################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AlwNI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrBI BssSI Bst1107I BstAPI BstBI BstZ17I CelII ClaI CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT14I FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I MaeI MfeI MluI MluNI MroI MscI MseI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StyI SwaI TfiI Tru9I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AfaI 187 GTAC GT^AC Alw44I 822 GTGCAC G^TGCAC ApaI 668 GGGCCC GGGCC^C ApaLI 822 GTGCAC G^TGCAC AvaI 756 CCCGGG C^[CT]CG[GA]G BclI 833 TGATCA T^GATCA BpmI 136 CTGGAG CTGGAG(16/14) BsaBI 305 GATATACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 340 GAGACC GGTCTC(1/5) BsmFI 698 GTCCC GGGAC(10/14) BsoBI 756 CCCGGG C^[CT]CG[GA]G Bsp1407I 186 TGTACA T^GTACA BspMI 503 ACCTGC ACCTGC(4/8) BsrDI 867 CATTGC GCAATG(2/0) BsrFI 154 ACCGGC [GA]^CCGG[CT] BsrGI 186 TGTACA T^GTACA BssHII 180 GCGCGC G^CGCGC BstEII 384 GGTGACC G^GT[ACGT]ACC BstPI 384 GGTGACC G^GT[ACGT]ACC BstYI 986 AGATCC [GA]^GATC[CT] Cfr10I 154 ACCGGC [GA]^CCGG[CT] DraII 782 AGGACCT [GA]G^G[ACGT]CC[CT] DrdI 964 GACGAGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 111 CCGCGG C^C[GA][CT]GG EaeI 41 TGGCCG [CT]^GGCC[GA] EcoO109I 782 AGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 384 GGTGACC G^GT[ACGT]ACC EcoT22I 1087 ATGCAT ATGCA^T FbaI 833 TGATCA T^GATCA KspI 111 CCGCGG CCGC^GG MamI 305 GATATACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 986 AGATCC [GA]^GATC[CT] NsiI 1087 ATGCAT ATGCA^T PflMI 1068 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 782 AGGACCT [GA]G^G[AT]CC[CT] RsaI 187 GTAC GT^AC SacI 469 GAGCTC GAGCT^C SacII 111 CCGCGG CCGC^GG SmaI 756 CCCGGG CCC^GGG SspBI 186 TGTACA T^GTACA StuI 140 AGGCCT AGG^CCT Van91I 1068 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 927 CCAGGAGTTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 986 AGATCC [GA]^GATC[CT] XmaCI 756 CCCGGG C^CCGGG XmaI 756 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AocI 456 CCTCAGG CC^T[ACGT]AGG 786 CCTGAGG AspHI 469 GAGCTC G[AT]GC[AT]^C 822 GTGCAC BanII 469 GAGCTC G[GA]GC[CT]^C 668 GGGCCC BbeI 24 GGCGCC GGCGC^C 429 GGCGCC BbvI 360 GCAGC GCAGC(8/12) 287 GCTGC BsgI 288 CTGCAC GTGCAG(16/14) 358 GTGCAG BsiHKAI 469 GAGCTC G[AT]GC[AT]^C 822 GTGCAC BsmAI 340 GAGAC GTCTC(1/5) 973 GTCTC BstXI 293 CCATTTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 434 CCACATACTTGG Bsu36I 456 CCTCAGG CC^T[ACGT]AGG 786 CCTGAGG Eco81I 456 CCTCAGG CC^T[ACGT]AGG 786 CCTGAGG HgaI 603 GACGC GACGC(5/10) 1024 GACGC HinfI 652 GAGTC G^A[ACGT]TC 946 GACTC HphI 384 GGTGA GGTGA(8/7) 570 GGTGA KasI 24 GGCGCC G^GCGCC 429 GGCGCC MaeII 67 ACGT A^CGT 377 ACGT MaeIII 231 GTCAC ^GT[ACGT]AC 385 GTGAC MslI 684 CATCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 825 CACTGGGATG NarI 24 GGCGCC GG^CGCC 429 GGCGCC PleI 946 GACTC GAGTC(4/5) 652 GAGTC PvuII 285 CAGCTG CAG^CTG 392 CAGCTG SfoI 24 GGCGCC GGC^GCC 429 GGCGCC TaqI 64 TCGA T^CGA 209 TCGA TseI 287 GCTGC G^C[AT]GC 360 GCAGC Tsp45I 231 GTCAC ^GT[GC]AC 385 GTGAC Tsp509I 121 AATT ^AATT 523 AATT TthHB8I 64 TCGA T^CGA 209 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AvaII BanI BbsI BcnI BmyI BpuAI BsaHI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TspRI