################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaLI AscI Asp718I AspEI AspHI AspI AviII AvrII BamHI BanI BbeI BbrPI BbsI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MroI MunI MvnI MwoI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I Van91I XbaI XcmI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AciI 363 CCGC CCGC(-3/-1) AcyI 610 GGCGTC G[GA]^CG[CT]C AocI 873 CCTGAGG CC^T[ACGT]AGG ApaI 896 GGGCCC GGGCC^C AseI 580 ATTAAT AT^TAAT AsnI 580 ATTAAT AT^TAAT AvaI 472 CTCGAG C^[CT]CG[GA]G AvaII 440 GGTCC G^G[AT]CC BanII 896 GGGCCC G[GA]GC[CT]^C BbvCI 686 CCTCAGC CCTCAGC(-5/-2) BbvI 787 GCAGC GCAGC(8/12) BcnI 119 CCGGG CC^[GC]GG BmyI 896 GGGCCC G[AGT]GC[ACT]^C BsaHI 610 GGCGTC G[GA]^CG[CT]C BsaI 475 GAGACC GGTCTC(1/5) BsmFI 232 GTCCC GGGAC(10/14) BsoBI 472 CTCGAG C^[CT]CG[GA]G Bsp1286I 896 GGGCCC G[AGT]GC[ACT]^C Bsp1407I 830 TGTACA T^GTACA BspHI 404 TCATGA T^CATGA BspMI 125 ACCTGC ACCTGC(4/8) BsrGI 830 TGTACA T^GTACA BstXI 414 CCAGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 873 CCTGAGG CC^T[ACGT]AGG DpnII 538 GATC ^GATC DsaI 836 CCATGG C^C[GA][CT]GG EarI 891 GAAGAG CTCTTC(1/4) Eco81I 873 CCTGAGG CC^T[ACGT]AGG EcoT14I 836 CCATGG C^C[AT][AT]GG Fnu4HI 787 GCAGC GC^[ACGT]GC HapII 119 CCGG C^CGG HgaI 611 GCGTC GACGC(5/10) Hin1I 610 GGCGTC G[GA]^CG[CT]C HpaII 119 CCGG C^CGG ItaI 787 GCAGC GC^[ACGT]GC Ksp632I 891 GAAGAG CTCTTC(1/4) MboI 538 GATC ^GATC MspA1I 689 CAGCTG C[AC]G^C[GT]G MspI 119 CCGG C^CGG NciI 119 CCGGG CC^[GC]GG NcoI 836 CCATGG C^CATGG NdeII 538 GATC ^GATC NspI 21 ACATGC [GA]CATG^[CT] PaeR7I 472 CTCGAG C^TCGAG PpuMI 439 GGGTCCT [GA]G^G[AT]CC[CT] PshBI 580 ATTAAT AT^TAAT PvuII 689 CAGCTG CAG^CTG RcaI 404 TCATGA T^CATGA Sau3AI 538 GATC ^GATC SmlI 472 CTCGAG C^T[CT][GA]AG SspBI 830 TGTACA T^GTACA SspI 639 AATATT AAT^ATT StyI 836 CCATGG C^C[AT][AT]GG TaqI 473 TCGA T^CGA TfiI 66 GATTC G^A[AT]TC TseI 787 GCAGC G^C[AT]GC TthHB8I 473 TCGA T^CGA XhoI 472 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### Asp700I 264 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 855 GAACCCCTTC BalI 140 TGGCCA TGG^CCA 371 TGGCCA BfaI 106 CTAG C^TAG 803 CTAG BseRI 37 CTCCTC GAGGAG(10/8) 910 CTCCTC BsgI 388 CTGCAC GTGCAG(16/14) 504 CTGCAC BsrI 607 ACTGG ACTGG(1/-1) 634 ACTGG BstEII 741 GGTCACC G^GT[ACGT]ACC 840 GGTCACC BstF5I 135 CATCC GGATG(2/0) 322 GGATG BstPI 741 GGTCACC G^GT[ACGT]ACC 840 GGTCACC Cac8I 283 GCAGGC GC[ACGT]^[ACGT]GC 558 GCTCGC EaeI 140 TGGCCA [CT]^GGCC[GA] 371 TGGCCA EcoO65I 741 GGTCACC G^GT[ACGT]ACC 840 GGTCACC FokI 135 CATCC GGATG(9/13) 322 GGATG MaeI 106 CTAG C^TAG 803 CTAG MaeII 58 ACGT A^CGT 587 ACGT MluNI 140 TGGCCA TGG^CCA 371 TGGCCA MscI 140 TGGCCA TGG^CCA 371 TGGCCA MseI 581 TTAA T^TAA 932 TTAA NdeI 144 CATATG CA^TATG 278 CATATG PleI 154 GACTC GAGTC(4/5) 908 GACTC PstI 785 CTGCAG CTGCA^G 947 CTGCAG SfaNI 323 GATGC GCATC(5/9) 449 GCATC Tru9I 581 TTAA T^TAA 932 TTAA XmnI 264 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 855 GAACCCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AcsI AfaI AluI ApoI BsaJI BsiYI BslI BsmAI BssKI BstNI Cfr13I DdeI DraII EcoO109I EcoRII HaeIII HinfI HphI MaeIII MboII MnlI MslI MvaI NlaIII NlaIV RsaI Sau96I ScrFI SfcI Tsp45I Tsp509I TspRI