(Circular) (Six-base) MAPSORT of: pFN21AE3025.seq Check: 7767 from: 1 to: 5131 pFN21AE3025 5131 bp With 182 enzymes: SgfI * March 28, 2012 12:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2150 2150 Size: 5131 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2941 278 Size: 53 83 186 936 1405 2468 Fragments arranged by size: 2468 1405 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2474 1937 Size: 537 4594 Acc65I G'GTAC_C Cuts at: 2452 2452 Size: 5131 AclI AA'CG_TT Cuts at: 1563 3258 3631 1563 Size: 1695 373 3063 Fragments arranged by size: 3063 1695 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3194 4578 4846 1632 Size: 1562 1384 268 1917 Fragments arranged by size: 1917 1562 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4934 AflIII A'CryG_T Cuts at: 1203 4030 5007 1203 Size: 2827 977 1327 Fragments arranged by size: 2827 1327 977 AgeI A'CCGG_T Cuts at: 3962 3962 Size: 5131 AhdI GACnn_n'nnGTC Cuts at: 3860 3860 Size: 5131 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5131 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5131 AlwNI CAG_nnn'CTG Cuts at: 2219 2385 2388 2874 4446 2219 Size: 166 3 486 1572 2904 Fragments arranged by size: 2904 1572 486 166 3 ApaLI G'TGCA_C Cuts at: 3188 4344 3188 Size: 1156 3975 ApoI r'AATT_y Cuts at: 1493 2117 2440 2666 2818 4927 1493 Size: 624 323 226 152 2109 1697 Fragments arranged by size: 2109 1697 624 323 226 152 AseI AT'TA_AT Cuts at: 160 1032 3685 160 Size: 872 2653 1606 Fragments arranged by size: 2653 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5131 AvaI C'yCGr_G Cuts at: 1870 1943 2079 2456 1870 Size: 73 136 377 4545 Fragments arranged by size: 4545 377 136 73 BamHI G'GATC_C Cuts at: 2461 2828 2461 Size: 367 4764 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2452 3907 618 Size: 325 217 627 665 1455 1842 Fragments arranged by size: 1842 1455 665 627 325 217 BanII G_rGCy'C Cuts at: 1391 2153 2450 1391 Size: 762 297 4072 Fragments arranged by size: 4072 762 297 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2232 928 Size: 971 333 3827 Fragments arranged by size: 3827 971 333 BbvCI CC'TCA_GC Cuts at: 2073 2073 Size: 5131 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3322 3356 1903 Size: 34 1385 34 3678 Fragments arranged by size: 3678 1385 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3023 4233 3023 Size: 1210 3921 BclI T'GATC_A Cuts at: 1548 1548 Size: 5131 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2186 3742 136 Size: 107 122 71 631 605 514 1556 1525 Fragments arranged by size: 1556 1525 631 605 514 122 107 71 BglII A'GATC_T Cuts at: 5126 5126 Size: 5131 BlpI GC'TnA_GC Cuts at: 2548 2548 Size: 5131 Bme1580I G_kGCm'C Cuts at: 1790 3192 4348 1790 Size: 1402 1156 2573 Fragments arranged by size: 2573 1402 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5131 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2862 3820 442 Size: 416 53 83 399 24 1445 958 1753 Fragments arranged by size: 1753 1445 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5131 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3791 1239 Size: 2552 2579 Bpu10I CC'TnA_GC Cuts at: 2073 2073 Size: 5131 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3256 4121 4419 3256 Size: 865 298 3968 Fragments arranged by size: 3968 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3794 882 Size: 617 2295 2219 Fragments arranged by size: 2295 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2922 493 Size: 711 1718 2702 Fragments arranged by size: 2702 1718 711 BsaBI GATnn'nnATC Cuts at: 5125 5125 Size: 5131 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2938 3320 3955 275 Size: 53 83 186 936 1405 382 635 1451 Fragments arranged by size: 1451 1405 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3564 3962 4236 4383 4794 3564 Size: 398 274 147 411 3901 Fragments arranged by size: 3901 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4906 Fragments arranged by size: 4906 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5131 BseYI C'CCAG_C Cuts at: 2318 4334 4830 2318 Size: 2016 496 2619 Fragments arranged by size: 2619 2016 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4558 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2113 2581 3342 4370 1540 Size: 275 186 112 468 761 1028 2301 Fragments arranged by size: 2301 1028 761 468 275 186 112 BsiHKAI G_wGCw'C Cuts at: 3192 3277 4348 3192 Size: 85 1071 3975 Fragments arranged by size: 3975 1071 85 BsmI GAATG_Cn' Cuts at: 2642 2735 2642 Size: 93 5038 BsmBI CGTCTCn'nnnn_ Cuts at: 2155 2155 Size: 5131 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2153 2450 3192 3277 4348 1391 Size: 399 363 297 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 363 297 85 BspEI T'CCGG_A Cuts at: 4794 4794 Size: 5131 BspHI T'CATG_A Cuts at: 3020 4750 3020 Size: 1730 3401 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2150 2486 844 Size: 1306 336 3489 Fragments arranged by size: 3489 1306 336 BsrBI CCG'CTC Cuts at: 1394 1823 3018 1394 Size: 429 1195 3507 Fragments arranged by size: 3507 1195 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3626 3800 65 Size: 1183 2378 174 1396 Fragments arranged by size: 2378 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3775 3962 1700 Size: 110 1965 187 2869 Fragments arranged by size: 2869 1965 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5131 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5131 BssSI C'ACGA_G Cuts at: 2183 3191 4203 2183 Size: 1008 1012 3111 Fragments arranged by size: 3111 1012 1008 BstAPI GCAn_nnn'nTGC Cuts at: 794 2388 794 Size: 1594 3537 BstBI TT'CG_AA Cuts at: 3935 3935 Size: 5131 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5131 BstYI r'GATC_y Cuts at: 1972 2461 2828 3213 3230 4682 4768 4780 5126 Size: 489 367 385 17 1452 86 12 346 Cuts at: 5126 1972 Size: 1977 Fragments arranged by size: 1977 1452 489 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2256 513 Size: 552 939 252 3388 Fragments arranged by size: 3388 939 552 252 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4392 Fragments arranged by size: 4392 683 56 BtsI GCAGTG_nn' Cuts at: 2647 3441 3461 2647 Size: 794 20 4317 Fragments arranged by size: 4317 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5131 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5096 DraI TTT'AAA Cuts at: 2435 2435 Size: 5131 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4138 809 Size: 507 2822 1802 Fragments arranged by size: 2822 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2063 2110 Size: 54 1425 50 275 18 177 56 47 Cuts at: 2110 2578 3467 5096 8 Size: 468 889 1629 43 Fragments arranged by size: 1629 1425 889 468 275 177 56 54 50 47 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2110 2578 1537 Size: 275 298 468 4090 Fragments arranged by size: 4090 468 298 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3061 1353 Size: 1708 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3718 4092 4238 387 Size: 1064 138 2129 374 146 1280 Fragments arranged by size: 2129 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3194 3791 4578 4846 1239 Size: 393 1562 597 787 268 1524 Fragments arranged by size: 1562 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2305 2305 Size: 5131 EcoRI G'AATT_C Cuts at: 2117 2440 2818 2117 Size: 323 378 4430 Fragments arranged by size: 4430 378 323 FspI TGC'GCA Cuts at: 3637 3637 Size: 5131 HaeII r_GCGC'y Cuts at: 1678 2090 4278 1678 Size: 412 2188 2531 Fragments arranged by size: 2531 2188 412 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2106 2118 2138 2150 2157 Size: 32 135 32 534 12 20 12 7 Cuts at: 2157 2189 3772 3804 3846 3878 1373 Size: 32 1583 32 42 32 2626 Fragments arranged by size: 2626 1583 534 135 42 32 32 32 32 32 20 12 12 7 HincII GTy'rAC Cuts at: 669 1657 1938 2475 2721 669 Size: 988 281 537 246 3079 Fragments arranged by size: 3079 988 537 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5131 HpaI GTT'AAC Cuts at: 2721 2721 Size: 5131 KpnI G_GTAC'C Cuts at: 2456 2456 Size: 5131 MfeI C'AATT_G Cuts at: 2730 2730 Size: 5131 MluI A'CGCG_T Cuts at: 5007 5007 Size: 5131 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4245 4429 1305 Size: 2940 184 2007 Fragments arranged by size: 2940 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 3132 Fragments arranged by size: 3132 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3089 3448 3607 518 Size: 648 1923 359 159 2042 Fragments arranged by size: 2042 1923 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2322 2385 2547 3224 4372 4617 1606 Size: 716 63 162 677 1148 245 2120 Fragments arranged by size: 2120 1148 716 677 245 162 63 NcoI C'CATG_G Cuts at: 513 1065 2004 2256 513 Size: 552 939 252 3388 Fragments arranged by size: 3388 939 552 252 NdeI CA'TA_TG Cuts at: 387 387 Size: 5131 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5131 NotI GC'GGCC_GC Cuts at: 2578 2578 Size: 5131 NspI r_CATG'y Cuts at: 1132 1229 2020 2489 4034 1132 Size: 97 791 469 1545 2229 Fragments arranged by size: 2229 1545 791 469 97 PasI CC'CwG_GG Cuts at: 1348 2336 1348 Size: 988 4143 PciI A'CATG_T Cuts at: 4030 4030 Size: 5131 PflMI CCAn_nnn'nTGG Cuts at: 1108 2271 1108 Size: 1163 3968 PfoI T'CCnGG_A Cuts at: 2205 2205 Size: 5131 PmeI GTTT'AAAC Cuts at: 2435 2435 Size: 5131 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3200 3232 4713 4745 3200 Size: 32 1481 32 3586 Fragments arranged by size: 3586 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5131 PsiI TTA'TAA Cuts at: 2701 2701 Size: 5131 PstI C_TGCA'G Cuts at: 830 2483 830 Size: 1653 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5131 PvuII CAG'CTG Cuts at: 2322 2385 2322 Size: 63 5068 SalI G'TCGA_C Cuts at: 1936 2473 1936 Size: 537 4594 SbfI CC_TGCA'GG Cuts at: 2483 2483 Size: 5131 ScaI AGT'ACT Cuts at: 3379 5091 3379 Size: 1712 3419 SfcI C'TryA_G Cuts at: 826 1045 2479 3614 4295 4486 826 Size: 219 1434 1135 681 191 1471 Fragments arranged by size: 1471 1434 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5131 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5131 SmaI CCC'GGG Cuts at: 1872 2081 2458 1872 Size: 209 377 4545 Fragments arranged by size: 4545 377 209 SmlI C'TyrA_G Cuts at: 820 1017 1943 3235 4136 4398 820 Size: 197 926 1292 901 262 1553 Fragments arranged by size: 1553 1292 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2922 493 Size: 2429 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5131 SphI G_CATG'C Cuts at: 1132 2489 1132 Size: 1357 3774 SrfI GCCC'GGGC Cuts at: 2081 2081 Size: 5131 SspI AAT'ATT Cuts at: 5 52 3055 5 Size: 47 3003 2081 Fragments arranged by size: 3003 2081 47 StuI AGG'CCT Cuts at: 1517 2300 1517 Size: 783 4348 StyI C'CwwG_G Cuts at: 513 1065 2004 2256 2570 513 Size: 552 939 252 314 3074 Fragments arranged by size: 3074 939 552 314 252 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2032 3158 3175 3328 3513 3958 325 Size: 1255 452 1126 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1126 452 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3377 5089 96 Size: 274 80 33 51 1443 1400 1712 138 Fragments arranged by size: 1712 1443 1400 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5091 5123 5091 Size: 32 5099 Tth111I GACn'n_nGTC Cuts at: 1316 2287 1316 Size: 971 4160 XbaI T'CTAG_A Cuts at: 2467 2467 Size: 5131 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2272 2272 Size: 5131 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5131 XmaI C'CCGG_G Cuts at: 1870 2079 2456 1870 Size: 209 377 4545 Fragments arranged by size: 4545 377 209 XmnI GAAnn'nnTTC Cuts at: 3260 3260 Size: 5131 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2939 276 Size: 53 83 186 936 1405 2468 Fragments arranged by size: 2468 1405 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SwaI