 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3079 3094 3182 844 Size: 2235 15 88 3489 Fragments arranged by size: 3489 2235 88 15 BsrBI CCG'CTC Cuts at: 1394 1823 2315 3714 1394 Size: 429 492 1399 3507 Fragments arranged by size: 3507 1399 492 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4322 4496 65 Size: 1183 3074 174 1396 Fragments arranged by size: 3074 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2327 4471 4658 1700 Size: 110 517 2144 187 2869 Fragments arranged by size: 2869 2144 517 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5827 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5827 BssSI C'ACGA_G Cuts at: 2059 3887 4899 2059 Size: 1828 1012 2987 Fragments arranged by size: 2987 1828 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5827 BstBI TT'CG_AA Cuts at: 4631 4631 Size: 5827 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5827 BstYI r'GATC_y Cuts at: 1972 2227 2403 3157 3524 3909 3926 5378 5464 Size: 255 176 754 367 385 17 1452 86 Cuts at: 5464 5476 5822 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 754 385 367 346 255 176 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2249 2517 2249 Size: 268 5559 BtgI C'CryG_G Cuts at: 513 1065 2004 2045 2209 2874 513 Size: 552 939 41 164 665 3466 Fragments arranged by size: 3466 939 665 552 164 41 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2016 487 Size: 683 56 790 4298 Fragments arranged by size: 4298 790 683 56 BtsI GCAGTG_nn' Cuts at: 2607 3343 4137 4157 2607 Size: 736 794 20 4277 Fragments arranged by size: 4277 794 736 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5827 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2863 2898 529 Size: 35 2299 35 3458 Fragments arranged by size: 3458 2299 35 35 DraI TTT'AAA Cuts at: 3131 3131 Size: 5827 DraIII CAC_nnn'GTG Cuts at: 2345 2345 Size: 5827 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4834 809 Size: 507 3518 1802 Fragments arranged by size: 3518 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2085 2319 Size: 54 1425 50 275 18 177 78 234 Cuts at: 2319 2742 3274 4163 5792 8 Size: 423 532 889 1629 43 Fragments arranged by size: 1629 1425 889 532 423 275 234 177 78 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3274 1537 Size: 275 1462 4090 Fragments arranged by size: 4090 1462 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2291 2299 2730 2829 3757 1353 Size: 938 8 431 99 928 3423 Fragments arranged by size: 3423 938 928 431 99 8 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2662 4414 4788 4934 387 Size: 1064 138 1073 1752 374 146 1280 Fragments arranged by size: 1752 1280 1073 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2468 2984 3097 3111 3890 4487 5274 Size: 393 836 516 113 14 779 597 787 Cuts at: 5274 5542 1239 Size: 268 1524 Fragments arranged by size: 1524 836 787 779 597 516 393 268 113 14 EcoNI CCTnn'n_nnAGG Cuts at: 2108 2483 3015 2108 Size: 375 532 4920 Fragments arranged by size: 4920 532 375 EcoO109I rG'GnC_Cy Cuts at: 2887 2887 Size: 5827 EcoRI G'AATT_C Cuts at: 3136 3514 3136 Size: 378 5449 FspI TGC'GCA Cuts at: 4333 4333 Size: 5827 HaeII r_GCGC'y Cuts at: 1678 2053 2544 2565 2961 4974 1678 Size: 375 491 21 396 2013 2531 Fragments arranged by size: 2531 2013 491 396 375 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4468 4500 4542 4574 1373 Size: 32 135 32 2896 32 42 32 2626 Fragments arranged by size: 2896 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2916 3171 3417 669 Size: 988 281 978 255 246 3079 Fragments arranged by size: 3079 988 978 281 255 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5827 HpaI GTT'AAC Cuts at: 3417 3417 Size: 5827 KasI G'GCGC_C Cuts at: 2049 2540 2049 Size: 491 5336 KpnI G_GTAC'C Cuts at: 3152 3152 Size: 5827 MfeI C'AATT_G Cuts at: 3426 3426 Size: 5827 MluI A'CGCG_T Cuts at: 5703 5703 Size: 5827 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2431 4941 5125 1305 Size: 1126 2510 184 2007 Fragments arranged by size: 2510 2007 1126 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2087 2321 2744 10 Size: 54 1425 343 255 234 423 3093 Fragments arranged by size: 3093 1425 423 343 255 234 54 MslI CAynn'nnrTG Cuts at: 518 1166 2570 3785 4144 4303 518 Size: 648 1404 1215 359 159 2042 Fragments arranged by size: 2042 1404 1215 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2047 2907 2950 3243 3920 5068 5313 1606 Size: 441 860 43 293 677 1148 245 2120 Fragments arranged by size: 2120 1148 860 677 441 293 245 43 NaeI GCC'GGC Cuts at: 2329 2329 Size: 5827 NarI GG'CG_CC Cuts at: 2050 2541 2050 Size: 491 5336 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4336 Fragments arranged by size: 4336 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5827 NgoMIV G'CCGG_C Cuts at: 2327 2327 Size: 5827 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5827 NotI GC'GGCC_GC Cuts at: 3274 3274 Size: 5827 NspI r_CATG'y Cuts at: 1132 1229 2132 2266 3185 4730 1132 Size: 97 903 134 919 1545 2229 Fragments arranged by size: 2229 1545 919 903 134 97 PasI CC'CwG_GG Cuts at: 1348 2891 3040 1348 Size: 1543 149 4135 Fragments arranged by size: 4135 1543 149 PciI A'CATG_T Cuts at: 4726 4726 Size: 5827 PflMI CCAn_nnn'nTGG Cuts at: 1108 2104 1108 Size: 996 4831 PfoI T'CCnGG_A Cuts at: 2484 2484 Size: 5827 PmeI GTTT'AAAC Cuts at: 3131 3131 Size: 5827 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3896 3928 5409 5441 3896 Size: 32 1481 32 4282 Fragments arranged by size: 4282 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2887 2887 Size: 5827 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5827 PsiI TTA'TAA Cuts at: 3397 3397 Size: 5827 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2696 2728 2696 Size: 32 5795 PstI C_TGCA'G Cuts at: 830 2076 3030 3179 830 Size: 1246 954 149 3478 Fragments arranged by size: 3478 1246 954 149 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5827 PvuII CAG'CTG Cuts at: 2907 2907 Size: 5827 SacII CC_GC'GG Cuts at: 2048 2048 Size: 5827 SalI G'TCGA_C Cuts at: 1936 3169 1936 Size: 1233 4594 SanDI GG'GwC_CC Cuts at: 2887 2887 Size: 5827 SapI GCTCTTCn'nnn_ Cuts at: 2730 2730 Size: 5827 SbfI CC_TGCA'GG Cuts at: 3179 3179 Size: 5827 ScaI AGT'ACT Cuts at: 4075 5787 4075 Size: 1712 4115 SexAI A'CCwGG_T Cuts at: 2341 2341 Size: 5827 SfcI C'TryA_G Cuts at: 826 1045 2072 2383 3026 3175 4310 4991 5182 Size: 219 1027 311 643 149 1135 681 191 Cuts at: 5182 826 Size: 1471 Fragments arranged by size: 1471 1135 1027 681 643 311 219 191 149 SfoI GGC'GCC Cuts at: 2051 2542 2051 Size: 491 5336 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5827 SmaI CCC'GGG Cuts at: 1872 3154 1872 Size: 1282 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3931 4832 5094 820 Size: 197 926 1988 901 262 1553 Fragments arranged by size: 1988 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3618 493 Size: 3125 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5827 SphI G_CATG'C Cuts at: 1132 2132 3185 1132 Size: 1000 1053 3774 Fragments arranged by size: 3774 1053 1000 SspI AAT'ATT Cuts at: 5 52 3751 5 Size: 47 3699 2081 Fragments arranged by size: 3699 2081 47 StuI AGG'CCT Cuts at: 1517 2447 2506 1517 Size: 930 59 4838 Fragments arranged by size: 4838 930 59 StyI C'CwwG_G Cuts at: 513 1065 2004 3266 513 Size: 552 939 1262 3074 Fragments arranged by size: 3074 1262 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3854 3871 4024 4209 4654 325 Size: 1255 2274 17 153 185 445 1498 Fragments arranged by size: 2274 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3116 4073 5785 Size: 274 80 33 51 1443 1139 957 1712 Cuts at: 5785 96 Size: 138 Fragments arranged by size: 1712 1443 1139 957 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2301 2333 5787 5819 2301 Size: 32 3454 32 2309 Fragments arranged by size: 3454 2309 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5827 XbaI T'CTAG_A Cuts at: 3163 3163 Size: 5827 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2737 3008 2737 Size: 271 5556 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5827 XmaI C'CCGG_G Cuts at: 1870 3152 1870 Size: 1282 4545 XmnI GAAnn'nnTTC Cuts at: 3956 3956 Size: 5827 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2068 3635 276 Size: 53 83 186 936 534 1567 2468 Fragments arranged by size: 2468 1567 936 534 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SfiI SrfI SwaI