################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlpI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### Asp700I 31 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 115 GTGCTC G[AT]GC[AT]^C AvaI 118 CTCGGG C^[CT]CG[GA]G AvaII 625 GGTCC G^G[AT]CC AvrII 765 CCTAGG C^CTAGG BamHI 638 GGATCC G^GATCC BanII 896 GGGCTC G[GA]GC[CT]^C BfaI 766 CTAG C^TAG BlnI 765 CCTAGG C^CTAGG BsaAI 674 TACGTG [CT]AC^GT[GA] BsiHKAI 115 GTGCTC G[AT]GC[AT]^C BsmAI 742 GTCTC GTCTC(1/5) BsoBI 118 CTCGGG C^[CT]CG[GA]G Bsp1407I 830 TGTACA T^GTACA BspHI 404 TCATGA T^CATGA BsrGI 830 TGTACA T^GTACA BstXI 98 CCATGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 638 GGATCC [GA]^GATC[CT] EcoRI 462 GAATTC G^AATTC EcoT14I 765 CCTAGG C^C[AT][AT]GG HindIII 477 AAGCTT A^AGCTT MaeI 766 CTAG C^TAG MaeII 675 ACGT A^CGT MflI 638 GGATCC [GA]^GATC[CT] MwoI 346 GCTGTGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC RcaI 404 TCATGA T^CATGA SexAI 104 ACCTGGT A^CC[AT]GGT SfcI 867 CTACAG C^T[GA][CT]AG SspBI 830 TGTACA T^GTACA StyI 765 CCTAGG C^C[AT][AT]GG TfiI 123 GAATC G^A[AT]TC XhoII 638 GGATCC [GA]^GATC[CT] XmnI 31 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 31 GAATTT [GA]^AATT[CT] 462 GAATTC AlwI 639 GATCC GGATC(4/5) 638 GGATC ApoI 31 GAATTT [GA]^AATT[CT] 462 GAATTC BmyI 115 GTGCTC G[AGT]GC[ACT]^C 896 GGGCTC BsaJI 412 CCTCGG C^C[ACGT][ACGT]GG 765 CCTAGG BsgI 388 CTGCAC GTGCAG(16/14) 504 CTGCAC BsmFI 45 GGGAC GGGAC(10/14) 626 GTCCC Bsp1286I 115 GTGCTC G[AGT]GC[ACT]^C 896 GGGCTC Cac8I 283 GCAGGC GC[ACGT]^[ACGT]GC 615 GCTGGC Cfr13I 625 GGTCC G^G[ACGT]CC 762 GGGCC DpnII 361 GATC ^GATC 639 GATC HphI 275 TCACC GGTGA(8/7) 293 TCACC MaeIII 109 GTCAC ^GT[ACGT]AC 471 GTCAC MboI 361 GATC ^GATC 639 GATC MboII 39 TCTTC GAAGA(8/7) 182 TCTTC NdeII 361 GATC ^GATC 639 GATC Sau3AI 361 GATC ^GATC 639 GATC Sau96I 625 GGTCC G^G[ACGT]CC 762 GGGCC Tsp45I 109 GTCAC ^GT[GC]AC 471 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AfaI AluI BalI BbvI BsiYI BslI BssKI BstF5I BstNI DdeI EaeI EcoRII Fnu4HI FokI HaeIII HinfI ItaI MluNI MnlI MscI MseI MslI MvaI NlaIII NlaIV PleI RsaI ScrFI SfaNI Tru9I TseI Tsp509I TspRI