Restriction map for
pFN21AE5364
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbrPI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsoBI Bsp1407I BspEI BspMI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I Cfr10I
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MaeI MaeII
MamI MfeI MluI MroI MseI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfcI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AcyI 143 GGCGCC G[GA]^CG[CT]C
AfaI 282 GTAC GT^AC
AflIII 545 ACATGT A^C[GA][CT]GT
AlwI 537 GATCC GGATC(4/5)
AspHI 815 GAGCTC G[AT]GC[AT]^C
BalI 356 TGGCCA TGG^CCA
BbeI 143 GGCGCC GGCGC^C
BbsI 70 GTCTTC GAAGAC(2/6)
BbvCI 269 CCTCAGC CCTCAGC(-5/-2)
BcnI 140 CCCGG CC^[GC]GG
BglI 883 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 70 GTCTTC GAAGAC(2/6)
BsaHI 143 GGCGCC G[GA]^CG[CT]C
BsaI 526 GAGACC GGTCTC(1/5)
BseRI 850 GAGGAG GAGGAG(10/8)
BsgI 541 CTGCAC GTGCAG(16/14)
BsiHKAI 815 GAGCTC G[AT]GC[AT]^C
BsiYI 555 CCCCACCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 555 CCCCACCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 93 CGTCTC CGTCTC(1/5)
BsmI 822 GCATTC GAATGC(1/-1)
BspDI 232 ATCGAT AT^CGAT
BspHI 440 TCATGA T^CATGA
BspLU11I 545 ACATGT A^CATGT
BsrBI 58 CCGCTC CCGCTC(-3/-3)
BsrDI 181 CATTGC GCAATG(2/0)
BstEII 763 GGTGACC G^GT[ACGT]ACC
BstPI 763 GGTGACC G^GT[ACGT]ACC
BstXI 795 CCACAGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 536 AGATCC [GA]^GATC[CT]
ClaI 232 ATCGAT AT^CGAT
DraII 790 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 790 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 763 GGTGACC G^GT[ACGT]ACC
HapII 141 CCGG C^CGG
HgaI 157 GCGTC GACGC(5/10)
Hin1I 143 GGCGCC G[GA]^CG[CT]C
HinfI 377 GAGTC G^A[ACGT]TC
HpaII 141 CCGG C^CGG
KasI 143 GGCGCC G^GCGCC
MflI 536 AGATCC [GA]^GATC[CT]
MluNI 356 TGGCCA TGG^CCA
MscI 356 TGGCCA TGG^CCA
MspI 141 CCGG C^CGG
NarI 143 GGCGCC GG^CGCC
NciI 140 CCCGG CC^[GC]GG
NcoI 783 CCATGG C^CATGG
NspI 545 ACATGT [GA]CATG^[CT]
PleI 377 GAGTC GAGTC(4/5)
PpuMI 790 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 440 TCATGA T^CATGA
RsaI 282 GTAC GT^AC
SacI 815 GAGCTC GAGCT^C
SapI 60 GCTCTTC GCTCTTC(1/4)
SfoI 143 GGCGCC GGC^GCC
SmlI 109 CTCAAG C^T[CT][GA]AG
Tsp45I 764 GTGAC ^GT[GC]AC
XhoII 536 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AvaII 306 GGACC G^G[AT]CC
791 GGTCC
BanI 54 GGTGCC G^G[CT][GA]CC
143 GGCGCC
BanII 815 GAGCTC G[GA]GC[CT]^C
864 GGGCTC
BmyI 815 GAGCTC G[AGT]GC[ACT]^C
864 GGGCTC
BsmAI 526 GAGAC GTCTC(1/5)
94 GTCTC
BsmFI 138 GTCCC GGGAC(10/14)
792 GTCCC
Bsp1286I 815 GAGCTC G[AGT]GC[ACT]^C
864 GGGCTC
DsaI 426 CCACGG C^C[GA][CT]GG
783 CCATGG
EaeI 356 TGGCCA [CT]^GGCC[GA]
827 CGGCCA
MaeIII 83 GTTAC ^GT[ACGT]AC
764 GTGAC
MslI 348 CATCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
796 CACAGCGGTG
PvuII 272 CAGCTG CAG^CTG
499 CAGCTG
TspRI 227 CACTG CA[GC]TG[ACGT][ACGT]^
617 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AccII AciI AluI BbvI BlpI
BpmI Bpu1102I BsaJI BsrI BssKI
BstF5I BstNI BstUI Cac8I CelII
CfoI Cfr13I DdeI DpnII EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HhaI HinP1I HphI
ItaI Ksp632I MboI MboII MnlI
MspA1I MvaI MvnI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TseI TthHB8I
XcmI