(Linear) (Six-base) MAPSORT of: KIEE5435.seq Check: 5325 from: 1 to: 954 KIEE5435.orf With 182 enzymes: SgfI * December 5, 2014 13:01 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 297 716 802 855 903 913 954 Size: 297 419 86 53 48 10 41 Fragments arranged by size: 419 297 86 53 48 41 10 AfeI AGC'GCT Cuts at: 0 268 954 Size: 268 686 AhdI GACnn_n'nnGTC Cuts at: 0 255 954 Size: 255 699 AleI CACnn'nnGTG Cuts at: 0 742 954 Size: 742 212 AlwNI CAG_nnn'CTG Cuts at: 0 804 954 Size: 804 150 ApaLI G'TGCA_C Cuts at: 0 751 954 Size: 751 203 BanII G_rGCy'C Cuts at: 0 412 954 Size: 412 542 BbsI GAAGACnn'nnnn_ Cuts at: 0 48 916 954 Size: 48 868 38 Fragments arranged by size: 868 48 38 BbvCI CC'TCA_GC Cuts at: 0 856 954 Size: 856 98 BglI GCCn_nnn'nGGC Cuts at: 0 283 416 434 954 Size: 283 133 18 520 Fragments arranged by size: 520 283 133 18 Bme1580I G_kGCm'C Cuts at: 0 755 954 Size: 755 199 BmrI ACTGGGnnnn_n' Cuts at: 0 792 954 Size: 792 162 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 52 168 954 Size: 52 116 786 Fragments arranged by size: 786 116 52 Bpu10I CC'TnA_GC Cuts at: 0 856 954 Size: 856 98 BsaAI yAC'GTr Cuts at: 0 369 954 Size: 369 585 BsaBI GATnn'nnATC Cuts at: 0 826 954 Size: 826 128 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 560 590 954 Size: 560 30 364 Fragments arranged by size: 560 364 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 628 637 954 Size: 628 9 317 Fragments arranged by size: 628 317 9 BseYI C'CCAG_C Cuts at: 0 117 263 624 954 Size: 117 146 361 330 Fragments arranged by size: 361 330 146 117 BsiEI CG_ry'CG Cuts at: 0 363 954 Size: 363 591 BsiHKAI G_wGCw'C Cuts at: 0 404 755 954 Size: 404 351 199 Fragments arranged by size: 404 351 199 BsmBI CGTCTCn'nnnn_ Cuts at: 0 207 563 954 Size: 207 356 391 Fragments arranged by size: 391 356 207 Bsp1286I G_dGCh'C Cuts at: 0 404 412 755 954 Size: 404 8 343 199 Fragments arranged by size: 404 343 199 8 BspHI T'CATG_A Cuts at: 0 404 954 Size: 404 550 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 134 260 954 Size: 134 126 694 Fragments arranged by size: 694 134 126 BstEII G'GTnAC_C Cuts at: 0 474 954 Size: 474 480 BstXI CCAn_nnnn'nTGG Cuts at: 0 597 954 Size: 597 357 BstYI r'GATC_y Cuts at: 0 209 518 683 954 Size: 209 309 165 271 Fragments arranged by size: 309 271 209 165 Bsu36I CC'TnA_GG Cuts at: 0 877 954 Size: 877 77 BtgI C'CryG_G Cuts at: 0 221 412 954 Size: 221 191 542 Fragments arranged by size: 542 221 191 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 258 954 Size: 258 696 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 533 568 954 Size: 533 35 386 Fragments arranged by size: 533 386 35 EaeI y'GGCC_r Cuts at: 0 137 224 245 371 482 587 668 954 Size: 137 87 21 126 111 105 81 286 Fragments arranged by size: 286 137 126 111 105 87 81 21 EarI CTCTTCn'nnn_ Cuts at: 0 85 187 954 Size: 85 102 767 Fragments arranged by size: 767 102 85 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 52 168 297 716 802 855 903 913 Size: 52 116 129 419 86 53 48 10 Cuts at: 913 954 Size: 41 Fragments arranged by size: 419 129 116 86 53 52 48 41 10 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 930 933 954 Size: 930 3 21 Fragments arranged by size: 930 21 3 HaeII r_GCGC'y Cuts at: 0 270 954 Size: 270 684 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 29 241 273 954 Size: 29 212 32 681 Fragments arranged by size: 681 212 32 29 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 682 954 Size: 682 272 MscI TGG'CCA Cuts at: 0 139 226 373 589 954 Size: 139 87 147 216 365 Fragments arranged by size: 365 216 147 139 87 MslI CAynn'nnrTG Cuts at: 0 172 349 466 742 820 954 Size: 172 177 117 276 78 134 Fragments arranged by size: 276 177 172 134 117 78 MspA1I CmG'CkG Cuts at: 0 72 414 954 Size: 72 342 540 Fragments arranged by size: 540 342 72 NspI r_CATG'y Cuts at: 0 243 954 Size: 243 711 PflMI CCAn_nnn'nTGG Cuts at: 0 146 804 954 Size: 146 658 150 Fragments arranged by size: 658 150 146 PvuII CAG'CTG Cuts at: 0 72 954 Size: 72 882 SacII CC_GC'GG Cuts at: 0 415 954 Size: 415 539 SapI GCTCTTCn'nnn_ Cuts at: 0 85 954 Size: 85 869 SphI G_CATG'C Cuts at: 0 243 954 Size: 243 711 StuI AGG'CCT Cuts at: 0 712 954 Size: 712 242 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 277 343 954 Size: 277 66 611 Fragments arranged by size: 611 277 66 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 560 592 954 Size: 560 32 362 Fragments arranged by size: 560 362 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 279 954 Size: 279 675 XmnI GAAnn'nnTTC Cuts at: 0 913 954 Size: 913 41 Enzymes that do cut: AcuI AfeI AhdI AleI AlwNI ApaLI BanII BbsI BbvCI BglI Bme1580I BmrI BpmI Bpu10I BsaAI BsaBI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BstEII BstXI BstYI Bsu36I BtgI BtgZI CspCI EaeI EarI Eco57MI FalI HaeII Hin4I MmeI MscI MslI MspA1I NspI PflMI PvuII SacII SapI SphI StuI TaqII TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AlfI AloI ApaI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsaI BsaHI BsaWI BsgI BsiWI BsmI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtsI ClaI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StyI SwaI TatI Tth111I XbaI XhoI XmaI ZraI