Restriction map for
pFN21AE9564
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BcgI BciVI BclI BfaI
BfrI BglII BlnI BsaBI BsaWI
BseAI BsgI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BsrDI
BsrI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI Eam1105I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HinfI HpaI HphI
KpnI MaeI MamI MfeI MluI
MroI MseI MunI NcoI NdeI
NheI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmeI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI TfiI Tru9I
Tsp509I Tth111I TthHB8I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AflIII 74 ACACGT A^C[GA][CT]GT
AocI 731 CCTCAGG CC^T[ACGT]AGG
Aor51HI 401 AGCGCT AGC^GCT
ApaI 13 GGGCCC GGGCC^C
AviII 1172 TGCGCA TGC^GCA
BbsI 352 GTCTTC GAAGAC(2/6)
BbvCI 1194 CCTCAGC CCTCAGC(-5/-2)
BlpI 669 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 669 GCTCAGC GC^T[ACGT]AGC
BpuAI 352 GTCTTC GAAGAC(2/6)
BsaI 444 GGTCTC GGTCTC(1/5)
BseRI 643 CTCCTC GAGGAG(10/8)
BsmAI 445 GTCTC GTCTC(1/5)
BspMI 149 ACCTGC ACCTGC(4/8)
BstF5I 1053 CATCC GGATG(2/0)
BstYI 1031 AGATCC [GA]^GATC[CT]
Bsu36I 731 CCTCAGG CC^T[ACGT]AGG
CelII 669 GCTCAGC GC^T[ACGT]AGC
EarI 616 CTCTTC CTCTTC(1/4)
Eco47III 401 AGCGCT AGC^GCT
Eco81I 731 CCTCAGG CC^T[ACGT]AGG
FokI 1053 CATCC GGATG(9/13)
FspI 1172 TGCGCA TGC^GCA
Ksp632I 616 CTCTTC CTCTTC(1/4)
MaeIII 415 GTCAC ^GT[ACGT]AC
MflI 1031 AGATCC [GA]^GATC[CT]
NotI 420 GCGGCCGC GC^GGCCGC
NspI 49 ACATGC [GA]CATG^[CT]
SfaNI 920 GCATC GCATC(5/9)
Tsp45I 415 GTCAC ^GT[GC]AC
XcmI 623 CCAGCCTCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1031 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AlwI 267 GATCC GGATC(4/5)
1032 GATCC
AlwNI 598 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
1030 CAGATCCTG
AspHI 860 GAGCTC G[AT]GC[AT]^C
972 GAGCTC
BalI 230 TGGCCA TGG^CCA
242 TGGCCA
BbrPI 262 CACGTG CAC^GTG
555 CACGTG
BpmI 927 CTGGAG CTGGAG(16/14)
969 CTGGAG
BsaAI 262 CACGTG [CT]AC^GT[GA]
555 CACGTG
BsiHKAI 860 GAGCTC G[AT]GC[AT]^C
972 GAGCTC
BsmFI 67 GTCCC GGGAC(10/14)
578 GTCCC
Bsp1407I 293 TGTACA T^GTACA
1049 TGTACA
BsrFI 316 GCCGGC [GA]^CCGG[CT]
746 GCCGGC
BsrGI 293 TGTACA T^GTACA
1049 TGTACA
BstAPI 554 GCACGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
601 GCACTGCTTGC
Cfr10I 316 GCCGGC [GA]^CCGG[CT]
746 GCCGGC
DpnII 267 GATC ^GATC
1032 GATC
DraIII 555 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
871 CACGACGTG
MboI 267 GATC ^GATC
1032 GATC
MluNI 230 TGGCCA TGG^CCA
242 TGGCCA
MscI 230 TGGCCA TGG^CCA
242 TGGCCA
MslI 390 CATGGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
906 CATGGTGGTG
NaeI 316 GCCGGC GCC^GGC
746 GCCGGC
NdeII 267 GATC ^GATC
1032 GATC
NgoMIV 316 GCCGGC G^CCGGC
746 GCCGGC
PmaCI 262 CACGTG CAC^GTG
555 CACGTG
PmlI 262 CACGTG CAC^GTG
555 CACGTG
PpuMI 65 GGGTCCC [GA]G^G[AT]CC[CT]
576 GGGTCCC
PstI 541 CTGCAG CTGCA^G
985 CTGCAG
SacI 860 GAGCTC GAGCT^C
972 GAGCTC
Sau3AI 267 GATC ^GATC
1032 GATC
SfcI 541 CTGCAG C^T[GA][CT]AG
985 CTGCAG
SspBI 293 TGTACA T^GTACA
1049 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BglI BmyI BsaHI
BsaJI BsiEI BsiYI BslI BsoBI
Bsp1286I BsrBI BssHII BssKI BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DraII DsaI EaeI EagI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
ItaI KasI KspI MaeII MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NarI NciI NlaIII NlaIV
PflMI RsaI SacII Sau96I ScrFI
SfiI SfoI SmaI StyI TseI
TspRI Van91I XmaCI XmaI