(Circular) (Six-base) MAPSORT of: pFN21AE4352.seq Check: 8369 from: 1 to: 5812 pFN21AE4352 5812 bp With 182 enzymes: SgfI * March 28, 2012 13:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3622 278 Size: 53 83 186 936 2086 2468 Fragments arranged by size: 2468 2086 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3155 1937 Size: 1218 4594 Acc65I G'GTAC_C Cuts at: 3133 3133 Size: 5812 AclI AA'CG_TT Cuts at: 1563 3939 4312 1563 Size: 2376 373 3063 Fragments arranged by size: 3063 2376 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3875 5259 5527 1632 Size: 2243 1384 268 1917 Fragments arranged by size: 2243 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5615 AflIII A'CryG_T Cuts at: 1203 4711 5688 1203 Size: 3508 977 1327 Fragments arranged by size: 3508 1327 977 AgeI A'CCGG_T Cuts at: 4643 4643 Size: 5812 AhdI GACnn_n'nnGTC Cuts at: 4541 4541 Size: 5812 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5812 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2220 2889 1184 Size: 1036 669 4107 Fragments arranged by size: 4107 1036 669 AlwNI CAG_nnn'CTG Cuts at: 3555 5127 3555 Size: 1572 4240 ApaI G_GGCC'C Cuts at: 2677 2677 Size: 5812 ApaLI G'TGCA_C Cuts at: 2827 3869 5025 2827 Size: 1042 1156 3614 Fragments arranged by size: 3614 1156 1042 ApoI r'AATT_y Cuts at: 1493 3121 3347 3499 5608 1493 Size: 1628 226 152 2109 1697 Fragments arranged by size: 2109 1697 1628 226 152 AseI AT'TA_AT Cuts at: 160 1032 4366 160 Size: 872 3334 1606 Fragments arranged by size: 3334 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5812 AvaI C'yCGr_G Cuts at: 1870 1943 2761 3137 1870 Size: 73 818 376 4545 Fragments arranged by size: 4545 818 376 73 BamHI G'GATC_C Cuts at: 3142 3509 3142 Size: 367 5445 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2029 2153 2434 2515 2957 Size: 325 217 627 242 124 281 81 442 Cuts at: 2957 3133 4588 618 Size: 176 1455 1842 Fragments arranged by size: 1842 1455 627 442 325 281 242 217 176 124 81 BanII G_rGCy'C Cuts at: 1391 2478 2677 3131 1391 Size: 1087 199 454 4072 Fragments arranged by size: 4072 1087 454 199 BbeI G_GCGC'C Cuts at: 2033 2438 2033 Size: 405 5407 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2253 2421 3034 928 Size: 971 354 168 613 3706 Fragments arranged by size: 3706 971 613 354 168 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4003 4037 1903 Size: 34 2066 34 3678 Fragments arranged by size: 3678 2066 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3704 4914 3704 Size: 1210 4602 BclI T'GATC_A Cuts at: 1548 2838 1548 Size: 1290 4522 BfrBI ATG'CAT Cuts at: 3094 3094 Size: 5812 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4423 136 Size: 107 122 71 631 605 2751 1525 Fragments arranged by size: 2751 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5807 5807 Size: 5812 BlpI GC'TnA_GC Cuts at: 3229 3229 Size: 5812 Bme1580I G_kGCm'C Cuts at: 1790 2520 2677 2831 2962 3873 5029 1790 Size: 730 157 154 131 911 1156 2573 Fragments arranged by size: 2573 1156 911 730 157 154 131 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5812 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2680 2841 3543 Size: 416 53 83 399 24 1263 161 702 Cuts at: 3543 4501 442 Size: 958 1753 Fragments arranged by size: 1753 1263 958 702 416 399 161 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5812 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2995 2995 Size: 5812 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2162 4472 1239 Size: 923 2310 2579 Fragments arranged by size: 2579 2310 923 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3937 4802 5100 3937 Size: 865 298 4649 Fragments arranged by size: 4649 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2339 4475 882 Size: 617 840 2136 2219 Fragments arranged by size: 2219 2136 840 617 BsaAI yAC'GTr Cuts at: 493 1204 3603 493 Size: 711 2399 2702 Fragments arranged by size: 2702 2399 711 BsaBI GATnn'nnATC Cuts at: 2314 5806 2314 Size: 3492 2320 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2030 2435 2609 3030 Size: 53 83 186 936 497 405 174 421 Cuts at: 3030 3619 4001 4636 275 Size: 589 382 635 1451 Fragments arranged by size: 1451 936 635 589 497 421 405 382 186 174 83 53 BsaWI w'CCGG_w Cuts at: 4245 4643 4917 5064 5475 4245 Size: 398 274 147 411 4582 Fragments arranged by size: 4582 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5587 Fragments arranged by size: 5587 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5812 BseYI C'CCAG_C Cuts at: 2632 2725 5015 5511 2632 Size: 93 2290 496 2933 Fragments arranged by size: 2933 2290 496 93 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2278 2384 1163 Size: 573 542 106 4591 Fragments arranged by size: 4591 573 542 106 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3262 4023 5051 1540 Size: 275 186 1261 761 1028 2301 Fragments arranged by size: 2301 1261 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2478 2831 3873 3958 5029 2478 Size: 353 1042 85 1071 3261 Fragments arranged by size: 3261 1071 1042 353 85 BsmI GAATG_Cn' Cuts at: 3323 3416 3323 Size: 93 5719 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2478 2520 2677 2831 2962 3131 3873 Size: 399 688 42 157 154 131 169 742 Cuts at: 3873 3958 5029 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 688 399 169 157 154 131 85 42 BspEI T'CCGG_A Cuts at: 5475 5475 Size: 5812 BspHI T'CATG_A Cuts at: 3701 5431 3701 Size: 1730 4082 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2517 3167 844 Size: 1673 650 3489 Fragments arranged by size: 3489 1673 650 BsrBI CCG'CTC Cuts at: 1394 1823 3699 1394 Size: 429 1876 3507 Fragments arranged by size: 3507 1876 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2871 4307 4481 65 Size: 1183 1623 1436 174 1396 Fragments arranged by size: 1623 1436 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2159 4456 4643 1700 Size: 110 349 2297 187 2869 Fragments arranged by size: 2869 2297 349 187 110 BsrGI T'GTAC_A Cuts at: 96 2191 96 Size: 2095 3717 BssHII G'CGCG_C Cuts at: 1925 2185 1925 Size: 260 5552 BssSI C'ACGA_G Cuts at: 3872 4884 3872 Size: 1012 4800 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5812 BstBI TT'CG_AA Cuts at: 4616 4616 Size: 5812 BstEII G'GTnAC_C Cuts at: 1184 2389 1184 Size: 1205 4607 BstXI CCAn_nnnn'nTGG Cuts at: 2305 2446 2305 Size: 141 5671 BstYI r'GATC_y Cuts at: 1972 2991 3142 3509 3894 3911 5363 5449 5461 Size: 1019 151 367 385 17 1452 86 12 Cuts at: 5461 5807 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1019 385 367 346 151 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2462 2792 2462 Size: 330 5482 BtgI C'CryG_G Cuts at: 513 1065 2004 2116 513 Size: 552 939 112 4209 Fragments arranged by size: 4209 939 552 112 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5073 Fragments arranged by size: 5073 683 56 BtsI GCAGTG_nn' Cuts at: 2135 3328 4122 4142 2135 Size: 1193 794 20 3805 Fragments arranged by size: 3805 1193 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5812 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5777 DraI TTT'AAA Cuts at: 3116 3116 Size: 5812 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2975 4819 809 Size: 507 1659 1844 1802 Fragments arranged by size: 1844 1802 1659 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 3259 4148 Size: 54 1425 50 275 18 216 1213 889 Cuts at: 4148 5777 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1213 889 275 216 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3259 1537 Size: 275 1447 4090 Fragments arranged by size: 4090 1447 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3742 1353 Size: 2389 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4399 4773 4919 387 Size: 1064 138 2810 374 146 1280 Fragments arranged by size: 2810 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2476 2476 Size: 5812 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2162 3875 4472 5259 5527 1239 Size: 393 530 1713 597 787 268 1524 Fragments arranged by size: 1713 1524 787 597 530 393 268 EcoO109I rG'GnC_Cy Cuts at: 2788 2788 Size: 5812 EcoRI G'AATT_C Cuts at: 3121 3499 3121 Size: 378 5434 FspI TGC'GCA Cuts at: 4318 4318 Size: 5812 HaeII r_GCGC'y Cuts at: 1678 2033 2045 2438 4959 1678 Size: 355 12 393 2521 2531 Fragments arranged by size: 2531 2521 393 355 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4453 4485 4527 4559 1373 Size: 32 135 32 2881 32 42 32 2626 Fragments arranged by size: 2881 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3156 3402 669 Size: 988 281 1218 246 3079 Fragments arranged by size: 3079 1218 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5812 HpaI GTT'AAC Cuts at: 3402 3402 Size: 5812 KasI G'GCGC_C Cuts at: 2029 2434 2029 Size: 405 5407 KpnI G_GTAC'C Cuts at: 3137 3137 Size: 5812 MfeI C'AATT_G Cuts at: 3411 3411 Size: 5812 MluI A'CGCG_T Cuts at: 5688 5688 Size: 5812 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2343 4926 5110 1305 Size: 1038 2583 184 2007 Fragments arranged by size: 2583 2007 1038 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3990 Fragments arranged by size: 3990 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2693 2834 3770 4129 4288 518 Size: 648 1527 141 936 359 159 2042 Fragments arranged by size: 2042 1527 936 648 359 159 141 MspA1I CmG'CkG Cuts at: 1606 2036 2118 2292 2399 3228 3905 5053 5298 Size: 430 82 174 107 829 677 1148 245 Cuts at: 5298 1606 Size: 2120 Fragments arranged by size: 2120 1148 829 677 430 245 174 107 82 NarI GG'CG_CC Cuts at: 2030 2435 2030 Size: 405 5407 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4321 Fragments arranged by size: 4321 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5812 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5812 NotI GC'GGCC_GC Cuts at: 3259 3259 Size: 5812 NsiI A_TGCA'T Cuts at: 3096 3096 Size: 5812 NspI r_CATG'y Cuts at: 1132 1229 3170 4715 1132 Size: 97 1941 1545 2229 Fragments arranged by size: 2229 1941 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5812 PciI A'CATG_T Cuts at: 4711 4711 Size: 5812 PflMI CCAn_nnn'nTGG Cuts at: 1108 3079 1108 Size: 1971 3841 PfoI T'CCnGG_A Cuts at: 2478 2772 2478 Size: 294 5518 PmeI GTTT'AAAC Cuts at: 3116 3116 Size: 5812 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3881 3913 5394 5426 3881 Size: 32 1481 32 4267 Fragments arranged by size: 4267 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2788 2788 Size: 5812 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5812 PsiI TTA'TAA Cuts at: 3382 3382 Size: 5812 PspOMI G'GGCC_C Cuts at: 2673 2673 Size: 5812 PstI C_TGCA'G Cuts at: 830 3164 830 Size: 2334 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5812 PvuII CAG'CTG Cuts at: 2292 2399 2292 Size: 107 5705 SacI G_AGCT'C Cuts at: 2478 2478 Size: 5812 SacII CC_GC'GG Cuts at: 2119 2119 Size: 5812 SalI G'TCGA_C Cuts at: 1936 3154 1936 Size: 1218 4594 SbfI CC_TGCA'GG Cuts at: 3164 3164 Size: 5812 ScaI AGT'ACT Cuts at: 4060 5772 4060 Size: 1712 4100 SfcI C'TryA_G Cuts at: 826 1045 3160 4295 4976 5167 826 Size: 219 2115 1135 681 191 1471 Fragments arranged by size: 2115 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2031 2436 2031 Size: 405 5407 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5812 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5812 SmaI CCC'GGG Cuts at: 1872 2763 3139 1872 Size: 891 376 4545 Fragments arranged by size: 4545 891 376 SmlI C'TyrA_G Cuts at: 820 1017 1943 3916 4817 5079 820 Size: 197 926 1973 901 262 1553 Fragments arranged by size: 1973 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3603 493 Size: 3110 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5812 SphI G_CATG'C Cuts at: 1132 3170 1132 Size: 2038 3774 SspI AAT'ATT Cuts at: 5 52 3736 5 Size: 47 3684 2081 Fragments arranged by size: 3684 2081 47 StuI AGG'CCT Cuts at: 1517 2147 1517 Size: 630 5182 StyI C'CwwG_G Cuts at: 513 1065 2004 3251 513 Size: 552 939 1247 3074 Fragments arranged by size: 3074 1247 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2105 3839 3856 4009 4194 4639 325 Size: 1255 525 1734 17 153 185 445 1498 Fragments arranged by size: 1734 1498 1255 525 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2191 4058 5770 Size: 274 80 33 51 1443 214 1867 1712 Cuts at: 5770 96 Size: 138 Fragments arranged by size: 1867 1712 1443 274 214 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5772 5804 5772 Size: 32 5780 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5812 XbaI T'CTAG_A Cuts at: 3148 3148 Size: 5812 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2939 2939 Size: 5812 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5812 XmaI C'CCGG_G Cuts at: 1870 2761 3137 1870 Size: 891 376 4545 Fragments arranged by size: 4545 891 376 XmnI GAAnn'nnTTC Cuts at: 3941 3941 Size: 5812 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3620 276 Size: 53 83 186 936 2086 2468 Fragments arranged by size: 2468 2086 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI