################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI AspEI AviII AvrII BamHI BciVI BclI BfaI BfrI BlnI BsaBI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI Bst1107I BstZ17I ClaI CpoI DraI DraIII Eam1105I EarI Eco47III EcoRI EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI Ksp632I MaeI MamI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspI PacI PaeR7I PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfiI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tsp509I XbaI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 297 GGTACC G^GTACC AflIII 2195 ACGTGT A^C[GA][CT]GT AgeI 2086 ACCGGT A^CCGGT Asp700I 21 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 297 GGTACC G^GTACC AspHI 2176 GAGCTC G[AT]GC[AT]^C AspI 1137 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1798 TGGCCA TGG^CCA BbrPI 2029 CACGTG CAC^GTG BglII 326 AGATCT A^GATCT BsaAI 2029 CACGTG [CT]AC^GT[GA] BsaI 820 GGTCTC GGTCTC(1/5) BsiHKAI 2176 GAGCTC G[AT]GC[AT]^C BsmBI 1614 CGTCTC CGTCTC(1/5) BssHII 987 GCGCGC G^CGCGC BssSI 1712 CTCGTG CACGAG(-5/-1) BstAPI 1779 GCACTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 2095 TTCGAA TT^CGAA BstXI 1742 CCAGTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EagI 782 CGGCCG C^GGCCG EclXI 782 CGGCCG C^GGCCG Eco52I 782 CGGCCG C^GGCCG EcoRV 1399 GATATC GAT^ATC KpnI 297 GGTACC GGTAC^C KspI 1911 CCGCGG CCGC^GG MluNI 1798 TGGCCA TGG^CCA MscI 1798 TGGCCA TGG^CCA NcoI 1493 CCATGG C^CATGG NspV 2095 TTCGAA TT^CGAA PflFI 1137 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 2086 ACCGGT A^CCGGT PmaCI 2029 CACGTG CAC^GTG PmlI 2029 CACGTG CAC^GTG PstI 1789 CTGCAG CTGCA^G SacI 2176 GAGCTC GAGCT^C SacII 1911 CCGCGG CCGC^GG SfcI 1789 CTGCAG C^T[GA][CT]AG SfuI 2095 TTCGAA TT^CGAA SmlI 1423 CTCAAG C^T[CT][GA]AG TfiI 2133 GAATC G^A[AT]TC Tth111I 1137 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1251 CCAGAAGTGCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 21 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwI 1804 GATCC GGATC(4/5) 2163 GGATC AocI 346 CCTGAGG CC^T[ACGT]AGG 1111 CCTCAGG BcgI 573 CGAGAGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2103 CGACTTCGATGC BsaWI 705 ACCGGA [AT]^CCGG[AT] 2086 ACCGGT BsiEI 782 CGGCCG CG[GA][CT]^CG 1914 CGGTCG BsmAI 821 GTCTC GTCTC(1/5) 1615 GTCTC BstEII 682 GGTTACC G^GT[ACGT]ACC 2082 GGTCACC BstPI 682 GGTTACC G^GT[ACGT]ACC 2082 GGTCACC Bsu36I 346 CCTGAGG CC^T[ACGT]AGG 1111 CCTCAGG DrdI 1298 GACTGTACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2059 GACGAGGAGGTC Eco81I 346 CCTGAGG CC^T[ACGT]AGG 1111 CCTCAGG EcoNI 1377 CCTATCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1470 CCTGTATAAGG EcoO65I 682 GGTTACC G^GT[ACGT]ACC 2082 GGTCACC MaeII 2030 ACGT A^CGT 2195 ACGT MslI 501 CATAAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1767 CATCGAGGTG PflMI 388 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1086 CCAACAGCTGG StuI 1708 AGGCCT AGG^CCT 1734 AGGCCT Van91I 388 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1086 CCAACAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwNI ApaI AvaI AvaII BanI BanII BbeI BbsI BbvCI BbvI BcnI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfoI SgrAI SmaI StyI TaqI TseI Tsp45I TspRI TthHB8I XhoII XmaCI XmaI