(Linear) (Six-base) MAPSORT of: KIEE9692.seq Check: 1572 from: 1 to: 1911 KIEE9692.orf With 182 enzymes: SgfI * December 5, 2014 13:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1110 1553 1911 Size: 1110 443 358 Fragments arranged by size: 1110 443 358 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 746 1911 Size: 746 1165 AhdI GACnn_n'nnGTC Cuts at: 0 633 1911 Size: 633 1278 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1106 1911 Size: 1106 805 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1586 1618 1911 Size: 1586 32 293 Fragments arranged by size: 1586 293 32 AlwNI CAG_nnn'CTG Cuts at: 0 787 862 1360 1911 Size: 787 75 498 551 Fragments arranged by size: 787 551 498 75 ApaI G_GGCC'C Cuts at: 0 771 806 880 1076 1243 1696 1711 1911 Size: 771 35 74 196 167 453 15 200 Fragments arranged by size: 771 453 200 196 167 74 35 15 AvaI C'yCGr_G Cuts at: 0 901 1000 1408 1486 1883 1911 Size: 901 99 408 78 397 28 Fragments arranged by size: 901 408 397 99 78 28 BanI G'GyrC_C Cuts at: 0 546 890 1263 1567 1777 1911 Size: 546 344 373 304 210 134 Fragments arranged by size: 546 373 344 304 210 134 BanII G_rGCy'C Cuts at: 0 535 753 771 806 880 922 1059 1076 Size: 535 218 18 35 74 42 137 17 Cuts at: 1076 1243 1487 1494 1696 1711 1911 Size: 167 244 7 202 15 200 Fragments arranged by size: 535 244 218 202 200 167 137 74 42 35 18 17 15 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 337 1828 1911 Size: 337 1491 83 Fragments arranged by size: 1491 337 83 BclI T'GATC_A Cuts at: 0 1769 1911 Size: 1769 142 BfrBI ATG'CAT Cuts at: 0 1312 1911 Size: 1312 599 BglI GCCn_nnn'nGGC Cuts at: 0 801 1080 1255 1764 1911 Size: 801 279 175 509 147 Fragments arranged by size: 801 509 279 175 147 BlpI GC'TnA_GC Cuts at: 0 1493 1911 Size: 1493 418 Bme1580I G_kGCm'C Cuts at: 0 510 551 771 806 880 1076 1243 1570 Size: 510 41 220 35 74 196 167 327 Cuts at: 1570 1696 1711 1780 1911 Size: 126 15 69 131 Fragments arranged by size: 510 327 220 196 167 131 126 74 69 41 35 15 BmrI ACTGGGnnnn_n' Cuts at: 0 306 883 1181 1911 Size: 306 577 298 730 Fragments arranged by size: 730 577 306 298 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 55 1911 Size: 55 1856 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 186 448 566 818 851 1081 1316 1462 Size: 186 262 118 252 33 230 235 146 Cuts at: 1462 1911 Size: 449 Fragments arranged by size: 449 262 252 235 230 186 146 118 33 Bpu10I CC'TnA_GC Cuts at: 0 515 1024 1661 1911 Size: 515 509 637 250 Fragments arranged by size: 637 515 509 250 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1349 1911 Size: 1349 562 BsaI GGTCTCn'nnnn_ Cuts at: 0 439 1397 1532 1724 1911 Size: 439 958 135 192 187 Fragments arranged by size: 958 439 192 187 135 BsaHI Gr'CG_yC Cuts at: 0 1637 1911 Size: 1637 274 BsaWI w'CCGG_w Cuts at: 0 1303 1817 1911 Size: 1303 514 94 Fragments arranged by size: 1303 514 94 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 213 243 809 839 1721 1751 1911 Size: 213 30 566 30 882 30 160 Fragments arranged by size: 882 566 213 160 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 145 232 761 826 984 1911 Size: 145 87 529 65 158 927 Fragments arranged by size: 927 529 158 145 87 65 BseYI C'CCAG_C Cuts at: 0 480 837 932 943 1069 1711 1754 1830 Size: 480 357 95 11 126 642 43 76 Cuts at: 1830 1911 Size: 81 Fragments arranged by size: 642 480 357 126 95 81 76 43 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 559 1143 1911 Size: 559 584 768 Fragments arranged by size: 768 584 559 BsiHKAI G_wGCw'C Cuts at: 0 1030 1911 Size: 1030 881 BsmI GAATG_Cn' Cuts at: 0 1502 1911 Size: 1502 409 BsmBI CGTCTCn'nnnn_ Cuts at: 0 204 812 1911 Size: 204 608 1099 Fragments arranged by size: 1099 608 204 Bsp1286I G_dGCh'C Cuts at: 0 510 535 551 753 771 806 880 922 Size: 510 25 16 202 18 35 74 42 Cuts at: 922 1030 1059 1076 1243 1487 1494 1570 1696 Size: 108 29 17 167 244 7 76 126 Cuts at: 1696 1711 1780 1911 Size: 15 69 131 Fragments arranged by size: 510 244 202 167 131 126 108 76 74 69 42 35 29 25 18 17 16 15 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1110 1553 1911 Size: 1110 443 358 Fragments arranged by size: 1110 443 358 BsrBI CCG'CTC Cuts at: 0 1274 1911 Size: 1274 637 BsrFI r'CCGG_y Cuts at: 0 236 1763 1911 Size: 236 1527 148 Fragments arranged by size: 1527 236 148 BstAPI GCAn_nnn'nTGC Cuts at: 0 505 546 1911 Size: 505 41 1365 Fragments arranged by size: 1365 505 41 BstBI TT'CG_AA Cuts at: 0 1649 1911 Size: 1649 262 BstXI CCAn_nnnn'nTGG Cuts at: 0 480 951 1044 1911 Size: 480 471 93 867 Fragments arranged by size: 867 480 471 93 BstYI r'GATC_y Cuts at: 0 226 1911 Size: 226 1685 Bsu36I CC'TnA_GG Cuts at: 0 27 128 855 1911 Size: 27 101 727 1056 Fragments arranged by size: 1056 727 101 27 BtgI C'CryG_G Cuts at: 0 369 473 1911 Size: 369 104 1438 Fragments arranged by size: 1438 369 104 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 999 1241 1911 Size: 999 242 670 Fragments arranged by size: 999 670 242 BtsI GCAGTG_nn' Cuts at: 0 561 1748 1911 Size: 561 1187 163 Fragments arranged by size: 1187 561 163 DrdI GACnn_nn'nnGTC Cuts at: 0 600 1911 Size: 600 1311 EaeI y'GGCC_r Cuts at: 0 693 1386 1702 1745 1911 Size: 693 693 316 43 166 Fragments arranged by size: 693 693 316 166 43 EarI CTCTTCn'nnn_ Cuts at: 0 344 1043 1911 Size: 344 699 868 Fragments arranged by size: 868 699 344 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 594 1911 Size: 594 1317 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 186 448 566 746 818 851 1081 1316 Size: 186 262 118 180 72 33 230 235 Cuts at: 1316 1462 1911 Size: 146 449 Fragments arranged by size: 449 262 235 230 186 180 146 118 72 33 EcoNI CCTnn'n_nnAGG Cuts at: 0 466 612 854 866 1110 1911 Size: 466 146 242 12 244 801 Fragments arranged by size: 801 466 244 242 146 12 EcoO109I rG'GnC_Cy Cuts at: 0 90 151 380 456 486 767 802 803 Size: 90 61 229 76 30 281 35 1 Cuts at: 803 859 877 1145 1240 1357 1693 1708 1788 Size: 56 18 268 95 117 336 15 80 Cuts at: 1788 1867 1890 1911 Size: 79 23 21 Fragments arranged by size: 336 281 268 229 117 95 90 80 79 76 61 56 35 30 23 21 18 15 1 HaeII r_GCGC'y Cuts at: 0 416 1809 1911 Size: 416 1393 102 Fragments arranged by size: 1393 416 102 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 619 651 809 841 1501 1533 1911 Size: 619 32 158 32 660 32 378 Fragments arranged by size: 660 619 378 158 32 32 32 HincII GTy'rAC Cuts at: 0 250 1911 Size: 250 1661 HindIII A'AGCT_T Cuts at: 0 1796 1911 Size: 1796 115 MscI TGG'CCA Cuts at: 0 695 1388 1747 1911 Size: 695 693 359 164 Fragments arranged by size: 695 693 359 164 MslI CAynn'nnrTG Cuts at: 0 1724 1911 Size: 1724 187 MspA1I CmG'CkG Cuts at: 0 435 1126 1911 Size: 435 691 785 Fragments arranged by size: 785 691 435 NaeI GCC'GGC Cuts at: 0 238 1765 1911 Size: 238 1527 146 Fragments arranged by size: 1527 238 146 NgoMIV G'CCGG_C Cuts at: 0 236 1763 1911 Size: 236 1527 148 Fragments arranged by size: 1527 236 148 NsiI A_TGCA'T Cuts at: 0 1314 1911 Size: 1314 597 NspI r_CATG'y Cuts at: 0 1723 1911 Size: 1723 188 PasI CC'CwG_GG Cuts at: 0 450 915 1911 Size: 450 465 996 Fragments arranged by size: 996 465 450 PfoI T'CCnGG_A Cuts at: 0 325 423 464 867 1478 1911 Size: 325 98 41 403 611 433 Fragments arranged by size: 611 433 403 325 98 41 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1391 1423 1911 Size: 1391 32 488 Fragments arranged by size: 1391 488 32 PpuMI rG'GwC_Cy Cuts at: 0 90 151 456 486 1357 1788 1890 1911 Size: 90 61 305 30 871 431 102 21 Fragments arranged by size: 871 431 305 102 90 61 30 21 PshAI GACnn'nnGTC Cuts at: 0 1325 1911 Size: 1325 586 PspOMI G'GGCC_C Cuts at: 0 767 802 876 1072 1239 1692 1707 1911 Size: 767 35 74 196 167 453 15 204 Fragments arranged by size: 767 453 204 196 167 74 35 15 PspXI vC'TCGA_Gb Cuts at: 0 1408 1883 1911 Size: 1408 475 28 Fragments arranged by size: 1408 475 28 PstI C_TGCA'G Cuts at: 0 1560 1911 Size: 1560 351 PvuII CAG'CTG Cuts at: 0 435 1911 Size: 435 1476 SanDI GG'GwC_CC Cuts at: 0 90 456 486 1890 1911 Size: 90 366 30 1404 21 Fragments arranged by size: 1404 366 90 30 21 SfcI C'TryA_G Cuts at: 0 1244 1556 1911 Size: 1244 312 355 Fragments arranged by size: 1244 355 312 SmaI CCC'GGG Cuts at: 0 903 1488 1911 Size: 903 585 423 Fragments arranged by size: 903 585 423 SmlI C'TyrA_G Cuts at: 0 1364 1408 1883 1911 Size: 1364 44 475 28 Fragments arranged by size: 1364 475 44 28 StyI C'CwwG_G Cuts at: 0 67 1748 1862 1911 Size: 67 1681 114 49 Fragments arranged by size: 1681 114 67 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1279 1795 1911 Size: 1279 516 116 Fragments arranged by size: 1279 516 116 Tth111I GACn'n_nGTC Cuts at: 0 41 823 1911 Size: 41 782 1088 Fragments arranged by size: 1088 782 41 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1521 1911 Size: 1521 390 XhoI C'TCGA_G Cuts at: 0 1408 1883 1911 Size: 1408 475 28 Fragments arranged by size: 1408 475 28 XmaI C'CCGG_G Cuts at: 0 901 1486 1911 Size: 901 585 425 Fragments arranged by size: 901 585 425 XmnI GAAnn'nnTTC Cuts at: 0 292 1911 Size: 292 1619 Enzymes that do cut: AarI AcuI AhdI AlfI AloI AlwNI ApaI AvaI BanI BanII BbsI BclI BfrBI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII MscI MslI MspA1I NaeI NgoMIV NsiI NspI PasI PfoI PpiI PpuMI PshAI PspOMI PspXI PstI PvuII SanDI SfcI SmaI SmlI StyI TaqII Tth111I XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AleI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BglII BmgBI BmtI BsaAI BsaBI BsiEI BsiWI BspEI BspHI BsrDI BsrGI BssHII BssSI BstEII BstZ17I ClaI CspCI DraI DraIII EagI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HpaI KasI KpnI MfeI MluI MmeI NarI NcoI NdeI NheI NotI NruI PacI PciI PflMI PmeI PmlI PsiI PsrI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI XbaI ZraI