(Circular) (Six-base) MAPSORT of: pFN21AE5435.seq Check: 64 from: 1 to: 5659 pFN21AE5435 5659 bp With 182 enzymes: SgfI * December 8, 2014 13:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3469 278 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3002 1937 Size: 1065 4594 Acc65I G'GTAC_C Cuts at: 2980 2980 Size: 5659 AclI AA'CG_TT Cuts at: 1563 3786 4159 1563 Size: 2223 373 3063 Fragments arranged by size: 3063 2223 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2302 2721 2807 2860 2908 2918 3722 5106 Size: 670 419 86 53 48 10 804 1384 Cuts at: 5106 5374 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 804 670 419 268 86 53 48 10 AfeI AGC'GCT Cuts at: 2273 2273 Size: 5659 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5462 AflIII A'CryG_T Cuts at: 1203 4558 5535 1203 Size: 3355 977 1327 Fragments arranged by size: 3355 1327 977 AgeI A'CCGG_T Cuts at: 4490 4490 Size: 5659 AhdI GACnn_n'nnGTC Cuts at: 2260 4388 2260 Size: 2128 3531 AleI CACnn'nnGTG Cuts at: 1166 2747 1166 Size: 1581 4078 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5659 AlwNI CAG_nnn'CTG Cuts at: 2809 3402 4974 2809 Size: 593 1572 3494 Fragments arranged by size: 3494 1572 593 ApaLI G'TGCA_C Cuts at: 2756 3716 4872 2756 Size: 960 1156 3543 Fragments arranged by size: 3543 1156 960 ApoI r'AATT_y Cuts at: 1493 2968 3194 3346 5455 1493 Size: 1475 226 152 2109 1697 Fragments arranged by size: 2109 1697 1475 226 152 AseI AT'TA_AT Cuts at: 160 1032 4213 160 Size: 872 3181 1606 Fragments arranged by size: 3181 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 AvaI C'yCGr_G Cuts at: 1870 1943 2984 1870 Size: 73 1041 4545 Fragments arranged by size: 4545 1041 73 BamHI G'GATC_C Cuts at: 2989 3356 2989 Size: 367 5292 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2980 4435 618 Size: 325 217 627 1193 1455 1842 Fragments arranged by size: 1842 1455 1193 627 325 217 BanII G_rGCy'C Cuts at: 1391 2417 2978 1391 Size: 1026 561 4072 Fragments arranged by size: 4072 1026 561 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2053 2921 928 Size: 971 154 868 3666 Fragments arranged by size: 3666 971 868 154 BbvCI CC'TCA_GC Cuts at: 2861 2861 Size: 5659 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3850 3884 1903 Size: 34 50 34 1829 34 3678 Fragments arranged by size: 3678 1829 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3551 4761 3551 Size: 1210 4449 BclI T'GATC_A Cuts at: 1548 1548 Size: 5659 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2288 2421 2439 Size: 107 122 71 631 605 616 133 18 Cuts at: 2439 4270 136 Size: 1831 1525 Fragments arranged by size: 1831 1525 631 616 605 133 122 107 71 18 BglII A'GATC_T Cuts at: 5654 5654 Size: 5659 BlpI GC'TnA_GC Cuts at: 3076 3076 Size: 5659 Bme1580I G_kGCm'C Cuts at: 1790 2760 3720 4876 1790 Size: 970 960 1156 2573 Fragments arranged by size: 2573 1156 970 960 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5659 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2797 3390 4348 Size: 416 53 83 399 24 1380 593 958 Cuts at: 4348 442 Size: 1753 Fragments arranged by size: 1753 1380 958 593 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5659 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2057 2173 4319 1239 Size: 818 116 2146 2579 Fragments arranged by size: 2579 2146 818 116 Bpu10I CC'TnA_GC Cuts at: 2861 2861 Size: 5659 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3784 4649 4947 3784 Size: 865 298 4496 Fragments arranged by size: 4496 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4322 882 Size: 617 2823 2219 Fragments arranged by size: 2823 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2374 3450 493 Size: 711 1170 1076 2702 Fragments arranged by size: 2702 1170 1076 711 BsaBI GATnn'nnATC Cuts at: 2831 5653 2831 Size: 2822 2837 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3466 3848 4483 275 Size: 53 83 186 936 1933 382 635 1451 Fragments arranged by size: 1933 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4092 4490 4764 4911 5322 4092 Size: 398 274 147 411 4429 Fragments arranged by size: 4429 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2565 2595 1178 Size: 30 165 30 1162 30 4242 Fragments arranged by size: 4242 1162 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2633 2642 1186 Size: 1447 9 4203 Fragments arranged by size: 4203 1447 9 BseYI C'CCAG_C Cuts at: 2122 2268 2629 4862 5358 2122 Size: 146 361 2233 496 2423 Fragments arranged by size: 2423 2233 496 361 146 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5086 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2368 3109 3870 4898 1540 Size: 275 186 367 741 761 1028 2301 Fragments arranged by size: 2301 1028 761 741 367 275 186 BsiHKAI G_wGCw'C Cuts at: 2409 2760 3720 3805 4876 2409 Size: 351 960 85 1071 3192 Fragments arranged by size: 3192 1071 960 351 85 BsmI GAATG_Cn' Cuts at: 3170 3263 3170 Size: 93 5566 BsmBI CGTCTCn'nnnn_ Cuts at: 2212 2568 2212 Size: 356 5303 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2409 2417 2760 2978 3720 3805 4876 Size: 399 619 8 343 218 742 85 1071 Cuts at: 4876 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 619 399 343 218 85 8 BspEI T'CCGG_A Cuts at: 5322 5322 Size: 5659 BspHI T'CATG_A Cuts at: 2409 3548 5278 2409 Size: 1139 1730 2790 Fragments arranged by size: 2790 1730 1139 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2139 2265 3014 844 Size: 1295 126 749 3489 Fragments arranged by size: 3489 1295 749 126 BsrBI CCG'CTC Cuts at: 1394 1823 3546 1394 Size: 429 1723 3507 Fragments arranged by size: 3507 1723 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4154 4328 65 Size: 1183 2906 174 1396 Fragments arranged by size: 2906 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4303 4490 1700 Size: 110 2493 187 2869 Fragments arranged by size: 2869 2493 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5659 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5659 BssSI C'ACGA_G Cuts at: 3719 4731 3719 Size: 1012 4647 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5659 BstBI TT'CG_AA Cuts at: 4463 4463 Size: 5659 BstEII G'GTnAC_C Cuts at: 1184 2479 1184 Size: 1295 4364 BstXI CCAn_nnnn'nTGG Cuts at: 2602 2602 Size: 5659 BstYI r'GATC_y Cuts at: 1972 2214 2523 2688 2989 3356 3741 3758 5210 Size: 242 309 165 301 367 385 17 1452 Cuts at: 5210 5296 5308 5654 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 309 301 242 165 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2882 2882 Size: 5659 BtgI C'CryG_G Cuts at: 513 1065 2226 2417 513 Size: 552 1161 191 3755 Fragments arranged by size: 3755 1161 552 191 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2263 487 Size: 683 56 1037 3883 Fragments arranged by size: 3883 1037 683 56 BtsI GCAGTG_nn' Cuts at: 3175 3969 3989 3175 Size: 794 20 4845 Fragments arranged by size: 4845 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5659 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2538 2573 529 Size: 35 1974 35 3615 Fragments arranged by size: 3615 1974 35 35 DraI TTT'AAA Cuts at: 2963 2963 Size: 5659 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4666 809 Size: 507 3350 1802 Fragments arranged by size: 3350 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2142 2229 2250 Size: 54 1425 50 275 18 312 87 21 Cuts at: 2250 2376 2487 2592 2673 3106 3995 5624 8 Size: 126 111 105 81 433 889 1629 43 Fragments arranged by size: 1629 1425 889 433 312 275 126 111 105 87 81 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 3106 1537 Size: 275 1294 4090 Fragments arranged by size: 4090 1294 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2090 2192 3589 1353 Size: 737 102 1397 3423 Fragments arranged by size: 3423 1397 737 102 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4246 4620 4766 387 Size: 1064 138 2657 374 146 1280 Fragments arranged by size: 2657 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2173 2302 2721 2807 2860 2908 Size: 393 425 116 129 419 86 53 48 Cuts at: 2908 2918 3722 4319 5106 5374 1239 Size: 10 804 597 787 268 1524 Fragments arranged by size: 1524 804 787 597 425 419 393 268 129 116 86 53 48 10 EcoRI G'AATT_C Cuts at: 2968 3346 2968 Size: 378 5281 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2935 2938 2935 Size: 3 5656 FspI TGC'GCA Cuts at: 4165 4165 Size: 5659 HaeII r_GCGC'y Cuts at: 1678 2275 4806 1678 Size: 597 2531 2531 Fragments arranged by size: 2531 2531 597 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2002 2034 2246 2278 4300 Size: 32 135 32 430 32 212 32 2022 Cuts at: 4300 4332 4374 4406 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2022 430 212 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3003 3249 669 Size: 988 281 1065 246 3079 Fragments arranged by size: 3079 1065 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5659 HpaI GTT'AAC Cuts at: 3249 3249 Size: 5659 KpnI G_GTAC'C Cuts at: 2984 2984 Size: 5659 MfeI C'AATT_G Cuts at: 3258 3258 Size: 5659 MluI A'CGCG_T Cuts at: 5535 5535 Size: 5659 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2687 4773 4957 1305 Size: 1382 2086 184 2007 Fragments arranged by size: 2086 2007 1382 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2144 2231 2378 2594 10 Size: 54 1425 343 312 87 147 216 3075 Fragments arranged by size: 3075 1425 343 312 216 147 87 54 MslI CAynn'nnrTG Cuts at: 518 1166 2177 2354 2471 2747 2825 3617 3976 Size: 648 1011 177 117 276 78 792 359 Cuts at: 3976 4135 518 Size: 159 2042 Fragments arranged by size: 2042 1011 792 648 359 276 177 159 117 78 MspA1I CmG'CkG Cuts at: 1606 2077 2419 3075 3752 4900 5145 1606 Size: 471 342 656 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 656 471 342 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5107 NdeI CA'TA_TG Cuts at: 387 387 Size: 5659 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5659 NotI GC'GGCC_GC Cuts at: 3106 3106 Size: 5659 NspI r_CATG'y Cuts at: 1132 1229 2248 3017 4562 1132 Size: 97 1019 769 1545 2229 Fragments arranged by size: 2229 1545 1019 769 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5659 PciI A'CATG_T Cuts at: 4558 4558 Size: 5659 PflMI CCAn_nnn'nTGG Cuts at: 1108 2151 2809 1108 Size: 1043 658 3958 Fragments arranged by size: 3958 1043 658 PmeI GTTT'AAAC Cuts at: 2963 2963 Size: 5659 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3728 3760 5241 5273 3728 Size: 32 1481 32 4114 Fragments arranged by size: 4114 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5659 PsiI TTA'TAA Cuts at: 3229 3229 Size: 5659 PstI C_TGCA'G Cuts at: 830 3011 830 Size: 2181 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5659 PvuII CAG'CTG Cuts at: 2077 2077 Size: 5659 SacII CC_GC'GG Cuts at: 2420 2420 Size: 5659 SalI G'TCGA_C Cuts at: 1936 3001 1936 Size: 1065 4594 SapI GCTCTTCn'nnn_ Cuts at: 2090 2090 Size: 5659 SbfI CC_TGCA'GG Cuts at: 3011 3011 Size: 5659 ScaI AGT'ACT Cuts at: 3907 5619 3907 Size: 1712 3947 SfcI C'TryA_G Cuts at: 826 1045 3007 4142 4823 5014 826 Size: 219 1962 1135 681 191 1471 Fragments arranged by size: 1962 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5659 SmaI CCC'GGG Cuts at: 1872 2986 1872 Size: 1114 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3763 4664 4926 820 Size: 197 926 1820 901 262 1553 Fragments arranged by size: 1820 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3450 493 Size: 2957 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5659 SphI G_CATG'C Cuts at: 1132 2248 3017 1132 Size: 1116 769 3774 Fragments arranged by size: 3774 1116 769 SspI AAT'ATT Cuts at: 5 52 3583 5 Size: 47 3531 2081 Fragments arranged by size: 3531 2081 47 StuI AGG'CCT Cuts at: 1517 2717 1517 Size: 1200 4459 StyI C'CwwG_G Cuts at: 513 1065 3098 513 Size: 552 2033 3074 Fragments arranged by size: 3074 2033 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2282 2348 3686 3703 3856 4041 4486 Size: 1255 702 66 1338 17 153 185 445 Cuts at: 4486 325 Size: 1498 Fragments arranged by size: 1498 1338 1255 702 445 185 153 66 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3905 5617 96 Size: 274 80 33 51 1443 1928 1712 138 Fragments arranged by size: 1928 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2565 2597 5619 5651 2565 Size: 32 3022 32 2573 Fragments arranged by size: 3022 2573 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5659 XbaI T'CTAG_A Cuts at: 2995 2995 Size: 5659 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2284 2284 Size: 5659 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5659 XmaI C'CCGG_G Cuts at: 1870 2984 1870 Size: 1114 4545 XmnI GAAnn'nnTTC Cuts at: 2918 3788 2918 Size: 870 4789 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3467 276 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI