################################## # NO CUT ENZYMES ( 184 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BclI BcnI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr13I ClaI CpoI DraI DraII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MseI MunI MvnI NarI NciI NcoI NdeI NheI NlaIII NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AcsI 86 AAATTC [GA]^AATT[CT] AlwNI 121 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 86 AAATTC [GA]^AATT[CT] BanII 52 GAGCCC G[GA]GC[CT]^C BbsI 15 GAAGAC GAAGAC(2/6) BbvI 120 GCAGC GCAGC(8/12) BciVI 104 GTATCC GTATCC(6/5) BglII 5 AGATCT A^GATCT BmyI 52 GAGCCC G[AGT]GC[ACT]^C BpmI 148 CTGGAG CTGGAG(16/14) BpuAI 15 GAAGAC GAAGAC(2/6) BsgI 199 CTGCAC GTGCAG(16/14) BsiYI 198 CCTGCACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 198 CCTGCACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 365 GTCCC GGGAC(10/14) Bsp1286I 52 GAGCCC G[AGT]GC[ACT]^C BspMI 359 ACCTGC ACCTGC(4/8) BsrFI 136 GCCGGC [GA]^CCGG[CT] BssKI 204 CCTGG ^CC[ACGT]GG BssSI 314 CTCGTG CACGAG(-5/-1) BstNI 204 CCTGG CC^[AT]GG BstYI 5 AGATCT [GA]^GATC[CT] CfoI 213 GCGC GCG^C Cfr10I 136 GCCGGC [GA]^CCGG[CT] DdeI 168 CTCAG C^T[ACGT]AG DraIII 202 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 156 TGGCCG [CT]^GGCC[GA] EcoRII 204 CCTGG ^CC[AT]GG HaeIII 157 GGCC GG^CC HapII 137 CCGG C^CGG HhaI 213 GCGC GCG^C HinP1I 213 GCGC G^CGC HincII 322 GTCAAC GT[CT]^[GA]AC HindII 322 GTCAAC GT[CT]^[GA]AC HinfI 190 GAGTC G^A[ACGT]TC HpaII 137 CCGG C^CGG MflI 5 AGATCT [GA]^GATC[CT] MslI 310 CACCCTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 145 CAGCTG C[AC]G^C[GT]G MspI 137 CCGG C^CGG MvaI 204 CCTGG CC^[AT]GG NaeI 136 GCCGGC GCC^GGC NgoMIV 136 GCCGGC G^CCGGC PleI 190 GAGTC GAGTC(4/5) PvuII 145 CAGCTG CAG^CTG ScrFI 204 CCTGG CC^[ACGT]GG SexAI 203 ACCTGGT A^CC[AT]GGT SmlI 43 CTTGAG C^T[CT][GA]AG TaqI 50 TCGA T^CGA TseI 120 GCAGC G^C[AT]GC Tsp509I 87 AATT ^AATT TthHB8I 50 TCGA T^CGA XcmI 194 CCACCCTGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 5 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AluI 146 AGCT AG^CT 251 AGCT BsrI 25 ACTGG ACTGG(1/-1) 56 CCAGT BstF5I 180 CATCC GGATG(2/0) 153 GGATG DpnII 6 GATC ^GATC 282 GATC FokI 180 CATCC GGATG(9/13) 153 GGATG HgaI 123 GCGTC GACGC(5/10) 363 GCGTC HphI 38 TCACC GGTGA(8/7) 309 TCACC MaeIII 59 GTGAC ^GT[ACGT]AC 347 GTCAC MboI 6 GATC ^GATC 282 GATC MwoI 287 GCCGCAAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 296 GCTATGCTCGC NdeII 6 GATC ^GATC 282 GATC Sau3AI 6 GATC ^GATC 282 GATC Tsp45I 59 GTGAC ^GT[GC]AC 347 GTCAC TspRI 24 CACTG CA[GC]TG[ACGT][ACGT]^ 57 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 6 enzymes) # ######################################### AciI Cac8I Fnu4HI ItaI MboII MnlI