(Circular) (Six-base) MAPSORT of: pFN21AE9564.seq Check: 4956 from: 1 to: 5911 pFN21AE9564 5911 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3721 278 Size: 53 83 186 936 2185 2468 Fragments arranged by size: 2468 2185 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3254 1937 Size: 1317 4594 Acc65I G'GTAC_C Cuts at: 3232 3232 Size: 5911 AclI AA'CG_TT Cuts at: 1563 4038 4411 1563 Size: 2475 373 3063 Fragments arranged by size: 3063 2475 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3974 5358 5626 1632 Size: 2342 1384 268 1917 Fragments arranged by size: 2342 1917 1384 268 AfeI AGC'GCT Cuts at: 2408 2408 Size: 5911 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5714 AflIII A'CryG_T Cuts at: 1203 2079 4810 5787 1203 Size: 876 2731 977 1327 Fragments arranged by size: 2731 1327 977 876 AgeI A'CCGG_T Cuts at: 4742 4742 Size: 5911 AhdI GACnn_n'nnGTC Cuts at: 4640 4640 Size: 5911 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5911 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5911 AlwNI CAG_nnn'CTG Cuts at: 2608 3040 3654 5226 2608 Size: 432 614 1572 3293 Fragments arranged by size: 3293 1572 614 432 ApaI G_GGCC'C Cuts at: 2022 2022 Size: 5911 ApaLI G'TGCA_C Cuts at: 3968 5124 3968 Size: 1156 4755 ApoI r'AATT_y Cuts at: 1493 3220 3446 3598 5707 1493 Size: 1727 226 152 2109 1697 Fragments arranged by size: 2109 1727 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4465 160 Size: 872 3433 1606 Fragments arranged by size: 3433 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 AvaI C'yCGr_G Cuts at: 1870 1943 2275 2554 2587 3106 3236 1870 Size: 73 332 279 33 519 130 4545 Fragments arranged by size: 4545 519 332 279 130 73 33 BamHI G'GATC_C Cuts at: 3241 3608 3241 Size: 367 5544 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2062 2141 2598 3232 4687 Size: 325 217 627 275 79 457 634 1455 Cuts at: 4687 618 Size: 1842 Fragments arranged by size: 1842 1455 634 627 457 325 275 217 79 BanII G_rGCy'C Cuts at: 1391 2012 2022 2869 2939 2981 3230 1391 Size: 621 10 847 70 42 249 4072 Fragments arranged by size: 4072 847 621 249 70 42 10 BbeI G_GCGC'C Cuts at: 2066 2145 2602 2066 Size: 79 457 5375 Fragments arranged by size: 5375 457 79 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2350 928 Size: 971 451 4489 Fragments arranged by size: 4489 971 451 BbvCI CC'TCA_GC Cuts at: 3200 3200 Size: 5911 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4102 4136 1903 Size: 34 2165 34 3678 Fragments arranged by size: 3678 2165 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3803 5013 3803 Size: 1210 4701 BclI T'GATC_A Cuts at: 1548 1548 Size: 5911 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2040 2327 2486 Size: 107 122 71 631 605 368 287 159 Cuts at: 2486 2495 3164 4522 136 Size: 9 669 1358 1525 Fragments arranged by size: 1525 1358 669 631 605 368 287 159 122 107 71 9 BglII A'GATC_T Cuts at: 5906 5906 Size: 5911 BlpI GC'TnA_GC Cuts at: 2675 3328 2675 Size: 653 5258 Bme1580I G_kGCm'C Cuts at: 1790 2022 2375 2561 3972 5128 1790 Size: 232 353 186 1411 1156 2573 Fragments arranged by size: 2573 1411 1156 353 232 186 BmgBI CAC'GTC Cuts at: 1318 2082 2881 1318 Size: 764 799 4348 Fragments arranged by size: 4348 799 764 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3642 4600 442 Size: 416 53 83 399 24 2225 958 1753 Fragments arranged by size: 2225 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5911 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3187 3187 Size: 5911 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2953 2995 4571 1239 Size: 1714 42 1576 2579 Fragments arranged by size: 2579 1714 1576 42 Bpu10I CC'TnA_GC Cuts at: 2030 3200 2030 Size: 1170 4741 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4036 4901 5199 4036 Size: 865 298 4748 Fragments arranged by size: 4748 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2455 4574 882 Size: 617 956 2119 2219 Fragments arranged by size: 2219 2119 956 617 BsaAI yAC'GTr Cuts at: 493 1204 2269 2562 3702 493 Size: 711 1065 293 1140 2702 Fragments arranged by size: 2702 1140 1065 711 293 BsaBI GATnn'nnATC Cuts at: 5905 5905 Size: 5911 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2063 2142 2200 2418 Size: 53 83 186 936 530 79 58 218 Cuts at: 2418 2599 2710 2755 3718 4100 4735 275 Size: 181 111 45 963 382 635 1451 Fragments arranged by size: 1451 963 936 635 530 382 218 186 181 111 83 79 58 53 45 BsaWI w'CCGG_w Cuts at: 4344 4742 5016 5163 5574 4344 Size: 398 274 147 411 4681 Fragments arranged by size: 4681 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5686 Fragments arranged by size: 5686 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2639 1186 Size: 1453 4458 BseYI C'CCAG_C Cuts at: 2137 2383 2760 3139 5114 5610 2137 Size: 246 377 379 1975 496 2438 Fragments arranged by size: 2438 1975 496 379 377 246 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5338 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2088 2214 2322 2429 2457 3361 Size: 275 186 87 126 108 107 28 904 Cuts at: 3361 4122 5150 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 904 761 275 186 126 108 107 87 28 BsiHKAI G_wGCw'C Cuts at: 2869 2981 3972 4057 5128 2869 Size: 112 991 85 1071 3652 Fragments arranged by size: 3652 1071 991 112 85 BsmI GAATG_Cn' Cuts at: 3422 3515 3422 Size: 93 5818 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2022 2375 2561 2869 2939 2981 Size: 399 222 10 353 186 308 70 42 Cuts at: 2981 3230 3972 4057 5128 1391 Size: 249 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 353 308 249 222 186 85 70 42 10 BspEI T'CCGG_A Cuts at: 5574 5574 Size: 5911 BspHI T'CATG_A Cuts at: 3800 5530 3800 Size: 1730 4181 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2163 3266 844 Size: 1319 1103 3489 Fragments arranged by size: 3489 1319 1103 BsrBI CCG'CTC Cuts at: 1394 1823 2208 2715 2781 3798 1394 Size: 429 385 507 66 1017 3507 Fragments arranged by size: 3507 1017 507 429 385 66 BsrDI GCAATG_nn' Cuts at: 65 1248 4406 4580 65 Size: 1183 3158 174 1396 Fragments arranged by size: 3158 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2321 2751 4555 4742 1700 Size: 110 511 430 1804 187 2869 Fragments arranged by size: 2869 1804 511 430 187 110 BsrGI T'GTAC_A Cuts at: 96 2298 3054 96 Size: 2202 756 2953 Fragments arranged by size: 2953 2202 756 BssHII G'CGCG_C Cuts at: 1925 2280 2311 2463 2465 2525 1925 Size: 355 31 152 2 60 5311 Fragments arranged by size: 5311 355 152 60 31 2 BssSI C'ACGA_G Cuts at: 3971 4983 3971 Size: 1012 4899 BstAPI GCAn_nnn'nTGC Cuts at: 794 2565 2612 794 Size: 1771 47 4093 Fragments arranged by size: 4093 1771 47 BstBI TT'CG_AA Cuts at: 4715 4715 Size: 5911 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5911 BstXI CCAn_nnnn'nTGG Cuts at: 2137 2158 2245 2257 2137 Size: 21 87 12 5791 Fragments arranged by size: 5791 87 21 12 BstYI r'GATC_y Cuts at: 1972 3036 3241 3608 3993 4010 5462 5548 5560 Size: 1064 205 367 385 17 1452 86 12 Cuts at: 5560 5906 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1064 385 367 346 205 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2737 2737 Size: 5911 BtgI C'CryG_G Cuts at: 513 1065 2184 2400 2481 2490 2505 3132 513 Size: 552 1119 216 81 9 15 627 3292 Fragments arranged by size: 3292 1119 627 552 216 81 15 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2794 487 Size: 683 56 1568 3604 Fragments arranged by size: 3604 1568 683 56 BtsI GCAGTG_nn' Cuts at: 2301 2606 3427 4221 4241 2301 Size: 305 821 794 20 3971 Fragments arranged by size: 3971 821 794 305 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5911 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2812 2847 529 Size: 35 2248 35 3593 Fragments arranged by size: 3593 2248 35 35 DraI TTT'AAA Cuts at: 3215 3215 Size: 5911 DraIII CAC_nnn'GTG Cuts at: 2565 2881 2565 Size: 316 5595 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4918 809 Size: 507 3602 1802 Fragments arranged by size: 3602 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2085 2211 2235 Size: 54 1425 50 275 18 255 126 24 Cuts at: 2235 2247 2319 2397 2426 2478 2487 2502 2961 Size: 12 72 78 29 52 9 15 459 Cuts at: 2961 2996 3009 3358 4247 5876 8 Size: 35 13 349 889 1629 43 Fragments arranged by size: 1629 1425 889 459 349 275 255 126 78 72 54 52 50 43 35 29 24 18 15 13 12 9 EagI C'GGCC_G Cuts at: 1537 1812 2085 2211 2319 2426 3358 1537 Size: 275 273 126 108 107 932 4090 Fragments arranged by size: 4090 932 275 273 126 108 107 EarI CTCTTCn'nnn_ Cuts at: 1353 2627 3841 1353 Size: 1274 1214 3423 Fragments arranged by size: 3423 1274 1214 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2779 2788 4498 4872 5018 387 Size: 1064 138 1190 9 1710 374 146 1280 Fragments arranged by size: 1710 1280 1190 1064 374 146 138 9 EcoICRI GAG'CTC Cuts at: 2867 2979 2867 Size: 112 5799 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2953 2995 3974 4571 5358 5626 1239 Size: 393 1321 42 979 597 787 268 1524 Fragments arranged by size: 1524 1321 979 787 597 393 268 42 EcoO109I rG'GnC_Cy Cuts at: 2019 2071 2328 2582 2741 2019 Size: 52 257 254 159 5189 Fragments arranged by size: 5189 257 254 159 52 EcoRI G'AATT_C Cuts at: 3220 3598 3220 Size: 378 5533 FspI TGC'GCA Cuts at: 3179 4417 3179 Size: 1238 4673 HaeII r_GCGC'y Cuts at: 1678 2066 2106 2145 2165 2171 2410 2477 2513 Size: 388 40 39 20 6 239 67 36 Cuts at: 2513 2602 2960 3159 5058 1678 Size: 89 358 199 1899 2531 Fragments arranged by size: 2531 1899 388 358 239 199 89 67 40 39 36 20 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4552 4584 4626 4658 1373 Size: 32 135 32 2980 32 42 32 2626 Fragments arranged by size: 2980 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3255 3501 669 Size: 988 281 1317 246 3079 Fragments arranged by size: 3079 1317 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5911 HpaI GTT'AAC Cuts at: 3501 3501 Size: 5911 KasI G'GCGC_C Cuts at: 2062 2141 2598 2062 Size: 79 457 5375 Fragments arranged by size: 5375 457 79 KpnI G_GTAC'C Cuts at: 3236 3236 Size: 5911 MfeI C'AATT_G Cuts at: 3510 3510 Size: 5911 MluI A'CGCG_T Cuts at: 5787 5787 Size: 5911 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2175 5025 5209 1305 Size: 870 2850 184 2007 Fragments arranged by size: 2850 2007 870 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2237 2249 10 Size: 54 1425 343 405 12 3672 Fragments arranged by size: 3672 1425 405 343 54 12 MslI CAynn'nnrTG Cuts at: 518 1166 2399 2915 3869 4228 4387 518 Size: 648 1233 516 954 359 159 2042 Fragments arranged by size: 2042 1233 954 648 516 359 159 MspA1I CmG'CkG Cuts at: 1606 2186 2377 2431 2483 2492 2521 2679 2703 Size: 580 191 54 52 9 29 158 24 Cuts at: 2703 2850 2995 3134 3195 3327 4004 5152 5397 Size: 147 145 139 61 132 677 1148 245 Cuts at: 5397 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 580 245 191 158 147 145 139 132 61 54 52 29 24 9 NaeI GCC'GGC Cuts at: 2323 2753 2323 Size: 430 5481 NarI GG'CG_CC Cuts at: 2063 2142 2599 2063 Size: 79 457 5375 Fragments arranged by size: 5375 457 79 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5359 NdeI CA'TA_TG Cuts at: 387 387 Size: 5911 NgoMIV G'CCGG_C Cuts at: 2321 2751 2321 Size: 430 5481 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5911 NotI GC'GGCC_GC Cuts at: 2426 3358 2426 Size: 932 4979 NspI r_CATG'y Cuts at: 1132 1229 2058 3269 4814 1132 Size: 97 829 1211 1545 2229 Fragments arranged by size: 2229 1545 1211 829 97 PasI CC'CwG_GG Cuts at: 1348 2067 1348 Size: 719 5192 PciI A'CATG_T Cuts at: 4810 4810 Size: 5911 PflMI CCAn_nnn'nTGG Cuts at: 1108 2341 2881 3040 1108 Size: 1233 540 159 3979 Fragments arranged by size: 3979 1233 540 159 PfoI T'CCnGG_A Cuts at: 3026 3039 3026 Size: 13 5898 PmeI GTTT'AAAC Cuts at: 3215 3215 Size: 5911 PmlI CAC'GTG Cuts at: 2269 2562 2269 Size: 293 5618 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3980 4012 5493 5525 3980 Size: 32 1481 32 4366 Fragments arranged by size: 4366 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2071 2582 2071 Size: 511 5400 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5911 PsiI TTA'TAA Cuts at: 3481 3481 Size: 5911 PspOMI G'GGCC_C Cuts at: 2018 2018 Size: 5911 PstI C_TGCA'G Cuts at: 830 2550 2994 3263 830 Size: 1720 444 269 3478 Fragments arranged by size: 3478 1720 444 269 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5911 SacI G_AGCT'C Cuts at: 2869 2981 2869 Size: 112 5799 SacII CC_GC'GG Cuts at: 2187 2484 2493 3135 2187 Size: 297 9 642 4963 Fragments arranged by size: 4963 642 297 9 SalI G'TCGA_C Cuts at: 1936 3253 1936 Size: 1317 4594 SanDI GG'GwC_CC Cuts at: 2071 2582 2071 Size: 511 5400 SbfI CC_TGCA'GG Cuts at: 3263 3263 Size: 5911 ScaI AGT'ACT Cuts at: 4159 5871 4159 Size: 1712 4199 SfcI C'TryA_G Cuts at: 826 1045 2546 2990 3259 4394 5075 5266 826 Size: 219 1501 444 269 1135 681 191 1471 Fragments arranged by size: 1501 1471 1135 681 444 269 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2327 2486 2495 2327 Size: 159 9 5743 Fragments arranged by size: 5743 159 9 SfoI GGC'GCC Cuts at: 2064 2143 2600 2064 Size: 79 457 5375 Fragments arranged by size: 5375 457 79 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5911 SmaI CCC'GGG Cuts at: 1872 2277 2556 2589 3238 1872 Size: 405 279 33 649 4545 Fragments arranged by size: 4545 649 405 279 33 SmlI C'TyrA_G Cuts at: 820 1017 1943 4015 4916 5178 820 Size: 197 926 2072 901 262 1553 Fragments arranged by size: 2072 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3702 493 Size: 3209 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5911 SphI G_CATG'C Cuts at: 1132 3269 1132 Size: 2137 3774 SspI AAT'ATT Cuts at: 5 52 3835 5 Size: 47 3783 2081 Fragments arranged by size: 3783 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5911 StyI C'CwwG_G Cuts at: 513 1065 2499 3047 3120 3350 513 Size: 552 1434 548 73 230 3074 Fragments arranged by size: 3074 1434 552 548 230 73 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2443 3040 3938 3955 4108 4293 4738 Size: 1255 863 597 898 17 153 185 445 Cuts at: 4738 325 Size: 1498 Fragments arranged by size: 1498 1255 898 863 597 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2298 3054 4157 Size: 274 80 33 51 1443 321 756 1103 Cuts at: 4157 5869 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1103 756 321 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5871 5903 5871 Size: 32 5879 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5911 XbaI T'CTAG_A Cuts at: 3247 3247 Size: 5911 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2635 2635 Size: 5911 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5911 XmaI C'CCGG_G Cuts at: 1870 2275 2554 2587 3236 1870 Size: 405 279 33 649 4545 Fragments arranged by size: 4545 649 405 279 33 XmnI GAAnn'nnTTC Cuts at: 4040 4040 Size: 5911 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3719 276 Size: 53 83 186 936 2185 2468 Fragments arranged by size: 2468 2185 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI PvuII RsrII SapI SexAI SrfI SwaI