Restriction map for
pFN21AE3063
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BcgI BciVI BclI
BfaI BfrI BglI BlnI BlpI
BmyI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
CpoI DraI DraII DraIII DrdI
Eam1105I EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MluNI MroI MscI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccI 190 GTCTAC GT^[AC][GT]AC
AccII 126 CGCG CG^CG
AfaI 158 GTAC GT^AC
AflIII 426 ACGTGT A^C[GA][CT]GT
AlwI 209 GGATC GGATC(4/5)
AlwNI 642 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 245 AGCGCT AGC^GCT
BbsI 523 GTCTTC GAAGAC(2/6)
BbvCI 439 GCTGAGG CCTCAGC(-5/-2)
BglII 221 AGATCT A^GATCT
BpuAI 523 GTCTTC GAAGAC(2/6)
BsgI 337 GTGCAG GTGCAG(16/14)
BsiEI 611 CGGCCG CG[GA][CT]^CG
BsmAI 14 GAGAC GTCTC(1/5)
BspDI 169 ATCGAT AT^CGAT
BspMI 519 GCAGGT ACCTGC(4/8)
BstUI 126 CGCG CG^CG
BstYI 221 AGATCT [GA]^GATC[CT]
ClaI 169 ATCGAT AT^CGAT
EagI 611 CGGCCG C^GGCCG
EclXI 611 CGGCCG C^GGCCG
Eco47III 245 AGCGCT AGC^GCT
Eco52I 611 CGGCCG C^GGCCG
HphI 260 TCACC GGTGA(8/7)
MaeII 426 ACGT A^CGT
MaeIII 410 GTGAC ^GT[ACGT]AC
MflI 221 AGATCT [GA]^GATC[CT]
MvnI 126 CGCG CG^CG
NcoI 284 CCATGG C^CATGG
RsaI 158 GTAC GT^AC
ScaI 157 AGTACT AGT^ACT
TfiI 322 GAATC G^A[AT]TC
Tsp45I 410 GTGAC ^GT[GC]AC
XhoII 221 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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BcnI 276 CCGGG CC^[GC]GG
609 CCCGG
BsrI 344 ACTGG ACTGG(1/-1)
491 CCAGT
CfoI 246 GCGC GCG^C
531 GCGC
DdeI 440 CTGAG C^T[ACGT]AG
588 CTCAG
EaeI 447 TGGCCG [CT]^GGCC[GA]
611 CGGCCG
FauI 145 CCCGC CCCGC(4/6)
235 GCGGG
HaeII 245 AGCGCT [GA]GCGC^[CT]
530 AGCGCC
HhaI 246 GCGC GCG^C
531 GCGC
HinP1I 246 GCGC G^CGC
531 GCGC
MboII 555 GAAGA GAAGA(8/7)
524 TCTTC
MslI 390 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
507 CATCAATGTG
NciI 276 CCGGG CC^[GC]GG
609 CCCGG
NlaIV 280 GGAGCC GG[ACGT]^[ACGT]CC
573 GGAACC
PleI 133 GACTC GAGTC(4/5)
565 GAGTC
PstI 217 CTGCAG CTGCA^G
648 CTGCAG
SfcI 217 CTGCAG C^T[GA][CT]AG
648 CTGCAG
TspRI 93 CAGTG CA[GC]TG[ACGT][ACGT]^
492 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI BbvI BpmI BsaJI
BsiYI BslI BsmFI BssKI BstF5I
BstNI Cac8I Cfr13I DpnII DsaI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI MboI
MnlI MspA1I MspI MvaI MwoI
NdeII NlaIII PvuII Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TseI
TthHB8I