(Circular) (Six-base) MAPSORT of: pFN21AE2064.seq Check: 2974 from: 1 to: 6841 pFN21AE2064 6841 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4651 278 Size: 53 83 186 936 3115 2468 Fragments arranged by size: 3115 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4184 1937 Size: 2247 4594 Acc65I G'GTAC_C Cuts at: 3903 4162 3903 Size: 259 6582 AclI AA'CG_TT Cuts at: 1563 4968 5341 1563 Size: 3405 373 3063 Fragments arranged by size: 3405 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3104 4113 4904 6288 6556 1632 Size: 1472 1009 791 1384 268 1917 Fragments arranged by size: 1917 1472 1384 1009 791 268 AfeI AGC'GCT Cuts at: 2138 2138 Size: 6841 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6644 AflIII A'CryG_T Cuts at: 1203 3504 5740 6717 1203 Size: 2301 2236 977 1327 Fragments arranged by size: 2301 2236 1327 977 AgeI A'CCGG_T Cuts at: 5672 5672 Size: 6841 AhdI GACnn_n'nnGTC Cuts at: 2296 5570 2296 Size: 3274 3567 AleI CACnn'nnGTG Cuts at: 1166 3206 1166 Size: 2040 4801 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2056 3439 1184 Size: 872 1383 4586 Fragments arranged by size: 4586 1383 872 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2762 2794 2762 Size: 32 6809 AlwNI CAG_nnn'CTG Cuts at: 2479 2617 2921 3322 3460 3625 3871 4109 4584 Size: 138 304 401 138 165 246 238 475 Cuts at: 4584 6156 2479 Size: 1572 3164 Fragments arranged by size: 3164 1572 475 401 304 246 238 165 138 138 ApaI G_GGCC'C Cuts at: 2287 2287 Size: 6841 ApaLI G'TGCA_C Cuts at: 3629 4898 6054 3629 Size: 1269 1156 4416 Fragments arranged by size: 4416 1269 1156 ApoI r'AATT_y Cuts at: 1493 4150 4376 4528 6637 1493 Size: 2657 226 152 2109 1697 Fragments arranged by size: 2657 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5395 160 Size: 872 4363 1606 Fragments arranged by size: 4363 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6841 AvaI C'yCGr_G Cuts at: 1870 1943 3230 3947 4166 1870 Size: 73 1287 717 219 4545 Fragments arranged by size: 4545 1287 717 219 73 AvrII C'CTAG_G Cuts at: 2536 2536 Size: 6841 BamHI G'GATC_C Cuts at: 2149 2721 4171 4538 2149 Size: 572 1450 367 4452 Fragments arranged by size: 4452 1450 572 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2115 2649 3903 4162 5617 Size: 325 217 627 328 534 1254 259 1455 Cuts at: 5617 618 Size: 1842 Fragments arranged by size: 1842 1455 1254 627 534 328 325 259 217 BanII G_rGCy'C Cuts at: 1391 2089 2192 2205 2268 2287 2892 4160 1391 Size: 698 103 13 63 19 605 1268 4072 Fragments arranged by size: 4072 1268 698 605 103 63 19 13 BbeI G_GCGC'C Cuts at: 2119 2119 Size: 6841 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2985 3092 928 Size: 971 1086 107 4677 Fragments arranged by size: 4677 1086 971 107 BbvCI CC'TCA_GC Cuts at: 2844 2914 3443 2844 Size: 70 529 6242 Fragments arranged by size: 6242 529 70 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5032 5066 1903 Size: 34 3095 34 3678 Fragments arranged by size: 3678 3095 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4733 5943 4733 Size: 1210 5631 BclI T'GATC_A Cuts at: 1548 1548 Size: 6841 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3158 3191 3233 Size: 107 122 71 631 605 1486 33 42 Cuts at: 3233 4055 5452 136 Size: 822 1397 1525 Fragments arranged by size: 1525 1486 1397 822 631 605 122 107 71 42 33 BglII A'GATC_T Cuts at: 6836 6836 Size: 6841 BlpI GC'TnA_GC Cuts at: 4258 4258 Size: 6841 Bme1580I G_kGCm'C Cuts at: 1790 2287 3633 4902 6058 1790 Size: 497 1346 1269 1156 2573 Fragments arranged by size: 2573 1346 1269 1156 497 BmgBI CAC'GTC Cuts at: 1318 3190 3292 1318 Size: 1872 102 4867 Fragments arranged by size: 4867 1872 102 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3190 4572 5530 Size: 416 53 83 399 24 1773 1382 958 Cuts at: 5530 442 Size: 1753 Fragments arranged by size: 1773 1753 1382 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6841 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2076 2719 2806 2076 Size: 643 87 6111 Fragments arranged by size: 6111 643 87 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2316 3395 3905 4010 5501 1239 Size: 1077 1079 510 105 1491 2579 Fragments arranged by size: 2579 1491 1079 1077 510 105 Bpu10I CC'TnA_GC Cuts at: 2186 2204 2844 2914 3443 2186 Size: 18 640 70 529 5584 Fragments arranged by size: 5584 640 529 70 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3521 4966 5831 6129 3521 Size: 1445 865 298 4233 Fragments arranged by size: 4233 1445 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3313 3545 3714 5504 882 Size: 617 1814 232 169 1790 2219 Fragments arranged by size: 2219 1814 1790 617 232 169 BsaAI yAC'GTr Cuts at: 493 1204 4632 493 Size: 711 3428 2702 Fragments arranged by size: 3428 2702 711 BsaBI GATnn'nnATC Cuts at: 6835 6835 Size: 6841 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2116 2462 3363 4648 Size: 53 83 186 936 583 346 901 1285 Cuts at: 4648 5030 5665 275 Size: 382 635 1451 Fragments arranged by size: 1451 1285 936 901 635 583 382 346 186 83 53 BsaWI w'CCGG_w Cuts at: 2152 5274 5672 5946 6093 6504 2152 Size: 3122 398 274 147 411 2489 Fragments arranged by size: 3122 2489 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6616 Fragments arranged by size: 6616 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2293 2410 2861 2902 2969 3572 4007 4028 Size: 1107 117 451 41 67 603 435 21 Cuts at: 4028 4040 1186 Size: 12 3987 Fragments arranged by size: 3987 1107 603 451 435 117 67 41 21 12 BseYI C'CCAG_C Cuts at: 3100 6044 6540 3100 Size: 2944 496 3401 Fragments arranged by size: 3401 2944 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6268 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2068 2198 3604 4291 5052 6080 Size: 275 186 67 130 1406 687 761 1028 Cuts at: 6080 1540 Size: 2301 Fragments arranged by size: 2301 1406 1028 761 687 275 186 130 67 BsiHKAI G_wGCw'C Cuts at: 2205 3633 4902 4987 6058 2205 Size: 1428 1269 85 1071 2988 Fragments arranged by size: 2988 1428 1269 1071 85 BsmI GAATG_Cn' Cuts at: 4352 4445 4352 Size: 93 6748 BsmBI CGTCTCn'nnnn_ Cuts at: 2621 2660 2621 Size: 39 6802 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2089 2192 2205 2268 2287 2892 3633 Size: 399 299 103 13 63 19 605 741 Cuts at: 3633 4160 4902 4987 6058 1391 Size: 527 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 741 605 527 399 299 103 85 63 19 13 BspEI T'CCGG_A Cuts at: 2152 6504 2152 Size: 4352 2489 BspHI T'CATG_A Cuts at: 4730 6460 4730 Size: 1730 5111 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2037 3087 3132 3879 4196 844 Size: 1193 1050 45 747 317 3489 Fragments arranged by size: 3489 1193 1050 747 317 45 BsrBI CCG'CTC Cuts at: 1394 1823 2373 2763 3073 3952 4728 1394 Size: 429 550 390 310 879 776 3507 Fragments arranged by size: 3507 879 776 550 429 390 310 BsrDI GCAATG_nn' Cuts at: 65 1248 2537 3813 5336 5510 65 Size: 1183 1289 1276 1523 174 1396 Fragments arranged by size: 1523 1396 1289 1276 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3033 4097 5485 5672 1700 Size: 110 1223 1064 1388 187 2869 Fragments arranged by size: 2869 1388 1223 1064 187 110 BsrGI T'GTAC_A Cuts at: 96 3354 96 Size: 3258 3583 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6841 BssSI C'ACGA_G Cuts at: 2269 4901 5913 2269 Size: 2632 1012 3197 Fragments arranged by size: 3197 2632 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3211 794 Size: 2417 4424 BstBI TT'CG_AA Cuts at: 5645 5645 Size: 6841 BstEII G'GTnAC_C Cuts at: 1184 2836 1184 Size: 1652 5189 BstYI r'GATC_y Cuts at: 1972 2149 2721 3096 3915 4171 4538 4923 4940 Size: 177 572 375 819 256 367 385 17 Cuts at: 4940 6392 6478 6490 6836 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 819 572 385 375 367 346 256 177 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2163 2192 3543 513 Size: 552 939 159 29 1351 3811 Fragments arranged by size: 3811 1351 939 552 159 29 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6102 Fragments arranged by size: 6102 683 56 BtsI GCAGTG_nn' Cuts at: 4357 5151 5171 4357 Size: 794 20 6027 Fragments arranged by size: 6027 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6841 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6806 DraI TTT'AAA Cuts at: 4145 4145 Size: 6841 DraIII CAC_nnn'GTG Cuts at: 2689 3292 2689 Size: 603 6238 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2746 5848 809 Size: 507 1430 3102 1802 Fragments arranged by size: 3102 1802 1430 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2065 2195 2427 Size: 54 1425 50 275 18 235 130 232 Cuts at: 2427 2481 2964 3114 3495 3528 3699 4288 5177 Size: 54 483 150 381 33 171 589 889 Cuts at: 5177 6806 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 589 483 381 275 235 232 171 150 130 54 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 2065 2195 4288 1537 Size: 275 253 130 2093 4090 Fragments arranged by size: 4090 2093 275 253 130 EarI CTCTTCn'nnn_ Cuts at: 1353 3999 4017 4029 4771 1353 Size: 2646 18 12 742 3423 Fragments arranged by size: 3423 2646 742 18 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3032 5428 5802 5948 387 Size: 1064 138 1443 2396 374 146 1280 Fragments arranged by size: 2396 1443 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2203 2203 Size: 6841 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2316 3104 3395 3905 4010 4113 4904 Size: 393 684 788 291 510 105 103 791 Cuts at: 4904 5501 6288 6556 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 791 788 787 684 597 510 393 291 268 105 103 EcoNI CCTnn'n_nnAGG Cuts at: 2239 2239 Size: 6841 EcoO109I rG'GnC_Cy Cuts at: 2182 2283 2284 2476 2555 2851 3076 3868 2182 Size: 101 1 192 79 296 225 792 5155 Fragments arranged by size: 5155 792 296 225 192 101 79 1 EcoRI G'AATT_C Cuts at: 4150 4528 4150 Size: 378 6463 EcoRV GAT'ATC Cuts at: 3430 3430 Size: 6841 FspI TGC'GCA Cuts at: 3470 5347 3470 Size: 1877 4964 HaeII r_GCGC'y Cuts at: 1678 2119 2140 2248 3282 3350 3450 5988 1678 Size: 441 21 108 1034 68 100 2538 2531 Fragments arranged by size: 2538 2531 1034 441 108 100 68 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2282 2314 3719 3751 5482 Size: 32 135 32 710 32 1405 32 1731 Cuts at: 5482 5514 5556 5588 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1731 1405 710 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2739 4185 4431 669 Size: 988 281 801 1446 246 3079 Fragments arranged by size: 3079 1446 988 801 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6841 HpaI GTT'AAC Cuts at: 4431 4431 Size: 6841 KasI G'GCGC_C Cuts at: 2115 2115 Size: 6841 KpnI G_GTAC'C Cuts at: 3907 4166 3907 Size: 259 6582 MfeI C'AATT_G Cuts at: 4440 4440 Size: 6841 MluI A'CGCG_T Cuts at: 6717 6717 Size: 6841 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2155 2542 2643 2730 3336 3624 5955 6139 Size: 850 387 101 87 606 288 2331 184 Cuts at: 6139 1305 Size: 2007 Fragments arranged by size: 2331 2007 850 606 387 288 184 101 87 MscI TGG'CCA Cuts at: 10 64 1489 1832 2429 2483 2966 3116 3497 Size: 54 1425 343 597 54 483 150 381 Cuts at: 3497 10 Size: 3354 Fragments arranged by size: 3354 1425 597 483 381 343 150 54 54 MslI CAynn'nnrTG Cuts at: 518 1166 2456 3153 3206 3485 3503 3858 4799 Size: 648 1290 697 53 279 18 355 941 Cuts at: 4799 5158 5317 518 Size: 359 159 2042 Fragments arranged by size: 2042 1290 941 697 648 359 355 279 159 53 18 MspA1I CmG'CkG Cuts at: 1606 2165 2194 2350 2843 3664 4257 4934 6082 Size: 559 29 156 493 821 593 677 1148 Cuts at: 6082 6327 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 821 677 593 559 493 245 156 29 NaeI GCC'GGC Cuts at: 4099 4099 Size: 6841 NarI GG'CG_CC Cuts at: 2116 2116 Size: 6841 NcoI C'CATG_G Cuts at: 513 1065 2004 3543 513 Size: 552 939 1539 3811 Fragments arranged by size: 3811 1539 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6841 NgoMIV G'CCGG_C Cuts at: 4097 4097 Size: 6841 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6841 NotI GC'GGCC_GC Cuts at: 2065 4288 2065 Size: 2223 4618 NspI r_CATG'y Cuts at: 1132 1229 3182 3209 3508 4199 5744 1132 Size: 97 1953 27 299 691 1545 2229 Fragments arranged by size: 2229 1953 1545 691 299 97 27 PasI CC'CwG_GG Cuts at: 1348 4109 1348 Size: 2761 4080 PciI A'CATG_T Cuts at: 3504 5740 3504 Size: 2236 4605 PflMI CCAn_nnn'nTGG Cuts at: 1108 2479 2933 2991 3292 3388 4109 1108 Size: 1371 454 58 301 96 721 3840 Fragments arranged by size: 3840 1371 721 454 301 96 58 PfoI T'CCnGG_A Cuts at: 2765 2792 2802 2867 3585 2765 Size: 27 10 65 718 6021 Fragments arranged by size: 6021 718 65 27 10 PmeI GTTT'AAAC Cuts at: 4145 4145 Size: 6841 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3224 3256 4910 4942 6423 6455 3224 Size: 32 1654 32 1481 32 3610 Fragments arranged by size: 3610 1654 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2182 2476 2555 3076 3868 2182 Size: 294 79 521 792 5155 Fragments arranged by size: 5155 792 521 294 79 PshAI GACnn'nnGTC Cuts at: 1613 3750 1613 Size: 2137 4704 PsiI TTA'TAA Cuts at: 4411 4411 Size: 6841 PspOMI G'GGCC_C Cuts at: 2283 2283 Size: 6841 PspXI vC'TCGA_Gb Cuts at: 3230 3230 Size: 6841 PstI C_TGCA'G Cuts at: 830 2243 2599 2676 3414 4193 830 Size: 1413 356 77 738 779 3478 Fragments arranged by size: 3478 1413 779 738 356 77 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6841 RsrII CG'GwC_CG Cuts at: 2493 3688 2493 Size: 1195 5646 SacI G_AGCT'C Cuts at: 2205 2205 Size: 6841 SacII CC_GC'GG Cuts at: 2166 2195 2166 Size: 29 6812 SalI G'TCGA_C Cuts at: 1936 4183 1936 Size: 2247 4594 SanDI GG'GwC_CC Cuts at: 2555 2555 Size: 6841 SbfI CC_TGCA'GG Cuts at: 2243 4193 2243 Size: 1950 4891 ScaI AGT'ACT Cuts at: 5089 6801 5089 Size: 1712 5129 SexAI A'CCwGG_T Cuts at: 2772 3624 3846 2772 Size: 852 222 5767 Fragments arranged by size: 5767 852 222 SfcI C'TryA_G Cuts at: 826 1045 2239 2595 2672 3410 4189 5324 6005 Size: 219 1194 356 77 738 779 1135 681 Cuts at: 6005 6196 826 Size: 191 1471 Fragments arranged by size: 1471 1194 1135 779 738 681 356 219 191 77 SfoI GGC'GCC Cuts at: 2117 2117 Size: 6841 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6841 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6841 SmaI CCC'GGG Cuts at: 1872 4168 1872 Size: 2296 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3230 3536 4945 5846 6108 820 Size: 197 926 1287 306 1409 901 262 1553 Fragments arranged by size: 1553 1409 1287 926 901 306 262 197 SnaBI TAC'GTA Cuts at: 493 4632 493 Size: 4139 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6841 SphI G_CATG'C Cuts at: 1132 4199 1132 Size: 3067 3774 SspI AAT'ATT Cuts at: 5 52 4765 5 Size: 47 4713 2081 Fragments arranged by size: 4713 2081 47 StuI AGG'CCT Cuts at: 1517 4058 1517 Size: 2541 4300 StyI C'CwwG_G Cuts at: 513 1065 2004 2400 2536 2832 2967 3543 4059 Size: 552 939 396 136 296 135 576 516 Cuts at: 4059 4280 513 Size: 221 3074 Fragments arranged by size: 3074 939 576 552 516 396 296 221 136 135 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2831 3618 3837 3870 4868 4885 5038 Size: 1255 1251 787 219 33 998 17 153 Cuts at: 5038 5223 5668 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1251 998 787 445 219 185 153 33 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2784 3354 5087 Size: 274 80 33 51 1443 807 570 1733 Cuts at: 5087 6799 96 Size: 1712 138 Fragments arranged by size: 1733 1712 1443 807 570 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2988 3020 6801 6833 2988 Size: 32 3781 32 2996 Fragments arranged by size: 3781 2996 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3462 3848 1316 Size: 2146 386 4309 Fragments arranged by size: 4309 2146 386 XbaI T'CTAG_A Cuts at: 4177 4177 Size: 6841 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2308 3289 3340 4057 2011 Size: 297 981 51 717 4795 Fragments arranged by size: 4795 981 717 297 51 XhoI C'TCGA_G Cuts at: 1943 3230 1943 Size: 1287 5554 XmaI C'CCGG_G Cuts at: 1870 4166 1870 Size: 2296 4545 XmnI GAAnn'nnTTC Cuts at: 2569 3089 4970 2569 Size: 520 1881 4440 Fragments arranged by size: 4440 1881 520 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4649 276 Size: 53 83 186 936 3115 2468 Fragments arranged by size: 3115 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I FalI FseI FspAI NruI NsiI PacI PmlI PsrI PvuII SapI SfiI SrfI SwaI