Restriction map for
pFN21AE5435
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
ApaI ApoI AscI AseI AsnI
Asp718I AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BcgI
BciVI BclI BcnI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaHI
BsaI BsaWI BseAI BsgI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
EclXI Eco52I EcoNI EcoO109I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HapII HgaI Hin1I HincII
HindII HindIII HinfI HpaI HpaII
KasI KpnI MaeI MfeI MluI
MroI MseI MspI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI StyI SwaI
TaqI TfiI Tru9I Tth111I TthHB8I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AhdI 250 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 751 GTGCAC G^TGCAC
AlwNI 799 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 876 CCTCAGG CC^T[ACGT]AGG
Aor51HI 266 AGCGCT AGC^GCT
ApaLI 751 GTGCAC G^TGCAC
Asp700I 909 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 250 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 840 GGTCC G^G[AT]CC
BanII 408 GAGCCC G[GA]GC[CT]^C
BbvCI 855 CCTCAGC CCTCAGC(-5/-2)
BsaAI 367 TACGTG [CT]AC^GT[GA]
BsaBI 822 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 360 CGACCG CG[GA][CT]^CG
BsmFI 794 GTCCC GGGAC(10/14)
BspHI 404 TCATGA T^CATGA
BstEII 474 GGTGACC G^GT[ACGT]ACC
BstPI 474 GGTGACC G^GT[ACGT]ACC
BstXI 590 CCAAAGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 876 CCTCAGG CC^T[ACGT]AGG
Eam1105I 250 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 266 AGCGCT AGC^GCT
Eco81I 876 CCTCAGG CC^T[ACGT]AGG
EcoO65I 474 GGTGACC G^GT[ACGT]ACC
HaeII 266 AGCGCT [GA]GCGC^[CT]
KspI 412 CCGCGG CCGC^GG
MamI 822 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspI 239 GCATGC [GA]CATG^[CT]
PvuII 70 CAGCTG CAG^CTG
RcaI 404 TCATGA T^CATGA
SacII 412 CCGCGG CCGC^GG
SapI 78 GCTCTTC GCTCTTC(1/4)
SphI 239 GCATGC GCATG^C
StuI 710 AGGCCT AGG^CCT
Tsp509I 32 AATT ^AATT
XcmI 272 CCATCGCCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 909 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccII 236 CGCG CG^CG
413 CGCG
AfaI 102 GTAC GT^AC
177 GTAC
AspHI 400 GTGCTC G[AT]GC[AT]^C
751 GTGCAC
BbsI 909 GAAGAC GAAGAC(2/6)
55 GTCTTC
BpmI 67 CTCCAG CTGGAG(16/14)
147 CTGGAG
BpuAI 909 GAAGAC GAAGAC(2/6)
55 GTCTTC
BseRI 637 CTCCTC GAGGAG(10/8)
646 CTCCTC
BsiHKAI 400 GTGCTC G[AT]GC[AT]^C
751 GTGCAC
BsmBI 201 CGTCTC CGTCTC(1/5)
569 GAGACG
BspMI 125 ACCTGC ACCTGC(4/8)
251 ACCTGC
BsrI 293 CCAGT ACTGG(1/-1)
798 CCAGT
BstUI 236 CGCG CG^CG
413 CGCG
Cfr13I 618 GGCCC G^G[ACGT]CC
840 GGTCC
DdeI 856 CTCAG C^T[ACGT]AG
877 CTCAG
DsaI 221 CCGTGG C^C[GA][CT]GG
412 CCGCGG
EarI 79 CTCTTC CTCTTC(1/4)
181 CTCTTC
Ksp632I 79 CTCTTC CTCTTC(1/4)
181 CTCTTC
MaeII 368 ACGT A^CGT
398 ACGT
MspA1I 70 CAGCTG C[AC]G^C[GT]G
412 CCGCGG
MvnI 236 CGCG CG^CG
413 CGCG
PflMI 140 CCACCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
798 CCAGTCTCTGG
RsaI 102 GTAC GT^AC
177 GTAC
Sau96I 618 GGCCC G^G[ACGT]CC
840 GGTCC
SfaNI 75 GATGC GCATC(5/9)
827 GCATC
Tsp45I 25 GTGAC ^GT[GC]AC
475 GTGAC
TspRI 23 CAGTG CA[GC]TG[ACGT][ACGT]^
738 CACTG
Van91I 140 CCACCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
798 CCAGTCTCTGG
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AlwI BalI BbvI
BglI BmyI BsaJI BsiYI BslI
BsmAI Bsp1286I BssKI BstF5I BstNI
BstYI Cac8I CfoI DpnII EaeI
EcoRII FauI Fnu4HI FokI HaeIII
HhaI HinP1I HphI ItaI MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MvaI MwoI NdeII
NlaIII Sau3AI ScrFI TseI XhoII