(Circular) (Six-base) MAPSORT of: pFN21AE1809.seq Check: 9454 from: 1 to: 5680 pFN21AE1809 5680 bp With 182 enzymes: SgfI * January 30, 2014 17:20 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3490 278 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3023 1937 Size: 1086 4594 Acc65I G'GTAC_C Cuts at: 3001 3001 Size: 5680 AclI AA'CG_TT Cuts at: 1563 3807 4180 1563 Size: 2244 373 3063 Fragments arranged by size: 3063 2244 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2880 3743 5127 5395 1632 Size: 1248 863 1384 268 1917 Fragments arranged by size: 1917 1384 1248 863 268 AfeI AGC'GCT Cuts at: 2390 2390 Size: 5680 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5483 AflIII A'CryG_T Cuts at: 1203 2010 4579 5556 1203 Size: 807 2569 977 1327 Fragments arranged by size: 2569 1327 977 807 AgeI A'CCGG_T Cuts at: 4511 4511 Size: 5680 AhdI GACnn_n'nnGTC Cuts at: 4409 4409 Size: 5680 AleI CACnn'nnGTG Cuts at: 1166 2234 1166 Size: 1068 4612 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5680 AlwNI CAG_nnn'CTG Cuts at: 2185 2743 3423 4995 2185 Size: 558 680 1572 2870 Fragments arranged by size: 2870 1572 680 558 ApaLI G'TGCA_C Cuts at: 3737 4893 3737 Size: 1156 4524 ApoI r'AATT_y Cuts at: 1493 2788 2989 3215 3367 5476 1493 Size: 1295 201 226 152 2109 1697 Fragments arranged by size: 2109 1697 1295 226 201 152 AseI AT'TA_AT Cuts at: 160 1032 4234 160 Size: 872 3202 1606 Fragments arranged by size: 3202 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 AvaI C'yCGr_G Cuts at: 1870 1943 2139 2529 2932 3005 1870 Size: 73 196 390 403 73 4545 Fragments arranged by size: 4545 403 390 196 73 73 BamHI G'GATC_C Cuts at: 3010 3377 3010 Size: 367 5313 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2066 2811 3001 4456 618 Size: 325 217 627 279 745 190 1455 1842 Fragments arranged by size: 1842 1455 745 627 325 279 217 190 BanII G_rGCy'C Cuts at: 1391 2999 1391 Size: 1608 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2868 928 Size: 971 969 3740 Fragments arranged by size: 3740 971 969 BbvCI CC'TCA_GC Cuts at: 2507 2507 Size: 5680 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3871 3905 1903 Size: 34 1934 34 3678 Fragments arranged by size: 3678 1934 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3572 4782 3572 Size: 1210 4470 BclI T'GATC_A Cuts at: 1548 1548 Size: 5680 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2524 4291 136 Size: 107 122 71 631 605 852 1767 1525 Fragments arranged by size: 1767 1525 852 631 605 122 107 71 BglII A'GATC_T Cuts at: 2379 5675 2379 Size: 3296 2384 BlpI GC'TnA_GC Cuts at: 3097 3097 Size: 5680 Bme1580I G_kGCm'C Cuts at: 1790 2313 3741 4897 1790 Size: 523 1428 1156 2573 Fragments arranged by size: 2573 1428 1156 523 BmgBI CAC'GTC Cuts at: 1318 2536 1318 Size: 1218 4462 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2113 2879 3411 Size: 416 53 83 399 24 696 766 532 Cuts at: 3411 4369 442 Size: 958 1753 Fragments arranged by size: 1753 958 766 696 532 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2327 1056 Size: 1271 4409 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2914 2914 Size: 5680 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4340 1239 Size: 3101 2579 Bpu10I CC'TnA_GC Cuts at: 2507 2507 Size: 5680 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2552 3805 4670 4968 2552 Size: 1253 865 298 3264 Fragments arranged by size: 3264 1253 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2261 4343 882 Size: 617 762 2082 2219 Fragments arranged by size: 2219 2082 762 617 BsaAI yAC'GTr Cuts at: 493 1204 2320 2602 2770 3471 493 Size: 711 1116 282 168 701 2702 Fragments arranged by size: 2702 1116 711 701 282 168 BsaBI GATnn'nnATC Cuts at: 2730 5674 2730 Size: 2944 2736 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3487 3869 4504 275 Size: 53 83 186 936 1954 382 635 1451 Fragments arranged by size: 1954 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4113 4511 4785 4932 5343 4113 Size: 398 274 147 411 4450 Fragments arranged by size: 4450 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5455 Fragments arranged by size: 5455 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5680 BseYI C'CCAG_C Cuts at: 2402 2783 4883 5379 2402 Size: 381 2100 496 2703 Fragments arranged by size: 2703 2100 496 381 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2198 2211 2405 1163 Size: 573 462 13 194 4438 Fragments arranged by size: 4438 573 462 194 13 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3130 3891 4919 1540 Size: 275 186 1129 761 1028 2301 Fragments arranged by size: 2301 1129 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2646 3741 3826 4897 2646 Size: 1095 85 1071 3429 Fragments arranged by size: 3429 1095 1071 85 BsmI GAATG_Cn' Cuts at: 3191 3284 3191 Size: 93 5587 BsmBI CGTCTCn'nnnn_ Cuts at: 2526 2526 Size: 5680 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2313 2646 2999 3741 3826 4897 1391 Size: 399 523 333 353 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 523 399 353 333 85 BspEI T'CCGG_A Cuts at: 5343 5343 Size: 5680 BspHI T'CATG_A Cuts at: 3569 5299 3569 Size: 1730 3950 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2751 3035 844 Size: 1907 284 3489 Fragments arranged by size: 3489 1907 284 BsrBI CCG'CTC Cuts at: 1394 1823 2295 3567 1394 Size: 429 472 1272 3507 Fragments arranged by size: 3507 1272 472 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2356 4175 4349 65 Size: 1183 1108 1819 174 1396 Fragments arranged by size: 1819 1396 1183 1108 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4324 4511 1700 Size: 110 2514 187 2869 Fragments arranged by size: 2869 2514 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5680 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5680 BssSI C'ACGA_G Cuts at: 3740 4752 3740 Size: 1012 4668 BstAPI GCAn_nnn'nTGC Cuts at: 794 2743 794 Size: 1949 3731 BstBI TT'CG_AA Cuts at: 4484 4484 Size: 5680 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5680 BstYI r'GATC_y Cuts at: 1972 2181 2379 2430 3010 3377 3762 3779 5231 Size: 209 198 51 580 367 385 17 1452 Cuts at: 5231 5317 5329 5675 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 580 385 367 346 209 198 86 51 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2094 513 Size: 552 939 90 4099 Fragments arranged by size: 4099 939 552 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4941 Fragments arranged by size: 4941 683 56 BtsI GCAGTG_nn' Cuts at: 2656 2681 2703 3196 3990 4010 2656 Size: 25 22 493 794 20 4326 Fragments arranged by size: 4326 794 493 25 22 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5680 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5645 DraI TTT'AAA Cuts at: 2984 2984 Size: 5680 DraIII CAC_nnn'GTG Cuts at: 2486 2873 2486 Size: 387 5293 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2446 4687 809 Size: 507 1130 2241 1802 Fragments arranged by size: 2241 1802 1130 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2148 2454 2586 Size: 54 1425 50 275 18 318 306 132 Cuts at: 2586 2614 2678 2688 2719 3127 4016 5645 8 Size: 28 64 10 31 408 889 1629 43 Fragments arranged by size: 1629 1425 889 408 318 306 275 132 64 54 50 43 31 28 18 10 EagI C'GGCC_G Cuts at: 1537 1812 3127 1537 Size: 275 1315 4090 Fragments arranged by size: 4090 1315 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3610 1353 Size: 2257 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4267 4641 4787 387 Size: 1064 138 2678 374 146 1280 Fragments arranged by size: 2678 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2880 3743 4340 5127 5395 1239 Size: 393 1248 863 597 787 268 1524 Fragments arranged by size: 1524 1248 863 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2113 2526 2824 2113 Size: 413 298 4969 Fragments arranged by size: 4969 413 298 EcoRI G'AATT_C Cuts at: 2989 3367 2989 Size: 378 5302 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2962 2962 Size: 5680 FspI TGC'GCA Cuts at: 4186 4186 Size: 5680 HaeII r_GCGC'y Cuts at: 1678 2024 2392 4827 1678 Size: 346 368 2435 2531 Fragments arranged by size: 2531 2435 368 346 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2270 2302 4321 4353 4395 Size: 32 135 32 698 32 2019 32 42 Cuts at: 4395 4427 1373 Size: 32 2626 Fragments arranged by size: 2626 2019 698 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3024 3270 669 Size: 988 281 1086 246 3079 Fragments arranged by size: 3079 1086 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5680 HpaI GTT'AAC Cuts at: 3270 3270 Size: 5680 KpnI G_GTAC'C Cuts at: 3005 3005 Size: 5680 MfeI C'AATT_G Cuts at: 3279 3279 Size: 5680 MluI A'CGCG_T Cuts at: 5556 5556 Size: 5680 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4794 4978 1305 Size: 3489 184 2007 Fragments arranged by size: 3489 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2150 2456 2616 2690 10 Size: 54 1425 343 318 306 160 74 3000 Fragments arranged by size: 3000 1425 343 318 306 160 74 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2234 2462 3638 3997 4156 518 Size: 648 843 225 228 1176 359 159 2042 Fragments arranged by size: 2042 1176 843 648 359 228 225 159 MspA1I CmG'CkG Cuts at: 1606 2557 2578 3096 3773 4921 5166 1606 Size: 951 21 518 677 1148 245 2120 Fragments arranged by size: 2120 1148 951 677 518 245 21 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4189 Fragments arranged by size: 4189 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5680 NheI G'CTAG_C Cuts at: 1052 2323 1052 Size: 1271 4409 NotI GC'GGCC_GC Cuts at: 3127 3127 Size: 5680 NspI r_CATG'y Cuts at: 1132 1229 2014 3038 4583 1132 Size: 97 785 1024 1545 2229 Fragments arranged by size: 2229 1545 1024 785 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5680 PciI A'CATG_T Cuts at: 2010 4579 2010 Size: 2569 3111 PflMI CCAn_nnn'nTGG Cuts at: 1108 2391 2662 1108 Size: 1283 271 4126 Fragments arranged by size: 4126 1283 271 PfoI T'CCnGG_A Cuts at: 2262 2262 Size: 5680 PmeI GTTT'AAAC Cuts at: 2984 2984 Size: 5680 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3749 3781 5262 5294 3749 Size: 32 1481 32 4135 Fragments arranged by size: 4135 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2113 2824 2113 Size: 711 4969 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5680 PsiI TTA'TAA Cuts at: 3250 3250 Size: 5680 PstI C_TGCA'G Cuts at: 830 2739 3032 830 Size: 1909 293 3478 Fragments arranged by size: 3478 1909 293 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5680 PvuII CAG'CTG Cuts at: 2557 2578 2557 Size: 21 5659 SalI G'TCGA_C Cuts at: 1936 3022 1936 Size: 1086 4594 SanDI GG'GwC_CC Cuts at: 2113 2113 Size: 5680 SbfI CC_TGCA'GG Cuts at: 3032 3032 Size: 5680 ScaI AGT'ACT Cuts at: 3928 5640 3928 Size: 1712 3968 SfcI C'TryA_G Cuts at: 826 1045 2735 3028 4163 4844 5035 826 Size: 219 1690 293 1135 681 191 1471 Fragments arranged by size: 1690 1471 1135 681 293 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5680 SmaI CCC'GGG Cuts at: 1872 2141 3007 1872 Size: 269 866 4545 Fragments arranged by size: 4545 866 269 SmlI C'TyrA_G Cuts at: 820 1017 1943 2567 3784 4685 4947 820 Size: 197 926 624 1217 901 262 1553 Fragments arranged by size: 1553 1217 926 901 624 262 197 SnaBI TAC'GTA Cuts at: 493 3471 493 Size: 2978 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5680 SphI G_CATG'C Cuts at: 1132 3038 1132 Size: 1906 3774 SspI AAT'ATT Cuts at: 5 52 3604 5 Size: 47 3552 2081 Fragments arranged by size: 3552 2081 47 StuI AGG'CCT Cuts at: 1517 2930 1517 Size: 1413 4267 StyI C'CwwG_G Cuts at: 513 1065 2004 2157 2344 3119 513 Size: 552 939 153 187 775 3074 Fragments arranged by size: 3074 939 775 552 187 153 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2087 2177 2861 3707 3724 3877 4062 Size: 1255 507 90 684 846 17 153 185 Cuts at: 4062 4507 325 Size: 445 1498 Fragments arranged by size: 1498 1255 846 684 507 445 185 153 90 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3926 5638 96 Size: 274 80 33 51 1443 1949 1712 138 Fragments arranged by size: 1949 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5640 5672 5640 Size: 32 5648 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5680 XbaI T'CTAG_A Cuts at: 3016 3016 Size: 5680 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2342 2449 2464 2342 Size: 107 15 5558 Fragments arranged by size: 5558 107 15 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5680 XmaI C'CCGG_G Cuts at: 1870 2139 3005 1870 Size: 269 866 4545 Fragments arranged by size: 4545 866 269 XmnI GAAnn'nnTTC Cuts at: 3809 3809 Size: 5680 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3488 276 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI