(Circular) (Six-base) MAPSORT of: pFN21AE4569.seq Check: 803 from: 1 to: 6877 pFN21AE4569 6877 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2289 4687 278 Size: 53 83 186 936 753 2398 2468 Fragments arranged by size: 2468 2398 936 753 186 83 53 AccI GT'mk_AC Cuts at: 1937 3447 4220 1937 Size: 1510 773 4594 Fragments arranged by size: 4594 1510 773 Acc65I G'GTAC_C Cuts at: 3073 4198 3073 Size: 1125 5752 AclI AA'CG_TT Cuts at: 1563 5004 5377 1563 Size: 3441 373 3063 Fragments arranged by size: 3441 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3481 3498 4940 6324 6592 1632 Size: 1849 17 1442 1384 268 1917 Fragments arranged by size: 1917 1849 1442 1384 268 17 AfeI AGC'GCT Cuts at: 2585 2585 Size: 6877 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6680 AflIII A'CryG_T Cuts at: 1203 2115 3411 3459 5776 6753 1203 Size: 912 1296 48 2317 977 1327 Fragments arranged by size: 2317 1327 1296 977 912 48 AgeI A'CCGG_T Cuts at: 5708 5708 Size: 6877 AhdI GACnn_n'nnGTC Cuts at: 2557 3886 3943 5606 2557 Size: 1329 57 1663 3828 Fragments arranged by size: 3828 1663 1329 57 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6877 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6877 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2069 2101 2069 Size: 32 6845 AlwNI CAG_nnn'CTG Cuts at: 4620 6192 4620 Size: 1572 5305 ApaI G_GGCC'C Cuts at: 4013 4013 Size: 6877 ApaLI G'TGCA_C Cuts at: 4934 6090 4934 Size: 1156 5721 ApoI r'AATT_y Cuts at: 1493 4186 4412 4564 6673 1493 Size: 2693 226 152 2109 1697 Fragments arranged by size: 2693 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5431 160 Size: 872 4399 1606 Fragments arranged by size: 4399 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6877 AvaI C'yCGr_G Cuts at: 1870 1943 2319 2344 2505 3237 3842 4202 1870 Size: 73 376 25 161 732 605 360 4545 Fragments arranged by size: 4545 732 605 376 360 161 73 25 AvrII C'CTAG_G Cuts at: 3068 3068 Size: 6877 BamHI G'GATC_C Cuts at: 2694 3042 4207 4574 2694 Size: 348 1165 367 4997 Fragments arranged by size: 4997 1165 367 348 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3073 3256 3364 4198 5653 Size: 325 217 627 1286 183 108 834 1455 Cuts at: 5653 618 Size: 1842 Fragments arranged by size: 1842 1455 1286 834 627 325 217 183 108 BanII G_rGCy'C Cuts at: 1391 2513 4013 4196 1391 Size: 1122 1500 183 4072 Fragments arranged by size: 4072 1500 1122 183 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3822 4069 928 Size: 971 1923 247 3736 Fragments arranged by size: 3736 1923 971 247 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2831 2865 3413 3447 5068 Size: 34 50 34 810 34 548 34 1621 Cuts at: 5068 5102 1903 Size: 34 3678 Fragments arranged by size: 3678 1621 810 548 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4769 5979 4769 Size: 1210 5667 BclI T'GATC_A Cuts at: 1548 1548 Size: 6877 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2253 2538 2598 Size: 107 122 71 631 605 581 285 60 Cuts at: 2598 5488 136 Size: 2890 1525 Fragments arranged by size: 2890 1525 631 605 581 285 122 107 71 60 BglII A'GATC_T Cuts at: 6872 6872 Size: 6877 BlpI GC'TnA_GC Cuts at: 4294 4294 Size: 6877 Bme1580I G_kGCm'C Cuts at: 1790 2793 3271 3659 4013 4938 6094 1790 Size: 1003 478 388 354 925 1156 2573 Fragments arranged by size: 2573 1156 1003 925 478 388 354 BmgBI CAC'GTC Cuts at: 1318 2047 3412 3460 1318 Size: 729 1365 48 4735 Fragments arranged by size: 4735 1365 729 48 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3565 4608 5566 Size: 416 53 83 399 24 2148 1043 958 Cuts at: 5566 442 Size: 1753 Fragments arranged by size: 2148 1753 1043 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6877 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3709 5537 1239 Size: 2470 1828 2579 Fragments arranged by size: 2579 2470 1828 Bpu10I CC'TnA_GC Cuts at: 4014 4014 Size: 6877 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3800 5002 5867 6165 3800 Size: 1202 865 298 4512 Fragments arranged by size: 4512 1202 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2453 4148 5540 882 Size: 617 954 1695 1392 2219 Fragments arranged by size: 2219 1695 1392 954 617 BsaAI yAC'GTr Cuts at: 493 1204 3736 4668 493 Size: 711 2532 932 2702 Fragments arranged by size: 2702 2532 932 711 BsaBI GATnn'nnATC Cuts at: 6871 6871 Size: 6877 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2286 2558 2835 4684 Size: 53 83 186 936 753 272 277 1849 Cuts at: 4684 5066 5701 275 Size: 382 635 1451 Fragments arranged by size: 1849 1451 936 753 635 382 277 272 186 83 53 BsaWI w'CCGG_w Cuts at: 2845 3039 3466 5310 5708 5982 6129 6540 2845 Size: 194 427 1844 398 274 147 411 3182 Fragments arranged by size: 3182 1844 427 411 398 274 194 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2069 2099 2747 2777 3749 Size: 30 165 30 666 30 648 30 972 Cuts at: 3749 3779 1178 Size: 30 4276 Fragments arranged by size: 4276 972 666 648 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6877 BseYI C'CCAG_C Cuts at: 2153 2606 2679 6080 6576 2153 Size: 453 73 3401 496 2454 Fragments arranged by size: 3401 2454 496 453 73 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2263 3608 1163 Size: 573 527 1345 4432 Fragments arranged by size: 4432 1345 573 527 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2660 3004 3040 4327 5088 6116 Size: 275 186 659 344 36 1287 761 1028 Cuts at: 6116 1540 Size: 2301 Fragments arranged by size: 2301 1287 1028 761 659 344 275 186 36 BsiHKAI G_wGCw'C Cuts at: 3567 4938 5023 6094 3567 Size: 1371 85 1071 4350 Fragments arranged by size: 4350 1371 1071 85 BsmI GAATG_Cn' Cuts at: 4388 4481 4388 Size: 93 6784 BsmBI CGTCTCn'nnnn_ Cuts at: 2826 3213 2826 Size: 387 6490 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2513 2793 3271 3567 3659 4013 4196 Size: 399 723 280 478 296 92 354 183 Cuts at: 4196 4938 5023 6094 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 723 478 399 354 296 280 183 92 85 BspEI T'CCGG_A Cuts at: 2845 3466 6540 2845 Size: 621 3074 3182 Fragments arranged by size: 3182 3074 621 BspHI T'CATG_A Cuts at: 4766 6496 4766 Size: 1730 5147 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2059 2422 4122 4232 844 Size: 1215 363 1700 110 3489 Fragments arranged by size: 3489 1700 1215 363 110 BsrBI CCG'CTC Cuts at: 1394 1823 2226 4764 1394 Size: 429 403 2538 3507 Fragments arranged by size: 3507 2538 429 403 BsrDI GCAATG_nn' Cuts at: 65 1248 4135 5372 5546 65 Size: 1183 2887 1237 174 1396 Fragments arranged by size: 2887 1396 1237 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2144 2684 2958 3969 5521 5708 1700 Size: 110 334 540 274 1011 1552 187 2869 Fragments arranged by size: 2869 1552 1011 540 334 274 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6877 BssHII G'CGCG_C Cuts at: 1925 2178 2229 2231 2575 1925 Size: 253 51 2 344 6227 Fragments arranged by size: 6227 344 253 51 2 BssSI C'ACGA_G Cuts at: 2618 4937 5949 2618 Size: 2319 1012 3546 Fragments arranged by size: 3546 2319 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2047 794 Size: 1253 5624 BstBI TT'CG_AA Cuts at: 5681 5681 Size: 6877 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6877 BstXI CCAn_nnnn'nTGG Cuts at: 3559 3559 Size: 6877 BstYI r'GATC_y Cuts at: 1972 2694 3042 4207 4574 4959 4976 6428 6514 Size: 722 348 1165 367 385 17 1452 86 Cuts at: 6514 6526 6872 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1165 722 385 367 348 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2250 3252 513 Size: 552 1185 1002 4138 Fragments arranged by size: 4138 1185 1002 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2661 2835 2955 3024 3120 487 Size: 683 56 1435 174 120 69 96 4244 Fragments arranged by size: 4244 1435 683 174 120 96 69 56 BtsI GCAGTG_nn' Cuts at: 2173 2275 3627 4393 5187 5207 2173 Size: 102 1352 766 794 20 3843 Fragments arranged by size: 3843 1352 794 766 102 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6877 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6842 DraI TTT'AAA Cuts at: 4181 4181 Size: 6877 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5884 809 Size: 507 4568 1802 Fragments arranged by size: 4568 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3001 3292 3376 Size: 54 1425 50 275 18 1171 291 84 Cuts at: 3376 3795 4023 4063 4324 5213 6842 8 Size: 419 228 40 261 889 1629 43 Fragments arranged by size: 1629 1425 1171 889 419 291 275 261 228 84 54 50 43 40 18 EagI C'GGCC_G Cuts at: 1537 1812 3001 4324 1537 Size: 275 1189 1323 4090 Fragments arranged by size: 4090 1323 1189 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3137 4807 1353 Size: 1784 1670 3423 Fragments arranged by size: 3423 1784 1670 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3176 5464 5838 5984 387 Size: 1064 138 1587 2288 374 146 1280 Fragments arranged by size: 2288 1587 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3481 3498 3709 4940 5537 6324 6592 Size: 393 1849 17 211 1231 597 787 268 Cuts at: 6592 1239 Size: 1524 Fragments arranged by size: 1849 1524 1231 787 597 393 268 211 17 EcoO109I rG'GnC_Cy Cuts at: 2866 3501 3839 3913 4009 4010 2866 Size: 635 338 74 96 1 5733 Fragments arranged by size: 5733 635 338 96 74 1 EcoRI G'AATT_C Cuts at: 4186 4564 4186 Size: 378 6499 FspI TGC'GCA Cuts at: 5383 5383 Size: 6877 HaeII r_GCGC'y Cuts at: 1678 2039 2587 2761 6024 1678 Size: 361 548 174 3263 2531 Fragments arranged by size: 3263 2531 548 361 174 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2543 2575 3872 3904 3929 Size: 32 135 32 971 32 1297 32 25 Cuts at: 3929 3961 5518 5550 5592 5624 1373 Size: 32 1557 32 42 32 2626 Fragments arranged by size: 2626 1557 1297 971 135 42 32 32 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 669 1657 1938 2290 4221 4467 669 Size: 988 281 352 1931 246 3079 Fragments arranged by size: 3079 1931 988 352 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6877 HpaI GTT'AAC Cuts at: 4467 4467 Size: 6877 KpnI G_GTAC'C Cuts at: 3077 4202 3077 Size: 1125 5752 MfeI C'AATT_G Cuts at: 4476 4476 Size: 6877 MluI A'CGCG_T Cuts at: 2115 6753 2115 Size: 4638 2239 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3835 3911 5991 6175 1305 Size: 2530 76 2080 184 2007 Fragments arranged by size: 2530 2080 2007 184 76 MscI TGG'CCA Cuts at: 10 64 1489 1832 3294 10 Size: 54 1425 343 1462 3593 Fragments arranged by size: 3593 1462 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2102 2622 3992 4052 4835 5194 5353 Size: 648 936 520 1370 60 783 359 159 Cuts at: 5353 518 Size: 2042 Fragments arranged by size: 2042 1370 936 783 648 520 359 159 60 MspA1I CmG'CkG Cuts at: 1606 2252 2328 2355 2382 2572 2893 3853 4125 Size: 646 76 27 27 190 321 960 272 Cuts at: 4125 4165 4293 4970 6118 6363 1606 Size: 40 128 677 1148 245 2120 Fragments arranged by size: 2120 1148 960 677 646 321 272 245 190 128 76 40 27 27 NaeI GCC'GGC Cuts at: 2686 2686 Size: 6877 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6325 NdeI CA'TA_TG Cuts at: 387 387 Size: 6877 NgoMIV G'CCGG_C Cuts at: 2684 2684 Size: 6877 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6877 NotI GC'GGCC_GC Cuts at: 4324 4324 Size: 6877 NruI TCG'CGA Cuts at: 2709 2709 Size: 6877 NspI r_CATG'y Cuts at: 1132 1229 2627 4235 5780 1132 Size: 97 1398 1608 1545 2229 Fragments arranged by size: 2229 1608 1545 1398 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6877 PciI A'CATG_T Cuts at: 5776 5776 Size: 6877 PflMI CCAn_nnn'nTGG Cuts at: 1108 2401 1108 Size: 1293 5584 PfoI T'CCnGG_A Cuts at: 2730 2730 Size: 6877 PmeI GTTT'AAAC Cuts at: 4181 4181 Size: 6877 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2069 2101 3644 3676 4946 4978 6459 6491 2069 Size: 32 1543 32 1270 32 1481 32 2455 Fragments arranged by size: 2455 1543 1481 1270 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2866 3913 2866 Size: 1047 5830 PshAI GACnn'nnGTC Cuts at: 1613 3132 3246 3462 1613 Size: 1519 114 216 5028 Fragments arranged by size: 5028 1519 216 114 PsiI TTA'TAA Cuts at: 4447 4447 Size: 6877 PspOMI G'GGCC_C Cuts at: 4009 4009 Size: 6877 PstI C_TGCA'G Cuts at: 830 2022 2043 3966 4229 830 Size: 1192 21 1923 263 3478 Fragments arranged by size: 3478 1923 1192 263 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6877 PvuII CAG'CTG Cuts at: 3853 4165 3853 Size: 312 6565 SacII CC_GC'GG Cuts at: 2253 2253 Size: 6877 SalI G'TCGA_C Cuts at: 1936 4219 1936 Size: 2283 4594 SanDI GG'GwC_CC Cuts at: 2866 2866 Size: 6877 SapI GCTCTTCn'nnn_ Cuts at: 3137 3137 Size: 6877 SbfI CC_TGCA'GG Cuts at: 4229 4229 Size: 6877 ScaI AGT'ACT Cuts at: 5125 6837 5125 Size: 1712 5165 SfcI C'TryA_G Cuts at: 826 1045 2018 2039 3962 4225 5360 6041 6232 Size: 219 973 21 1923 263 1135 681 191 Cuts at: 6232 826 Size: 1471 Fragments arranged by size: 1923 1471 1135 973 681 263 219 191 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6877 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6877 SmaI CCC'GGG Cuts at: 1872 2346 2507 3844 4204 1872 Size: 474 161 1337 360 4545 Fragments arranged by size: 4545 1337 474 360 161 SmlI C'TyrA_G Cuts at: 820 1017 1943 3815 4981 5882 6144 820 Size: 197 926 1872 1166 901 262 1553 Fragments arranged by size: 1872 1553 1166 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4668 493 Size: 4175 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6877 SphI G_CATG'C Cuts at: 1132 2627 4235 1132 Size: 1495 1608 3774 Fragments arranged by size: 3774 1608 1495 SspI AAT'ATT Cuts at: 5 52 4801 5 Size: 47 4749 2081 Fragments arranged by size: 4749 2081 47 StuI AGG'CCT Cuts at: 1517 3612 1517 Size: 2095 4782 StyI C'CwwG_G Cuts at: 513 1065 2418 3068 4316 513 Size: 552 1353 650 1248 3074 Fragments arranged by size: 3074 1353 1248 650 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2405 3547 4904 4921 5074 5259 5704 Size: 1255 825 1142 1357 17 153 185 445 Cuts at: 5704 325 Size: 1498 Fragments arranged by size: 1498 1357 1255 1142 825 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5123 6835 96 Size: 274 80 33 51 1443 3146 1712 138 Fragments arranged by size: 3146 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2686 2718 2747 2779 6837 6869 2686 Size: 32 29 32 4058 32 2694 Fragments arranged by size: 4058 2694 32 32 32 29 Tth111I GACn'n_nGTC Cuts at: 1316 2633 2864 3332 1316 Size: 1317 231 468 4861 Fragments arranged by size: 4861 1317 468 231 XbaI T'CTAG_A Cuts at: 4213 4213 Size: 6877 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3733 3733 Size: 6877 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6877 XmaI C'CCGG_G Cuts at: 1870 2344 2505 3842 4202 1870 Size: 474 161 1337 360 4545 Fragments arranged by size: 4545 1337 474 360 161 XmnI GAAnn'nnTTC Cuts at: 3782 5006 3782 Size: 1224 5653 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2287 4685 276 Size: 53 83 186 936 753 2398 2468 Fragments arranged by size: 2468 2398 936 753 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NsiI PacI PmlI PspXI PsrI RsrII SacI SexAI SfiI SfoI SrfI SwaI