(Circular) (Six-base) MAPSORT of: pFN21AE2025.seq Check: 3283 from: 1 to: 6529 pFN21AE2025 6529 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2743 2743 Size: 6529 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2880 4339 278 Size: 53 83 186 936 1344 1459 2468 Fragments arranged by size: 2468 1459 1344 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3872 1937 Size: 1935 4594 Acc65I G'GTAC_C Cuts at: 2943 3249 3850 2943 Size: 306 601 5622 Fragments arranged by size: 5622 601 306 AclI AA'CG_TT Cuts at: 1563 4656 5029 1563 Size: 3093 373 3063 Fragments arranged by size: 3093 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2861 4592 5976 6244 1632 Size: 1229 1731 1384 268 1917 Fragments arranged by size: 1917 1731 1384 1229 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6332 AflIII A'CryG_T Cuts at: 1203 2856 3314 3489 5428 6405 1203 Size: 1653 458 175 1939 977 1327 Fragments arranged by size: 1939 1653 1327 977 458 175 AgeI A'CCGG_T Cuts at: 5360 5360 Size: 6529 AhdI GACnn_n'nnGTC Cuts at: 5258 5258 Size: 6529 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6529 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6529 AlwNI CAG_nnn'CTG Cuts at: 2170 4272 5844 2170 Size: 2102 1572 2855 Fragments arranged by size: 2855 2102 1572 ApaI G_GGCC'C Cuts at: 2079 2079 Size: 6529 ApaLI G'TGCA_C Cuts at: 4586 5742 4586 Size: 1156 5373 ApoI r'AATT_y Cuts at: 1493 3128 3838 4064 4216 6325 1493 Size: 1635 710 226 152 2109 1697 Fragments arranged by size: 2109 1697 1635 710 226 152 AseI AT'TA_AT Cuts at: 160 1032 5083 160 Size: 872 4051 1606 Fragments arranged by size: 4051 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6529 AvaI C'yCGr_G Cuts at: 1870 1943 2062 2177 2259 2719 2747 3854 1870 Size: 73 119 115 82 460 28 1107 4545 Fragments arranged by size: 4545 1107 460 119 115 82 73 28 BamHI G'GATC_C Cuts at: 2592 3042 3859 4226 2592 Size: 450 817 367 4895 Fragments arranged by size: 4895 817 450 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2105 2513 2943 2987 3249 Size: 325 217 627 318 408 430 44 262 Cuts at: 3249 3261 3698 3850 5305 618 Size: 12 437 152 1455 1842 Fragments arranged by size: 1842 1455 627 437 430 408 325 318 262 217 152 44 12 BanII G_rGCy'C Cuts at: 1391 2079 2778 3237 3662 3848 1391 Size: 688 699 459 425 186 4072 Fragments arranged by size: 4072 699 688 459 425 186 BbeI G_GCGC'C Cuts at: 2109 3702 2109 Size: 1593 4936 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2427 928 Size: 971 528 5030 Fragments arranged by size: 5030 971 528 BbvCI CC'TCA_GC Cuts at: 2670 3518 2670 Size: 848 5681 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4720 4754 1903 Size: 34 2783 34 3678 Fragments arranged by size: 3678 2783 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4421 5631 4421 Size: 1210 5319 BclI T'GATC_A Cuts at: 1548 1548 Size: 6529 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2283 5140 136 Size: 107 122 71 631 605 611 2857 1525 Fragments arranged by size: 2857 1525 631 611 605 122 107 71 BglII A'GATC_T Cuts at: 6524 6524 Size: 6529 BlpI GC'TnA_GC Cuts at: 3946 3946 Size: 6529 Bme1580I G_kGCm'C Cuts at: 1790 2079 3440 3733 4590 5746 1790 Size: 289 1361 293 857 1156 2573 Fragments arranged by size: 2573 1361 1156 857 293 289 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6529 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4260 5218 442 Size: 416 53 83 399 24 2843 958 1753 Fragments arranged by size: 2843 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6529 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2420 2640 2831 3571 5189 1239 Size: 1181 220 191 740 1618 2579 Fragments arranged by size: 2579 1618 1181 740 220 191 Bpu10I CC'TnA_GC Cuts at: 2670 3518 2670 Size: 848 5681 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3583 4654 5519 5817 3583 Size: 1071 865 298 4295 Fragments arranged by size: 4295 1071 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5192 882 Size: 617 3693 2219 Fragments arranged by size: 3693 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2503 4320 493 Size: 711 1299 1817 2702 Fragments arranged by size: 2702 1817 1299 711 BsaBI GATnn'nnATC Cuts at: 3116 6523 3116 Size: 3407 3122 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2106 2877 3699 3785 Size: 53 83 186 936 573 771 822 86 Cuts at: 3785 4336 4718 5353 275 Size: 551 382 635 1451 Fragments arranged by size: 1451 936 822 771 635 573 551 382 186 86 83 53 BsaWI w'CCGG_w Cuts at: 3039 4962 5360 5634 5781 6192 3039 Size: 1923 398 274 147 411 3376 Fragments arranged by size: 3376 1923 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2723 2753 3632 3662 1178 Size: 30 165 30 1320 30 879 30 4045 Fragments arranged by size: 4045 1320 879 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2105 2444 2747 1186 Size: 919 339 303 4968 Fragments arranged by size: 4968 919 339 303 BseYI C'CCAG_C Cuts at: 2475 2661 2846 5732 6228 2475 Size: 186 185 2886 496 2776 Fragments arranged by size: 2886 2776 496 186 185 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2293 2465 1163 Size: 573 557 172 5227 Fragments arranged by size: 5227 573 557 172 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3552 3979 4740 5768 1540 Size: 275 186 1551 427 761 1028 2301 Fragments arranged by size: 2301 1551 1028 761 427 275 186 BsiHKAI G_wGCw'C Cuts at: 2252 2626 3674 4590 4675 5746 2252 Size: 374 1048 916 85 1071 3035 Fragments arranged by size: 3035 1071 1048 916 374 85 BsmI GAATG_Cn' Cuts at: 3142 4040 4133 3142 Size: 898 93 5538 Fragments arranged by size: 5538 898 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2252 2626 2778 3237 3440 3662 Size: 399 289 173 374 152 459 203 222 Cuts at: 3662 3674 3733 3848 4590 4675 5746 1391 Size: 12 59 115 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 459 399 374 289 222 203 173 152 115 85 59 12 BspEI T'CCGG_A Cuts at: 3039 6192 3039 Size: 3153 3376 BspHI T'CATG_A Cuts at: 4418 6148 4418 Size: 1730 4799 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2390 2743 3884 844 Size: 1546 353 1141 3489 Fragments arranged by size: 3489 1546 1141 353 BsrBI CCG'CTC Cuts at: 1394 1823 2404 2422 4416 1394 Size: 429 581 18 1994 3507 Fragments arranged by size: 3507 1994 581 429 18 BsrDI GCAATG_nn' Cuts at: 65 1248 5024 5198 65 Size: 1183 3776 174 1396 Fragments arranged by size: 3776 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2189 3380 5173 5360 1700 Size: 110 379 1191 1793 187 2869 Fragments arranged by size: 2869 1793 1191 379 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6529 BssHII G'CGCG_C Cuts at: 1925 2185 2524 1925 Size: 260 339 5930 Fragments arranged by size: 5930 339 260 BssSI C'ACGA_G Cuts at: 3724 4589 5601 3724 Size: 865 1012 4652 Fragments arranged by size: 4652 1012 865 BstAPI GCAn_nnn'nTGC Cuts at: 794 2170 794 Size: 1376 5153 BstBI TT'CG_AA Cuts at: 5333 5333 Size: 6529 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6529 BstXI CCAn_nnnn'nTGG Cuts at: 2462 2656 3710 3781 2462 Size: 194 1054 71 5210 Fragments arranged by size: 5210 1054 194 71 BstYI r'GATC_y Cuts at: 1972 2142 2592 3042 3406 3859 4226 4611 4628 Size: 170 450 450 364 453 367 385 17 Cuts at: 4628 6080 6166 6178 6524 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 453 450 450 385 367 364 346 170 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2655 2778 2959 2991 3157 3534 Size: 552 939 651 123 181 32 166 377 Cuts at: 3534 3759 3780 513 Size: 225 21 3262 Fragments arranged by size: 3262 939 651 552 377 225 181 166 123 32 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2194 487 Size: 683 56 968 4822 Fragments arranged by size: 4822 968 683 56 BtsI GCAGTG_nn' Cuts at: 3574 3715 4045 4839 4859 3574 Size: 141 330 794 20 5244 Fragments arranged by size: 5244 794 330 141 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6529 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6494 DraI TTT'AAA Cuts at: 3833 3833 Size: 6529 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2431 5536 809 Size: 507 1115 3105 1802 Fragments arranged by size: 3105 1802 1115 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3051 3531 3976 Size: 54 1425 50 275 18 1221 480 445 Cuts at: 3976 4865 6494 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1221 889 480 445 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3976 1537 Size: 275 2164 4090 Fragments arranged by size: 4090 2164 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2678 2798 4459 1353 Size: 1325 120 1661 3423 Fragments arranged by size: 3423 1661 1325 120 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5116 5490 5636 387 Size: 1064 138 3527 374 146 1280 Fragments arranged by size: 3527 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2420 2640 2831 2861 3571 4592 5189 Size: 393 788 220 191 30 710 1021 597 Cuts at: 5189 5976 6244 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1021 788 787 710 597 393 268 220 191 30 EcoO109I rG'GnC_Cy Cuts at: 2075 2076 3690 2075 Size: 1 1614 4914 Fragments arranged by size: 4914 1614 1 EcoRI G'AATT_C Cuts at: 3838 4216 3838 Size: 378 6151 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2311 2953 2311 Size: 642 5887 FspI TGC'GCA Cuts at: 5035 5035 Size: 6529 HaeII r_GCGC'y Cuts at: 1678 2109 2171 3702 5676 1678 Size: 431 62 1531 1974 2531 Fragments arranged by size: 2531 1974 1531 431 62 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2723 2755 3290 3322 3622 Size: 32 135 32 1151 32 535 32 300 Cuts at: 3622 3654 5170 5202 5244 5276 1373 Size: 32 1516 32 42 32 2626 Fragments arranged by size: 2626 1516 1151 535 300 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3745 3873 4119 669 Size: 988 281 1807 128 246 3079 Fragments arranged by size: 3079 1807 988 281 246 128 HindIII A'AGCT_T Cuts at: 748 748 Size: 6529 HpaI GTT'AAC Cuts at: 4119 4119 Size: 6529 KasI G'GCGC_C Cuts at: 2105 3698 2105 Size: 1593 4936 KpnI G_GTAC'C Cuts at: 2947 3253 3854 2947 Size: 306 601 5622 Fragments arranged by size: 5622 601 306 MfeI C'AATT_G Cuts at: 4128 4128 Size: 6529 MluI A'CGCG_T Cuts at: 6405 6405 Size: 6529 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5643 5827 1305 Size: 4338 184 2007 Fragments arranged by size: 4338 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3053 10 Size: 54 1425 343 1221 3486 Fragments arranged by size: 3486 1425 1221 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2229 3779 4487 4846 5005 518 Size: 648 1063 1550 708 359 159 2042 Fragments arranged by size: 2042 1550 1063 708 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2012 2149 2850 2993 3525 3945 4622 5770 Size: 406 137 701 143 532 420 677 1148 Cuts at: 5770 6015 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 701 677 532 420 406 245 143 137 NaeI GCC'GGC Cuts at: 2191 2191 Size: 6529 NarI GG'CG_CC Cuts at: 2106 3699 2106 Size: 1593 4936 NcoI C'CATG_G Cuts at: 513 1065 2004 2655 2778 2959 3759 3780 513 Size: 552 939 651 123 181 800 21 3262 Fragments arranged by size: 3262 939 800 651 552 181 123 21 NdeI CA'TA_TG Cuts at: 387 387 Size: 6529 NgoMIV G'CCGG_C Cuts at: 2189 2189 Size: 6529 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6529 NotI GC'GGCC_GC Cuts at: 3976 3976 Size: 6529 NspI r_CATG'y Cuts at: 1132 1229 2791 2809 2860 3139 3318 3493 3887 Size: 97 1562 18 51 279 179 175 394 Cuts at: 3887 5432 1132 Size: 1545 2229 Fragments arranged by size: 2229 1562 1545 394 279 179 175 97 51 18 PasI CC'CwG_GG Cuts at: 1348 2308 3703 1348 Size: 960 1395 4174 Fragments arranged by size: 4174 1395 960 PciI A'CATG_T Cuts at: 2856 3314 3489 5428 2856 Size: 458 175 1939 3957 Fragments arranged by size: 3957 1939 458 175 PflMI CCAn_nnn'nTGG Cuts at: 1108 2398 2940 2965 3163 1108 Size: 1290 542 25 198 4474 Fragments arranged by size: 4474 1290 542 198 25 PfoI T'CCnGG_A Cuts at: 3402 3650 3402 Size: 248 6281 PmeI GTTT'AAAC Cuts at: 3833 3833 Size: 6529 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4598 4630 6111 6143 4598 Size: 32 1481 32 4984 Fragments arranged by size: 4984 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3690 3690 Size: 6529 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6529 PsiI TTA'TAA Cuts at: 4099 4099 Size: 6529 PspOMI G'GGCC_C Cuts at: 2075 2075 Size: 6529 PspXI vC'TCGA_Gb Cuts at: 2177 2177 Size: 6529 PstI C_TGCA'G Cuts at: 830 3881 830 Size: 3051 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6529 RsrII CG'GwC_CG Cuts at: 2701 2701 Size: 6529 SacII CC_GC'GG Cuts at: 2994 2994 Size: 6529 SalI G'TCGA_C Cuts at: 1936 3871 1936 Size: 1935 4594 SapI GCTCTTCn'nnn_ Cuts at: 2798 2798 Size: 6529 SbfI CC_TGCA'GG Cuts at: 3881 3881 Size: 6529 ScaI AGT'ACT Cuts at: 2450 4777 6489 2450 Size: 2327 1712 2490 Fragments arranged by size: 2490 2327 1712 SexAI A'CCwGG_T Cuts at: 3252 3252 Size: 6529 SfcI C'TryA_G Cuts at: 826 1045 2218 3877 5012 5693 5884 826 Size: 219 1173 1659 1135 681 191 1471 Fragments arranged by size: 1659 1471 1173 1135 681 219 191 SfoI GGC'GCC Cuts at: 2107 3700 2107 Size: 1593 4936 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6529 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6529 SmaI CCC'GGG Cuts at: 1872 2064 2721 3856 1872 Size: 192 657 1135 4545 Fragments arranged by size: 4545 1135 657 192 SmlI C'TyrA_G Cuts at: 820 1017 1943 2177 3562 4633 5534 5796 820 Size: 197 926 234 1385 1071 901 262 1553 Fragments arranged by size: 1553 1385 1071 926 901 262 234 197 SnaBI TAC'GTA Cuts at: 493 4320 493 Size: 3827 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6529 SphI G_CATG'C Cuts at: 1132 2809 3887 1132 Size: 1677 1078 3774 Fragments arranged by size: 3774 1677 1078 SspI AAT'ATT Cuts at: 5 52 4453 5 Size: 47 4401 2081 Fragments arranged by size: 4401 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6529 StyI C'CwwG_G Cuts at: 513 1065 2004 2655 2778 2928 2959 3288 3685 Size: 552 939 651 123 150 31 329 397 Cuts at: 3685 3759 3780 3968 513 Size: 74 21 188 3074 Fragments arranged by size: 3074 939 651 552 397 329 188 150 123 74 31 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2359 3659 4556 4573 4726 4911 5356 Size: 1255 779 1300 897 17 153 185 445 Cuts at: 5356 325 Size: 1498 Fragments arranged by size: 1498 1300 1255 897 779 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2448 3066 4775 Size: 274 80 33 51 1443 471 618 1709 Cuts at: 4775 6487 96 Size: 1712 138 Fragments arranged by size: 1712 1709 1443 618 471 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3476 3508 3632 3664 6489 6521 3476 Size: 32 124 32 2825 32 3484 Fragments arranged by size: 3484 2825 124 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6529 XbaI T'CTAG_A Cuts at: 3865 3865 Size: 6529 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3400 3710 3400 Size: 310 6219 XhoI C'TCGA_G Cuts at: 1943 2177 1943 Size: 234 6295 XmaI C'CCGG_G Cuts at: 1870 2062 2719 3854 1870 Size: 192 657 1135 4545 Fragments arranged by size: 4545 1135 657 192 XmnI GAAnn'nnTTC Cuts at: 4658 4658 Size: 6529 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2878 4337 276 Size: 53 83 186 936 1344 1459 2468 Fragments arranged by size: 2468 1459 1344 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PsrI PvuII SacI SanDI SfiI SrfI SwaI