################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII AccI AclI AcsI AflII AgeI AocI ApoI AscI AseI AsnI BbrPI BcgI BciVI BclI BfrI BglII BlpI Bpu1102I BsaAI BsaBI BsgI BsiWI BsmI BspDI BspHI BssHII Bst1107I BstBI BstXI BstZ17I Bsu36I CelII ClaI DraI Eco81I EcoRI EcoT22I FbaI FseI HindIII HpaI MamI MfeI MluI MunI NdeI NheI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RcaI SalI SapI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI SspI SwaI Tsp509I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 1898 GGTACC G^GTACC AccIII 147 TCCGGA T^CCGGA AflIII 1499 ACATGT A^C[GA][CT]GT AhdI 286 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1624 GTGCAC G^TGCAC Aor51HI 131 AGCGCT AGC^GCT ApaI 278 GGGCCC GGGCC^C ApaLI 1624 GTGCAC G^TGCAC Asp718I 1898 GGTACC G^GTACC AspEI 286 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1463 TGCGCA TGC^GCA AvrII 531 CCTAGG C^CTAGG BbeI 110 GGCGCC GGCGC^C BlnI 531 CCTAGG C^CTAGG BsaWI 147 TCCGGA [AT]^CCGG[AT] BseAI 147 TCCGGA T^CCGGA Bsp1407I 1349 TGTACA T^GTACA BspEI 147 TCCGGA T^CCGGA BspLU11I 1499 ACATGT A^CATGT BsrGI 1349 TGTACA T^GTACA BssSI 264 CTCGTG CACGAG(-5/-1) BstAPI 1200 GCATGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 831 GGTGACC G^GT[ACGT]ACC BstPI 831 GGTGACC G^GT[ACGT]ACC DrdI 735 GACCCACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 286 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 131 AGCGCT AGC^GCT EcoNI 230 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 831 GGTGACC G^GT[ACGT]ACC EcoRV 1423 GATATC GAT^ATC FspI 1463 TGCGCA TGC^GCA HincII 732 GTTGAC GT[CT]^[GA]AC HindII 732 GTTGAC GT[CT]^[GA]AC KasI 110 GGCGCC G^GCGCC KpnI 1898 GGTACC GGTAC^C MroI 147 TCCGGA T^CCGGA MseI 1322 TTAA T^TAA NaeI 2092 GCCGGC GCC^GGC NarI 110 GGCGCC GG^CGCC NcoI 1538 CCATGG C^CATGG NgoMIV 2092 GCCGGC G^CCGGC NotI 59 GCGGCCGC GC^GGCCGC PaeR7I 1225 CTCGAG C^TCGAG PshAI 1741 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 196 GAGCTC GAGCT^C SbfI 233 CCTGCAGG CCTGCA^GG SfoI 110 GGCGCC GGC^GCC Sse8387I 233 CCTGCAGG CCTGCA^GG SspBI 1349 TGTACA T^GTACA StuI 2051 AGGCCT AGG^CCT Tru9I 1322 TTAA T^TAA XhoI 1225 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Asp700I 560 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1080 GAAGACATTC AspHI 196 GAGCTC G[AT]GC[AT]^C 1624 GTGCAC AspI 1454 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1840 GACCTGGTC AvaI 1225 CTCGAG C^[CT]CG[GA]G 1942 CCCGAG BamHI 144 GGATCC G^GATCC 716 GGATCC BbsI 973 GAAGAC GAAGAC(2/6) 1080 GAAGAC BpuAI 973 GAAGAC GAAGAC(2/6) 1080 GAAGAC BsiHKAI 196 GAGCTC G[AT]GC[AT]^C 1624 GTGCAC BsmBI 622 GAGACG CGTCTC(1/5) 661 GAGACG BsoBI 1225 CTCGAG C^[CT]CG[GA]G 1942 CCCGAG BsrDI 1809 CATTGC GCAATG(2/0) 525 GCAATG BsrFI 1028 ACCGGC [GA]^CCGG[CT] 2092 GCCGGC Cfr10I 1028 ACCGGC [GA]^CCGG[CT] 2092 GCCGGC CpoI 487 CGGACCG CG^G[AT]CCG 1682 CGGACCG DraIII 679 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG 1282 CACGACGTG EagI 60 CGGCCG C^GGCCG 190 CGGCCG EclXI 60 CGGCCG C^GGCCG 190 CGGCCG Eco52I 60 CGGCCG C^GGCCG 190 CGGCCG KspI 158 CCGCGG CCGC^GG 187 CCGCGG PflFI 1454 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1840 GACCTGGTC RsrII 487 CGGACCG CG^G[AT]CCG 1682 CGGACCG SacII 158 CCGCGG CCGC^GG 187 CCGCGG SmlI 1225 CTCGAG C^T[CT][GA]AG 1531 CTCAAG TfiI 1601 GAATC G^A[AT]TC 1964 GATTC Tth111I 1454 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1840 GACCTGGTC XmnI 560 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1080 GAAGACATTC ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaI BsaJI BseRI BsiEI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII