(Linear) (Six-base) MAPSORT of: KIEE4214.seq Check: 7827 from: 1 to: 2205 KIEE4214.orf With 182 enzymes: SgfI * March 22, 2012 17:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1613 1883 2205 Size: 1613 270 322 Fragments arranged by size: 1613 322 270 Acc65I G'GTAC_C Cuts at: 0 297 2205 Size: 297 1908 AflIII A'CryG_T Cuts at: 0 2195 2205 Size: 2195 10 AgeI A'CCGG_T Cuts at: 0 2086 2205 Size: 2086 119 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1069 1389 2205 Size: 1069 320 816 Fragments arranged by size: 1069 816 320 AlwNI CAG_nnn'CTG Cuts at: 0 373 655 1119 1470 2205 Size: 373 282 464 351 735 Fragments arranged by size: 735 464 373 351 282 ApaI G_GGCC'C Cuts at: 0 173 484 637 725 903 2205 Size: 173 311 153 88 178 1302 Fragments arranged by size: 1302 311 178 173 153 88 AvaI C'yCGr_G Cuts at: 0 181 381 477 554 637 672 770 823 Size: 181 200 96 77 83 35 98 53 Cuts at: 823 1499 1541 1938 2205 Size: 676 42 397 267 Fragments arranged by size: 676 397 267 200 181 98 96 83 77 53 42 35 BanI G'GyrC_C Cuts at: 0 297 491 530 661 774 1025 1045 1076 Size: 297 194 39 131 113 251 20 31 Cuts at: 1076 1236 1537 1698 2205 Size: 160 301 161 507 Fragments arranged by size: 507 301 297 251 194 161 160 131 113 39 31 20 BanII G_rGCy'C Cuts at: 0 113 134 173 199 233 317 380 484 Size: 113 21 39 26 34 84 63 104 Cuts at: 484 595 637 673 725 903 1178 1550 2180 Size: 111 42 36 52 178 275 372 630 Cuts at: 2180 2205 Size: 25 Fragments arranged by size: 630 372 275 178 113 111 104 84 63 52 42 39 36 34 26 25 21 BbeI G_GCGC'C Cuts at: 0 495 534 1702 2205 Size: 495 39 1168 503 Fragments arranged by size: 1168 503 495 39 BbsI GAAGACnn'nnnn_ Cuts at: 0 359 1059 2105 2205 Size: 359 700 1046 100 Fragments arranged by size: 1046 700 359 100 BbvCI CC'TCA_GC Cuts at: 0 272 688 802 1552 2205 Size: 272 416 114 750 653 Fragments arranged by size: 750 653 416 272 114 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 562 596 2092 2126 2205 Size: 562 34 1496 34 79 Fragments arranged by size: 1496 562 79 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 41 384 548 1116 1707 1995 2205 Size: 41 343 164 568 591 288 210 Fragments arranged by size: 591 568 343 288 210 164 41 BglII A'GATC_T Cuts at: 0 326 2205 Size: 326 1879 BlpI GC'TnA_GC Cuts at: 0 399 1172 1317 2205 Size: 399 773 145 888 Fragments arranged by size: 888 773 399 145 Bme1580I G_kGCm'C Cuts at: 0 173 484 637 664 725 777 903 1041 Size: 173 311 153 27 61 52 126 138 Cuts at: 1041 1048 1241 1540 1719 2205 Size: 7 193 299 179 486 Fragments arranged by size: 486 311 299 193 179 173 153 138 126 61 52 27 7 BmrI ACTGGGnnnn_n' Cuts at: 0 227 317 2205 Size: 227 90 1888 Fragments arranged by size: 1888 227 90 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 189 297 1567 2205 Size: 189 108 1270 638 Fragments arranged by size: 1270 638 189 108 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 104 159 247 350 982 988 1126 1507 Size: 104 55 88 103 632 6 138 381 Cuts at: 1507 1811 1983 2188 2205 Size: 304 172 205 17 Fragments arranged by size: 632 381 304 205 172 138 104 103 88 55 17 6 Bpu10I CC'TnA_GC Cuts at: 0 272 374 538 688 802 1552 2205 Size: 272 102 164 150 114 750 653 Fragments arranged by size: 750 653 272 164 150 114 102 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1408 2205 Size: 1408 797 BsaI GGTCTCn'nnnn_ Cuts at: 0 826 2205 Size: 826 1379 BsaAI yAC'GTr Cuts at: 0 2031 2205 Size: 2031 174 BsaHI Gr'CG_yC Cuts at: 0 74 492 531 1699 2150 2205 Size: 74 418 39 1168 451 55 Fragments arranged by size: 1168 451 418 74 55 39 BsaWI w'CCGG_w Cuts at: 0 705 2086 2205 Size: 705 1381 119 Fragments arranged by size: 1381 705 119 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1540 1608 1934 2077 2205 Size: 1540 68 326 143 128 Fragments arranged by size: 1540 326 143 128 68 BseYI C'CCAG_C Cuts at: 0 93 98 112 252 714 718 1463 2205 Size: 93 5 14 140 462 4 745 742 Fragments arranged by size: 745 742 462 140 93 14 5 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 185 452 793 1385 1762 1766 1855 2205 Size: 185 267 341 592 377 4 89 350 Fragments arranged by size: 592 377 350 341 267 185 89 4 BsiEI CG_ry'CG Cuts at: 0 785 1917 2205 Size: 785 1132 288 Fragments arranged by size: 1132 785 288 BsiHKAI G_wGCw'C Cuts at: 0 2180 2205 Size: 2180 25 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1620 2205 Size: 1620 585 Bsp1286I G_dGCh'C Cuts at: 0 113 134 173 199 233 317 380 484 Size: 113 21 39 26 34 84 63 104 Cuts at: 484 595 637 664 673 725 777 903 1041 Size: 111 42 27 9 52 52 126 138 Cuts at: 1041 1048 1178 1241 1540 1550 1719 2180 2205 Size: 7 130 63 299 10 169 461 25 Fragments arranged by size: 461 299 169 138 130 126 113 111 104 84 63 63 52 52 42 39 34 27 26 25 21 10 9 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 224 1613 1793 1883 2205 Size: 224 1389 180 90 322 Fragments arranged by size: 1389 322 224 180 90 BsrBI CCG'CTC Cuts at: 0 1215 1221 1356 2205 Size: 1215 6 135 849 Fragments arranged by size: 1215 849 135 6 BsrFI r'CCGG_y Cuts at: 0 319 642 679 1028 1686 1954 2086 2205 Size: 319 323 37 349 658 268 132 119 Fragments arranged by size: 658 349 323 319 268 132 119 37 BssHII G'CGCG_C Cuts at: 0 987 2205 Size: 987 1218 BssSI C'ACGA_G Cuts at: 0 1712 2205 Size: 1712 493 BstAPI GCAn_nnn'nTGC Cuts at: 0 1785 2205 Size: 1785 420 BstBI TT'CG_AA Cuts at: 0 2096 2205 Size: 2096 109 BstEII G'GTnAC_C Cuts at: 0 682 2082 2205 Size: 682 1400 123 Fragments arranged by size: 1400 682 123 BstXI CCAn_nnnn'nTGG Cuts at: 0 1749 2205 Size: 1749 456 BstYI r'GATC_y Cuts at: 0 326 1803 2163 2205 Size: 326 1477 360 42 Fragments arranged by size: 1477 360 326 42 Bsu36I CC'TnA_GG Cuts at: 0 347 1112 2205 Size: 347 765 1093 Fragments arranged by size: 1093 765 347 BtgI C'CryG_G Cuts at: 0 1061 1493 1911 2205 Size: 1061 432 418 294 Fragments arranged by size: 1061 432 418 294 BtsI GCAGTG_nn' Cuts at: 0 913 1201 1331 1754 2046 2205 Size: 913 288 130 423 292 159 Fragments arranged by size: 913 423 292 288 159 130 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1637 1672 2205 Size: 1637 35 533 Fragments arranged by size: 1637 533 35 DrdI GACnn_nn'nnGTC Cuts at: 0 1304 2065 2205 Size: 1304 761 140 Fragments arranged by size: 1304 761 140 EaeI y'GGCC_r Cuts at: 0 2 87 782 835 1094 1230 1658 1798 Size: 2 85 695 53 259 136 428 140 Cuts at: 1798 2205 Size: 407 Fragments arranged by size: 695 428 407 259 140 136 85 53 2 EagI C'GGCC_G Cuts at: 0 782 2205 Size: 782 1423 EcoICRI GAG'CTC Cuts at: 0 2178 2205 Size: 2178 27 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 104 159 247 350 982 988 1126 1507 Size: 104 55 88 103 632 6 138 381 Cuts at: 1507 1811 1983 2188 2205 Size: 304 172 205 17 Fragments arranged by size: 632 381 304 205 172 138 104 103 88 55 17 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 1381 1474 2205 Size: 1381 93 731 Fragments arranged by size: 1381 731 93 EcoO109I rG'GnC_Cy Cuts at: 0 149 169 170 289 414 481 550 633 Size: 149 20 1 119 125 67 69 83 Cuts at: 633 634 652 722 760 861 899 1008 1386 Size: 1 18 70 38 101 38 109 378 Cuts at: 1386 1503 1924 2205 Size: 117 421 281 Fragments arranged by size: 421 378 281 149 125 119 117 109 101 83 70 69 67 38 38 20 18 1 1 EcoRV GAT'ATC Cuts at: 0 1401 2205 Size: 1401 804 HaeII r_GCGC'y Cuts at: 0 105 495 534 714 844 1278 1702 2205 Size: 105 390 39 180 130 434 424 503 Fragments arranged by size: 503 434 424 390 180 130 105 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 685 717 1993 2025 2205 Size: 685 32 1276 32 180 Fragments arranged by size: 1276 685 180 32 32 KasI G'GCGC_C Cuts at: 0 491 530 1698 2205 Size: 491 39 1168 507 Fragments arranged by size: 1168 507 491 39 KpnI G_GTAC'C Cuts at: 0 301 2205 Size: 301 1904 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 50 1124 2205 Size: 50 1074 1081 Fragments arranged by size: 1081 1074 50 MscI TGG'CCA Cuts at: 0 1800 2205 Size: 1800 405 MslI CAynn'nnrTG Cuts at: 0 505 1771 2205 Size: 505 1266 434 Fragments arranged by size: 1266 505 434 MspA1I CmG'CkG Cuts at: 0 271 403 718 1092 1171 1321 1369 1556 Size: 271 132 315 374 79 150 48 187 Cuts at: 1556 1881 1913 2205 Size: 325 32 292 Fragments arranged by size: 374 325 315 292 271 187 150 132 79 48 32 NaeI GCC'GGC Cuts at: 0 321 644 1688 1956 2205 Size: 321 323 1044 268 249 Fragments arranged by size: 1044 323 321 268 249 NarI GG'CG_CC Cuts at: 0 492 531 1699 2205 Size: 492 39 1168 506 Fragments arranged by size: 1168 506 492 39 NcoI C'CATG_G Cuts at: 0 1493 2205 Size: 1493 712 NgoMIV G'CCGG_C Cuts at: 0 319 642 1686 1954 2205 Size: 319 323 1044 268 251 Fragments arranged by size: 1044 323 319 268 251 PasI CC'CwG_GG Cuts at: 0 857 1041 2205 Size: 857 184 1164 Fragments arranged by size: 1164 857 184 PflMI CCAn_nnn'nTGG Cuts at: 0 394 1092 2205 Size: 394 698 1113 Fragments arranged by size: 1113 698 394 PfoI T'CCnGG_A Cuts at: 0 1595 2159 2205 Size: 1595 564 46 Fragments arranged by size: 1595 564 46 PmlI CAC'GTG Cuts at: 0 2031 2205 Size: 2031 174 PpuMI rG'GwC_Cy Cuts at: 0 149 760 861 1008 1386 1503 2205 Size: 149 611 101 147 378 117 702 Fragments arranged by size: 702 611 378 149 147 117 101 PspOMI G'GGCC_C Cuts at: 0 169 480 633 721 899 2205 Size: 169 311 153 88 178 1306 Fragments arranged by size: 1306 311 178 169 153 88 PstI C_TGCA'G Cuts at: 0 1793 2205 Size: 1793 412 PvuII CAG'CTG Cuts at: 0 271 403 718 1092 1171 1556 2205 Size: 271 132 315 374 79 385 649 Fragments arranged by size: 649 385 374 315 271 132 79 SacI G_AGCT'C Cuts at: 0 2180 2205 Size: 2180 25 SacII CC_GC'GG Cuts at: 0 1914 2205 Size: 1914 291 SanDI GG'GwC_CC Cuts at: 0 149 760 1008 1503 2205 Size: 149 611 248 495 702 Fragments arranged by size: 702 611 495 248 149 SfcI C'TryA_G Cuts at: 0 1789 2205 Size: 1789 416 SfoI GGC'GCC Cuts at: 0 493 532 1700 2205 Size: 493 39 1168 505 Fragments arranged by size: 1168 505 493 39 SgrAI Cr'CCGG_yG Cuts at: 0 319 1954 2086 2205 Size: 319 1635 132 119 Fragments arranged by size: 1635 319 132 119 SmaI CCC'GGG Cuts at: 0 383 479 556 639 772 1501 2205 Size: 383 96 77 83 133 729 704 Fragments arranged by size: 729 704 383 133 96 83 77 SmlI C'TyrA_G Cuts at: 0 1423 2205 Size: 1423 782 SrfI GCCC'GGGC Cuts at: 0 772 2205 Size: 772 1433 StuI AGG'CCT Cuts at: 0 1710 1736 2205 Size: 1710 26 469 Fragments arranged by size: 1710 469 26 StyI C'CwwG_G Cuts at: 0 77 426 1493 2205 Size: 77 349 1067 712 Fragments arranged by size: 1067 712 349 77 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 107 237 244 747 1477 2205 Size: 107 130 7 503 730 728 Fragments arranged by size: 730 728 503 130 107 7 Tth111I GACn'n_nGTC Cuts at: 0 1140 2205 Size: 1140 1065 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1258 2205 Size: 1258 947 XmaI C'CCGG_G Cuts at: 0 381 477 554 637 770 1499 2205 Size: 381 96 77 83 133 729 706 Fragments arranged by size: 729 706 381 133 96 83 77 XmnI GAAnn'nnTTC Cuts at: 0 25 2205 Size: 25 2180 Enzymes that do cut: AarI Acc65I AflIII AgeI AlfI AlwNI ApaI AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI DrdI EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV PasI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SacII SanDI SfcI SfoI SgrAI SmaI SmlI SrfI StuI StyI TaqII Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII AccI AclI AcuI AfeI AflII AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BmgBI BmtI BsaBI BsaXI BsiWI BsmI BspEI BspHI BsrDI BsrGI BstZ17I BtgZI ClaI DraI DraIII EarI EciI EcoRI FalI FseI FspI FspAI HincII HindIII HpaI MfeI MluI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI SgfI SnaBI SpeI SphI SspI SwaI TatI TstI XbaI XhoI ZraI