Restriction map for
pFN21AE9692
(Circular) (Six-base) MAPSORT of: pFN21AE9692.seq Check: 2779 from: 1 to: 6616
pFN21AE9692 6616 bp
With 182 enzymes: SgfI *
December 8, 2014 13:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3115 3558 3115
Size: 443 6173
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4426 278
Size: 53 83 186 936 2890 2468
Fragments arranged by size:
2890 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3959 1937
Size: 2022 4594
Acc65I G'GTAC_C
Cuts at: 3937 3937
Size: 6616
AclI AA'CG_TT
Cuts at: 1563 4743 5116 1563
Size: 3180 373 3063
Fragments arranged by size:
3180 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2751 4679 6063 6331 1632
Size: 1119 1928 1384 268 1917
Fragments arranged by size:
1928 1917 1384 1119 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6419
AflIII A'CryG_T
Cuts at: 1203 5515 6492 1203
Size: 4312 977 1327
Fragments arranged by size:
4312 1327 977
AgeI A'CCGG_T
Cuts at: 5447 5447
Size: 6616
AhdI GACnn_n'nnGTC
Cuts at: 2638 5345 2638
Size: 2707 3909
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6616
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3111 1184
Size: 1927 4689
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3591 3623 3591
Size: 32 6584
AlwNI CAG_nnn'CTG
Cuts at: 2792 2867 3365 4359 5931 2792
Size: 75 498 994 1572 3477
Fragments arranged by size:
3477 1572 994 498 75
ApaI G_GGCC'C
Cuts at: 2776 2811 2885 3081 3248 3701 3716 2776
Size: 35 74 196 167 453 15 5676
Fragments arranged by size:
5676 453 196 167 74 35 15
ApaLI G'TGCA_C
Cuts at: 4673 5829 4673
Size: 1156 5460
ApoI r'AATT_y
Cuts at: 1493 3925 4151 4303 6412 1493
Size: 2432 226 152 2109 1697
Fragments arranged by size:
2432 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5170 160
Size: 872 4138 1606
Fragments arranged by size:
4138 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6616
AvaI C'yCGr_G
Cuts at: 1870 1943 2906 3005 3413 3491 3888 3941 1870
Size: 73 963 99 408 78 397 53 4545
Fragments arranged by size:
4545 963 408 397 99 78 73 53
BamHI G'GATC_C
Cuts at: 3946 4313 3946
Size: 367 6249
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2551 2895 3268 3572 3782
Size: 325 217 627 764 344 373 304 210
Cuts at: 3782 3937 5392 618
Size: 155 1455 1842
Fragments arranged by size:
1842 1455 764 627 373 344 325 304
217 210 155
BanII G_rGCy'C
Cuts at: 1391 2540 2758 2776 2811 2885 2927 3064 3081
Size: 1149 218 18 35 74 42 137 17
Cuts at: 3081 3248 3492 3499 3701 3716 3935 1391
Size: 167 244 7 202 15 219 4072
Fragments arranged by size:
4072 1149 244 219 218 202 167 137
74 42 35 18 17 15 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2342 3833 928
Size: 971 443 1491 3711
Fragments arranged by size:
3711 1491 971 443
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4807 4841 1903
Size: 34 2870 34 3678
Fragments arranged by size:
3678 2870 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4508 5718 4508
Size: 1210 5406
BclI T'GATC_A
Cuts at: 1548 3774 1548
Size: 2226 4390
BfrBI ATG'CAT
Cuts at: 3317 3317
Size: 6616
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2806 3085 3260
Size: 107 122 71 631 605 1134 279 175
Cuts at: 3260 3769 5227 136
Size: 509 1458 1525
Fragments arranged by size:
1525 1458 1134 631 605 509 279 175
122 107 71
BglII A'GATC_T
Cuts at: 6611 6611
Size: 6616
BlpI GC'TnA_GC
Cuts at: 3498 4033 3498
Size: 535 6081
Bme1580I G_kGCm'C
Cuts at: 1790 2515 2556 2776 2811 2885 3081 3248 3575
Size: 725 41 220 35 74 196 167 327
Cuts at: 3575 3701 3716 3785 4677 5833 1790
Size: 126 15 69 892 1156 2573
Fragments arranged by size:
2573 1156 892 725 327 220 196 167
126 74 69 41 35 15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6616
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2311 2888 3186
Size: 416 53 83 399 24 894 577 298
Cuts at: 3186 4347 5305 442
Size: 1161 958 1753
Fragments arranged by size:
1753 1161 958 894 577 416 399 298
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6616
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2060 2060
Size: 6616
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2191 2453 2571 2823 2856 3086 3321 3467
Size: 952 262 118 252 33 230 235 146
Cuts at: 3467 3925 5276 1239
Size: 458 1351 2579
Fragments arranged by size:
2579 1351 952 458 262 252 235 230
146 118 33
Bpu10I CC'TnA_GC
Cuts at: 2520 3029 3666 2520
Size: 509 637 5470
Fragments arranged by size:
5470 637 509
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3354 4741 5606 5904 3354
Size: 1387 865 298 4066
Fragments arranged by size:
4066 1387 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2444 3402 3537 3729 5279 882
Size: 617 945 958 135 192 1550 2219
Fragments arranged by size:
2219 1550 958 945 617 192 135
BsaAI yAC'GTr
Cuts at: 493 1204 4407 493
Size: 711 3203 2702
Fragments arranged by size:
3203 2702 711
BsaBI GATnn'nnATC
Cuts at: 6610 6610
Size: 6616
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3642 4423 4805 5440
Size: 53 83 186 936 2109 781 382 635
Cuts at: 5440 275
Size: 1451
Fragments arranged by size:
2109 1451 936 781 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3308 3822 5049 5447 5721 5868 6279 3308
Size: 514 1227 398 274 147 411 3645
Fragments arranged by size:
3645 1227 514 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2218 2248 2814 2844 3726
Size: 30 165 30 815 30 566 30 882
Cuts at: 3726 3756 1178
Size: 30 4038
Fragments arranged by size:
4038 882 815 566 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2150 2237 2766 2831 2989 1186
Size: 964 87 529 65 158 4813
Fragments arranged by size:
4813 964 529 158 87 65
BseYI C'CCAG_C
Cuts at: 2485 2842 2937 2948 3074 3716 3759 3835 5819
Size: 357 95 11 126 642 43 76 1984
Cuts at: 5819 6315 2485
Size: 496 2786
Fragments arranged by size:
2786 1984 642 496 357 126 95 76
43 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2564 3148 1163
Size: 573 828 584 4631
Fragments arranged by size:
4631 828 584 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4066 4827 5855 1540
Size: 275 186 2065 761 1028 2301
Fragments arranged by size:
2301 2065 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3035 4677 4762 5833 3035
Size: 1642 85 1071 3818
Fragments arranged by size:
3818 1642 1071 85
BsmI GAATG_Cn'
Cuts at: 3507 4127 4220 3507
Size: 620 93 5903
Fragments arranged by size:
5903 620 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2209 2817 2209
Size: 608 6008
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2515 2540 2556 2758 2776 2811 2885
Size: 399 725 25 16 202 18 35 74
Cuts at: 2885 2927 3035 3064 3081 3248 3492 3499 3575
Size: 42 108 29 17 167 244 7 76
Cuts at: 3575 3701 3716 3785 3935 4677 4762 5833 1391
Size: 126 15 69 150 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 725 399 244 202 167
150 126 108 85 76 74 69 42
35 29 25 18 17 16 15 7
BspEI T'CCGG_A
Cuts at: 6279 6279
Size: 6616
BspHI T'CATG_A
Cuts at: 4505 6235 4505
Size: 1730 4886
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3115 3558 3971 844
Size: 2271 443 413 3489
Fragments arranged by size:
3489 2271 443 413
BsrBI CCG'CTC
Cuts at: 1394 1823 3279 4503 1394
Size: 429 1456 1224 3507
Fragments arranged by size:
3507 1456 1224 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5111 5285 65
Size: 1183 3863 174 1396
Fragments arranged by size:
3863 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2241 3768 5260 5447 1700
Size: 110 431 1527 1492 187 2869
Fragments arranged by size:
2869 1527 1492 431 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6616
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6616
BssSI C'ACGA_G
Cuts at: 4676 5688 4676
Size: 1012 5604
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2510 2551 794
Size: 1716 41 4859
Fragments arranged by size:
4859 1716 41
BstBI TT'CG_AA
Cuts at: 3654 5420 3654
Size: 1766 4850
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6616
BstXI CCAn_nnnn'nTGG
Cuts at: 2485 2956 3049 2485
Size: 471 93 6052
Fragments arranged by size:
6052 471 93
BstYI r'GATC_y
Cuts at: 1972 2231 3946 4313 4698 4715 6167 6253 6265
Size: 259 1715 367 385 17 1452 86 12
Cuts at: 6265 6611 1972
Size: 346 1977
Fragments arranged by size:
1977 1715 1452 385 367 346 259 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2032 2133 2860 2032
Size: 101 727 5788
Fragments arranged by size:
5788 727 101
BtgI C'CryG_G
Cuts at: 513 1065 2374 2478 513
Size: 552 1309 104 4651
Fragments arranged by size:
4651 1309 552 104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3004 3246 487
Size: 683 56 1778 242 3857
Fragments arranged by size:
3857 1778 683 242 56
BtsI GCAGTG_nn'
Cuts at: 2566 3753 4132 4926 4946 2566
Size: 1187 379 794 20 4236
Fragments arranged by size:
4236 1187 794 379 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6616
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6581
DraI TTT'AAA
Cuts at: 3920 3920
Size: 6616
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2605 5623 809
Size: 507 1289 3018 1802
Fragments arranged by size:
3018 1802 1289 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2698 3391 3707
Size: 54 1425 50 275 18 868 693 316
Cuts at: 3707 3750 4063 4952 6581 8
Size: 43 313 889 1629 43
Fragments arranged by size:
1629 1425 889 868 693 316 313 275
54 50 43 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4063 1537
Size: 275 2251 4090
Fragments arranged by size:
4090 2251 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2349 3048 4546 1353
Size: 996 699 1498 3423
Fragments arranged by size:
3423 1498 996 699
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2599 5203 5577 5723 387
Size: 1064 138 1010 2604 374 146 1280
Fragments arranged by size:
2604 1280 1064 1010 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2191 2453 2571 2751 2823 2856 3086
Size: 393 559 262 118 180 72 33 230
Cuts at: 3086 3321 3467 3925 4679 5276 6063 6331 1239
Size: 235 146 458 754 597 787 268 1524
Fragments arranged by size:
1524 787 754 597 559 458 393 268
262 235 230 180 146 118 72 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2471 2617 2859 2871 3115 2471
Size: 146 242 12 244 5972
Fragments arranged by size:
5972 244 242 146 12
EcoO109I rG'GnC_Cy
Cuts at: 2095 2156 2385 2461 2491 2772 2807 2808 2864
Size: 61 229 76 30 281 35 1 56
Cuts at: 2864 2882 3150 3245 3362 3698 3713 3793 3872
Size: 18 268 95 117 336 15 80 79
Cuts at: 3872 3895 2095
Size: 23 4816
Fragments arranged by size:
4816 336 281 268 229 117 95 80
79 76 61 56 35 30 23 18
15 1
EcoRI G'AATT_C
Cuts at: 3925 4303 3925
Size: 378 6238
FspI TGC'GCA
Cuts at: 5122 5122
Size: 6616
HaeII r_GCGC'y
Cuts at: 1678 2421 3814 5763 1678
Size: 743 1393 1949 2531
Fragments arranged by size:
2531 1949 1393 743
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2624 2656 2814 2846 3506
Size: 32 135 32 1052 32 158 32 660
Cuts at: 3506 3538 5257 5289 5331 5363 1373
Size: 32 1719 32 42 32 2626
Fragments arranged by size:
2626 1719 1052 660 158 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2255 3960 4206 669
Size: 988 281 317 1705 246 3079
Fragments arranged by size:
3079 1705 988 317 281 246
HindIII A'AGCT_T
Cuts at: 748 3801 748
Size: 3053 3563
HpaI GTT'AAC
Cuts at: 4206 4206
Size: 6616
KpnI G_GTAC'C
Cuts at: 3941 3941
Size: 6616
MfeI C'AATT_G
Cuts at: 4215 4215
Size: 6616
MluI A'CGCG_T
Cuts at: 6492 6492
Size: 6616
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5730 5914 1305
Size: 4425 184 2007
Fragments arranged by size:
4425 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2700 3393 3752 10
Size: 54 1425 343 868 693 359 2874
Fragments arranged by size:
2874 1425 868 693 359 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3729 4574 4933 5092 518
Size: 648 2563 845 359 159 2042
Fragments arranged by size:
2563 2042 845 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2440 3131 4032 4709 5857 6102 1606
Size: 834 691 901 677 1148 245 2120
Fragments arranged by size:
2120 1148 901 834 691 677 245
NaeI GCC'GGC
Cuts at: 2243 3770 2243
Size: 1527 5089
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6064
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6616
NgoMIV G'CCGG_C
Cuts at: 2241 3768 2241
Size: 1527 5089
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6616
NotI GC'GGCC_GC
Cuts at: 4063 4063
Size: 6616
NsiI A_TGCA'T
Cuts at: 3319 3319
Size: 6616
NspI r_CATG'y
Cuts at: 1132 1229 3728 3974 5519 1132
Size: 97 2499 246 1545 2229
Fragments arranged by size:
2499 2229 1545 246 97
PasI CC'CwG_GG
Cuts at: 1348 2455 2920 1348
Size: 1107 465 5044
Fragments arranged by size:
5044 1107 465
PciI A'CATG_T
Cuts at: 5515 5515
Size: 6616
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6616
PfoI T'CCnGG_A
Cuts at: 2330 2428 2469 2872 3483 2330
Size: 98 41 403 611 5463
Fragments arranged by size:
5463 611 403 98 41
PmeI GTTT'AAAC
Cuts at: 3920 3920
Size: 6616
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3396 3428 4685 4717 6198 6230 3396
Size: 32 1257 32 1481 32 3782
Fragments arranged by size:
3782 1481 1257 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2095 2156 2461 2491 3362 3793 3895 2095
Size: 61 305 30 871 431 102 4816
Fragments arranged by size:
4816 871 431 305 102 61 30
PshAI GACnn'nnGTC
Cuts at: 1613 3330 1613
Size: 1717 4899
PsiI TTA'TAA
Cuts at: 4186 4186
Size: 6616
PspOMI G'GGCC_C
Cuts at: 2772 2807 2881 3077 3244 3697 3712 2772
Size: 35 74 196 167 453 15 5676
Fragments arranged by size:
5676 453 196 167 74 35 15
PspXI vC'TCGA_Gb
Cuts at: 3413 3888 3413
Size: 475 6141
PstI C_TGCA'G
Cuts at: 830 3565 3968 830
Size: 2735 403 3478
Fragments arranged by size:
3478 2735 403
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6616
PvuII CAG'CTG
Cuts at: 2440 2440
Size: 6616
SalI G'TCGA_C
Cuts at: 1936 3958 1936
Size: 2022 4594
SanDI GG'GwC_CC
Cuts at: 2095 2461 2491 3895 2095
Size: 366 30 1404 4816
Fragments arranged by size:
4816 1404 366 30
SbfI CC_TGCA'GG
Cuts at: 3968 3968
Size: 6616
ScaI AGT'ACT
Cuts at: 4864 6576 4864
Size: 1712 4904
SfcI C'TryA_G
Cuts at: 826 1045 3249 3561 3964 5099 5780 5971 826
Size: 219 2204 312 403 1135 681 191 1471
Fragments arranged by size:
2204 1471 1135 681 403 312 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6616
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6616
SmaI CCC'GGG
Cuts at: 1872 2908 3493 3943 1872
Size: 1036 585 450 4545
Fragments arranged by size:
4545 1036 585 450
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3369 3413 3888 4720 5621 5883
Size: 197 926 1426 44 475 832 901 262
Cuts at: 5883 820
Size: 1553
Fragments arranged by size:
1553 1426 926 901 832 475 262 197
44
SnaBI TAC'GTA
Cuts at: 493 4407 493
Size: 3914 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6616
SphI G_CATG'C
Cuts at: 1132 3974 1132
Size: 2842 3774
SspI AAT'ATT
Cuts at: 5 52 4540 5
Size: 47 4488 2081
Fragments arranged by size:
4488 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6616
StyI C'CwwG_G
Cuts at: 513 1065 2072 3753 3867 4055 513
Size: 552 1007 1681 114 188 3074
Fragments arranged by size:
3074 1681 1007 552 188 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3284 3800 4643 4660 4813 4998 5443
Size: 1255 1704 516 843 17 153 185 445
Cuts at: 5443 325
Size: 1498
Fragments arranged by size:
1704 1498 1255 843 516 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4862 6574 96
Size: 274 80 33 51 1443 2885 1712 138
Fragments arranged by size:
2885 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6576 6608 6576
Size: 32 6584
Tth111I GACn'n_nGTC
Cuts at: 1316 2046 2828 1316
Size: 730 782 5104
Fragments arranged by size:
5104 782 730
XbaI T'CTAG_A
Cuts at: 3952 3952
Size: 6616
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3526 3526
Size: 6616
XhoI C'TCGA_G
Cuts at: 1943 3413 3888 1943
Size: 1470 475 4671
Fragments arranged by size:
4671 1470 475
XmaI C'CCGG_G
Cuts at: 1870 2906 3491 3941 1870
Size: 1036 585 450 4545
Fragments arranged by size:
4545 1036 585 450
XmnI GAAnn'nnTTC
Cuts at: 2297 4745 2297
Size: 2448 4168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4424 276
Size: 53 83 186 936 2890 2468
Fragments arranged by size:
2890 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BsiWI BstZ17I
DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI
NruI PacI PmlI PsrI RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI