(Linear) (Six-base) MAPSORT of: KIEE2064.seq Check: 2494 from: 1 to: 2136 KIEE2064.orf With 182 enzymes: SgfI * July 15, 2010 18:54 .. Acc65I G'GTAC_C Cuts at: 0 1898 2136 Size: 1898 238 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1099 2108 2136 Size: 1099 1009 28 Fragments arranged by size: 1099 1009 28 AfeI AGC'GCT Cuts at: 0 133 2136 Size: 133 2003 AflIII A'CryG_T Cuts at: 0 1499 2136 Size: 1499 637 AhdI GACnn_n'nnGTC Cuts at: 0 291 2136 Size: 291 1845 AleI CACnn'nnGTG Cuts at: 0 1201 2136 Size: 1201 935 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 51 1434 2136 Size: 51 1383 702 Fragments arranged by size: 1383 702 51 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 757 789 2136 Size: 757 32 1347 Fragments arranged by size: 1347 757 32 AlwNI CAG_nnn'CTG Cuts at: 0 474 612 916 1317 1455 1620 1866 2104 Size: 474 138 304 401 138 165 246 238 Cuts at: 2104 2136 Size: 32 Fragments arranged by size: 474 401 304 246 238 165 138 138 32 ApaI G_GGCC'C Cuts at: 0 282 2136 Size: 282 1854 ApaLI G'TGCA_C Cuts at: 0 1624 2136 Size: 1624 512 AvaI C'yCGr_G Cuts at: 0 1225 1942 2136 Size: 1225 717 194 Fragments arranged by size: 1225 717 194 AvrII C'CTAG_G Cuts at: 0 531 2136 Size: 531 1605 BamHI G'GATC_C Cuts at: 0 144 716 2136 Size: 144 572 1420 Fragments arranged by size: 1420 572 144 BanI G'GyrC_C Cuts at: 0 110 644 1898 2136 Size: 110 534 1254 238 Fragments arranged by size: 1254 534 238 110 BanII G_rGCy'C Cuts at: 0 84 187 200 263 282 887 2136 Size: 84 103 13 63 19 605 1249 Fragments arranged by size: 1249 605 103 84 63 19 13 BbeI G_GCGC'C Cuts at: 0 114 2136 Size: 114 2022 BbsI GAAGACnn'nnnn_ Cuts at: 0 980 1087 2136 Size: 980 107 1049 Fragments arranged by size: 1049 980 107 BbvCI CC'TCA_GC Cuts at: 0 839 909 1438 2136 Size: 839 70 529 698 Fragments arranged by size: 839 698 529 70 BglI GCCn_nnn'nGGC Cuts at: 0 1153 1186 1228 2050 2136 Size: 1153 33 42 822 86 Fragments arranged by size: 1153 822 86 42 33 Bme1580I G_kGCm'C Cuts at: 0 282 1628 2136 Size: 282 1346 508 Fragments arranged by size: 1346 508 282 BmgBI CAC'GTC Cuts at: 0 1185 1287 2136 Size: 1185 102 849 Fragments arranged by size: 1185 849 102 BmrI ACTGGGnnnn_n' Cuts at: 0 1185 2136 Size: 1185 951 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 71 714 801 2136 Size: 71 643 87 1335 Fragments arranged by size: 1335 643 87 71 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 311 1390 1900 2005 2136 Size: 311 1079 510 105 131 Fragments arranged by size: 1079 510 311 131 105 Bpu10I CC'TnA_GC Cuts at: 0 181 199 839 909 1438 2136 Size: 181 18 640 70 529 698 Fragments arranged by size: 698 640 529 181 70 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1516 2136 Size: 1516 620 BsaI GGTCTCn'nnnn_ Cuts at: 0 1308 1540 1709 2136 Size: 1308 232 169 427 Fragments arranged by size: 1308 427 232 169 BsaHI Gr'CG_yC Cuts at: 0 111 457 1358 2136 Size: 111 346 901 778 Fragments arranged by size: 901 778 346 111 BsaWI w'CCGG_w Cuts at: 0 147 2136 Size: 147 1989 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 288 405 856 897 964 1567 2002 2023 Size: 288 117 451 41 67 603 435 21 Cuts at: 2023 2035 2136 Size: 12 101 Fragments arranged by size: 603 451 435 288 117 101 67 41 21 12 BseYI C'CCAG_C Cuts at: 0 1095 2136 Size: 1095 1041 BsiEI CG_ry'CG Cuts at: 0 63 193 1599 2136 Size: 63 130 1406 537 Fragments arranged by size: 1406 537 130 63 BsiHKAI G_wGCw'C Cuts at: 0 200 1628 2136 Size: 200 1428 508 Fragments arranged by size: 1428 508 200 BsmBI CGTCTCn'nnnn_ Cuts at: 0 616 655 2136 Size: 616 39 1481 Fragments arranged by size: 1481 616 39 Bsp1286I G_dGCh'C Cuts at: 0 84 187 200 263 282 887 1628 2136 Size: 84 103 13 63 19 605 741 508 Fragments arranged by size: 741 605 508 103 84 63 19 13 BspEI T'CCGG_A Cuts at: 0 147 2136 Size: 147 1989 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 32 1082 1127 1874 2136 Size: 32 1050 45 747 262 Fragments arranged by size: 1050 747 262 45 32 BsrBI CCG'CTC Cuts at: 0 368 758 1068 1947 2136 Size: 368 390 310 879 189 Fragments arranged by size: 879 390 368 310 189 BsrDI GCAATG_nn' Cuts at: 0 532 1808 2136 Size: 532 1276 328 Fragments arranged by size: 1276 532 328 BsrFI r'CCGG_y Cuts at: 0 1028 2092 2136 Size: 1028 1064 44 Fragments arranged by size: 1064 1028 44 BsrGI T'GTAC_A Cuts at: 0 1349 2136 Size: 1349 787 BssSI C'ACGA_G Cuts at: 0 264 2136 Size: 264 1872 BstAPI GCAn_nnn'nTGC Cuts at: 0 1206 2136 Size: 1206 930 BstEII G'GTnAC_C Cuts at: 0 831 2136 Size: 831 1305 BstYI r'GATC_y Cuts at: 0 144 716 1091 1910 2136 Size: 144 572 375 819 226 Fragments arranged by size: 819 572 375 226 144 BtgI C'CryG_G Cuts at: 0 158 187 1538 2136 Size: 158 29 1351 598 Fragments arranged by size: 1351 598 158 29 DraIII CAC_nnn'GTG Cuts at: 0 684 1287 2136 Size: 684 603 849 Fragments arranged by size: 849 684 603 DrdI GACnn_nn'nnGTC Cuts at: 0 741 2136 Size: 741 1395 EaeI y'GGCC_r Cuts at: 0 60 190 422 476 959 1109 1490 1523 Size: 60 130 232 54 483 150 381 33 Cuts at: 1523 1694 2136 Size: 171 442 Fragments arranged by size: 483 442 381 232 171 150 130 60 54 33 EagI C'GGCC_G Cuts at: 0 60 190 2136 Size: 60 130 1946 Fragments arranged by size: 1946 130 60 EarI CTCTTCn'nnn_ Cuts at: 0 1994 2012 2024 2136 Size: 1994 18 12 112 Fragments arranged by size: 1994 112 18 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1027 2136 Size: 1027 1109 EcoICRI GAG'CTC Cuts at: 0 198 2136 Size: 198 1938 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 311 1099 1390 1900 2005 2108 2136 Size: 311 788 291 510 105 103 28 Fragments arranged by size: 788 510 311 291 105 103 28 EcoNI CCTnn'n_nnAGG Cuts at: 0 234 2136 Size: 234 1902 EcoO109I rG'GnC_Cy Cuts at: 0 177 278 279 471 550 846 1071 1863 Size: 177 101 1 192 79 296 225 792 Cuts at: 1863 2136 Size: 273 Fragments arranged by size: 792 296 273 225 192 177 101 79 1 EcoRV GAT'ATC Cuts at: 0 1425 2136 Size: 1425 711 FspI TGC'GCA Cuts at: 0 1465 2136 Size: 1465 671 HaeII r_GCGC'y Cuts at: 0 114 135 243 1277 1345 1445 2136 Size: 114 21 108 1034 68 100 691 Fragments arranged by size: 1034 691 114 108 100 68 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 277 309 1714 1746 2136 Size: 277 32 1405 32 390 Fragments arranged by size: 1405 390 277 32 32 HincII GTy'rAC Cuts at: 0 734 2136 Size: 734 1402 KasI G'GCGC_C Cuts at: 0 110 2136 Size: 110 2026 KpnI G_GTAC'C Cuts at: 0 1902 2136 Size: 1902 234 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 150 537 638 725 1331 1619 2136 Size: 150 387 101 87 606 288 517 Fragments arranged by size: 606 517 387 288 150 101 87 MscI TGG'CCA Cuts at: 0 424 478 961 1111 1492 2136 Size: 424 54 483 150 381 644 Fragments arranged by size: 644 483 424 381 150 54 MslI CAynn'nnrTG Cuts at: 0 451 1148 1201 1480 1498 1853 2136 Size: 451 697 53 279 18 355 283 Fragments arranged by size: 697 451 355 283 279 53 18 MspA1I CmG'CkG Cuts at: 0 160 189 345 838 1659 2136 Size: 160 29 156 493 821 477 Fragments arranged by size: 821 493 477 160 156 29 NaeI GCC'GGC Cuts at: 0 2094 2136 Size: 2094 42 NarI GG'CG_CC Cuts at: 0 111 2136 Size: 111 2025 NcoI C'CATG_G Cuts at: 0 1538 2136 Size: 1538 598 NgoMIV G'CCGG_C Cuts at: 0 2092 2136 Size: 2092 44 NotI GC'GGCC_GC Cuts at: 0 60 2136 Size: 60 2076 NspI r_CATG'y Cuts at: 0 1177 1204 1503 2136 Size: 1177 27 299 633 Fragments arranged by size: 1177 633 299 27 PasI CC'CwG_GG Cuts at: 0 2104 2136 Size: 2104 32 PciI A'CATG_T Cuts at: 0 1499 2136 Size: 1499 637 PflMI CCAn_nnn'nTGG Cuts at: 0 474 928 986 1287 1383 2104 2136 Size: 474 454 58 301 96 721 32 Fragments arranged by size: 721 474 454 301 96 58 32 PfoI T'CCnGG_A Cuts at: 0 760 787 797 862 1580 2136 Size: 760 27 10 65 718 556 Fragments arranged by size: 760 718 556 65 27 10 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1219 1251 2136 Size: 1219 32 885 Fragments arranged by size: 1219 885 32 PpuMI rG'GwC_Cy Cuts at: 0 177 471 550 1071 1863 2136 Size: 177 294 79 521 792 273 Fragments arranged by size: 792 521 294 273 177 79 PshAI GACnn'nnGTC Cuts at: 0 1745 2136 Size: 1745 391 PspOMI G'GGCC_C Cuts at: 0 278 2136 Size: 278 1858 PspXI vC'TCGA_Gb Cuts at: 0 1225 2136 Size: 1225 911 PstI C_TGCA'G Cuts at: 0 238 594 671 1409 2136 Size: 238 356 77 738 727 Fragments arranged by size: 738 727 356 238 77 RsrII CG'GwC_CG Cuts at: 0 488 1683 2136 Size: 488 1195 453 Fragments arranged by size: 1195 488 453 SacI G_AGCT'C Cuts at: 0 200 2136 Size: 200 1936 SacII CC_GC'GG Cuts at: 0 161 190 2136 Size: 161 29 1946 Fragments arranged by size: 1946 161 29 SanDI GG'GwC_CC Cuts at: 0 550 2136 Size: 550 1586 SbfI CC_TGCA'GG Cuts at: 0 238 2136 Size: 238 1898 SexAI A'CCwGG_T Cuts at: 0 767 1619 1841 2136 Size: 767 852 222 295 Fragments arranged by size: 852 767 295 222 SfcI C'TryA_G Cuts at: 0 234 590 667 1405 2136 Size: 234 356 77 738 731 Fragments arranged by size: 738 731 356 234 77 SfoI GGC'GCC Cuts at: 0 112 2136 Size: 112 2024 SmlI C'TyrA_G Cuts at: 0 1225 1531 2136 Size: 1225 306 605 Fragments arranged by size: 1225 605 306 StuI AGG'CCT Cuts at: 0 2053 2136 Size: 2053 83 StyI C'CwwG_G Cuts at: 0 395 531 827 962 1538 2054 2136 Size: 395 136 296 135 576 516 82 Fragments arranged by size: 576 516 395 296 136 135 82 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 826 1613 1832 1865 2136 Size: 826 787 219 33 271 Fragments arranged by size: 826 787 271 219 33 TatI w'GTAC_w Cuts at: 0 779 1349 2136 Size: 779 570 787 Fragments arranged by size: 787 779 570 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 983 1015 2136 Size: 983 32 1121 Fragments arranged by size: 1121 983 32 Tth111I GACn'n_nGTC Cuts at: 0 1457 1843 2136 Size: 1457 386 293 Fragments arranged by size: 1457 386 293 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 303 1284 1335 2052 2136 Size: 303 981 51 717 84 Fragments arranged by size: 981 717 303 84 51 XhoI C'TCGA_G Cuts at: 0 1225 2136 Size: 1225 911 XmnI GAAnn'nnTTC Cuts at: 0 564 1084 2136 Size: 564 520 1052 Fragments arranged by size: 1052 564 520 Enzymes that do cut: Acc65I AcuI AfeI AflIII AhdI AleI AlfI AloI AlwNI ApaI ApaLI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstYI BtgI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PciI PflMI PfoI PpiI PpuMI PshAI PspOMI PspXI PstI RsrII SacI SacII SanDI SbfI SexAI SfcI SfoI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmnI Enzymes that do not cut: AarI AatII AccI AclI AflII AgeI ApoI AscI AseI AsiSI BaeI BcgI BciVI BclI BfrBI BglII BlpI BmtI BsaAI BsaBI BsaXI BsgI BsiWI BsmI BspHI BssHII BstBI BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI EcoRI FalI FseI FspAI HindIII HpaI MfeI MluI NdeI NheI NruI NsiI PacI PmeI PmlI PsiI PsrI PvuI PvuII SalI SapI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI XmaI ZraI