################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AlwNI ApaI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII AvrII BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco52I EcoNI EcoRV EcoT14I EcoT22I FbaI FseI FspI HapII Hin1I HincII HindII HindIII HinfI HpaI HpaII KasI KpnI KspI MaeI MamI MfeI MluI MroI MseI MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StyI SwaI TaqI TfiI Tru9I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccII 236 CGCG CG^CG AcsI 31 GAATTC [GA]^AATT[CT] AhdI 250 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 751 GTGCAC G^TGCAC Aor51HI 266 AGCGCT AGC^GCT ApaLI 751 GTGCAC G^TGCAC ApoI 31 GAATTC [GA]^AATT[CT] AspEI 250 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 508 GGATCC G^GATCC BanI 940 GGCACC G^G[CT][GA]CC BanII 408 GAGCCC G[GA]GC[CT]^C BpmI 147 CTGGAG CTGGAG(16/14) BsaAI 367 TACGTG [CT]AC^GT[GA] BsiEI 360 CGACCG CG[GA][CT]^CG BsmAI 202 GTCTC GTCTC(1/5) BsmFI 794 GTCCC GGGAC(10/14) BspHI 404 TCATGA T^CATGA BsrI 293 CCAGT ACTGG(1/-1) BstEII 474 GGTGACC G^GT[ACGT]ACC BstPI 474 GGTGACC G^GT[ACGT]ACC BstUI 236 CGCG CG^CG Eam1105I 250 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 266 AGCGCT AGC^GCT EcoO65I 474 GGTGACC G^GT[ACGT]ACC EcoRI 31 GAATTC G^AATTC FauI 234 CCCGC CCCGC(4/6) HaeII 266 AGCGCT [GA]GCGC^[CT] HgaI 191 GCGTC GACGC(5/10) MspA1I 581 CAGCTG C[AC]G^C[GT]G MvnI 236 CGCG CG^CG NspI 239 GCATGC [GA]CATG^[CT] PflMI 140 CCACCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 581 CAGCTG CAG^CTG RcaI 404 TCATGA T^CATGA SapI 78 GCTCTTC GCTCTTC(1/4) SfaNI 75 GATGC GCATC(5/9) SmlI 781 CTCAAG C^T[CT][GA]AG SphI 239 GCATGC GCATG^C StuI 710 AGGCCT AGG^CCT Tsp509I 32 AATT ^AATT Van91I 140 CCACCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 272 CCATCGCCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 235 CCGC CCGC(-3/-1) 363 CCGC AluI 316 AGCT AG^CT 582 AGCT AocI 876 CCTCAGG CC^T[ACGT]AGG 935 CCTCAGG AspHI 400 GTGCTC G[AT]GC[AT]^C 751 GTGCAC AvaII 793 GGTCC G^G[AT]CC 911 GGACC BbvCI 855 CCTCAGC CCTCAGC(-5/-2) 918 CCTCAGC BseRI 637 CTCCTC GAGGAG(10/8) 646 CTCCTC BsiHKAI 400 GTGCTC G[AT]GC[AT]^C 751 GTGCAC BspMI 125 ACCTGC ACCTGC(4/8) 251 ACCTGC Bsu36I 876 CCTCAGG CC^T[ACGT]AGG 935 CCTCAGG DraII 792 AGGTCCC [GA]G^G[ACGT]CC[CT] 910 AGGACCT DsaI 221 CCGTGG C^C[GA][CT]GG 412 CCACGG Eco81I 876 CCTCAGG CC^T[ACGT]AGG 935 CCTCAGG EcoO109I 792 AGGTCCC [GA]G^G[ACGT]CC[CT] 910 AGGACCT MaeII 368 ACGT A^CGT 398 ACGT MaeIII 475 GTGAC ^GT[ACGT]AC 812 GTGAC PpuMI 792 AGGTCCC [GA]G^G[AT]CC[CT] 910 AGGACCT Tsp45I 475 GTGAC ^GT[GC]AC 812 GTGAC TspRI 113 CACTG CA[GC]TG[ACGT][ACGT]^ 620 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AfaI AlwI BalI BbvI BglI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HphI ItaI Ksp632I MboI MboII MflI MluNI MnlI MscI MslI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TseI XhoII