################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BfaI BfrI BglII BlnI BsaAI BsaHI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BspHI BspMI BsrBI BsrDI BsrFI BsrI BssHII BssSI Bst1107I BstAPI BstXI BstZ17I Bsu36I CfoI Cfr10I CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI MaeI MfeI MluI MluNI MscI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tru9I Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 69 enzymes) # ################################### AccII 11 CGCG CG^CG AccIII 704 TCCGGA T^CCGGA AcsI 199 AAATTC [GA]^AATT[CT] AflIII 122 ACATGT A^C[GA][CT]GT AlwI 229 GATCC GGATC(4/5) ApoI 199 AAATTC [GA]^AATT[CT] Asp700I 318 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 411 GGACC G^G[AT]CC BanI 46 GGTGCC G^G[CT][GA]CC BbsI 100 GAAGAC GAAGAC(2/6) BbvI 132 GCAGC GCAGC(8/12) BciVI 520 GGATAC GTATCC(6/5) BclI 540 TGATCA T^GATCA BglI 49 GCCCGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 364 GCTAAGC GC^T[ACGT]AGC BmyI 47 GTGCCC G[AGT]GC[ACT]^C BpmI 313 CTGGAG CTGGAG(16/14) Bpu1102I 364 GCTAAGC GC^T[ACGT]AGC BpuAI 100 GAAGAC GAAGAC(2/6) BsaBI 447 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 161 GAGACC GGTCTC(1/5) BsaWI 704 TCCGGA [AT]^CCGG[AT] BseAI 704 TCCGGA T^CCGGA BsmAI 161 GAGAC GTCTC(1/5) BsmFI 410 GGGAC GGGAC(10/14) Bsp1286I 47 GTGCCC G[AGT]GC[ACT]^C Bsp1407I 377 TGTACA T^GTACA BspDI 214 ATCGAT AT^CGAT BspEI 704 TCCGGA T^CCGGA BspLU11I 122 ACATGT A^CATGT BsrGI 377 TGTACA T^GTACA BstBI 142 TTCGAA TT^CGAA BstEII 624 GGTGACC G^GT[ACGT]ACC BstPI 624 GGTGACC G^GT[ACGT]ACC BstUI 11 CGCG CG^CG BstYI 228 AGATCC [GA]^GATC[CT] CelII 364 GCTAAGC GC^T[ACGT]AGC ClaI 214 ATCGAT AT^CGAT EcoO65I 624 GGTGACC G^GT[ACGT]ACC EcoT14I 371 CCAAGG C^C[AT][AT]GG FauI 50 CCCGC CCCGC(4/6) FbaI 540 TGATCA T^GATCA Fnu4HI 132 GCAGC GC^[ACGT]GC ItaI 132 GCAGC GC^[ACGT]GC KspI 10 CCGCGG CCGC^GG MaeII 500 ACGT A^CGT MamI 447 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 228 AGATCC [GA]^GATC[CT] MroI 704 TCCGGA T^CCGGA MvnI 11 CGCG CG^CG NspI 122 ACATGT [GA]CATG^[CT] NspV 142 TTCGAA TT^CGAA PflMI 564 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 410 GGGACCC [GA]G^G[AT]CC[CT] PstI 721 CTGCAG CTGCA^G PvuII 133 CAGCTG CAG^CTG SacII 10 CCGCGG CCGC^GG SapI 187 GAAGAGC GCTCTTC(1/4) SfcI 721 CTGCAG C^T[GA][CT]AG SfuI 142 TTCGAA TT^CGAA SmlI 283 CTCAAG C^T[CT][GA]AG SspBI 377 TGTACA T^GTACA StuI 389 AGGCCT AGG^CCT StyI 371 CCAAGG C^C[AT][AT]GG TseI 132 GCAGC G^C[AT]GC TspRI 168 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 564 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 228 AGATCC [GA]^GATC[CT] XmnI 318 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 253 GTAC GT^AC 378 GTAC AlwNI 352 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG 565 CAGACCCTG AvaI 83 CCCGGG C^[CT]CG[GA]G 221 CCCGGG BseRI 25 GAGGAG GAGGAG(10/8) 398 GAGGAG BsoBI 83 CCCGGG C^[CT]CG[GA]G 221 CCCGGG DdeI 136 CTGAG C^T[ACGT]AG 365 CTAAG DpnII 229 GATC ^GATC 541 GATC DsaI 10 CCGCGG C^C[GA][CT]GG 509 CCACGG MaeIII 73 GTCAC ^GT[ACGT]AC 625 GTGAC MboI 229 GATC ^GATC 541 GATC MspA1I 10 CCGCGG C[AC]G^C[GT]G 133 CAGCTG NdeII 229 GATC ^GATC 541 GATC RsaI 253 GTAC GT^AC 378 GTAC Sau3AI 229 GATC ^GATC 541 GATC SfaNI 109 GATGC GCATC(5/9) 490 GATGC SmaI 83 CCCGGG CCC^GGG 221 CCCGGG Tsp45I 73 GTCAC ^GT[GC]AC 625 GTGAC Tsp509I 179 AATT ^AATT 200 AATT XmaCI 83 CCCGGG C^CCGGG 221 CCCGGG XmaI 83 CCCGGG C^CCGGG 221 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AciI AluI BcnI BsaJI BsiYI BslI BssKI BstF5I BstNI Cac8I Cfr13I DraII EarI EcoO109I EcoRII FokI HaeIII HapII HinfI HpaII HphI Ksp632I MboII MnlI MspI MvaI MwoI NciI NlaIII NlaIV Sau96I ScrFI TaqI TfiI TthHB8I