(Circular) (Six-base) MAPSORT of: pFN21AE3122.seq Check: 2722 from: 1 to: 5629 pFN21AE3122 5629 bp With 182 enzymes: SgfI * March 28, 2012 13:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2313 2319 2481 2313 Size: 6 162 5461 Fragments arranged by size: 5461 162 6 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3439 278 Size: 53 83 186 936 1903 2468 Fragments arranged by size: 2468 1903 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2972 1937 Size: 1035 4594 Acc65I G'GTAC_C Cuts at: 2950 2950 Size: 5629 AclI AA'CG_TT Cuts at: 1563 3756 4129 1563 Size: 2193 373 3063 Fragments arranged by size: 3063 2193 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2816 3692 5076 5344 1632 Size: 1184 876 1384 268 1917 Fragments arranged by size: 1917 1384 1184 876 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5432 AflIII A'CryG_T Cuts at: 1203 4528 5505 1203 Size: 3325 977 1327 Fragments arranged by size: 3325 1327 977 AgeI A'CCGG_T Cuts at: 4460 4460 Size: 5629 AhdI GACnn_n'nnGTC Cuts at: 4358 4358 Size: 5629 AleI CACnn'nnGTG Cuts at: 1166 2876 1166 Size: 1710 3919 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5629 AlwNI CAG_nnn'CTG Cuts at: 3372 4944 3372 Size: 1572 4057 ApaI G_GGCC'C Cuts at: 2625 2625 Size: 5629 ApaLI G'TGCA_C Cuts at: 3686 4842 3686 Size: 1156 4473 ApoI r'AATT_y Cuts at: 1493 2938 3164 3316 5425 1493 Size: 1445 226 152 2109 1697 Fragments arranged by size: 2109 1697 1445 226 152 AseI AT'TA_AT Cuts at: 160 1032 4183 160 Size: 872 3151 1606 Fragments arranged by size: 3151 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 AvaI C'yCGr_G Cuts at: 1870 1943 2009 2125 2322 2336 2348 2618 2624 Size: 73 66 116 197 14 12 270 6 Cuts at: 2624 2737 2954 1870 Size: 113 217 4545 Fragments arranged by size: 4545 270 217 197 116 113 73 66 14 12 6 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2299 2332 2299 Size: 33 5596 BamHI G'GATC_C Cuts at: 2291 2959 3326 2291 Size: 668 367 4594 Fragments arranged by size: 4594 668 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2087 2458 2577 2809 2950 Size: 325 217 627 300 371 119 232 141 Cuts at: 2950 4405 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 371 325 300 232 217 141 119 BanII G_rGCy'C Cuts at: 1391 2346 2374 2625 2948 1391 Size: 955 28 251 323 4072 Fragments arranged by size: 4072 955 323 251 28 BbeI G_GCGC'C Cuts at: 2091 2462 2581 2091 Size: 371 119 5139 Fragments arranged by size: 5139 371 119 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2157 2673 928 Size: 971 258 516 3884 Fragments arranged by size: 3884 971 516 258 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2505 2539 3820 3854 1903 Size: 34 50 34 484 34 1281 34 3678 Fragments arranged by size: 3678 1281 484 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2259 3521 4731 2259 Size: 1262 1210 3157 Fragments arranged by size: 3157 1262 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5629 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4240 136 Size: 107 122 71 631 605 2568 1525 Fragments arranged by size: 2568 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5624 5624 Size: 5629 BlpI GC'TnA_GC Cuts at: 2438 3046 2438 Size: 608 5021 Bme1580I G_kGCm'C Cuts at: 1790 2625 2738 2832 3690 4846 1790 Size: 835 113 94 858 1156 2573 Fragments arranged by size: 2573 1156 858 835 113 94 BmgBI CAC'GTC Cuts at: 1318 2413 1318 Size: 1095 4534 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3360 4318 442 Size: 416 53 83 399 24 1943 958 1753 Fragments arranged by size: 1943 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5629 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2140 2564 2870 4289 1239 Size: 901 424 306 1419 2579 Fragments arranged by size: 2579 1419 901 424 306 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3754 4619 4917 3754 Size: 865 298 4466 Fragments arranged by size: 4466 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4292 882 Size: 617 2793 2219 Fragments arranged by size: 2793 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3420 493 Size: 711 2216 2702 Fragments arranged by size: 2702 2216 711 BsaBI GATnn'nnATC Cuts at: 5623 5623 Size: 5629 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2088 2396 2431 2459 Size: 53 83 186 936 555 308 35 28 Cuts at: 2459 2578 3436 3818 4453 275 Size: 119 858 382 635 1451 Fragments arranged by size: 1451 936 858 635 555 382 308 186 119 83 53 35 28 BsaWI w'CCGG_w Cuts at: 2262 4062 4460 4734 4881 5292 2262 Size: 1800 398 274 147 411 2599 Fragments arranged by size: 2599 1800 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5404 Fragments arranged by size: 5404 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5629 BseYI C'CCAG_C Cuts at: 2220 2287 2538 2676 4832 5328 2220 Size: 67 251 138 2156 496 2521 Fragments arranged by size: 2521 2156 496 251 138 67 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2291 2453 2627 2771 1163 Size: 573 555 162 174 144 4021 Fragments arranged by size: 4021 573 555 174 162 144 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2561 3079 3840 4868 1540 Size: 275 186 560 518 761 1028 2301 Fragments arranged by size: 2301 1028 761 560 518 275 186 BsiHKAI G_wGCw'C Cuts at: 2067 2856 3690 3775 4846 2067 Size: 789 834 85 1071 2850 Fragments arranged by size: 2850 1071 834 789 85 BsmI GAATG_Cn' Cuts at: 3140 3233 3140 Size: 93 5536 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2346 2374 2625 2738 2832 2856 Size: 399 277 279 28 251 113 94 24 Cuts at: 2856 2948 3690 3775 4846 1391 Size: 92 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 279 277 251 113 94 92 85 28 24 BspEI T'CCGG_A Cuts at: 2262 5292 2262 Size: 3030 2599 BspHI T'CATG_A Cuts at: 3518 5248 3518 Size: 1730 3899 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2199 2313 2319 2481 2984 844 Size: 1355 114 6 162 503 3489 Fragments arranged by size: 3489 1355 503 162 114 6 BsrBI CCG'CTC Cuts at: 1394 1823 2617 3516 1394 Size: 429 794 899 3507 Fragments arranged by size: 3507 899 794 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4124 4298 65 Size: 1183 2876 174 1396 Fragments arranged by size: 2876 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4273 4460 1700 Size: 110 2463 187 2869 Fragments arranged by size: 2869 2463 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5629 BssHII G'CGCG_C Cuts at: 1925 2594 1925 Size: 669 4960 BssSI C'ACGA_G Cuts at: 2068 3689 4701 2068 Size: 1621 1012 2996 Fragments arranged by size: 2996 1621 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5629 BstBI TT'CG_AA Cuts at: 2231 4433 2231 Size: 2202 3427 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5629 BstYI r'GATC_y Cuts at: 1972 2291 2959 3326 3711 3728 5180 5266 5278 Size: 319 668 367 385 17 1452 86 12 Cuts at: 5278 5624 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 668 385 367 346 319 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2699 2847 513 Size: 552 1634 148 3295 Fragments arranged by size: 3295 1634 552 148 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4890 Fragments arranged by size: 4890 683 56 BtsI GCAGTG_nn' Cuts at: 2920 3145 3939 3959 2920 Size: 225 794 20 4590 Fragments arranged by size: 4590 794 225 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5629 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5594 DraI TTT'AAA Cuts at: 2933 2933 Size: 5629 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4636 809 Size: 507 3320 1802 Fragments arranged by size: 3320 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2100 2299 2558 Size: 54 1425 50 275 18 270 199 259 Cuts at: 2558 3076 3965 5594 8 Size: 518 889 1629 43 Fragments arranged by size: 1629 1425 889 518 275 270 259 199 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2558 3076 1537 Size: 275 746 518 4090 Fragments arranged by size: 4090 746 518 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3559 1353 Size: 2206 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4216 4590 4736 387 Size: 1064 138 2627 374 146 1280 Fragments arranged by size: 2627 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2140 2564 2816 2870 3692 4289 5076 Size: 393 508 424 252 54 822 597 787 Cuts at: 5076 5344 1239 Size: 268 1524 Fragments arranged by size: 1524 822 787 597 508 424 393 268 252 54 EcoNI CCTnn'n_nnAGG Cuts at: 2336 2336 Size: 5629 EcoO109I rG'GnC_Cy Cuts at: 2129 2188 2129 Size: 59 5570 EcoRI G'AATT_C Cuts at: 2938 3316 2938 Size: 378 5251 FspI TGC'GCA Cuts at: 4135 4135 Size: 5629 HaeII r_GCGC'y Cuts at: 1678 2091 2462 2581 2819 4776 1678 Size: 413 371 119 238 1957 2531 Fragments arranged by size: 2531 1957 413 371 238 119 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2407 2439 4270 4302 4344 Size: 32 135 32 835 32 1831 32 42 Cuts at: 4344 4376 1373 Size: 32 2626 Fragments arranged by size: 2626 1831 835 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2973 3219 669 Size: 988 281 1035 246 3079 Fragments arranged by size: 3079 1035 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5629 HpaI GTT'AAC Cuts at: 3219 3219 Size: 5629 KasI G'GCGC_C Cuts at: 2087 2458 2577 2087 Size: 371 119 5139 Fragments arranged by size: 5139 371 119 KpnI G_GTAC'C Cuts at: 2954 2954 Size: 5629 MfeI C'AATT_G Cuts at: 3228 3228 Size: 5629 MluI A'CGCG_T Cuts at: 5505 5505 Size: 5629 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2481 4743 4927 1305 Size: 1176 2262 184 2007 Fragments arranged by size: 2262 2007 1176 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3807 Fragments arranged by size: 3807 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2876 3587 3946 4105 518 Size: 648 1710 711 359 159 2042 Fragments arranged by size: 2042 1710 711 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2542 3045 3722 4870 5115 1606 Size: 936 503 677 1148 245 2120 Fragments arranged by size: 2120 1148 936 677 503 245 NarI GG'CG_CC Cuts at: 2088 2459 2578 2088 Size: 371 119 5139 Fragments arranged by size: 5139 371 119 NcoI C'CATG_G Cuts at: 513 1065 2847 513 Size: 552 1782 3295 Fragments arranged by size: 3295 1782 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5629 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5629 NotI GC'GGCC_GC Cuts at: 2558 3076 2558 Size: 518 5111 NspI r_CATG'y Cuts at: 1132 1229 2764 2987 4532 1132 Size: 97 1535 223 1545 2229 Fragments arranged by size: 2229 1545 1535 223 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5629 PciI A'CATG_T Cuts at: 4528 4528 Size: 5629 PflMI CCAn_nnn'nTGG Cuts at: 1108 2512 1108 Size: 1404 4225 PmeI GTTT'AAAC Cuts at: 2933 2933 Size: 5629 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3698 3730 5211 5243 3698 Size: 32 1481 32 4084 Fragments arranged by size: 4084 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2129 2188 2129 Size: 59 5570 PshAI GACnn'nnGTC Cuts at: 1613 2610 1613 Size: 997 4632 PsiI TTA'TAA Cuts at: 3199 3199 Size: 5629 PspOMI G'GGCC_C Cuts at: 2621 2621 Size: 5629 PstI C_TGCA'G Cuts at: 830 2260 2981 830 Size: 1430 721 3478 Fragments arranged by size: 3478 1430 721 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5629 PvuII CAG'CTG Cuts at: 2542 2542 Size: 5629 RsrII CG'GwC_CG Cuts at: 2650 2650 Size: 5629 SalI G'TCGA_C Cuts at: 1936 2971 1936 Size: 1035 4594 SanDI GG'GwC_CC Cuts at: 2129 2129 Size: 5629 SbfI CC_TGCA'GG Cuts at: 2981 2981 Size: 5629 ScaI AGT'ACT Cuts at: 3877 5589 3877 Size: 1712 3917 SfcI C'TryA_G Cuts at: 826 1045 2256 2977 4112 4793 4984 826 Size: 219 1211 721 1135 681 191 1471 Fragments arranged by size: 1471 1211 1135 721 681 219 191 SfoI GGC'GCC Cuts at: 2089 2460 2579 2089 Size: 371 119 5139 Fragments arranged by size: 5139 371 119 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5629 SmaI CCC'GGG Cuts at: 1872 2011 2127 2626 2956 1872 Size: 139 116 499 330 4545 Fragments arranged by size: 4545 499 330 139 116 SmlI C'TyrA_G Cuts at: 820 1017 1943 3733 4634 4896 820 Size: 197 926 1790 901 262 1553 Fragments arranged by size: 1790 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3420 493 Size: 2927 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5629 SphI G_CATG'C Cuts at: 1132 2987 1132 Size: 1855 3774 SrfI GCCC'GGGC Cuts at: 2011 2626 2011 Size: 615 5014 SspI AAT'ATT Cuts at: 5 52 3553 5 Size: 47 3501 2081 Fragments arranged by size: 3501 2081 47 StuI AGG'CCT Cuts at: 1517 2354 1517 Size: 837 4792 StyI C'CwwG_G Cuts at: 513 1065 2847 3068 513 Size: 552 1782 221 3074 Fragments arranged by size: 3074 1782 552 221 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2677 2882 3656 3673 3826 4011 4456 Size: 1255 1097 205 774 17 153 185 445 Cuts at: 4456 325 Size: 1498 Fragments arranged by size: 1498 1255 1097 774 445 205 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3875 5587 96 Size: 274 80 33 51 1443 1898 1712 138 Fragments arranged by size: 1898 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5589 5621 5589 Size: 32 5597 Tth111I GACn'n_nGTC Cuts at: 1316 2243 2395 1316 Size: 927 152 4550 Fragments arranged by size: 4550 927 152 XbaI T'CTAG_A Cuts at: 2965 2965 Size: 5629 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5629 XmaI C'CCGG_G Cuts at: 1870 2009 2125 2624 2954 1870 Size: 139 116 499 330 4545 Fragments arranged by size: 4545 499 330 139 116 XmnI GAAnn'nnTTC Cuts at: 3758 3758 Size: 5629 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3437 276 Size: 53 83 186 936 1903 2468 Fragments arranged by size: 2468 1903 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI SacI SacII SapI SexAI SfiI SwaI XcmI