Restriction map for
pFN21AE3122
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BalI
BbrPI BbvCI BclI BfaI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaI BseRI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrFI BsrGI Bst1107I BstAPI BstEII
BstF5I BstPI BstXI BstZ17I Bsu36I
Cfr10I ClaI DraI DraIII DrdI
Eam1105I EarI Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FokI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MluNI MscI
MseI MunI NaeI NdeI NgoMIV
NheI NruI NsiI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsaI SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tsp509I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 257 TCCGGA T^CCGGA
ApaI 616 GGGCCC GGGCC^C
BamHI 286 GGATCC G^GATCC
BcgI 511 GCAGAATCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 260 GGATAC GTATCC(6/5)
BlpI 432 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 432 GCTCAGC GC^T[ACGT]AGC
BsaWI 257 TCCGGA [AT]^CCGG[AT]
BseAI 257 TCCGGA T^CCGGA
BsiEI 553 CGGCCG CG[GA][CT]^CG
BsmAI 90 GTCTC GTCTC(1/5)
BspEI 257 TCCGGA T^CCGGA
BsrBI 610 CCGCTC CCGCTC(-3/-3)
BsrI 738 CCAGT ACTGG(1/-1)
BssHII 589 GCGCGC G^CGCGC
BssSI 63 CTCGTG CACGAG(-5/-1)
BstBI 225 TTCGAA TT^CGAA
BstYI 286 GGATCC [GA]^GATC[CT]
CelII 432 GCTCAGC GC^T[ACGT]AGC
CpoI 644 CGGTCCG CG^G[AT]CCG
EagI 553 CGGCCG C^GGCCG
EclXI 553 CGGCCG C^GGCCG
Eco52I 553 CGGCCG C^GGCCG
EcoNI 327 CCTTCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 842 CCATGG C^C[AT][AT]GG
MaeII 407 ACGT A^CGT
MflI 286 GGATCC [GA]^GATC[CT]
MroI 257 TCCGGA T^CCGGA
MslI 867 CACCGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 535 CAGCTG C[AC]G^C[GT]G
NcoI 842 CCATGG C^CATGG
NotI 552 GCGGCCGC GC^GGCCGC
NspI 755 ACATGC [GA]CATG^[CT]
NspV 225 TTCGAA TT^CGAA
PflMI 501 CCAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 148 GACTC GAGTC(4/5)
PshAI 601 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 251 CTGCAG CTGCA^G
PvuII 535 CAGCTG CAG^CTG
RsrII 644 CGGTCCG CG^G[AT]CCG
SfcI 251 CTGCAG C^T[GA][CT]AG
SfuI 225 TTCGAA TT^CGAA
StuI 347 AGGCCT AGG^CCT
StyI 842 CCATGG C^C[AT][AT]GG
TaqI 226 TCGA T^CGA
TthHB8I 226 TCGA T^CGA
Van91I 501 CCAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 286 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 287 GATCC GGATC(4/5)
286 GGATC
AspHI 58 GTGCTC G[AT]GC[AT]^C
847 GTGCTC
AspI 235 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
387 GACGGCGTC
BbsI 145 GAAGAC GAAGAC(2/6)
661 GAAGAC
BpuAI 145 GAAGAC GAAGAC(2/6)
661 GAAGAC
BsiHKAI 58 GTGCTC G[AT]GC[AT]^C
847 GTGCTC
DpnII 287 GATC ^GATC
768 GATC
DraII 123 GGGACCC [GA]G^G[ACGT]CC[CT]
182 AGGACCT
DsaI 694 CCACGG C^C[GA][CT]GG
842 CCATGG
EcoO109I 123 GGGACCC [GA]G^G[ACGT]CC[CT]
182 AGGACCT
HphI 24 GGTGA GGTGA(8/7)
360 TCACC
MaeIII 409 GTGAC ^GT[ACGT]AC
709 GTGAC
MboI 287 GATC ^GATC
768 GATC
NdeII 287 GATC ^GATC
768 GATC
PflFI 235 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
387 GACGGCGTC
PpuMI 123 GGGACCC [GA]G^G[AT]CC[CT]
182 AGGACCT
Sau3AI 287 GATC ^GATC
768 GATC
SfaNI 39 GATGC GCATC(5/9)
719 GCATC
Tsp45I 409 GTGAC ^GT[GC]AC
709 GTGAC
TspRI 604 CACTG CA[GC]TG[ACGT][ACGT]^
916 CACTG
Tth111I 235 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
387 GACGGCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BmyI BpmI BsaHI BsaJI
BsgI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI EaeI
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII ItaI KasI MboII
MnlI MspI MvaI MvnI MwoI
NarI NciI NlaIII NlaIV Sau96I
ScrFI SfoI SmaI TfiI TseI
XmaCI XmaI