Restriction map for
pFN21AE3122
(Linear) (Six-base) MAPSORT of: KIEE3122.seq Check: 7240 from: 1 to: 924
KIEE3122.orf
With 182 enzymes: SgfI *
March 22, 2012 16:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 308 314 476 924
Size: 308 6 162 448
Fragments arranged by size:
448 308 162 6
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 811 924
Size: 811 113
AleI CACnn'nnGTG
Cuts at: 0 871 924
Size: 871 53
ApaI G_GGCC'C
Cuts at: 0 620 924
Size: 620 304
AvaI C'yCGr_G
Cuts at: 0 4 120 317 331 343 613 619 732
Size: 4 116 197 14 12 270 6 113
Cuts at: 732 924
Size: 192
Fragments arranged by size:
270 197 192 116 113 14 12 6
4
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 294 327 924
Size: 294 33 597
Fragments arranged by size:
597 294 33
BamHI G'GATC_C
Cuts at: 0 286 924
Size: 286 638
BanI G'GyrC_C
Cuts at: 0 82 453 572 804 924
Size: 82 371 119 232 120
Fragments arranged by size:
371 232 120 119 82
BanII G_rGCy'C
Cuts at: 0 341 369 620 924
Size: 341 28 251 304
Fragments arranged by size:
341 304 251 28
BbeI G_GCGC'C
Cuts at: 0 86 457 576 924
Size: 86 371 119 348
Fragments arranged by size:
371 348 119 86
BbsI GAAGACnn'nnnn_
Cuts at: 0 152 668 924
Size: 152 516 256
Fragments arranged by size:
516 256 152
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 500 534 924
Size: 500 34 390
Fragments arranged by size:
500 390 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 254 924
Size: 254 670
BlpI GC'TnA_GC
Cuts at: 0 433 924
Size: 433 491
Bme1580I G_kGCm'C
Cuts at: 0 620 733 827 924
Size: 620 113 94 97
Fragments arranged by size:
620 113 97 94
BmgBI CAC'GTC
Cuts at: 0 408 924
Size: 408 516
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 559 865 924
Size: 135 424 306 59
Fragments arranged by size:
424 306 135 59
BsaHI Gr'CG_yC
Cuts at: 0 83 391 426 454 573 924
Size: 83 308 35 28 119 351
Fragments arranged by size:
351 308 119 83 35 28
BsaWI w'CCGG_w
Cuts at: 0 257 924
Size: 257 667
BseYI C'CCAG_C
Cuts at: 0 215 282 533 671 924
Size: 215 67 251 138 253
Fragments arranged by size:
253 251 215 138 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 448 622 766 924
Size: 286 162 174 144 158
Fragments arranged by size:
286 174 162 158 144
BsiEI CG_ry'CG
Cuts at: 0 556 924
Size: 556 368
BsiHKAI G_wGCw'C
Cuts at: 0 62 851 924
Size: 62 789 73
Fragments arranged by size:
789 73 62
Bsp1286I G_dGCh'C
Cuts at: 0 62 341 369 620 733 827 851 924
Size: 62 279 28 251 113 94 24 73
Fragments arranged by size:
279 251 113 94 73 62 28 24
BspEI T'CCGG_A
Cuts at: 0 257 924
Size: 257 667
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 194 308 314 476 924
Size: 194 114 6 162 448
Fragments arranged by size:
448 194 162 114 6
BsrBI CCG'CTC
Cuts at: 0 612 924
Size: 612 312
BssHII G'CGCG_C
Cuts at: 0 589 924
Size: 589 335
BssSI C'ACGA_G
Cuts at: 0 63 924
Size: 63 861
BstBI TT'CG_AA
Cuts at: 0 226 924
Size: 226 698
BstYI r'GATC_y
Cuts at: 0 286 924
Size: 286 638
BtgI C'CryG_G
Cuts at: 0 694 842 924
Size: 694 148 82
Fragments arranged by size:
694 148 82
BtsI GCAGTG_nn'
Cuts at: 0 915 924
Size: 915 9
EaeI y'GGCC_r
Cuts at: 0 95 294 553 924
Size: 95 199 259 371
Fragments arranged by size:
371 259 199 95
EagI C'GGCC_G
Cuts at: 0 553 924
Size: 553 371
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 559 811 865 924
Size: 135 424 252 54 59
Fragments arranged by size:
424 252 135 59 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 331 924
Size: 331 593
EcoO109I rG'GnC_Cy
Cuts at: 0 124 183 924
Size: 124 59 741
Fragments arranged by size:
741 124 59
HaeII r_GCGC'y
Cuts at: 0 86 457 576 814 924
Size: 86 371 119 238 110
Fragments arranged by size:
371 238 119 110 86
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 402 434 924
Size: 402 32 490
Fragments arranged by size:
490 402 32
KasI G'GCGC_C
Cuts at: 0 82 453 572 924
Size: 82 371 119 352
Fragments arranged by size:
371 352 119 82
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 924
Size: 476 448
MslI CAynn'nnrTG
Cuts at: 0 871 924
Size: 871 53
MspA1I CmG'CkG
Cuts at: 0 537 924
Size: 537 387
NarI GG'CG_CC
Cuts at: 0 83 454 573 924
Size: 83 371 119 351
Fragments arranged by size:
371 351 119 83
NcoI C'CATG_G
Cuts at: 0 842 924
Size: 842 82
NotI GC'GGCC_GC
Cuts at: 0 553 924
Size: 553 371
NspI r_CATG'y
Cuts at: 0 759 924
Size: 759 165
PflMI CCAn_nnn'nTGG
Cuts at: 0 507 924
Size: 507 417
PpuMI rG'GwC_Cy
Cuts at: 0 124 183 924
Size: 124 59 741
Fragments arranged by size:
741 124 59
PshAI GACnn'nnGTC
Cuts at: 0 605 924
Size: 605 319
PspOMI G'GGCC_C
Cuts at: 0 616 924
Size: 616 308
PstI C_TGCA'G
Cuts at: 0 255 924
Size: 255 669
PvuII CAG'CTG
Cuts at: 0 537 924
Size: 537 387
RsrII CG'GwC_CG
Cuts at: 0 645 924
Size: 645 279
SanDI GG'GwC_CC
Cuts at: 0 124 924
Size: 124 800
SfcI C'TryA_G
Cuts at: 0 251 924
Size: 251 673
SfoI GGC'GCC
Cuts at: 0 84 455 574 924
Size: 84 371 119 350
Fragments arranged by size:
371 350 119 84
SmaI CCC'GGG
Cuts at: 0 6 122 621 924
Size: 6 116 499 303
Fragments arranged by size:
499 303 116 6
SrfI GCCC'GGGC
Cuts at: 0 6 621 924
Size: 6 615 303
Fragments arranged by size:
615 303 6
StuI AGG'CCT
Cuts at: 0 349 924
Size: 349 575
StyI C'CwwG_G
Cuts at: 0 842 924
Size: 842 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 672 877 924
Size: 672 205 47
Fragments arranged by size:
672 205 47
Tth111I GACn'n_nGTC
Cuts at: 0 238 390 924
Size: 238 152 534
Fragments arranged by size:
534 238 152
XmaI C'CCGG_G
Cuts at: 0 4 120 619 924
Size: 4 116 499 305
Fragments arranged by size:
499 305 116 4
Enzymes that do cut:
AarI AcuI AleI ApaI AvaI BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BlpI Bme1580I BmgBI
BpmI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspMI BsrBI BssHII BssSI BstBI BstYI BtgI
BtsI EaeI EagI Eco57MI EcoNI EcoO109I HaeII Hin4I
KasI MmeI MslI MspA1I NarI NcoI NotI NspI
PflMI PpuMI PshAI PspOMI PstI PvuII RsrII SanDI
SfcI SfoI SmaI SrfI StuI StyI TaqII Tth111I
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BbvCI BclI BfrBI BglI BglII BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI
BseRI BsiWI BsmI BsmBI BspHI BsrDI BsrFI BsrGI
BstAPI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EarI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MscI NaeI NdeI NgoMIV NheI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PsiI PspXI PsrI PvuI SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI
SpeI SphI SspI SwaI TatI TstI XbaI XcmI
XhoI XmnI ZraI