(Circular) (Six-base) MAPSORT of: pFN21AE4214.seq Check: 4739 from: 1 to: 6910 pFN21AE4214 6910 bp With 182 enzymes: SgfI * March 28, 2012 13:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3618 3888 3618 Size: 270 6640 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4720 278 Size: 53 83 186 936 3184 2468 Fragments arranged by size: 3184 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4253 1937 Size: 2316 4594 Acc65I G'GTAC_C Cuts at: 2302 4231 2302 Size: 1929 4981 AclI AA'CG_TT Cuts at: 1563 5037 5410 1563 Size: 3474 373 3063 Fragments arranged by size: 3474 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4973 6357 6625 1632 Size: 3341 1384 268 1917 Fragments arranged by size: 3341 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6713 AflIII A'CryG_T Cuts at: 1203 4200 5809 6786 1203 Size: 2997 1609 977 1327 Fragments arranged by size: 2997 1609 1327 977 AgeI A'CCGG_T Cuts at: 4091 5741 4091 Size: 1650 5260 AhdI GACnn_n'nnGTC Cuts at: 5639 5639 Size: 6910 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6910 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3074 3394 1184 Size: 1890 320 4700 Fragments arranged by size: 4700 1890 320 AlwNI CAG_nnn'CTG Cuts at: 2378 2660 3124 3475 4653 6225 2378 Size: 282 464 351 1178 1572 3063 Fragments arranged by size: 3063 1572 1178 464 351 282 ApaI G_GGCC'C Cuts at: 2178 2489 2642 2730 2908 2178 Size: 311 153 88 178 6180 Fragments arranged by size: 6180 311 178 153 88 ApaLI G'TGCA_C Cuts at: 4967 6123 4967 Size: 1156 5754 ApoI r'AATT_y Cuts at: 1493 4219 4445 4597 6706 1493 Size: 2726 226 152 2109 1697 Fragments arranged by size: 2726 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5464 160 Size: 872 4432 1606 Fragments arranged by size: 4432 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6910 AvaI C'yCGr_G Cuts at: 1870 1943 2186 2386 2482 2559 2642 2677 2775 Size: 73 243 200 96 77 83 35 98 Cuts at: 2775 2828 3504 3546 3943 4235 1870 Size: 53 676 42 397 292 4545 Fragments arranged by size: 4545 676 397 292 243 200 98 96 83 77 73 53 42 35 BamHI G'GATC_C Cuts at: 4240 4607 4240 Size: 367 6543 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2302 2496 2535 2666 2779 Size: 325 217 627 515 194 39 131 113 Cuts at: 2779 3030 3050 3081 3241 3542 3703 4231 5686 Size: 251 20 31 160 301 161 528 1455 Cuts at: 5686 618 Size: 1842 Fragments arranged by size: 1842 1455 627 528 515 325 301 251 217 194 161 160 131 113 39 31 20 BanII G_rGCy'C Cuts at: 1391 2118 2139 2178 2204 2238 2322 2385 2489 Size: 727 21 39 26 34 84 63 104 Cuts at: 2489 2600 2642 2678 2730 2908 3183 3555 4185 Size: 111 42 36 52 178 275 372 630 Cuts at: 4185 4229 1391 Size: 44 4072 Fragments arranged by size: 4072 727 630 372 275 178 111 104 84 63 52 44 42 39 36 34 26 21 BbeI G_GCGC'C Cuts at: 2500 2539 3707 2500 Size: 39 1168 5703 Fragments arranged by size: 5703 1168 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2364 3064 4110 928 Size: 971 465 700 1046 3728 Fragments arranged by size: 3728 1046 971 700 465 BbvCI CC'TCA_GC Cuts at: 2277 2693 2807 3557 2277 Size: 416 114 750 5630 Fragments arranged by size: 5630 750 416 114 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2567 2601 4097 4131 5101 5135 1903 Size: 34 630 34 1496 34 970 34 3678 Fragments arranged by size: 3678 1496 970 630 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4802 6012 4802 Size: 1210 5700 BclI T'GATC_A Cuts at: 1548 1548 Size: 6910 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2046 2389 2553 Size: 107 122 71 631 605 374 343 164 Cuts at: 2553 3121 3712 4000 5521 136 Size: 568 591 288 1521 1525 Fragments arranged by size: 1525 1521 631 605 591 568 374 343 288 164 122 107 71 BglII A'GATC_T Cuts at: 2331 6905 2331 Size: 4574 2336 BlpI GC'TnA_GC Cuts at: 2404 3177 3322 4327 2404 Size: 773 145 1005 4987 Fragments arranged by size: 4987 1005 773 145 Bme1580I G_kGCm'C Cuts at: 1790 2178 2489 2642 2669 2730 2782 2908 3046 Size: 388 311 153 27 61 52 126 138 Cuts at: 3046 3053 3246 3545 3724 4971 6127 1790 Size: 7 193 299 179 1247 1156 2573 Fragments arranged by size: 2573 1247 1156 388 311 299 193 179 153 138 126 61 52 27 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6910 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2232 2322 4641 Size: 416 53 83 399 24 815 90 2319 Cuts at: 4641 5599 442 Size: 958 1753 Fragments arranged by size: 2319 1753 958 815 416 399 90 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6910 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2194 2302 3572 2194 Size: 108 1270 5532 Fragments arranged by size: 5532 1270 108 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2109 2164 2252 2355 2987 2993 3131 3512 Size: 870 55 88 103 632 6 138 381 Cuts at: 3512 3816 3988 4193 5570 1239 Size: 304 172 205 1377 2579 Fragments arranged by size: 2579 1377 870 632 381 304 205 172 138 103 88 55 6 Bpu10I CC'TnA_GC Cuts at: 2277 2379 2543 2693 2807 3557 2277 Size: 102 164 150 114 750 5630 Fragments arranged by size: 5630 750 164 150 114 102 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3413 5035 5900 6198 3413 Size: 1622 865 298 4125 Fragments arranged by size: 4125 1622 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2831 5573 882 Size: 617 1332 2742 2219 Fragments arranged by size: 2742 2219 1332 617 BsaAI yAC'GTr Cuts at: 493 1204 4036 4701 493 Size: 711 2832 665 2702 Fragments arranged by size: 2832 2702 711 665 BsaBI GATnn'nnATC Cuts at: 6904 6904 Size: 6910 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2079 2497 2536 3704 Size: 53 83 186 936 546 418 39 1168 Cuts at: 3704 4155 4717 5099 5734 275 Size: 451 562 382 635 1451 Fragments arranged by size: 1451 1168 936 635 562 546 451 418 382 186 83 53 39 BsaWI w'CCGG_w Cuts at: 2710 4091 5343 5741 6015 6162 6573 2710 Size: 1381 1252 398 274 147 411 3047 Fragments arranged by size: 3047 1381 1252 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6685 Fragments arranged by size: 6685 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3545 3613 3939 4082 1186 Size: 2359 68 326 143 4014 Fragments arranged by size: 4014 2359 326 143 68 BseYI C'CCAG_C Cuts at: 2098 2103 2117 2257 2719 2723 3468 6113 6609 Size: 5 14 140 462 4 745 2645 496 Cuts at: 6609 2098 Size: 2399 Fragments arranged by size: 2645 2399 745 496 462 140 14 5 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2190 2457 2798 3390 3767 3771 3860 Size: 573 454 267 341 592 377 4 89 Cuts at: 3860 1163 Size: 4213 Fragments arranged by size: 4213 592 573 454 377 341 267 89 4 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2790 3922 4360 5121 6149 1540 Size: 275 186 789 1132 438 761 1028 2301 Fragments arranged by size: 2301 1132 1028 789 761 438 275 186 BsiHKAI G_wGCw'C Cuts at: 4185 4971 5056 6127 4185 Size: 786 85 1071 4968 Fragments arranged by size: 4968 1071 786 85 BsmI GAATG_Cn' Cuts at: 4421 4514 4421 Size: 93 6817 BsmBI CGTCTCn'nnnn_ Cuts at: 3625 3625 Size: 6910 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2139 2178 2204 2238 2322 2385 Size: 399 328 21 39 26 34 84 63 Cuts at: 2385 2489 2600 2642 2669 2678 2730 2782 2908 Size: 104 111 42 27 9 52 52 126 Cuts at: 2908 3046 3053 3183 3246 3545 3555 3724 4185 Size: 138 7 130 63 299 10 169 461 Cuts at: 4185 4229 4971 5056 6127 1391 Size: 44 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 461 399 328 299 169 138 130 126 111 104 85 84 63 63 52 52 44 42 39 34 27 26 21 10 9 7 BspEI T'CCGG_A Cuts at: 6573 6573 Size: 6910 BspHI T'CATG_A Cuts at: 4799 6529 4799 Size: 1730 5180 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2229 3618 3798 3888 4265 844 Size: 1385 1389 180 90 377 3489 Fragments arranged by size: 3489 1389 1385 377 180 90 BsrBI CCG'CTC Cuts at: 1394 1823 3220 3226 3361 4797 1394 Size: 429 1397 6 135 1436 3507 Fragments arranged by size: 3507 1436 1397 429 135 6 BsrDI GCAATG_nn' Cuts at: 65 1248 5405 5579 65 Size: 1183 4157 174 1396 Fragments arranged by size: 4157 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2324 2647 2684 3033 3691 3959 4091 Size: 110 514 323 37 349 658 268 132 Cuts at: 4091 5554 5741 1700 Size: 1463 187 2869 Fragments arranged by size: 2869 1463 658 514 349 323 268 187 132 110 37 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6910 BssHII G'CGCG_C Cuts at: 1925 2992 1925 Size: 1067 5843 BssSI C'ACGA_G Cuts at: 3717 4970 5982 3717 Size: 1253 1012 4645 Fragments arranged by size: 4645 1253 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3790 794 Size: 2996 3914 BstBI TT'CG_AA Cuts at: 4101 5714 4101 Size: 1613 5297 BstEII G'GTnAC_C Cuts at: 1184 2687 4087 1184 Size: 1503 1400 4007 Fragments arranged by size: 4007 1503 1400 BstXI CCAn_nnnn'nTGG Cuts at: 3754 3754 Size: 6910 BstYI r'GATC_y Cuts at: 1972 2331 3808 4168 4240 4607 4992 5009 6461 Size: 359 1477 360 72 367 385 17 1452 Cuts at: 6461 6547 6559 6905 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1477 1452 385 367 360 359 346 86 72 17 12 Bsu36I CC'TnA_GG Cuts at: 2352 3117 2352 Size: 765 6145 BtgI C'CryG_G Cuts at: 513 1065 2004 3066 3498 3916 513 Size: 552 939 1062 432 418 3507 Fragments arranged by size: 3507 1062 939 552 432 418 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6171 Fragments arranged by size: 6171 683 56 BtsI GCAGTG_nn' Cuts at: 2918 3206 3336 3759 4051 4426 5220 5240 2918 Size: 288 130 423 292 375 794 20 4588 Fragments arranged by size: 4588 794 423 375 292 288 130 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6910 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3642 3677 529 Size: 35 3078 35 3762 Fragments arranged by size: 3762 3078 35 35 DraI TTT'AAA Cuts at: 4214 4214 Size: 6910 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3309 4070 5917 809 Size: 507 1993 761 1847 1802 Fragments arranged by size: 1993 1847 1802 761 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2092 2787 Size: 54 1425 50 275 18 177 85 695 Cuts at: 2787 2840 3099 3235 3663 3803 4357 5246 6875 Size: 53 259 136 428 140 554 889 1629 Cuts at: 6875 8 Size: 43 Fragments arranged by size: 1629 1425 889 695 554 428 275 259 177 140 136 85 54 53 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2787 4357 1537 Size: 275 975 1570 4090 Fragments arranged by size: 4090 1570 975 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4840 1353 Size: 3487 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5497 5871 6017 387 Size: 1064 138 3908 374 146 1280 Fragments arranged by size: 3908 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4183 4183 Size: 6910 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2109 2164 2252 2355 2987 2993 3131 Size: 393 477 55 88 103 632 6 138 Cuts at: 3131 3512 3816 3988 4193 4973 5570 6357 6625 Size: 381 304 172 205 780 597 787 268 Cuts at: 6625 1239 Size: 1524 Fragments arranged by size: 1524 787 780 632 597 477 393 381 304 268 205 172 138 103 88 55 6 EcoNI CCTnn'n_nnAGG Cuts at: 3386 3479 3386 Size: 93 6817 EcoO109I rG'GnC_Cy Cuts at: 2154 2174 2175 2294 2419 2486 2555 2638 2639 Size: 20 1 119 125 67 69 83 1 Cuts at: 2639 2657 2727 2765 2866 2904 3013 3391 3508 Size: 18 70 38 101 38 109 378 117 Cuts at: 3508 3929 2154 Size: 421 5135 Fragments arranged by size: 5135 421 378 125 119 117 109 101 83 70 69 67 38 38 20 18 1 1 EcoRI G'AATT_C Cuts at: 4219 4597 4219 Size: 378 6532 EcoRV GAT'ATC Cuts at: 3406 3406 Size: 6910 FspI TGC'GCA Cuts at: 5416 5416 Size: 6910 HaeII r_GCGC'y Cuts at: 1678 2110 2500 2539 2719 2849 3283 3707 6057 Size: 432 390 39 180 130 434 424 2350 Cuts at: 6057 1678 Size: 2531 Fragments arranged by size: 2531 2350 434 432 424 390 180 130 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2690 2722 3998 4030 5551 Size: 32 135 32 1118 32 1276 32 1521 Cuts at: 5551 5583 5625 5657 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1521 1276 1118 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4254 4500 669 Size: 988 281 2316 246 3079 Fragments arranged by size: 3079 2316 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6910 HpaI GTT'AAC Cuts at: 4500 4500 Size: 6910 KasI G'GCGC_C Cuts at: 2496 2535 3703 2496 Size: 39 1168 5703 Fragments arranged by size: 5703 1168 39 KpnI G_GTAC'C Cuts at: 2306 4235 2306 Size: 1929 4981 MfeI C'AATT_G Cuts at: 4509 4509 Size: 6910 MluI A'CGCG_T Cuts at: 6786 6786 Size: 6910 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2055 3129 6024 6208 1305 Size: 750 1074 2895 184 2007 Fragments arranged by size: 2895 2007 1074 750 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3805 10 Size: 54 1425 343 1973 3115 Fragments arranged by size: 3115 1973 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2510 3776 4868 5227 5386 518 Size: 648 1344 1266 1092 359 159 2042 Fragments arranged by size: 2042 1344 1266 1092 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2276 2408 2723 3097 3176 3326 3374 3561 Size: 670 132 315 374 79 150 48 187 Cuts at: 3561 3886 3918 4326 5003 6151 6396 1606 Size: 325 32 408 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 670 408 374 325 315 245 187 150 132 79 48 32 NaeI GCC'GGC Cuts at: 2326 2649 3693 3961 2326 Size: 323 1044 268 5275 Fragments arranged by size: 5275 1044 323 268 NarI GG'CG_CC Cuts at: 2497 2536 3704 2497 Size: 39 1168 5703 Fragments arranged by size: 5703 1168 39 NcoI C'CATG_G Cuts at: 513 1065 2004 3498 513 Size: 552 939 1494 3925 Fragments arranged by size: 3925 1494 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6910 NgoMIV G'CCGG_C Cuts at: 2324 2647 3691 3959 2324 Size: 323 1044 268 5275 Fragments arranged by size: 5275 1044 323 268 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6910 NotI GC'GGCC_GC Cuts at: 4357 4357 Size: 6910 NspI r_CATG'y Cuts at: 1132 1229 4268 5813 1132 Size: 97 3039 1545 2229 Fragments arranged by size: 3039 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2862 3046 1348 Size: 1514 184 5212 Fragments arranged by size: 5212 1514 184 PciI A'CATG_T Cuts at: 5809 5809 Size: 6910 PflMI CCAn_nnn'nTGG Cuts at: 1108 2399 3097 1108 Size: 1291 698 4921 Fragments arranged by size: 4921 1291 698 PfoI T'CCnGG_A Cuts at: 3600 4164 3600 Size: 564 6346 PmeI GTTT'AAAC Cuts at: 4214 4214 Size: 6910 PmlI CAC'GTG Cuts at: 4036 4036 Size: 6910 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4979 5011 6492 6524 4979 Size: 32 1481 32 5365 Fragments arranged by size: 5365 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2154 2765 2866 3013 3391 3508 2154 Size: 611 101 147 378 117 5556 Fragments arranged by size: 5556 611 378 147 117 101 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6910 PsiI TTA'TAA Cuts at: 4480 4480 Size: 6910 PspOMI G'GGCC_C Cuts at: 2174 2485 2638 2726 2904 2174 Size: 311 153 88 178 6180 Fragments arranged by size: 6180 311 178 153 88 PstI C_TGCA'G Cuts at: 830 3798 4262 830 Size: 2968 464 3478 Fragments arranged by size: 3478 2968 464 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6910 PvuII CAG'CTG Cuts at: 2276 2408 2723 3097 3176 3561 2276 Size: 132 315 374 79 385 5625 Fragments arranged by size: 5625 385 374 315 132 79 SacI G_AGCT'C Cuts at: 4185 4185 Size: 6910 SacII CC_GC'GG Cuts at: 3919 3919 Size: 6910 SalI G'TCGA_C Cuts at: 1936 4252 1936 Size: 2316 4594 SanDI GG'GwC_CC Cuts at: 2154 2765 3013 3508 2154 Size: 611 248 495 5556 Fragments arranged by size: 5556 611 495 248 SbfI CC_TGCA'GG Cuts at: 4262 4262 Size: 6910 ScaI AGT'ACT Cuts at: 5158 6870 5158 Size: 1712 5198 SfcI C'TryA_G Cuts at: 826 1045 3794 4258 5393 6074 6265 826 Size: 219 2749 464 1135 681 191 1471 Fragments arranged by size: 2749 1471 1135 681 464 219 191 SfoI GGC'GCC Cuts at: 2498 2537 3705 2498 Size: 39 1168 5703 Fragments arranged by size: 5703 1168 39 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6910 SgrAI Cr'CCGG_yG Cuts at: 1700 2324 3959 4091 1700 Size: 624 1635 132 4519 Fragments arranged by size: 4519 1635 624 132 SmaI CCC'GGG Cuts at: 1872 2388 2484 2561 2644 2777 3506 4237 1872 Size: 516 96 77 83 133 729 731 4545 Fragments arranged by size: 4545 731 729 516 133 96 83 77 SmlI C'TyrA_G Cuts at: 820 1017 1943 3428 5014 5915 6177 820 Size: 197 926 1485 1586 901 262 1553 Fragments arranged by size: 1586 1553 1485 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4701 493 Size: 4208 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6910 SphI G_CATG'C Cuts at: 1132 4268 1132 Size: 3136 3774 SrfI GCCC'GGGC Cuts at: 2777 2777 Size: 6910 SspI AAT'ATT Cuts at: 5 52 4834 5 Size: 47 4782 2081 Fragments arranged by size: 4782 2081 47 StuI AGG'CCT Cuts at: 1517 3715 3741 1517 Size: 2198 26 4686 Fragments arranged by size: 4686 2198 26 StyI C'CwwG_G Cuts at: 513 1065 2004 2082 2431 3498 4349 513 Size: 552 939 78 349 1067 851 3074 Fragments arranged by size: 3074 1067 939 851 552 349 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2112 2242 2249 2752 3482 4937 4954 Size: 1255 532 130 7 503 730 1455 17 Cuts at: 4954 5107 5292 5737 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1455 1255 730 532 503 445 185 153 130 17 7 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5156 6868 96 Size: 274 80 33 51 1443 3179 1712 138 Fragments arranged by size: 3179 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6870 6902 6870 Size: 32 6878 Tth111I GACn'n_nGTC Cuts at: 1316 3145 1316 Size: 1829 5081 XbaI T'CTAG_A Cuts at: 4246 4246 Size: 6910 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3263 3263 Size: 6910 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6910 XmaI C'CCGG_G Cuts at: 1870 2386 2482 2559 2642 2775 3504 4235 1870 Size: 516 96 77 83 133 729 731 4545 Fragments arranged by size: 4545 731 729 516 133 96 83 77 XmnI GAAnn'nnTTC Cuts at: 2030 5039 2030 Size: 3009 3901 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4718 276 Size: 53 83 186 936 3184 2468 Fragments arranged by size: 3184 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SapI SexAI SfiI SwaI