(Linear) (Six-base) MAPSORT of: KIEE1735.seq Check: 8420 from: 1 to: 726 KIEE1735.orf With 182 enzymes: SgfI * July 15, 2010 18:46 .. AflIII A'CryG_T Cuts at: 0 122 726 Size: 122 604 AlwNI CAG_nnn'CTG Cuts at: 0 357 570 726 Size: 357 213 156 Fragments arranged by size: 357 213 156 ApoI r'AATT_y Cuts at: 0 199 726 Size: 199 527 AvaI C'yCGr_G Cuts at: 0 83 221 726 Size: 83 138 505 Fragments arranged by size: 505 138 83 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 700 726 Size: 700 26 BanI G'GyrC_C Cuts at: 0 46 726 Size: 46 680 BbsI GAAGACnn'nnnn_ Cuts at: 0 107 726 Size: 107 619 BciVI GTATCCnnnnn_n' Cuts at: 0 514 726 Size: 514 212 BclI T'GATC_A Cuts at: 0 540 726 Size: 540 186 BglI GCCn_nnn'nGGC Cuts at: 0 55 726 Size: 55 671 BlpI GC'TnA_GC Cuts at: 0 365 726 Size: 365 361 Bme1580I G_kGCm'C Cuts at: 0 51 726 Size: 51 675 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 334 726 Size: 334 392 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 268 726 Size: 268 458 BsaI GGTCTCn'nnnn_ Cuts at: 0 155 726 Size: 155 571 BsaBI GATnn'nnATC Cuts at: 0 451 726 Size: 451 275 BsaWI w'CCGG_w Cuts at: 0 704 726 Size: 704 22 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 40 413 726 Size: 40 373 313 Fragments arranged by size: 373 313 40 Bsp1286I G_dGCh'C Cuts at: 0 51 726 Size: 51 675 BspEI T'CCGG_A Cuts at: 0 704 726 Size: 704 22 BsrGI T'GTAC_A Cuts at: 0 377 726 Size: 377 349 BstBI TT'CG_AA Cuts at: 0 143 726 Size: 143 583 BstEII G'GTnAC_C Cuts at: 0 624 726 Size: 624 102 BstYI r'GATC_y Cuts at: 0 228 726 Size: 228 498 BtgI C'CryG_G Cuts at: 0 10 509 726 Size: 10 499 217 Fragments arranged by size: 499 217 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 510 726 Size: 510 216 BtsI GCAGTG_nn' Cuts at: 0 167 726 Size: 167 559 ClaI AT'CG_AT Cuts at: 0 215 726 Size: 215 511 EarI CTCTTCn'nnn_ Cuts at: 0 17 182 328 583 726 Size: 17 165 146 255 143 Fragments arranged by size: 255 165 146 143 17 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 648 726 Size: 648 78 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 334 726 Size: 334 392 EcoO109I rG'GnC_Cy Cuts at: 0 330 354 411 726 Size: 330 24 57 315 Fragments arranged by size: 330 315 57 24 MspA1I CmG'CkG Cuts at: 0 12 135 726 Size: 12 123 591 Fragments arranged by size: 591 123 12 NspI r_CATG'y Cuts at: 0 126 726 Size: 126 600 PciI A'CATG_T Cuts at: 0 122 726 Size: 122 604 PflMI CCAn_nnn'nTGG Cuts at: 0 570 726 Size: 570 156 PfoI T'CCnGG_A Cuts at: 0 182 726 Size: 182 544 PpuMI rG'GwC_Cy Cuts at: 0 411 726 Size: 411 315 PstI C_TGCA'G Cuts at: 0 725 726 Size: 725 1 PvuII CAG'CTG Cuts at: 0 135 726 Size: 135 591 SacII CC_GC'GG Cuts at: 0 13 726 Size: 13 713 SanDI GG'GwC_CC Cuts at: 0 411 726 Size: 411 315 SapI GCTCTTCn'nnn_ Cuts at: 0 182 726 Size: 182 544 SfcI C'TryA_G Cuts at: 0 721 726 Size: 721 5 SmaI CCC'GGG Cuts at: 0 85 223 726 Size: 85 138 503 Fragments arranged by size: 503 138 85 SmlI C'TyrA_G Cuts at: 0 283 726 Size: 283 443 StuI AGG'CCT Cuts at: 0 391 726 Size: 391 335 StyI C'CwwG_G Cuts at: 0 371 726 Size: 371 355 TatI w'GTAC_w Cuts at: 0 377 726 Size: 377 349 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 458 490 522 726 Size: 458 32 32 204 Fragments arranged by size: 458 204 32 32 XmaI C'CCGG_G Cuts at: 0 83 221 726 Size: 83 138 505 Fragments arranged by size: 505 138 83 XmnI GAAnn'nnTTC Cuts at: 0 322 726 Size: 322 404 Enzymes that do cut: AflIII AlwNI ApoI AvaI BaeI BanI BbsI BciVI BclI BglI BlpI Bme1580I BpmI BpuEI BsaI BsaBI BsaWI BseRI Bsp1286I BspEI BsrGI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI EarI EciI Eco57MI EcoO109I MspA1I NspI PciI PflMI PfoI PpuMI PstI PvuII SacII SanDI SapI SfcI SmaI SmlI StuI StyI TatI TstI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BamHI BanII BbeI BbvCI BcgI BfrBI BglII BmgBI BmrI BmtI BplI Bpu10I BsaAI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstXI BstZ17I Bsu36I CspCI DraI DraIII DrdI EaeI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI XcmI XhoI ZraI