(Linear) (Six-base) MAPSORT of: KIEE3063.seq Check: 2850 from: 1 to: 657 KIEE3063.orf With 182 enzymes: SgfI * March 22, 2012 16:08 .. AccI GT'mk_AC Cuts at: 0 191 657 Size: 191 466 AfeI AGC'GCT Cuts at: 0 247 657 Size: 247 410 AflIII A'CryG_T Cuts at: 0 426 657 Size: 426 231 AlwNI CAG_nnn'CTG Cuts at: 0 647 657 Size: 647 10 BbsI GAAGACnn'nnnn_ Cuts at: 0 516 657 Size: 516 141 BbvCI CC'TCA_GC Cuts at: 0 440 657 Size: 440 217 BglII A'GATC_T Cuts at: 0 221 657 Size: 221 436 BmrI ACTGGGnnnn_n' Cuts at: 0 354 657 Size: 354 303 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 32 436 567 611 657 Size: 32 404 131 44 46 Fragments arranged by size: 404 131 46 44 32 Bpu10I CC'TnA_GC Cuts at: 0 440 588 657 Size: 440 148 69 Fragments arranged by size: 440 148 69 BseYI C'CCAG_C Cuts at: 0 577 635 640 657 Size: 577 58 5 17 Fragments arranged by size: 577 58 17 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 358 657 Size: 358 299 BsiEI CG_ry'CG Cuts at: 0 614 657 Size: 614 43 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 510 657 Size: 510 147 BstYI r'GATC_y Cuts at: 0 221 657 Size: 221 436 BtgI C'CryG_G Cuts at: 0 31 263 284 614 657 Size: 31 232 21 330 43 Fragments arranged by size: 330 232 43 31 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 294 657 Size: 294 363 BtsI GCAGTG_nn' Cuts at: 0 99 657 Size: 99 558 ClaI AT'CG_AT Cuts at: 0 170 657 Size: 170 487 EaeI y'GGCC_r Cuts at: 0 447 611 657 Size: 447 164 46 Fragments arranged by size: 447 164 46 EagI C'GGCC_G Cuts at: 0 611 657 Size: 611 46 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 32 436 567 611 657 Size: 32 404 131 44 46 Fragments arranged by size: 404 131 46 44 32 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 126 540 657 Size: 126 414 117 Fragments arranged by size: 414 126 117 HaeII r_GCGC'y Cuts at: 0 249 534 657 Size: 249 285 123 Fragments arranged by size: 285 249 123 MslI CAynn'nnrTG Cuts at: 0 394 511 657 Size: 394 117 146 Fragments arranged by size: 394 146 117 MspA1I CmG'CkG Cuts at: 0 10 235 334 647 653 657 Size: 10 225 99 313 6 4 Fragments arranged by size: 313 225 99 10 6 4 NcoI C'CATG_G Cuts at: 0 284 657 Size: 284 373 PstI C_TGCA'G Cuts at: 0 221 652 657 Size: 221 431 5 Fragments arranged by size: 431 221 5 PvuII CAG'CTG Cuts at: 0 10 647 653 657 Size: 10 637 6 4 Fragments arranged by size: 637 10 6 4 ScaI AGT'ACT Cuts at: 0 159 657 Size: 159 498 SfcI C'TryA_G Cuts at: 0 217 648 657 Size: 217 431 9 Fragments arranged by size: 431 217 9 StyI C'CwwG_G Cuts at: 0 284 468 497 657 Size: 284 184 29 160 Fragments arranged by size: 284 184 160 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 34 657 Size: 34 623 TatI w'GTAC_w Cuts at: 0 157 657 Size: 157 500 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 393 425 657 Size: 393 32 232 Fragments arranged by size: 393 232 32 Enzymes that do cut: AccI AfeI AflIII AlwNI BbsI BbvCI BglII BmrI BpmI Bpu10I BseYI BsgI BsiEI BspMI BstYI BtgI BtgZI BtsI ClaI EaeI EagI Eco57MI FalI HaeII MslI MspA1I NcoI PstI PvuII ScaI SfcI StyI TaqII TatI TstI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI