(Circular) (Six-base) MAPSORT of: pFN21AE1735.seq Check: 6860 from: 1 to: 5431 pFN21AE1735 5431 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3241 278 Size: 53 83 186 936 1705 2468 Fragments arranged by size: 2468 1705 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2774 1937 Size: 837 4594 Acc65I G'GTAC_C Cuts at: 2752 2752 Size: 5431 AclI AA'CG_TT Cuts at: 1563 3558 3931 1563 Size: 1995 373 3063 Fragments arranged by size: 3063 1995 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3494 4878 5146 1632 Size: 1862 1384 268 1917 Fragments arranged by size: 1917 1862 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5234 AflIII A'CryG_T Cuts at: 1203 2127 4330 5307 1203 Size: 924 2203 977 1327 Fragments arranged by size: 2203 1327 977 924 AgeI A'CCGG_T Cuts at: 4262 4262 Size: 5431 AhdI GACnn_n'nnGTC Cuts at: 4160 4160 Size: 5431 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5431 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5431 AlwNI CAG_nnn'CTG Cuts at: 2362 2575 3174 4746 2362 Size: 213 599 1572 3047 Fragments arranged by size: 3047 1572 599 213 ApaLI G'TGCA_C Cuts at: 3488 4644 3488 Size: 1156 4275 ApoI r'AATT_y Cuts at: 1493 2204 2740 2966 3118 5227 1493 Size: 711 536 226 152 2109 1697 Fragments arranged by size: 2109 1697 711 536 226 152 AseI AT'TA_AT Cuts at: 160 1032 3985 160 Size: 872 2953 1606 Fragments arranged by size: 2953 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5431 AvaI C'yCGr_G Cuts at: 1870 1943 2088 2226 2756 1870 Size: 73 145 138 530 4545 Fragments arranged by size: 4545 530 145 138 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2705 2738 2705 Size: 33 5398 BamHI G'GATC_C Cuts at: 2761 3128 2761 Size: 367 5064 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2051 2752 4207 618 Size: 325 217 627 264 701 1455 1842 Fragments arranged by size: 1842 1455 701 627 325 264 217 BanII G_rGCy'C Cuts at: 1391 2750 1391 Size: 1359 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2112 928 Size: 971 213 4247 Fragments arranged by size: 4247 971 213 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3622 3656 1903 Size: 34 1685 34 3678 Fragments arranged by size: 3678 1685 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2519 3323 4533 2519 Size: 804 1210 3417 Fragments arranged by size: 3417 1210 804 BclI T'GATC_A Cuts at: 1548 2545 1548 Size: 997 4434 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2060 4042 136 Size: 107 122 71 631 605 388 1982 1525 Fragments arranged by size: 1982 1525 631 605 388 122 107 71 BglII A'GATC_T Cuts at: 5426 5426 Size: 5431 BlpI GC'TnA_GC Cuts at: 2370 2848 2370 Size: 478 4953 Bme1580I G_kGCm'C Cuts at: 1790 2056 3492 4648 1790 Size: 266 1436 1156 2573 Fragments arranged by size: 2573 1436 1156 266 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5431 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3162 4120 442 Size: 416 53 83 399 24 1745 958 1753 Fragments arranged by size: 1753 1745 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5431 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2339 4091 1239 Size: 1100 1752 2579 Fragments arranged by size: 2579 1752 1100 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2273 3556 4421 4719 2273 Size: 1283 865 298 2985 Fragments arranged by size: 2985 1283 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2160 4094 882 Size: 617 661 1934 2219 Fragments arranged by size: 2219 1934 661 617 BsaAI yAC'GTr Cuts at: 493 1204 3222 493 Size: 711 2018 2702 Fragments arranged by size: 2702 2018 711 BsaBI GATnn'nnATC Cuts at: 2456 5425 2456 Size: 2969 2462 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3238 3620 4255 275 Size: 53 83 186 936 1705 382 635 1451 Fragments arranged by size: 1705 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2709 3864 4262 4536 4683 5094 2709 Size: 1155 398 274 147 411 3046 Fragments arranged by size: 3046 1155 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5206 Fragments arranged by size: 5206 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2045 2418 1186 Size: 859 373 4199 Fragments arranged by size: 4199 859 373 BseYI C'CCAG_C Cuts at: 4634 5130 4634 Size: 496 4935 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4858 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2881 3642 4670 1540 Size: 275 186 880 761 1028 2301 Fragments arranged by size: 2301 1028 880 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3492 3577 4648 3492 Size: 85 1071 4275 Fragments arranged by size: 4275 1071 85 BsmI GAATG_Cn' Cuts at: 2942 3035 2942 Size: 93 5338 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2056 2750 3492 3577 4648 1391 Size: 399 266 694 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 694 399 266 85 BspEI T'CCGG_A Cuts at: 2709 5094 2709 Size: 2385 3046 BspHI T'CATG_A Cuts at: 3320 5050 3320 Size: 1730 3701 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2719 2786 844 Size: 1875 67 3489 Fragments arranged by size: 3489 1875 67 BsrBI CCG'CTC Cuts at: 1394 1823 3318 1394 Size: 429 1495 3507 Fragments arranged by size: 3507 1495 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3926 4100 65 Size: 1183 2678 174 1396 Fragments arranged by size: 2678 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4075 4262 1700 Size: 110 2265 187 2869 Fragments arranged by size: 2869 2265 187 110 BsrGI T'GTAC_A Cuts at: 96 2382 96 Size: 2286 3145 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5431 BssSI C'ACGA_G Cuts at: 3491 4503 3491 Size: 1012 4419 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5431 BstBI TT'CG_AA Cuts at: 2148 4235 2148 Size: 2087 3344 BstEII G'GTnAC_C Cuts at: 1184 2629 1184 Size: 1445 3986 BstYI r'GATC_y Cuts at: 1972 2233 2761 3128 3513 3530 4982 5068 5080 Size: 261 528 367 385 17 1452 86 12 Cuts at: 5080 5426 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 528 385 367 346 261 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2015 2514 513 Size: 552 950 499 3430 Fragments arranged by size: 3430 950 552 499 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2515 487 Size: 683 56 1289 3403 Fragments arranged by size: 3403 1289 683 56 BtsI GCAGTG_nn' Cuts at: 2172 2947 3741 3761 2172 Size: 775 794 20 3842 Fragments arranged by size: 3842 794 775 20 ClaI AT'CG_AT Cuts at: 1554 2220 1554 Size: 666 4765 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5396 DraI TTT'AAA Cuts at: 2735 2735 Size: 5431 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4438 809 Size: 507 3122 1802 Fragments arranged by size: 3122 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2878 3767 5396 Size: 54 1425 50 275 18 1048 889 1629 Cuts at: 5396 8 Size: 43 Fragments arranged by size: 1629 1425 1048 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2878 1537 Size: 275 1066 4090 Fragments arranged by size: 4090 1066 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2022 2187 2333 2588 3361 1353 Size: 669 165 146 255 773 3423 Fragments arranged by size: 3423 773 669 255 165 146 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2653 4018 4392 4538 387 Size: 1064 138 1064 1365 374 146 1280 Fragments arranged by size: 1365 1280 1064 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2339 3494 4091 4878 5146 1239 Size: 393 707 1155 597 787 268 1524 Fragments arranged by size: 1524 1155 787 707 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2335 2359 2416 2335 Size: 24 57 5350 Fragments arranged by size: 5350 57 24 EcoRI G'AATT_C Cuts at: 2740 3118 2740 Size: 378 5053 FspI TGC'GCA Cuts at: 3937 3937 Size: 5431 HaeII r_GCGC'y Cuts at: 1678 4578 1678 Size: 2900 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4072 4104 4146 4178 1373 Size: 32 135 32 2500 32 42 32 2626 Fragments arranged by size: 2626 2500 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2775 3021 669 Size: 988 281 837 246 3079 Fragments arranged by size: 3079 988 837 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5431 HpaI GTT'AAC Cuts at: 3021 3021 Size: 5431 KpnI G_GTAC'C Cuts at: 2756 2756 Size: 5431 MfeI C'AATT_G Cuts at: 3030 3030 Size: 5431 MluI A'CGCG_T Cuts at: 5307 5307 Size: 5431 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4545 4729 1305 Size: 3240 184 2007 Fragments arranged by size: 3240 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3609 Fragments arranged by size: 3609 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3389 3748 3907 518 Size: 648 2223 359 159 2042 Fragments arranged by size: 2223 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2017 2140 2847 3524 4672 4917 1606 Size: 411 123 707 677 1148 245 2120 Fragments arranged by size: 2120 1148 707 677 411 245 123 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4879 NdeI CA'TA_TG Cuts at: 387 387 Size: 5431 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5431 NotI GC'GGCC_GC Cuts at: 2878 2878 Size: 5431 NspI r_CATG'y Cuts at: 1132 1229 2131 2789 4334 1132 Size: 97 902 658 1545 2229 Fragments arranged by size: 2229 1545 902 658 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5431 PciI A'CATG_T Cuts at: 2127 4330 2127 Size: 2203 3228 PflMI CCAn_nnn'nTGG Cuts at: 1108 2575 1108 Size: 1467 3964 PfoI T'CCnGG_A Cuts at: 2187 2187 Size: 5431 PmeI GTTT'AAAC Cuts at: 2735 2735 Size: 5431 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3500 3532 5013 5045 3500 Size: 32 1481 32 3886 Fragments arranged by size: 3886 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2416 2416 Size: 5431 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5431 PsiI TTA'TAA Cuts at: 3001 3001 Size: 5431 PstI C_TGCA'G Cuts at: 830 2730 2783 830 Size: 1900 53 3478 Fragments arranged by size: 3478 1900 53 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5431 PvuII CAG'CTG Cuts at: 2140 2140 Size: 5431 SacII CC_GC'GG Cuts at: 2018 2018 Size: 5431 SalI G'TCGA_C Cuts at: 1936 2773 1936 Size: 837 4594 SanDI GG'GwC_CC Cuts at: 2416 2416 Size: 5431 SapI GCTCTTCn'nnn_ Cuts at: 2187 2187 Size: 5431 SbfI CC_TGCA'GG Cuts at: 2783 2783 Size: 5431 ScaI AGT'ACT Cuts at: 3679 5391 3679 Size: 1712 3719 SfcI C'TryA_G Cuts at: 826 1045 2726 2779 3914 4595 4786 826 Size: 219 1681 53 1135 681 191 1471 Fragments arranged by size: 1681 1471 1135 681 219 191 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5431 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5431 SmaI CCC'GGG Cuts at: 1872 2090 2228 2758 1872 Size: 218 138 530 4545 Fragments arranged by size: 4545 530 218 138 SmlI C'TyrA_G Cuts at: 820 1017 1943 2288 3535 4436 4698 820 Size: 197 926 345 1247 901 262 1553 Fragments arranged by size: 1553 1247 926 901 345 262 197 SnaBI TAC'GTA Cuts at: 493 3222 493 Size: 2729 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5431 SphI G_CATG'C Cuts at: 1132 2789 1132 Size: 1657 3774 SspI AAT'ATT Cuts at: 5 52 3355 5 Size: 47 3303 2081 Fragments arranged by size: 3303 2081 47 StuI AGG'CCT Cuts at: 1517 2396 1517 Size: 879 4552 StyI C'CwwG_G Cuts at: 513 1065 2376 2870 513 Size: 552 1311 494 3074 Fragments arranged by size: 3074 1311 552 494 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3458 3475 3628 3813 4258 325 Size: 1255 1878 17 153 185 445 1498 Fragments arranged by size: 1878 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2382 3677 5389 Size: 274 80 33 51 1443 405 1295 1712 Cuts at: 5389 96 Size: 138 Fragments arranged by size: 1712 1443 1295 405 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2463 2495 2527 5391 5423 2463 Size: 32 32 2864 32 2471 Fragments arranged by size: 2864 2471 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5431 XbaI T'CTAG_A Cuts at: 2767 2767 Size: 5431 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5431 XmaI C'CCGG_G Cuts at: 1870 2088 2226 2756 1870 Size: 218 138 530 4545 Fragments arranged by size: 4545 530 218 138 XmnI GAAnn'nnTTC Cuts at: 2327 3560 2327 Size: 1233 4198 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3239 276 Size: 53 83 186 936 1705 2468 Fragments arranged by size: 2468 1705 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SexAI SfiI SfoI SrfI SwaI XcmI