################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BfrI BglII BlnI BsaAI BsaBI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstUI BstZ17I ClaI CpoI DraI DraIII EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FseI FspI HpaI KasI KpnI KspI MaeII MamI MfeI MluI MroI MunI MvnI NarI NcoI NdeI NheI NotI NruI PacI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Van91I XbaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcyI 1636 GACGCC G[GA]^CG[CT]C AfaI 833 GTAC GT^AC AhdI 628 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 226 GGATC GGATC(4/5) Asp700I 288 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 628 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1026 GAGCAC G[AT]GC[AT]^C BclI 1769 TGATCA T^GATCA BlpI 1492 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1492 GCTCAGC GC^T[ACGT]AGC BsaHI 1636 GACGCC G[GA]^CG[CT]C BsiHKAI 1026 GAGCAC G[AT]GC[AT]^C BsmI 1502 GCATTC GAATGC(1/-1) BsrBI 1272 GAGCGG CCGCTC(-3/-3) BstBI 1648 TTCGAA TT^CGAA BstYI 226 GGATCT [GA]^GATC[CT] CelII 1492 GCTCAGC GC^T[ACGT]AGC DrdI 594 GACGGGGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 628 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT22I 1310 ATGCAT ATGCA^T FbaI 1769 TGATCA T^GATCA HgaI 1636 GACGC GACGC(5/10) Hin1I 1636 GACGCC G[GA]^CG[CT]C HincII 248 GTCAAC GT[CT]^[GA]AC HindII 248 GTCAAC GT[CT]^[GA]AC HindIII 1796 AAGCTT A^AGCTT MflI 226 GGATCT [GA]^GATC[CT] MseI 1811 TTAA T^TAA MslI 1720 CATGCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NsiI 1310 ATGCAT ATGCA^T NspI 1719 ACATGC [GA]CATG^[CT] NspV 1648 TTCGAA TT^CGAA PshAI 1321 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1556 CTGCAG CTGCA^G PvuII 433 CAGCTG CAG^CTG RsaI 833 GTAC GT^AC SfuI 1648 TTCGAA TT^CGAA Tru9I 1811 TTAA T^TAA Tsp509I 146 AATT ^AATT XcmI 1514 CCACCTCAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 226 GGATCT [GA]^GATC[CT] XmnI 288 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AspI 38 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 820 GACGGTGTC BbsI 330 GAAGAC GAAGAC(2/6) 1821 GAAGAC BpuAI 330 GAAGAC GAAGAC(2/6) 1821 GAAGAC BsaWI 1303 TCCGGT [AT]^CCGG[AT] 1817 ACCGGA BsgI 1158 CTGCAC GTGCAG(16/14) 538 GTGCAG BsmBI 198 CGTCTC CGTCTC(1/5) 818 GAGACG BspMI 1101 ACCTGC ACCTGC(4/8) 1544 ACCTGC BsrFI 236 GCCGGC [GA]^CCGG[CT] 1763 GCCGGC BstAPI 499 GCAGACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 540 GCAGGCGGTGC CfoI 413 GCGC GCG^C 1806 GCGC Cfr10I 236 GCCGGC [GA]^CCGG[CT] 1763 GCCGGC DpnII 227 GATC ^GATC 1770 GATC DsaI 369 CCGTGG C^C[GA][CT]GG 473 CCACGG EarI 349 GAAGAG CTCTTC(1/4) 1048 GAAGAG FauI 509 CCCGC CCCGC(4/6) 1428 CCCGC HaeII 412 AGCGCC [GA]GCGC^[CT] 1805 AGCGCC HhaI 413 GCGC GCG^C 1806 GCGC HinP1I 413 GCGC G^CGC 1806 GCGC Ksp632I 349 GAAGAG CTCTTC(1/4) 1048 GAAGAG MaeIII 337 GTGAC ^GT[ACGT]AC 686 GTGAC MboI 227 GATC ^GATC 1770 GATC MspA1I 433 CAGCTG C[AC]G^C[GT]G 1124 CCGCTG NaeI 236 GCCGGC GCC^GGC 1763 GCCGGC NdeII 227 GATC ^GATC 1770 GATC NgoMIV 236 GCCGGC G^CCGGC 1763 GCCGGC PaeR7I 1408 CTCGAG C^TCGAG 1883 CTCGAG PflFI 38 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 820 GACGGTGTC Sau3AI 227 GATC ^GATC 1770 GATC SfcI 1244 CTACAG C^T[GA][CT]AG 1556 CTGCAG SmaI 901 CCCGGG CCC^GGG 1486 CCCGGG Tsp45I 337 GTGAC ^GT[GC]AC 686 GTGAC Tth111I 38 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 820 GACGGTGTC XhoI 1408 CTCGAG C^TCGAG 1883 CTCGAG XmaCI 901 CCCGGG C^CCGGG 1486 CCCGGG XmaI 901 CCCGGG C^CCGGG 1486 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AluI AlwNI AocI ApaI AvaI AvaII BalI BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Bsu36I Cac8I Cfr13I DdeI DraII EaeI Eco81I EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MboII MluNI MnlI MscI MspI MvaI MwoI NciI NlaIII NlaIV PleI PpuMI Sau96I ScrFI SfaNI SmlI StyI TaqI TfiI TseI TspRI TthHB8I